The ISME Journal最新文献_第5页

Stronger compensatory thermal adaptation of soil microbial respiration with higher substrate availability 基质含量越高，土壤微生物呼吸的热补偿适应能力越强

The ISME Journal Pub Date : 2024-02-12 DOI: 10.1093/ismejo/wrae025

Lingrui Qu, Chao Wang, Stefano Manzoni, Marina Dacal, Fernando T Maestre, Edith Bai

{"title":"Stronger compensatory thermal adaptation of soil microbial respiration with higher substrate availability","authors":"Lingrui Qu, Chao Wang, Stefano Manzoni, Marina Dacal, Fernando T Maestre, Edith Bai","doi":"10.1093/ismejo/wrae025","DOIUrl":"https://doi.org/10.1093/ismejo/wrae025","url":null,"abstract":"Ongoing global warming is expected to augment soil respiration by increasing microbial activity, driving self-reinforcing feedback to climate change. However, the compensatory thermal adaptation of soil microorganisms and substrate depletion may weaken the effects of rising temperature on soil respiration. To test this hypothesis, we collected soils along a large-scale forest transect in eastern China spanning a natural temperature gradient, and incubated the soils at different temperatures with or without substrate addition. We combined the exponential thermal response function and a data-driven model to study the interaction effect of thermal adaptation and substrate availability on microbial respiration and compared our results to those from two additional continental and global independent datasets. Modelled results suggested that the effect of thermal adaptation on microbial respiration was greater in areas with higher mean annual temperatures, consistent with the compensatory response to warming. In addition, the effect of thermal adaptation on microbial respiration was greater under substrate addition than under substrate depletion, which was also true for the independent datasets reanalyzed using our approach. Our results indicate that thermal adaptation in warmer regions could exert a more pronounced negative impact on microbial respiration when substrate availability is abundant. These findings improve the body of knowledge on how substrate availability influences soil microbial community-temperature interactions, which could improve estimates of projected soil carbon losses to the atmosphere through respiration.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139728099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transglobal spread of an ecologically relevant sea urchin parasite 一种与生态相关的海胆寄生虫的跨全球传播

The ISME Journal Pub Date : 2024-02-08 DOI: 10.1093/ismejo/wrae024

Isabella T Ritchie, Brayan Vilanova-Cuevas, Ashley Altera, Kaileigh Cornfield, Ceri Evans, James S Evans, Maria Hopson-Fernandes, Christina A Kellogg, Elayne Looker, Oliver Taylor, Ian Hewson, Mya Breitbart

{"title":"Transglobal spread of an ecologically relevant sea urchin parasite","authors":"Isabella T Ritchie, Brayan Vilanova-Cuevas, Ashley Altera, Kaileigh Cornfield, Ceri Evans, James S Evans, Maria Hopson-Fernandes, Christina A Kellogg, Elayne Looker, Oliver Taylor, Ian Hewson, Mya Breitbart","doi":"10.1093/ismejo/wrae024","DOIUrl":"https://doi.org/10.1093/ismejo/wrae024","url":null,"abstract":"Mass mortality of the dominant coral reef herbivore Diadema antillarum in the Caribbean in the early 1980s led to a persistent phase shift from coral- to algal-dominated reefs. In 2022, a scuticociliate most closely related to Philaster apodigitiformis caused further mass mortality of D. antillarum across the Caribbean, leading to &gt;95% mortality at affected sites. Mortality was also reported in the related species Diadema setosum in the Mediterranean in 2022, where urchins experienced gross signs compatible with scuticociliatosis. However, the causative agent of the Mediterranean outbreak has not yet been determined. In April 2023, mass mortality of D. setosum occurred along the Sultanate of Oman’s coastline. Urchins displayed signs compatible with scuticociliatosis including abnormal behavior, drooping and loss of spines, followed by tissue necrosis and death. Here we report the detection of an 18S rRNA gene sequence in abnormal urchins from Muscat, Oman that is identical to the Philaster strain responsible for D. antillarum mass mortality in the Caribbean. We also show that scuticociliatosis signs can be elicited in D. setosum by experimental challenge with the cultivated Philaster strain associated with Caribbean scuticociliatosis. These results demonstrate the Philaster sp. associated with D. antillarum mass mortality has rapidly spread to geographically distant coral reefs, compelling global-scale awareness and monitoring for this devastating condition through field surveys, microscopy, and molecular microbiological approaches, and prompting investigation of long-range transmission mechanisms.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139716983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface-active antibiotic production as a multifunctional adaptation for postfire microorganisms 表面活性抗生素的生产是火灾后微生物的多功能适应性

The ISME Journal Pub Date : 2024-02-05 DOI: 10.1093/ismejo/wrae022

Mira D Liu, Yongle Du, Sara K Koupaei, Nicole R Kim, Monika S Fischer, Wenjun Zhang, Matthew F Traxler

{"title":"Surface-active antibiotic production as a multifunctional adaptation for postfire microorganisms","authors":"Mira D Liu, Yongle Du, Sara K Koupaei, Nicole R Kim, Monika S Fischer, Wenjun Zhang, Matthew F Traxler","doi":"10.1093/ismejo/wrae022","DOIUrl":"https://doi.org/10.1093/ismejo/wrae022","url":null,"abstract":"Wildfires affect soils in multiple ways, leading to numerous challenges for colonizing microorganisms. While it is thought that fire-adapted microorganisms lie at the forefront of postfire ecosystem recovery, the specific strategies that these organisms use to thrive in burned soils remain largely unknown. Through bioactivity screening of bacterial isolates from burned soils, we discovered that several Paraburkholderia spp. isolates produced a set of unusual rhamnolipid surfactants with a natural methyl ester modification. These rhamnolipid methyl esters (RLMEs) exhibited enhanced antimicrobial activity against other postfire microbial isolates, including pyrophilous Pyronema fungi and Amycolatopsis bacteria, compared to the typical rhamnolipids made by organisms such as Pseudomonas spp. RLMEs also showed enhanced surfactant properties and facilitated bacterial motility on agar surfaces. In vitro assays further demonstrated that RLMEs improved aqueous solubilization of polycyclic aromatic hydrocarbons, which are potential carbon sources found in char. Identification of the rhamnolipid biosynthesis genes in the postfire isolate, Paraburkholderia kirstenboschensis str. F3, led to the discovery of rhlM, whose gene product is responsible for the unique methylation of rhamnolipid substrates. RhlM is the first characterized bacterial representative of a large class of integral membrane methyltransferases that are widespread in bacteria. These results indicate multiple roles for RLMEs in the postfire lifestyle of Paraburkholderia isolates, including enhanced dispersal, solubilization of potential nutrients, and inhibition of competitors. Our findings shed new light on the chemical adaptations that bacteria employ to navigate, grow, and outcompete other soil community members in postfire environments.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139700852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commensal protist Tritrichomonas musculus exhibits a dynamic life cycle that induces extensive remodeling of the gut microbiota 共生原生动物麝香瘰疬单胞菌（Tritrichomonas musculus）的动态生命周期可诱导肠道微生物群的广泛重塑

The ISME Journal Pub Date : 2024-02-02 DOI: 10.1093/ismejo/wrae023

Ana Popovic, Eric Yixiao Cao, Joanna Han, Nirvana Nursimulu, Eliza V C Alves-Ferreira, Kyle Burrows, Andrea Kennard, Noor Alsmadi, Michael E Grigg, Arthur Mortha, John Parkinson

{"title":"Commensal protist Tritrichomonas musculus exhibits a dynamic life cycle that induces extensive remodeling of the gut microbiota","authors":"Ana Popovic, Eric Yixiao Cao, Joanna Han, Nirvana Nursimulu, Eliza V C Alves-Ferreira, Kyle Burrows, Andrea Kennard, Noor Alsmadi, Michael E Grigg, Arthur Mortha, John Parkinson","doi":"10.1093/ismejo/wrae023","DOIUrl":"https://doi.org/10.1093/ismejo/wrae023","url":null,"abstract":"Commensal protists and gut bacterial communities exhibit complex relationships, mediated at least in part through host immunity. To improve our understanding of this tripartite interplay, we investigated community and functional dynamics between the murine protist Tritrichomonas musculus (Tmu) and intestinal bacteria in healthy and B cell-deficient mice. We identified dramatic, protist-driven remodeling of resident microbiome growth and activities, in parallel with Tmu functional changes, which were accelerated in the absence of B cells. Metatranscriptomic data revealed nutrient-based competition between bacteria and the protist. Single cell transcriptomics identified distinct Tmu life stages, providing new evidence for trichomonad sexual replication and the formation of pseudocysts. Unique cell states were validated in situ through microscopy and flow cytometry. Our results reveal complex microbial dynamics during the establishment of a commensal protist in the gut, and provide valuable datasets to drive future mechanistic studies.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139700815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selection of photosynthetic traits by turbulent mixing governs formation of cyanobacterial blooms in shallow eutrophic lakes 湍流混合对光合特性的选择制约着浅层富营养化湖泊中蓝藻藻华的形成

The ISME Journal Pub Date : 2024-02-02 DOI: 10.1093/ismejo/wrae021

Huaming Wu, Xingqiang Wu, Lorenzo Rovelli, Andreas Lorke

{"title":"Selection of photosynthetic traits by turbulent mixing governs formation of cyanobacterial blooms in shallow eutrophic lakes","authors":"Huaming Wu, Xingqiang Wu, Lorenzo Rovelli, Andreas Lorke","doi":"10.1093/ismejo/wrae021","DOIUrl":"https://doi.org/10.1093/ismejo/wrae021","url":null,"abstract":"Prediction of the complex cyanobacteria-environment interactions is vital for understanding harmful bloom formation. Most previous studies on these interactions considered specific properties of cyanobacterial cells as representative for the entire population (e.g., growth rate, mortality, and photosynthetic capacity (Pmax)), and assumed that they remained spatiotemporally unchanged. Although, at the population level, the alteration of such traits can be driven by intraspecific competition, little is known about how traits and their plasticity change in response to environmental conditions and affect the bloom formation. Here we test the hypothesis that intraspecific variations in Pmax of cyanobacteria (Microcystis spp.) play an important role in its population dynamics. We coupled a one-dimensional hydrodynamic model with a trait-based phytoplankton model to simulate the effects of physical drivers (turbulence and turbidity) on the Pmax of Microcystis populations for a range of dynamic conditions typical for shallow eutrophic lakes. Our results revealed that turbulence acts as a directional selective driver for changes in Pmax. Depending on the intensity of daily-periodic turbulence, representing wind-driven mixing, a shift in population-averaged phenotypes occurred toward either low Pmax, allowing the population to capture additional light in the upper layers, or high Pmax, enhancing the efficiency of light utilization. Moreover, we observed that a high intraspecific diversity in Pmax accelerated the formation of surface scum by up to more than four times compared to a lower diversity. This study offers insights into mechanisms by which cyanobacteria populations respond to turbulence and underscores the significance of intraspecific variations in cyanobacterial bloom formation. Highlights","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139676879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotic interactions between benthic infauna and aerobic methanotrophs mediate methane fluxes from coastal sediments 底栖生物和好氧甲烷营养体之间的生物相互作用介导了沿海沉积物的甲烷通量

The ISME Journal Pub Date : 2024-02-01 DOI: 10.1093/ismejo/wrae013

Elias Broman, Markus Olsson, Adele Maciute, Daniel Donald, Christoph Humborg, Alf Norkko, Tom Jilbert, Stefano Bonaglia, Francisco J A Nascimento

{"title":"Biotic interactions between benthic infauna and aerobic methanotrophs mediate methane fluxes from coastal sediments","authors":"Elias Broman, Markus Olsson, Adele Maciute, Daniel Donald, Christoph Humborg, Alf Norkko, Tom Jilbert, Stefano Bonaglia, Francisco J A Nascimento","doi":"10.1093/ismejo/wrae013","DOIUrl":"https://doi.org/10.1093/ismejo/wrae013","url":null,"abstract":"Coastal ecosystems dominate oceanic methane (CH4) emissions. However, there is limited knowledge about how biotic interactions between infauna and aerobic methanotrophs (i.e. CH4 oxidizing bacteria) drive the spatial–temporal dynamics of these emissions. Here, we investigated the role of meio- and macrofauna in mediating CH4 sediment–water fluxes and aerobic methanotrophic activity that can oxidize significant portions of CH4. We show that macrofauna increases CH4 fluxes by enhancing vertical solute transport through bioturbation, but this effect is somewhat offset by high meiofauna abundance. The increase in CH4 flux reduces CH4 pore-water availability, resulting in lower abundance and activity of aerobic methanotrophs, an effect that counterbalances the potential stimulation of these bacteria by higher oxygen flux to the sediment via bioturbation. These findings indicate that a larger than previously thought portion of CH4 emissions from coastal ecosystems is due to faunal activity and multiple complex interactions with methanotrophs.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139660138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coral mucus as a reservoir of bacteriophages targeting Vibrio pathogens 珊瑚粘液是针对弧菌病原体的噬菌体的储存库

The ISME Journal Pub Date : 2024-02-01 DOI: 10.1093/ismejo/wrae017

Esther Rubio-Portillo, Sophia Robertson, Josefa Antón

{"title":"Coral mucus as a reservoir of bacteriophages targeting Vibrio pathogens","authors":"Esther Rubio-Portillo, Sophia Robertson, Josefa Antón","doi":"10.1093/ismejo/wrae017","DOIUrl":"https://doi.org/10.1093/ismejo/wrae017","url":null,"abstract":"The increasing trend in sea surface temperature promotes the spread of Vibrio species, which are known to cause diseases in a wide range of marine organisms. Among these pathogens, Vibrio mediterranei has emerged as a significant threat, leading to bleaching in the coral species Oculina patagonica. Bacteriophages, or phages, are viruses that infect bacteria, thereby regulating microbial communities and playing a crucial role in the coral’s defense against pathogens. However, our understanding of phages that infect V. mediterranei is limited. In this study, we identified two phage species capable of infecting V. mediterranei, utilizing a combination of cultivation and metagenomic approaches. These phages are low-abundance specialists within the coral mucus layer that exhibit rapid proliferation in the presence of their hosts, suggesting a potential role in coral defense. Additionally, one of these phages possesses a conserved domain of a leucine-rich repeat protein, similar to those harbored in the coral genome, that plays a key role in pathogen recognition, hinting at potential coral-phage coevolution. Furthermore, our research suggests that lytic Vibrio infections could trigger prophage induction, which may disseminate genetic elements, including virulence factors, in the coral mucus layer. Overall, our findings underscore the importance of historical coral-phage interactions as a form of coral immunity against invasive Vibrio pathogens.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139660092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interspecific interactions facilitate keystone species in a multispecies biofilm that promotes plant growth 在促进植物生长的多物种生物膜中，种间相互作用有利于关键物种的生长

The ISME Journal Pub Date : 2024-01-29 DOI: 10.1093/ismejo/wrae012

Nan Yang, Henriette L Røder, Wisnu Adi Wicaksono, Birgit Wassermann, Jakob Russel, Xuanji Li, Joseph Nesme, Gabriele Berg, Søren J Sørensen, Mette Burmølle

{"title":"Interspecific interactions facilitate keystone species in a multispecies biofilm that promotes plant growth","authors":"Nan Yang, Henriette L Røder, Wisnu Adi Wicaksono, Birgit Wassermann, Jakob Russel, Xuanji Li, Joseph Nesme, Gabriele Berg, Søren J Sørensen, Mette Burmølle","doi":"10.1093/ismejo/wrae012","DOIUrl":"https://doi.org/10.1093/ismejo/wrae012","url":null,"abstract":"Microorganisms colonizing plant roots co-exist in complex, spatially structured multispecies biofilm communities. However, little is known about microbial interactions and the underlying spatial organization within biofilm communities established on plant roots. Here, a well-established four-species biofilm model (Stenotrophomonas rhizophila, Paenibacillus amylolyticus, Microbacterium oxydans and Xanthomonas retroflexus, termed as SPMX) was applied to Arabidopsis roots to study the impact of multispecies biofilm on plant growth and the community spatial dynamics on the roots. SPMX co-culture notably promoted root development and plant biomass. Co-cultured SPMX increased root colonization and formed multispecies biofilms, structurally different from those formed by monocultures. By combining 16S rRNA gene amplicon sequencing and fluorescence in situ hybridization with confocal laser scanning microscopy (FISH-CLSM), we found that the composition and spatial organization of the four-species biofilm significantly changed over time. Monoculture P. amylolyticus colonized plant roots poorly, but its population and root colonization were highly enhanced when residing in the four-species biofilm. Exclusion of P. amylolyticus from the community reduced overall biofilm production and root colonization of the three species, resulting in the loss of the plant growth-promoting effects. Combined with spatial analysis, this led to identification of P. amylolyticus as a keystone species. Our findings highlight that weak root colonizers may benefit from mutualistic interactions in complex communities and hereby become important keystone species impacting community spatial organization and function. This work expands the knowledge on spatial organization uncovering interspecific interactions in multispecies biofilm communities on plant roots, beneficial for harnessing microbial mutualism promoting plant growth.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139660175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of carbon substrate competition among heterotrophic microorganisms. 异养微生物之间碳基质竞争的动态变化。

The ISME Journal Pub Date : 2024-01-29 DOI: 10.1093/ismejo/wrae018

Samuel M McNichol, Fernando Sanchez-Quete, Stephanie K Loeb, Andreas Teske, Sunita R Shah Walter, Nagissa Mahmoudi

{"title":"Dynamics of carbon substrate competition among heterotrophic microorganisms.","authors":"Samuel M McNichol, Fernando Sanchez-Quete, Stephanie K Loeb, Andreas Teske, Sunita R Shah Walter, Nagissa Mahmoudi","doi":"10.1093/ismejo/wrae018","DOIUrl":"https://doi.org/10.1093/ismejo/wrae018","url":null,"abstract":"Growing evidence suggests that interactions among heterotrophic microorganisms influence the efficiency and rate of organic matter turnover. These interactions are dynamic and shaped by the composition and availability of resources in their surrounding environment. Heterotrophic microorganisms inhabiting marine environments often encounter fluctuations in the quality and quantity of carbon inputs, ranging from simple sugars to large, complex compounds. Here, we experimentally tested how the chemical complexity of carbon substrates affects competition and growth dynamics between two heterotrophic marine isolates. We tracked cell density using species-specific PCR assays and measured rates of microbial CO2 production along with associated isotopic signatures (13C and 14C) to quantify the impact of these interactions on organic matter remineralization. The observed cell densities revealed substrate-driven interactions: one species exhibited a competitive advantage and quickly outgrew the other when incubated with a labile compound whereas both species seemed to coexist harmoniously in the presence of more complex organic matter. Rates of CO2 respiration revealed that co-incubation of these isolates enhanced organic matter turnover, sometimes by nearly twofold, compared to their incubation as mono-cultures. Isotopic signatures of respired CO2 indicated that co-incubation resulted in a greater remineralization of macromolecular organic matter. These results demonstrate that simple substrates promote competition whereas high substrate complexity reduces competitiveness and promotes the partitioning of degradative activities into distinct niches, facilitating coordinated utilization of the carbon pool. Taken together, this study yields new insight into how the quality of organic matter plays a pivotal role in determining microbial interactions within marine environments.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139577413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protozoan predation enhances stress resistance and antibiotic tolerance in Burkholderia cenocepacia by triggering the SOS response 原生动物捕食通过触发 SOS 反应增强伯克霍尔德氏原虫的抗压性和抗生素耐受性

The ISME Journal Pub Date : 2024-01-28 DOI: 10.1093/ismejo/wrae014

Álvaro Morón, Alaa E Tarhouchi, Iván Belinchón, Juan M Valenzuela, Patricia de Francisco, Ana Martín-González, Francisco Amaro

{"title":"Protozoan predation enhances stress resistance and antibiotic tolerance in Burkholderia cenocepacia by triggering the SOS response","authors":"Álvaro Morón, Alaa E Tarhouchi, Iván Belinchón, Juan M Valenzuela, Patricia de Francisco, Ana Martín-González, Francisco Amaro","doi":"10.1093/ismejo/wrae014","DOIUrl":"https://doi.org/10.1093/ismejo/wrae014","url":null,"abstract":"Bacterivorous protists are thought to serve as training grounds for bacterial pathogens by subjecting them to the same hostile conditions that they will encounter in the human host. Bacteria that survive intracellular digestion exhibit enhanced virulence and stress resistance after successful passage through protozoa but the underlying mechanisms are unknown. Here we show that the opportunistic pathogen Burkholderia cenocepacia survives phagocytosis by ciliates found in domestic and hospital sink drains, and viable bacteria are expelled packaged in respirable membrane vesicles with enhanced resistance to oxidative stress, desiccation and antibiotics, thereby contributing to pathogen dissemination in the environment. Reactive oxygen species generated within the protozoan phagosome promote the formation of persisters tolerant to ciprofloxacin by activating the bacterial SOS response. In addition, we show that genes encoding antioxidant enzymes are upregulated during passage through ciliates increasing bacterial resistance to oxidative radicals. We prove that suppression of the SOS response impairs bacterial intracellular survival and persister formation within protists. This study highlights the significance of protozoan food vacuoles as niches that foster bacterial adaptation in natural and built environments and suggests that persister switch within phagosomes may be a widespread phenomenon in bacteria surviving intracellular digestion.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139577385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0