Chromatographia最新文献

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Congress, Conferences, and Workshops 大会、会议和研讨会
IF 1.2 4区 化学
Chromatographia Pub Date : 2024-06-03 DOI: 10.1007/s10337-024-04343-x
{"title":"Congress, Conferences, and Workshops","authors":"","doi":"10.1007/s10337-024-04343-x","DOIUrl":"10.1007/s10337-024-04343-x","url":null,"abstract":"","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 7-8","pages":"529 - 530"},"PeriodicalIF":1.2,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142407420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comprehensive Quantification of Miconazole Nitrate, Mupirocin, and Mometasone Furoate: a Dual Analysis via HPLC and HPTLC with Comparative Evaluation Against Greenness Parameters 硝酸咪康唑、莫匹罗星和糠酸莫美他松的全面定量:通过 HPLC 和 HPTLC 进行双重分析,并与绿色度参数进行比较评估
IF 1.2 4区 化学
Chromatographia Pub Date : 2024-05-18 DOI: 10.1007/s10337-024-04338-8
Aarti Sachin Zanwar, Anuj N. Nahata, Ashim Kumar Sen, Dhanya B. Sen, Sachin Zanwar, Mital Patel
{"title":"Comprehensive Quantification of Miconazole Nitrate, Mupirocin, and Mometasone Furoate: a Dual Analysis via HPLC and HPTLC with Comparative Evaluation Against Greenness Parameters","authors":"Aarti Sachin Zanwar,&nbsp;Anuj N. Nahata,&nbsp;Ashim Kumar Sen,&nbsp;Dhanya B. Sen,&nbsp;Sachin Zanwar,&nbsp;Mital Patel","doi":"10.1007/s10337-024-04338-8","DOIUrl":"10.1007/s10337-024-04338-8","url":null,"abstract":"<div><p>Combination treatment enhances efficacy, minimizes side effects, and improves convenience for patients with diverse treatment schedules. This compilation focuses on the simultaneous estimation of Miconazole nitrate (MNE), Mupirocin (MRN), and Mometasone furoate (MMT) using HPLC and HPTLC in an ointment which is wisely used combination for treatment of skin disorders. The optimized isocratic HPLC method consists of a C18 column, 0.02% triethylamine: methanol (15:85; % v/v), at a flow rate of 0.9 mL/min. The retention times were found to be 4.716 ± 0.082 min for MNE, 13.716 ± 0.188 min for MRN, and 2.241 ± 0.034 min for MMT using the developed method. The method was validated indetail and the quantification range found 20–180 µg/mL for both MNE and MRN and 1–9 µg/mL for MMT. In an alternative HPTLC method, toluene, ethyl acetate, and ethanol (10:3:2; v/v/v) were used as mobile phase on silica gel 60F254-coated aluminum sheets, with scanning at 240 nm. The MNE, MRN and MMT drugs spot eluted at Rf values of 0.65, 0.36, and 0.80, respectively. In-depth validation was performed and assessed statistically, including, specificity, precision, accuracy, reproducibility, and accuracy. The HPTLC method found linear in ranges of 1200–5200 ng/band for MNE and MRN, and 60–260 ng/band for MMT. Consequently, these developed chromatographic methods assessed according to green analytical principle and found sustainable. The developed methods can be effectively applied for the quantitative analysis of commercially available dosage forms.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 7-8","pages":"451 - 462"},"PeriodicalIF":1.2,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141061646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Validation and Application of a Dried Blood Spot Amoxicillin Assay 干血斑阿莫西林检测法的验证与应用
IF 1.2 4区 化学
Chromatographia Pub Date : 2024-05-15 DOI: 10.1007/s10337-024-04341-z
Madhu Page-Sharp, Okhee Yoo, Sam Salman, Timothy M. E. Davis, Brioni R. Moore, Laurens Manning, Kevin T. Batty
{"title":"Validation and Application of a Dried Blood Spot Amoxicillin Assay","authors":"Madhu Page-Sharp,&nbsp;Okhee Yoo,&nbsp;Sam Salman,&nbsp;Timothy M. E. Davis,&nbsp;Brioni R. Moore,&nbsp;Laurens Manning,&nbsp;Kevin T. Batty","doi":"10.1007/s10337-024-04341-z","DOIUrl":"10.1007/s10337-024-04341-z","url":null,"abstract":"<div><p>Dried blood spot (DBS) antibiotic assays can facilitate pharmacokinetic (PK) investigations in situations where venous blood sampling is logistically and/or ethically challenging. The aim of this study was to establish, validate and demonstrate the application of a DBS amoxicillin assay for PK studies in vulnerable populations. The matrix effect, process efficiency (84–104%) and recovery (85–110%) of the liquid chromatography–mass spectrometry (LC–MS/MS) assay for amoxicillin in DBS was determined at 1, 10 and 100 µg/mL, and three different haematocrits. Thermal stability studies of amoxicillin in DBS were performed and a bridging study comprising 26 paired plasma and DBS samples was conducted in four healthy individuals. The limits of detection and quantification were 0.02 and 0.05 µg/mL for plasma and DBS amoxicillin assays, respectively. Accuracy and interday precision of amoxicillin in DBS (0.1–100 µg/mL) were 88–103% and 4.5–9.2%, respectively. At room temperature (22 °C) and 4 °C, amoxicillin was stable in DBS for ≈4 and 26 h, respectively. There was no degradation of amoxicillin in DBS at −20 °C for &gt; 6 months. When comparing DBS and plasma collected from healthy volunteers, the slope of the Deming regression was 0.74. Amoxicillin CL/F estimates from DBS and plasma concentration data were 40.8 and 30.7 L/h/70 kg, respectively; V/F was 43.2 and 37.4 L/70 kg, respectively. In conclusion, amoxicillin can be reliably assayed from DBS in research studies but may have limited application in therapeutic drug monitoring. Due to poor stability at room temperature, amoxicillin DBS samples should be promptly dried and placed in frozen storage.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 7-8","pages":"493 - 501"},"PeriodicalIF":1.2,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10337-024-04341-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140974581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tyrosine-Phosphorylated Peptide Imprinted Particles Prepared by Reversible Addition–Fragmentation Chain Transfer Polymerization and “Epitope” Strategy: Selective Recognition of Phosphorylated Angiotensin II 通过可逆加成-碎片链转移聚合和 "表位 "策略制备的酪氨酸磷酸化肽印迹颗粒:选择性识别磷酸化血管紧张素 II
IF 1.2 4区 化学
Chromatographia Pub Date : 2024-05-14 DOI: 10.1007/s10337-024-04340-0
Yongjian Wang, Nurimangul Muntiza, Wenbin Zhang, Hongfeng Zhang, Qinran Li, Qiliang Deng
{"title":"Tyrosine-Phosphorylated Peptide Imprinted Particles Prepared by Reversible Addition–Fragmentation Chain Transfer Polymerization and “Epitope” Strategy: Selective Recognition of Phosphorylated Angiotensin II","authors":"Yongjian Wang,&nbsp;Nurimangul Muntiza,&nbsp;Wenbin Zhang,&nbsp;Hongfeng Zhang,&nbsp;Qinran Li,&nbsp;Qiliang Deng","doi":"10.1007/s10337-024-04340-0","DOIUrl":"10.1007/s10337-024-04340-0","url":null,"abstract":"<div><p>Phosphorylation is one of the most common post-translational modifications of proteins. Recognition of phosphorylated peptides with high selectivity is an important prerequisite for the structural identification of protein phosphorylation. By the application of molecular imprinting technology, a kind of tyrosine-phosphorylated peptide imprinted particles was prepared by the combination of reversible addition-fragmentation chain transfer (RAFT) polymerization and “epitope” strategy that applied in tyrosine-phosphorylated peptides recognition. Phenylphosphonic acid was used as the dummy template of the phosphorylated angiotensin II, which was one of the natural tyrosine-phosphorylated peptides. After the template modified by hydrogen bond with ureidopropyl group on the surface of silica, the surface imprinted particles with controlled and imprinted shell were synthesized by radical polymerization with RAFT strategy. The imprinted particles were obtained after the peptide removed and dithioester group destructed under alkaline condition. The binding capacity of phenylphosphonic acid reached 0.198 mg g<sup>−1</sup> with imprinting factor (IF) as 2.70, while the binding capacity of phosphorylated angiotensin II reached 0.792 mg g<sup>−1</sup> with IF as 1.96, which were obviously higher than with IF of that without RAFT strategy. Furthermore, phosphorylated angiotensin II could be selectively recognized by the imprinted particles even in presence of angiotensin II without phosphorylated. The performance of the phosphopeptide recognition remained 92% after five cycles of adsorption and desorption. All these results demonstrated that the tyrosine-phosphorylated imprinted particles prepared by combing RAFT polymerization and “epitope” strategy are promising to achieve the phosphopeptide recognition with higher recognition ability, selectivity and reusability.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 7-8","pages":"503 - 516"},"PeriodicalIF":1.2,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140932180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quantitative Estimation of 10 Known Impurities from Indacaterol Acetate, Glycopyrronium, and Mometasone Furoate Dry Powder Inhalation Product 醋酸茚达特罗、甘草酸铵和糠酸莫米松干粉吸入产品中 10 种已知杂质的定量估计
IF 1.2 4区 化学
Chromatographia Pub Date : 2024-05-11 DOI: 10.1007/s10337-024-04339-7
Shrikant V. Kulkarni, Pushpavati R. Zinjad, Shrinivas G. Bhope, Mitesh Nagar, Sharad P. Panchgalle, Vijaykumar S. More
{"title":"Quantitative Estimation of 10 Known Impurities from Indacaterol Acetate, Glycopyrronium, and Mometasone Furoate Dry Powder Inhalation Product","authors":"Shrikant V. Kulkarni,&nbsp;Pushpavati R. Zinjad,&nbsp;Shrinivas G. Bhope,&nbsp;Mitesh Nagar,&nbsp;Sharad P. Panchgalle,&nbsp;Vijaykumar S. More","doi":"10.1007/s10337-024-04339-7","DOIUrl":"10.1007/s10337-024-04339-7","url":null,"abstract":"<div><p>Indacaterol, glycopyrronium, and mometasone furoate triple combination inhalable fixed-dose medicines are effectively used to treat asthma and various chronic pulmonary disorders. The study aimed to develop and validate a simple single-run RP-HPLC impurity quantitation method. The chromatographic separation was accomplished using gradient elution mode of mobile phase A (potassium dihydrogen phosphate buffer pH 2.2) and mobile phase B (mixture of acetonitrile and methanol), with a flow rate of 0.8 mL/min using YMC Triart, C<sub>18</sub> (250 × 4.6 mm, 5 µm) HPLC column at 45 °C and the detection wavelength of 210 nm (for indacaterol, glycopyrronium and their impurities) and 248 nm (for mometasone furoate and its impurities). Water and methanol (20:80) were used as a diluent. Quantitation of 10 known and several unknown impurities was successfully performed with the determination of relative response factors for all the known impurities. The developed method was validated as per the ICH Q2(R1) guidelines. The stability indicating the nature of the method was proved by performing stress study on the sample and placebo. The linearity and range of the method were proved by calculating the r<sup>2</sup> values (&gt; 0.998). The overall precision was found to be within 1.82 − 7.76% RSD. The recovery for all the actives and known impurities were within 90 − 115% with 0.4 − 12% RSD. The sample solution was stable for 2 days at room temperature. The developed method can be successfully used for the impurity analysis of routine, stability, and commercial samples in a quality control laboratory of the pharmaceutical industry.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 7-8","pages":"479 - 491"},"PeriodicalIF":1.2,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140932406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Stable and Sensitive TLC-Spray for Thiols, Disulfides and Thioesters 用于硫醇、二硫化物和硫代酯的稳定而灵敏的 TLC 喷雾剂
IF 1.2 4区 化学
Chromatographia Pub Date : 2024-05-11 DOI: 10.1007/s10337-024-04336-w
Paul P. Van Veldhoven
{"title":"A Stable and Sensitive TLC-Spray for Thiols, Disulfides and Thioesters","authors":"Paul P. Van Veldhoven","doi":"10.1007/s10337-024-04336-w","DOIUrl":"10.1007/s10337-024-04336-w","url":null,"abstract":"<div><p>A sensitive and stable spray for thin layer chromatography of acyl-CoA and other thio-compounds has been evaluated. CoA, down to 1 nmol, is readily revealed and by pre- or post-treatment with hydroxylamine or tris(2-carboxyethyl)phosphine, thioesters or disulfides respectively can be stained as well.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 6","pages":"445 - 449"},"PeriodicalIF":1.2,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140932341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Filtration Analysis of Microparticles Using Paper-Based Microfluidics 利用纸基微流体技术进行微颗粒过滤分析
IF 1.2 4区 化学
Chromatographia Pub Date : 2024-05-10 DOI: 10.1007/s10337-024-04337-9
K. S. Bhuvaneshwari, Z. E. Jeroish, Abhishek Futane, Fahmi Samsuri, Vigneswaran Narayanamurthy
{"title":"Filtration Analysis of Microparticles Using Paper-Based Microfluidics","authors":"K. S. Bhuvaneshwari,&nbsp;Z. E. Jeroish,&nbsp;Abhishek Futane,&nbsp;Fahmi Samsuri,&nbsp;Vigneswaran Narayanamurthy","doi":"10.1007/s10337-024-04337-9","DOIUrl":"10.1007/s10337-024-04337-9","url":null,"abstract":"<div><p>A virus is a sub-microscopic infectious organism that causes diseases in humans, animals, and plants resulting in morbidity and may cause mortality. Proper diagnosis is necessary to initiate the treatment and pave the way to eradicate the viral infection. The current diagnostic kits for nucleic acid amplification assay, blood filtration, single-cell analysis are highly accurate, even though the procedure necessitates large sample volumes, complicated fabrication steps, time-consuming processes, and high costs. The filtration of viral samples from the blood is a tedious process. In this research, we have presented a home-based fabricated paper microfluidic chip to effectively filtrate viral particles from the sample to facilitate the nucleic acid amplification assay. The filtration analysis was exhibited for lateral and vertical flow paper chips fabricated via laser printing and polyethylene terephthalate (PET) encapsulation that circumvents the necessity of a traditional wax printer and hot plate. The results convey that the vertical flow paper chip with grade 4 inlet and outlet filters 98.57% of unnecessary particles from the sample. The paper-based microfluidic chip developed in this research is simple, easy to fabricate, and inexpensive to access in underdeveloped countries. The paper chip can pave the way for applications like lab-on-chip devices, POC assays, rapid nucleic acid amplification tests, cell cultures, and biomolecular research.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 7-8","pages":"517 - 527"},"PeriodicalIF":1.2,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140934746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Measurement of Serum Vitamins B2 and B6 in Patients with Hashimoto’s Thyroiditis by LC–MS/MS Method 用 LC-MS/MS 方法测定桥本氏甲状腺炎患者血清中的维生素 B2 和 B6
IF 1.2 4区 化学
Chromatographia Pub Date : 2024-05-04 DOI: 10.1007/s10337-024-04319-x
Aniceta Ada Mikulska-Sauermann, Marta Karaźniewicz-Łada, Dorota Filipowicz, Marek Ruchała, Franciszek Główka
{"title":"Measurement of Serum Vitamins B2 and B6 in Patients with Hashimoto’s Thyroiditis by LC–MS/MS Method","authors":"Aniceta Ada Mikulska-Sauermann,&nbsp;Marta Karaźniewicz-Łada,&nbsp;Dorota Filipowicz,&nbsp;Marek Ruchała,&nbsp;Franciszek Główka","doi":"10.1007/s10337-024-04319-x","DOIUrl":"10.1007/s10337-024-04319-x","url":null,"abstract":"<div><p>Vitamin B2 and vitamin B6 are cofactors and coenzymes in many biochemical reactions, and their sufficient serum concentrations may be crucial for the proper functioning of the thyroid gland. The aim of the study was to develop and validate a novel high-performance liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the determination of vitamin B2 and vitamin B6 in the serum of patients with Hashimoto’s thyroiditis (HT). Detection of the analytes was performed on a triple-quadrupole MS with multiple reaction monitoring using electrospray ionization. The mobile phase consisted of water and methanol, both containing 0.1% (v/v) formic acid. The analytes were isolated from serum by the one-step sample preparation of the trichloroacetic acid precipitation. Validation parameters, including linearity, accuracy, precision, limits of detection and quantification, and stability, were determined and fulfilled the criteria for analyzing compounds in biological fluids. The method was applied for the determination of the above vitamin concentrations in serum samples of 74 patients with HT and 33 healthy controls. The concentration of vitamin B2 was significantly lower in patients with HT compared to the control group (7.20 ± 7.86 ng/mL vs. 12.03 ± 9.62 ng/mL) and the concentration of vitamin B6 was also lower, but without a statistically significant difference (30.09 ± 19.16 ng/mL vs. 37.29 ± 23.02 ng/mL). We concluded that the vitamins concentration measurement may be helpful in the diagnosis of vitamin deficiencies and the supplementation strategy.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 6","pages":"433 - 443"},"PeriodicalIF":1.2,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10337-024-04319-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140889843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integration of Magnetic Dispersive Solid-Phase Micro-extraction with HPLC for Precise Detection of Tumor Markers 1-Methyladenosine and 1-Methylinosine in Urine Samples from Breast Cancer Patients 磁分散固相微萃取与高效液相色谱相结合,精确检测乳腺癌患者尿液样本中的肿瘤标志物 1-甲基腺苷和 1-甲基肌苷
IF 1.2 4区 化学
Chromatographia Pub Date : 2024-05-04 DOI: 10.1007/s10337-024-04334-y
Lei Hong, Huihui Mao, Xiaowei Huang, Xinyang Fu, Yanfang Lin
{"title":"Integration of Magnetic Dispersive Solid-Phase Micro-extraction with HPLC for Precise Detection of Tumor Markers 1-Methyladenosine and 1-Methylinosine in Urine Samples from Breast Cancer Patients","authors":"Lei Hong,&nbsp;Huihui Mao,&nbsp;Xiaowei Huang,&nbsp;Xinyang Fu,&nbsp;Yanfang Lin","doi":"10.1007/s10337-024-04334-y","DOIUrl":"10.1007/s10337-024-04334-y","url":null,"abstract":"<div><p>To analyze 1-methyladenosine (m1A) and 1-methylinosine (m1I) in urine samples obtained from patients with breast cancer and healthy people, we used magnetic dispersive solid-phase micro-extraction (MDSPME) combined with high-performance liquid chromatography. The structure and morphology of magnetic nanoparticles, Fe<sub>3</sub>O<sub>4</sub>@GO, were investigated by FT-IR, XRD, TEM, and TGA. m1A and m1I in urine samples were enriched by the solid phase micro-extraction technology of Fe<sub>3</sub>O<sub>4</sub>@GO. Several parameters affecting the extraction efficiency, including adsorption time, pH, ionic strength, mass of adsorbent, extraction time, eluent solvent, elution time, and elution temperature, were researched and optimized. Under the optimized conditions, the corresponding linearity range of the two analytes showed a good linearity (<i>r</i><sup>2</sup> &gt; 0.9998) and significant addition recoveries ranging from 88.50 to 109.38%, while the limit of detection was between 0.10 and 0.11 ng/mL. The enrichment factors of m1A and m1I were 108 and 134, respectively. The method was simple, sensitive, and reliable for the detection and purification with good detection limit, and it was suitable for the detection of m1A and m1I in urine samples. In addition, the analysis of modified nucleosides is of great value for the early diagnosis of patients with breast cancer.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 6","pages":"407 - 418"},"PeriodicalIF":1.2,"publicationDate":"2024-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140888437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Purification and Chromatographic Analyses of Cyclopentadienone Guaianolides from Artemisia leucodes Schrenk 从白花蒿中提纯环戊二烯酮胍内酯并进行色谱分析
IF 1.2 4区 化学
Chromatographia Pub Date : 2024-04-30 DOI: 10.1007/s10337-023-04285-w
S. M. Adekenov, Zh. R. Shaimerdenova, D. K. Nurkadirov, A. S. Adekenova, A. Berthod
{"title":"Purification and Chromatographic Analyses of Cyclopentadienone Guaianolides from Artemisia leucodes Schrenk","authors":"S. M. Adekenov,&nbsp;Zh. R. Shaimerdenova,&nbsp;D. K. Nurkadirov,&nbsp;A. S. Adekenova,&nbsp;A. Berthod","doi":"10.1007/s10337-023-04285-w","DOIUrl":"10.1007/s10337-023-04285-w","url":null,"abstract":"<div><p>Five cyclopentadienone sesquiterpene lactones: austricin, 5β(H)-austricin, achillin, grossmisin and leucomisin were obtained from the plant <i>Artemisia leucodes</i> Schrenk. The lactone purification procedure starting from the plant leaves is described. A quality control method was developed using thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). For these stereoisomeric cyclopentadienone guaianolides, the TLC and HPLC relationships “molecular structure—retention time” on the nature of the substituent in the molecular structure were studied. The chromatographic conditions were optimized and the influence of the structure of the molecule on their retention time was discussed comparing retention in both TLC and HPLC methods. The relationship between the structure of a particular cyclopentadienone guaianolide and its chromatographic retention time characterizes the sorption mechanism with the apolar stationary phase. It gives information on the compound polarity and hydrophobicity, information most relevant for these biologically active sesquiterpene γ-lactones, which are possible potent active ingredients of drugs.</p></div>","PeriodicalId":518,"journal":{"name":"Chromatographia","volume":"87 6","pages":"375 - 383"},"PeriodicalIF":1.2,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140830386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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