Chromatographia最新文献

{"title":"A Certified Reference Material of Polyvinyl Chloride Containing Phthalic Esters (NMIJ CRM 8156-a) for Screening Device Applications","authors":"Nobuyasu Hanari,&nbsp;Yukari Orihara,&nbsp;Yoshie Aoyagi,&nbsp;Shigetomo Matsuyama","doi":"10.1007/s10337-023-04297-6","DOIUrl":"10.1007/s10337-023-04297-6","url":null,"abstract":"<div><p>Phthalic esters (PEs) are used as plasticizers for plastic products especially for polyvinyl chloride (PVC). Owing to their adverse effects on human health, certain types of PEs are subjected to regulatory measures. Accurate and precision analyses of such regulated PEs are essential for monitoring compliance with regulatory standards; thus, the use of certified reference materials (CRMs) for quality assurance is recommended. To quantify the PE content of PVC products, the National Metrology Institute of Japan has developed a CRM, designated as NMIJ CRM 8156-a. This CRM is designed for validating the analytical methods and screening devices at low concentration levels (ca. 0.01%). Isotope-dilution mass spectrometry, the primary methods of measurement, has been employed for certification in conjunction with optimized gas and liquid chromatography/mass spectrometry techniques. Based on the mean mass fractions of target PEs derived from these two methods, eight certified values were determined for the CRM. The uncertainties associated with these certified values were estimated considering various factors such as characterization, homogeneity, and stability. Furthermore, the NMIJ CRMs were tested in screening devices such as pyrolyzers and thermal desorption mass spectrometers, confirming their suitability for generating calibration curves as well as the semi-quantitative analysis of target PEs. In summary, these CRMs are effective for validating quantitative and screening analytical methods for PEs in PVC and related substances.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 1","pages":"35 - 43"},"PeriodicalIF":1.2,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138506471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Coumarin-Based Phototoxins in Citrus-Derived Essential Oils Using Liquid Chromatography-Mass Spectrometry","authors":"Siheng Li,&nbsp;Colleen Kelly,&nbsp;Radim Knob,&nbsp;Kent McConnell,&nbsp;Justin Barrett","doi":"10.1007/s10337-023-04298-5","DOIUrl":"10.1007/s10337-023-04298-5","url":null,"abstract":"<div><p>The occurrence of coumarin-based photoactive compounds in essential oils has been a concern due to the adverse health effects it has on human health. A fit-for-purpose analytical method for major coumarin-based phototoxins is critical to guarantee the essential oils product safety and compliance with the regulatory requirements. Herein, a liquid chromatography-mass spectrometry (LC–MS) analytical method was developed to identify and quantify 25 coumarin derivatives in essential oils. The sample preparation procedure was optimized to use selective solvent extraction to remove the limonene matrix interference. To achieve the optimal sensitivity and specificity, UHPLC column of biphenyl stationary phase was used for this analysis. An inter-laboratory validation study was conducted to evaluate the method performance. The limit of quantification (LOQ) for all target analytes was validated at 5 µg/g. Satisfactory recoveries were obtained for the majority of analytes in the range of 80–115%. Both the repeatability RSD_r (within-laboratory) and reproducibility RSD_R (between-laboratory) were &lt; 10%. This method was demonstrated as applicable for the quality control testing of coumarin-based phototoxins in essential oils.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 1","pages":"59 - 69"},"PeriodicalIF":1.2,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138541916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical Composition Analysis and Multi-index Component Content Determination of Compounds in Goupi Plaster Based on UPLC–Q-Exactive-MS and UPLC–MS/MS","authors":"Yunfeng Xue,&nbsp;Tong Guan,&nbsp;Jia Liu,&nbsp;Jiajing Wang,&nbsp;Zhixin Yang,&nbsp;Feng Guan,&nbsp;Weinan Li,&nbsp;Yanhong Wang","doi":"10.1007/s10337-023-04296-7","DOIUrl":"10.1007/s10337-023-04296-7","url":null,"abstract":"<div><h3>Objective</h3><p>This study sought to evaluate and validate a method for chemical composition analysis and content determination of Goupi plaster components, a conventional prescription preparation of traditional Chinese medicine. This is geared toward providing a basis for quality control research and future development of Goupi plaster.</p><h3>Methods</h3><p>UPLC–Q-Exactive-MS was used to qualitatively analyze the chemical components of Goupi plaster from different manufacturers in positive and negative ion modes. UPLC–MS/MS method was used to establish the determination methods for the detection of sinomenine, osthole, and cinnamaldehyde in Goupi plaster from different manufacturers.</p><h3>Results</h3><p>A total of 291 chemical components were identified in Goupi plaster from four manufacturers, including 97 chemical components with known source and pharmacological activity. Further, we determined the contents of sinomenine, osthole, and cinnamaldehyde.</p><h3>Conclusion</h3><p>In summary, the UPLC–Q-Exactive-MS method was used to analyze the chemical components of Goupi plaster from different manufacturers. We established the UPLC–MS/MS method to determine the contents of sinomenine, osthole, and cinnamaldehyde in Goupi plaster from different manufacturers. The findings indicated that the method was comprehensive, rapid, and accurate, preliminarily revealing the material basis of Goupi plaster and providing a reference for follow-up development of Goupi plaster.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 1","pages":"45 - 57"},"PeriodicalIF":1.2,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10337-023-04296-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138506476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forced Degradation of an Anticancer Drug Apalutamide: Impurity Profiling and Structure Elucidation Study","authors":"Rekha Sapkal,&nbsp;Sadaf Quadri,&nbsp;Rupali Mahajan,&nbsp;Hara Prasad Padhy,&nbsp;Amit Asthana,&nbsp;Gananadhamu Samanthula,&nbsp;Amol G. Dikundwar","doi":"10.1007/s10337-023-04294-9","DOIUrl":"10.1007/s10337-023-04294-9","url":null,"abstract":"<div><p>An anticancer drug apalutamide approved by United States Food and Drug Administration in 2018 is one of the most commonly prescribed chemotherapeutic agents used for the treatment of prostate cancer. The present study aims at establishing the chemical structures of all the degradation products of the active pharmaceutical ingredient (API) formed under different forced degradation conditions. Apalutamide degradation was studied under International Council for Harmonisation recommended conditions of hydrolysis (acidic, alkaline, and neutral), oxidation, photolysis, and thermal stress. In total, seven degradation products (DP-1 to DP-7) were observed which were successfully separated on high-performance liquid chromatography and further characterized using liquid chromatography–mass spectrometry quadrupole time-of-flight (LC–MS/MS-QTOF). Degradation pathways for each of the observed DPs have been proposed based on the mass fragmentation pattern of API as well as DPs, identifying the underlying chemical transformations. Prediction of DPs was performed with Zeneth software tool, and the results were compared with the experimental observations. In silico toxicity assessment carried out using Derek suite reveals toxic nature of some of these DPs.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 1","pages":"71 - 82"},"PeriodicalIF":1.2,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135192572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Study of Cellulose Tris(3-chloro-5-methylphenylcarbamate) Coated or Covalently Immobilized on Silica for the Separation of Enantiomers in High-Performance Liquid Chromatography","authors":"Tamar Khatiashvili,&nbsp;Iza Matarashvili,&nbsp;Marina Karchkhadze,&nbsp;Tivadar Farkas,&nbsp;Bezhan Chankvetadze","doi":"10.1007/s10337-023-04295-8","DOIUrl":"10.1007/s10337-023-04295-8","url":null,"abstract":"<div><p>In the present study, a comparison was performed between chiral stationary phases (CSPs) prepared by coating or covalent immobilization of cellulose tris(3-chloro-5-methylphenylcarbamate) onto silica. The columns were evaluated in HPLC separation of enantiomers of various acidic, basic, and neutral chiral analytes in acetonitrile, methanol, and mixtures of n-hexane and propan-2-ol as mobile phases. A difference in enantiomer-resolving ability of coated and covalently immobilized CSs was observed.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 1","pages":"27 - 34"},"PeriodicalIF":1.2,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135476790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HPLC Retention Behavior of Oligonucleotides on an Anion-Exchange HPLC with a High Concentration of Urea","authors":"Kunio Kawamura,&nbsp;Yoshimi Maruoka","doi":"10.1007/s10337-023-04291-y","DOIUrl":"10.1007/s10337-023-04291-y","url":null,"abstract":"<div><p>Roles of short RNA molecules in the emergence process of primitive life-like system and physiological activities have been extensively studied from the viewpoint of both fundamental and applicational areas. The discovery of catalytic functions of short RNA molecules suggests that the most primitive life-like systems have emerged from a chemical network consisting of short functional RNA molecules that existed. However, the quantitative analysis of such short RNA, including ribozymes, micro-RNA, and small non-coding RNA consisting of less than approximately 100 nucleotide units is difficult to perform using conventional techniques, such as gel electrophoresis (GE) and high-performance liquid chromatography (HPLC). Both GE and HPLC are complementarily used for different purposes related to oligonucleotide analysis. Recently, the HPLC method’s usefulness with a high urea concentration has been demonstrated for short oligonucleotides. In this study, the HPLC separation behavior in the presence of a high urea concentration is studied using a Tosoh DNA-NPR anion-exchange column (diameter 2.0 mm, length 100 mm along with a guard column) with a buffer containing 0.02 M Tris and 7.5 M urea at pH 9.0 and a buffer containing 1.5 M NaCl, 0.02 M Tris, and 7.5 M urea at pH 9.0 mixed with a linear gradient at a flow rate of 0.15 mL min⁻¹ at 35 °C. Different sequences of oligonucleotides are collected to know how the influence of secondary structure formation can be reduced. Molecular modeling of the secondary structure is applied to evaluate the HPLC retention behaviors of oligonucleotides on an anion-exchange column. This study provides valuable information for selecting the elution conditions for separating the RNA and DNA oligonucleotides and preheating treatment.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 1","pages":"17 - 25"},"PeriodicalIF":1.2,"publicationDate":"2023-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10337-023-04291-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135774238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Support Vector Models-Based Quantitative Structure–Retention Relationship (QSRR) in the Development and Validation of RP-HPLC Method for Multi-component Analysis of Anti-diabetic Drugs","authors":"Krishnapal Rajput,&nbsp;Shubham Dhiman,&nbsp;N. Krishna Veni,&nbsp;V. Ravichandiran,&nbsp;Ramalingam Peraman","doi":"10.1007/s10337-023-04292-x","DOIUrl":"10.1007/s10337-023-04292-x","url":null,"abstract":"<div><p>This work emphasized the use of the quantitative structure–retention relationship (QSRR) approach in the prediction retention time of anti-diabetic drugs on C₁₈ column in the HPLC method development process. This in silico QSRR study utilized a data set from literature and in-house studies for the development of better predictive model. A total of 11 QSRR models were developed and narrowed to 5 candidate models using a mobile phase composition range. The candidate models 1, 2, 3, 4, and 5 showed <i>R</i>² scores of 0.8844, 0.8968, 0.8996, 0.9769, and 0.9916, respectively. The model validation data revealed that support vector model (SVM)-based models 4 and 5 showed better predictive ability (&gt; 99%) than the random forest model. The <i>R</i>² value for capacity factor prediction for models 4 and 5 was 0.862 and 0.881, respectively. Accordingly, the experimental retention time of pioglitazone, glimepiride, gliclazide, glyburide, and metformin was experimentally verified. Accordingly, we demonstrated good correlation (<i>R</i>² &gt; 0.9) between experimental and predictive retention time on C₁₈ column. Based on prediction, a new HPLC method was optimized for the simultaneous analysis of pioglitazone (3.6 ± 0.2 min) and glimepiride (6.1 ± 0.2 min) on C₁₈ column using a mobile phase consisting of methanol and 0.1% <i>ortho</i> phosphoric acid (pH 2.7) with detection at 227 nm. The respective % retention prediction error was 0.2% and 6.3% for pioglitazone and glimepiride. The method demonstrated the linearity with regression coefficients of 0.9985 and 0.9998, respectively, for pioglitazone (15–75 µg/mL) and glimepiride (2–10 µg/mL). The % RSD (0.77–1.43%) and % accuracy (98.01–102.39%) of the method were acceptable. The method has proven specificity in the presence of degradation products and demonstrated robustness (&lt; 2%RSD) to critical method parameters.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 1","pages":"3 - 16"},"PeriodicalIF":1.2,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135819965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetic, Protein Binding, and Tissue Distribution Investigations of Viloxazine Enantiomers in Rat Plasma by HPLC–MS/MS Using Polysaccharide-Based Immobilized Chiral Column: A Preclinical Approach to Possible Chiral Switch","authors":"V. V. S. Prasanna Kumari Rayala,&nbsp;Samir Ranjan Panda,&nbsp;P. Radhakrishnanand","doi":"10.1007/s10337-023-04293-w","DOIUrl":"10.1007/s10337-023-04293-w","url":null,"abstract":"<div><p>Viloxazine has a long past of medical use to treat depression in Europe, and it has lately been repurposed for the treatment of attention-deficit/hyperactivity disorder in the USA in an extended-release formulation. For the first time in this study, viloxazine enantiomers in rat plasma (both male and female) were determined using a sensitive and enantioselective HPLC–MS/MS approach. The enantio-separation was carried out using a Chiralpak IC column with a mobile phase consisting of 10 mM ammonium bicarbonate: methanol (5:95% <i>v</i>/<i>v</i>). The chosen internal standard was lamivudine. The mass detection in multiple reaction monitoring mode (MRM) was used to identify both viloxazine enantiomers and the internal standard with a positive electrospray ionization source. The transitions for viloxazine enantiomers and lamivudine were detected at <i>m</i>/<i>z</i> 238.2 → 100.0 and 229.9 → 112.0, respectively. The lower limit of quantification was 1 ng mL⁻¹, and the technique was verified through a validation varied from 1 to 2000 ng mL⁻¹ in rat plasma and 1–500 ng mg⁻¹ in heart, liver, kidney, and brain tissue for individual viloxazine enantiomers. The intra-day and inter-day precision's relative standard deviations were 5.57% in rat plasma and 5.86% in tissues, while the accuracy's relative errors varied from -12.92% to 11.85%. The validated method was applied to the pharmacokinetic and tissue distribution study of viloxazine enantiomers by following oral administration of 10 mg kg⁻¹ to individual enantiomers and racemic viloxazine to both male and female Sprague Dawley rats. It was established that the pharmacokinetic profile of S-Viloxazine was not statistically different from R-Viloxazine.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"86 11-12","pages":"751 - 764"},"PeriodicalIF":1.7,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71909158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DoE Guided Development of an HPLC Method for Evaluation of Amoxicillin and Metronidazole Co-loaded Mucoadhesive GRDDS Formulation for H. pylori Eradication","authors":"Ashutosh Gupta,&nbsp;Prerana D. Navti,&nbsp;Srinivas Mutalik,&nbsp;Moumita Saha,&nbsp;Sudheer Moorkoth","doi":"10.1007/s10337-023-04290-z","DOIUrl":"10.1007/s10337-023-04290-z","url":null,"abstract":"<div><p><i>H. Pylori</i> infection is a major reason for chronic Peptic Ulcer Disease (PUD) and related complications worldwide. Traditional treatment options fail to eradicate the organism completely which leads to antibiotic resistance. Novel formulations are being developed with an aim for effective treatment. Mucoadhesive GRDDS system is an option to increase the antibiotic bioavailability. The objective of this work is to develop a RP-HPLC–PDA based analytical method to evaluate the entrapment efficiency of the GRDDS systems developed for <i>H. pylori</i> treatment containing amoxicillin and metronidazole. DoE was used for optimizing the HPLC conditions. The optimized method used a HyperClone ODS C₁₈ column as stationary phase and methanol: phosphate buffer (15:85) at pH 6.4 as the mobile phase. The flow rate was 0.9 mL/min. Method was validated as per the ICH Q2(R1) guideline. Method was linear from 0.5 to 20 µg/ml for both amoxicillin and metronidazole with an R² value of 0.9995 and 0.9996 respectively. The validated HPLC method showed specificity for both drugs in the presence of degradation products as well as from other excipients of the GRDDS system. The validated method was applied to determine the entrapment efficiency as a part of formulation optimization. The entrapment efficiency of the final formulation was determined to be 78–84% and 75–82% for amoxicillin and metronidazole respectively.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"86 11-12","pages":"729 - 742"},"PeriodicalIF":1.7,"publicationDate":"2023-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71910641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Simultaneous Determination of Cyanide, Azide, and Ethanol in Whole Blood Using Headspace Gas Chromatography-Mass Spectrometry","authors":"Takaya Murakami,&nbsp;Yoshiaki Iwamuro,&nbsp;Yuki Sakamoto,&nbsp;Eriko Minami,&nbsp;Reiko Ishimaru,&nbsp;Hitoshi Tsuchihashi,&nbsp;Satoshi Chinaka","doi":"10.1007/s10337-023-04289-6","DOIUrl":"10.1007/s10337-023-04289-6","url":null,"abstract":"<div><p>The rapid detection and quantification of toxic substances and alcohol in biological specimens stand as imperative tasks, particularly in instances of unnatural demise and poisoning incidents. In this study, we developed a high-throughput simultaneous method for analyzing cyanide, azide, and ethanol in whole blood. This methodology involves a simple manual pretreatment step, entailing the sequential addition of an internal standard, ascorbic acid, and phosphoric acid to whole blood samples (within 1 min), followed by headspace gas chromatography-mass spectrometry analysis (within 14 min). The method exhibits high linearity (correlation coefficients, &gt; 0.9996), sensitivities of ng/mL-level detection, and satisfactory precision (relative standard deviation of less than 5.8% for cyanide, 8.8% for azide, and 2.9% for ethanol) and trueness (relative error within 7.3% for cyanide, 17% for azide, and 6.9% for ethanol). The proposed method, which does not require complex analytical techniques and possesses several advantages such as rapidity, simplicity, and high sensitivity and selectivity, is expected to contribute to the practical routine analysis in forensic and toxicological fields.</p><h3>Graphical Abstract</h3>\u0000 <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\u0000 </div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"86 11-12","pages":"701 - 706"},"PeriodicalIF":1.7,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71910763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}