New Comb-Like Polyelectrolytes in Capillary Electrophoresis

IF 1.2 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2024-11-28 DOI:10.1007/s10337-024-04376-2

Aleksandra A. Adamova, Mikhail S. Orlov, Nadezhda S. Rakovskaya, Petr A. Fetin, Lyudmila A. Kartsova

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Abstract

New comb-like polyelectrolytes with triethylammonium (pAUTEA-Br), N-methyl-piperidinium (pAUMP-Br), N-methyl-morpholinium (pAUMM-Br) and pyridinium (pAUPy-Br) cation groups were first used to separate steroid hormones, biogenic amines and amino acids under capillary electrophoresis conditions. The polymers acted as modifiers of electrophoretic systems and were used as dynamic coatings of inner surface of fused-silica capillary and additives to background electrolyte to perform capillary electrochromatography and micellar electrokinetic chromatography regimes, leading to increase in separation selectivity and efficiency, and as reagents for indirect spectrophotometric detection of amino acids. New materials also showed activity during on-line preconcentration of biogenic amines and reduced their limits of detections by 10 times. Thus, new comb-like polyelectrolytes were demonstrated to act as multifunctional materials for capillary electrophoresis. This study discusses the features of the influence of the polyelectrolytes on electrophoretic separation capabilities and their possibilities in separation of model biologically active substances. Modifiers of this type significantly expand the analytical capabilities of the capillary electrophoresis method.

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.