Enantioseparations of the Six Basic Drugs by MIL-100(Fe)-Modified Open-Tubular Columns in Capillary Electrochromatography

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Chromatographia Pub Date : 2024-12-19 DOI:10.1007/s10337-024-04382-4

Xiaozhen Shao, Guangfu Xu, Jiaquan Chen, Pandeng Miao, Ke Yang, Yingxiang Du, Tao Yu

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引用次数: 0

Abstract

Metal–organic frameworks (MOFs) have a wide range of applications in separation and analytical sciences due to their unique structures. MIL-100(Fe) was immobilized on the inner wall of the capillary column based on the immobilized cysteine (Cys)-triggered in situ growth (ICISG) strategy. By applying this column to capillary electrophoresis, a novel enantioselective separation system based on MIL-100(Fe) nanomaterial was established. By utilizing lactobionic acid (LA) as the chiral selector and optimizing the experimental conditions such as buffer pH, LA concentration and the methanol addition ratio, the CEC system demonstrated a significantly enhanced enantioseparation ability for six basic drugs. The modified capillary column was characterized by scanning electron microscope (SEM), energy-dispersive X-ray spectroscope (EDS) and X-ray diffractometer. The experimental results showed that MIL-100(Fe) was successfully grown on the inner wall of the capillary column. In the present study, the repeatability of the coated column was investigated and the relative standard deviations (RSDs) of the resolution and the migration time for intra-day, inter-day and inter-column were within 8%, proving the good repeatability of the coated column.

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MIL-100（Fe）修饰开管柱毛细管电色谱对6种基本药物的对映分离

金属有机骨架由于其独特的结构在分离和分析科学中有着广泛的应用。基于固定化半胱氨酸（Cys）触发原位生长（ICISG）策略，将MIL-100（Fe）固定在毛细管柱内壁上。将该柱应用于毛细管电泳，建立了MIL-100（Fe）纳米材料对映选择性分离体系。以乳酸（lactobionic acid， LA）为手性选择剂，优化缓冲液pH、乳酸浓度、甲醇添加比等实验条件，CEC体系对6种基本药物的对映体分离能力显著增强。采用扫描电镜（SEM）、能谱仪（EDS）和x射线衍射仪对改性后的毛细管柱进行了表征。实验结果表明，MIL-100（Fe）在毛细管柱内壁成功生长。本研究考察了包被柱的重复性，在日内、日间和柱间的分辨率和迁移时间的相对标准偏差（rsd）均在8%以内，证明包被柱具有良好的重复性。

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来源期刊

Chromatographia 化学-分析化学

CiteScore

3.40

自引率

5.90%

发文量

103

审稿时长

2.2 months

期刊介绍： Separation sciences, in all their various forms such as chromatography, field-flow fractionation, and electrophoresis, provide some of the most powerful techniques in analytical chemistry and are applied within a number of important application areas, including archaeology, biotechnology, clinical, environmental, food, medical, petroleum, pharmaceutical, polymer and biopolymer research. Beyond serving analytical purposes, separation techniques are also used for preparative and process-scale applications. The scope and power of separation sciences is significantly extended by combination with spectroscopic detection methods (e.g., laser-based approaches, nuclear-magnetic resonance, Raman, chemiluminescence) and particularly, mass spectrometry, to create hyphenated techniques. In addition to exciting new developments in chromatography, such as ultra high-pressure systems, multidimensional separations, and high-temperature approaches, there have also been great advances in hybrid methods combining chromatography and electro-based separations, especially on the micro- and nanoscale. Integrated biological procedures (e.g., enzymatic, immunological, receptor-based assays) can also be part of the overall analytical process.