The ISME Journal最新文献_第4页

Mapping the loss of flagellar motility across the tree of life 勾勒出鞭毛运动能力丧失的整个生命之树

The ISME Journal Pub Date : 2025-06-13 DOI: 10.1093/ismejo/wraf111

Jamiema Sara Philip, Sehhaj Grewal, Jacob Scadden, Caroline Puente-Lelievre, Nicholas J Matzke, Luke McNally, Matthew A B Baker

{"title":"Mapping the loss of flagellar motility across the tree of life","authors":"Jamiema Sara Philip, Sehhaj Grewal, Jacob Scadden, Caroline Puente-Lelievre, Nicholas J Matzke, Luke McNally, Matthew A B Baker","doi":"10.1093/ismejo/wraf111","DOIUrl":"https://doi.org/10.1093/ismejo/wraf111","url":null,"abstract":"Bacterial swimming is mostly powered by the bacterial flagellar motor and the number of proteins involved in the flagellar motor can vary. Quantifying the proteins present in flagellar motors from a range of species delivers insight into how motility has changed throughout history and provides a platform for estimating from its genome whether a species is likely to be motile. We conducted sequence and structural homology searches for 54 flagellar pathway proteins across 11 365 bacterial genomes and developed a classifier with up to 95% accuracy that could predict whether a strain was motile or not. We then mapped the evolution of flagellar motility across the GTDB bacterial tree of life. We confirmed that the last common bacterial ancestor had flagellar motility and that the rate of loss of this motility was four-fold higher than the rate of gain. We showed that the presence of filament protein homologues was highly phylogenetically correlated with motility and that all species classified as motile contained at least one filament homologue. We calculated the rate of gain and loss for each flagellar protein and that the filament protein FliC was highly correlated with motility across the tree of life. We then measured the correlation of each flagellar motor protein with FliC and showed that the filament, rotor, and rod and hook proteins were all highly correlated with FliC, and thus with motility. We calculated the differential rates of gain and loss for each flagellar protein and quantified which genomes encoded for partial sets of flagellar proteins, indicating potential pathways by which motility could be lost. Overall, we show that filament, rod and hook and rotor proteins are conserved when flagellar motility is preserved and that the presence or absence of a FliC homologue is a good, simple predictor of whether or not a species has flagellar motility.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uptake and leakage rates differentially shape community arrangement and composition of microbial consortia 吸收率和渗漏率不同地决定了群落的排列和微生物群落的组成

The ISME Journal Pub Date : 2025-06-13 DOI: 10.1093/ismejo/wraf122

Estelle Pignon, Gábor Holló, Théodora Steiner, Simon van Vliet, Yolanda Schaerli

{"title":"Uptake and leakage rates differentially shape community arrangement and composition of microbial consortia","authors":"Estelle Pignon, Gábor Holló, Théodora Steiner, Simon van Vliet, Yolanda Schaerli","doi":"10.1093/ismejo/wraf122","DOIUrl":"https://doi.org/10.1093/ismejo/wraf122","url":null,"abstract":"Bacteria often grow as communities in intricate spatial arrangements on surfaces and interact with each other through the local exchange of diffusible molecules. Yet, our understanding of how these metabolite exchanges shape the properties of the communities remains limited. Here, we study synthetic communities of Escherichia coli amino acid auxotrophs interacting through the obligate exchange of amino acids. We genetically engineer these strains to alter their amino acid leakage and uptake abilities. We then characterise the spatial arrangement and composition of the communities when grown on a surface and compare these to qualitative predictions of a previously developed analytical model for cells growing in two dimensions. Our experiments provide empirical validation of the model’s central hypothesis: higher uptake rates reduce sector widths and promote mixing, while increased leakage rate of an amino acid increases the frequency of the strain benefiting from this amino acid. We thus extend the relevance of this simplified model to more complex, three-dimensional systems, while also identifying its limitations. Our findings provide critical insights into microbial community dynamics and establish a predictive framework for designing and engineering microbial consortia.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemosynthesis enhances net primary production and nutrient cycling in a hypersaline microbial mat 化学合成提高了高盐微生物垫的净初级生产和养分循环

The ISME Journal Pub Date : 2025-06-09 DOI: 10.1093/ismejo/wraf117

Francesco Ricci, Pok Man Leung, Tess Hutchinson, Thanh Nguyen-Dinh, Alexander H Frank, Ashleigh V S Hood, Vinícius W Salazar, Vera Eate, Wei Wen Wong, Perran L M Cook, Chris Greening, Harry McClelland

{"title":"Chemosynthesis enhances net primary production and nutrient cycling in a hypersaline microbial mat","authors":"Francesco Ricci, Pok Man Leung, Tess Hutchinson, Thanh Nguyen-Dinh, Alexander H Frank, Ashleigh V S Hood, Vinícius W Salazar, Vera Eate, Wei Wen Wong, Perran L M Cook, Chris Greening, Harry McClelland","doi":"10.1093/ismejo/wraf117","DOIUrl":"https://doi.org/10.1093/ismejo/wraf117","url":null,"abstract":"Photosynthetic microbial mats are macroscopic microbial ecosystems consisting of a wide array of functional groups and microenvironments arranged along variable redox gradients. Light energy ultimately drives primary production and a cascade of daisy-chained metabolisms. Heterotrophic members of these communities remineralise organic material, decreasing net primary production, and returning nutrients to the aqueous phase. However, reduced inorganic and one-carbon substrates such as trace gases and those released as metabolic byproducts in deeper anoxic regions of the mat, could theoretically also fuel carbon fixation, mitigating carbon loss from heterotrophy and enhancing net primary production. Here, we investigated the intricate metabolic synergies that sustain community nutrient webs in a biomineralising microbial mat from a hypersaline lake. We recovered 331 genomes spanning 40 bacterial and archaeal phyla that influence the biogeochemistry of these ecosystems. Phototrophy is a major metabolic potential found in 17% of the genomes, but over 50% encode enzymes to harness energy from inorganic substrates and 12% co-encode chemosynthetic carbon fixation pathways that use sulfide and hydrogen as electron donors. We experimentally demonstrated that the microbial community oxidises ferrous iron, ammonia, sulfide, and reduced trace gas substrates aerobically and anaerobically. Furthermore, carbon isotope assays revealed that diverse chemosynthetic pathways contribute significantly to carbon fixation and organic matter production alongside photosynthesis. Chemosynthesis in microbial mats results from a complex suite of spatially organised metabolic interactions and continuous nutrient cycling, which decouples carbon fixation from the diurnal cycle, and enhances the net primary production of these highly efficient ecosystems.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144237709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Saccharibacteria deploy two distinct type IV pili, driving episymbiosis, host competition, and twitching motility 糖菌部署两种不同的IV型菌毛，驱动附合、宿主竞争和抽搐运动

The ISME Journal Pub Date : 2025-06-07 DOI: 10.1093/ismejo/wraf119

Alex S Grossman, Lei Lei, Jack M Botting, Jett Liu, Nusrat Nahar, Jun Liu, Jeffrey S McLean, Xuesong He, Batbileg Bor

{"title":"Saccharibacteria deploy two distinct type IV pili, driving episymbiosis, host competition, and twitching motility","authors":"Alex S Grossman, Lei Lei, Jack M Botting, Jett Liu, Nusrat Nahar, Jun Liu, Jeffrey S McLean, Xuesong He, Batbileg Bor","doi":"10.1093/ismejo/wraf119","DOIUrl":"https://doi.org/10.1093/ismejo/wraf119","url":null,"abstract":"All cultivated Patescibacteria, also known as the candidate phyla radiation, are obligate episymbionts residing on other microbes. Despite being ubiquitous in many diverse environments, including mammalian microbiomes, molecular mechanisms of host identification and binding amongst ultrasmall bacterial episymbionts remain largely unknown. Type 4 pili are well conserved in this group and could potentially facilitate these symbiotic interactions. To test this hypothesis, we genetically targeted pili genes in Saccharibacteria Nanosynbacter lyticus strain TM7x to assess their essentiality and roles in symbiosis. Our results revealed that Nanosynbacter lyticus assembles two distinct type 4 pili: a non-essential thin pilus that has the smallest diameter of any type 4 pili and contributes to host-binding and episymbiont growth; and an essential thick pilus involved in twitching motility. To understand the role of these pili in vivo we developed Saccharibacteria competition assays and species specific Fluorescence in situ hybridization probes. Competition between different Saccharibacteria within mock communities demonstrated consistent competitive outcomes that were not driven by priority effects but were dependent on the thin pilus. Collectively our findings demonstrate that Saccharibacteria encode unique extracellular pili that enable their underexplored episymbiotic lifestyle and competitive fitness within a community.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144237708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro biofilm formation by a beneficial bacterium partially predicts in planta protection against rhizosphere pathogens 有益菌的体外生物膜形成部分预测了植物对根际病原体的保护

The ISME Journal Pub Date : 2025-06-02 DOI: 10.1093/ismejo/wraf114

Yang Liu, Alexandra D Gates, Zhexian Liu, Quinn Duque, Sierra S Schmidt, Melissa Y Chen, Corri D Hamilton, George A O’Toole, Cara H Haney

{"title":"In vitro biofilm formation by a beneficial bacterium partially predicts in planta protection against rhizosphere pathogens","authors":"Yang Liu, Alexandra D Gates, Zhexian Liu, Quinn Duque, Sierra S Schmidt, Melissa Y Chen, Corri D Hamilton, George A O’Toole, Cara H Haney","doi":"10.1093/ismejo/wraf114","DOIUrl":"https://doi.org/10.1093/ismejo/wraf114","url":null,"abstract":"Plant roots form associations with beneficial and pathogenic soil microorganisms. Although members of the rhizosphere microbiome can protect against pathogens, the mechanisms are poorly understood. We hypothesized that the ability to form a biofilm on the root surface is necessary for the exclusion of pathogens; however, it is not known if the same biofilm formation components required in vitro are necessary in vivo. Pseudomonas brassicacearum WCS365 is a beneficial strain that is phylogenetically closely related to an opportunistic pathogen Pseudomonas sp. N2C3 and confers protection against N2C3 in the rhizosphere. We used this plant-mutualist-pathogen model to screen collections of P. brassicacearum WCS365 increased attachment mutants (iam) and surface attachment defective (sad) transposon insertion mutants that form increased or decreased biofilm on abiotic surfaces, respectively. We found that whereas the P. brassicacearum WCS365 mutants had altered biofilm formation in vitro, only a subset of these mutants lost protection against N2C3. Non-protective mutants those involved in large adhesion protein (LapA) biosynthesis, flagellar synthesis and function, and O-antigen biosynthesis. We found that the inability of P. brassicacearum WCS365 mutants to grow in planta, and the inability to suppress pathogen growth, both partially contributed to loss of plant protection. We did not find a correlation between the extent of biofilm formed in vitro and pathogen protection in planta indicating that biofilm formation on abiotic surfaces may not fully predict pathogen exclusion in planta. Collectively, our work provides insights into mechanisms of biofilm formation and host colonization that shape the outcomes of host-microbe-pathogen interactions.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144193349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electroactive ecosystem insights from corrosion microbiomes inform gut microbiome modulation 从腐蚀微生物组电活性生态系统的见解告知肠道微生物组调节

The ISME Journal Pub Date : 2025-05-31 DOI: 10.1093/ismejo/wraf112

Liam M Jones, Sahar El Aidy

引用次数: 0

Integration of metatranscriptomics data improves the predictive capacity of microbial community metabolic models 整合元转录组学数据提高了微生物群落代谢模型的预测能力

The ISME Journal Pub Date : 2025-05-31 DOI: 10.1093/ismejo/wraf109

Yunli Eric Hsieh, Kshitij Tandon, Heroen Verbruggen, Zoran Nikoloski

{"title":"Integration of metatranscriptomics data improves the predictive capacity of microbial community metabolic models","authors":"Yunli Eric Hsieh, Kshitij Tandon, Heroen Verbruggen, Zoran Nikoloski","doi":"10.1093/ismejo/wraf109","DOIUrl":"https://doi.org/10.1093/ismejo/wraf109","url":null,"abstract":"Microbial consortia play pivotal roles in nutrient cycling across diverse ecosystems, where the functionality and composition of microbial communities are shaped by metabolic interactions. Despite the critical importance of understanding these interactions, accurately mapping and manipulating microbial interaction networks to achieve specific outcomes remains challenging. Genome-scale metabolic models (GEMs) offer significant promise for predicting microbial metabolic functions from genomic data; however, traditional community GEMs typically rely on species abundance information, which may limit their predictive accuracy due to the absence of condition-specific gene expression or protein abundance data. Here, we introduce the Integration of Metatranscriptomes Into Community GEMs (IMIC) approach, which utilizes metatranscriptomic data to construct context-specific community models for predicting individual growth rates and metabolic interactions. By incorporating metatranscriptomic profiles, which reflect both gene expression activity and partially encode abundance information, IMIC could predict condition-specific flux distributions that enable the investigation of metabolite interactions among community members. Our results show that growth rates predicted by IMIC correlate strongly with relative as well as absolute abundance of species and offer a streamlined, automated procedure for estimating the single intrinsic parameter. Specifically, IMIC results in improved predictions of measured metabolite concentration changes compared with other approaches in our case study. We further demonstrate that this improvement is driven by the network-wide adjustment of flux bounds based on gene expression profiles. In conclusion, IMIC approach enables the accurate prediction of individual growth rates and improves the model performance of predicting metabolite interactions, facilitating a deeper understanding of metabolic interdependencies within microbial communities.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen isotope fractionation during anaerobic ammonium oxidation by the marine representative Candidatus Scalindua sp. 海洋代表Candidatus Scalindua sp.厌氧氨氧化过程中的氧同位素分馏。

The ISME Journal Pub Date : 2025-05-31 DOI: 10.1093/ismejo/wraf115

Kanae Kobayashi, Kazuya Nishina, Keitaro Fukushima, Yuji Onishi, Akiko Makabe, Mamoru Oshiki, Keisuke Koba, Satoshi Okabe

{"title":"Oxygen isotope fractionation during anaerobic ammonium oxidation by the marine representative Candidatus Scalindua sp.","authors":"Kanae Kobayashi, Kazuya Nishina, Keitaro Fukushima, Yuji Onishi, Akiko Makabe, Mamoru Oshiki, Keisuke Koba, Satoshi Okabe","doi":"10.1093/ismejo/wraf115","DOIUrl":"https://doi.org/10.1093/ismejo/wraf115","url":null,"abstract":"Analyzing the nitrogen (15ε) and oxygen (18ε) isotope effects of anaerobic ammonium oxidation (anammox) is essential for accurately assessing its potential contribution to fixed-N losses in the ocean, yet the 18ε of anammox remains unexplored. Here, we determined the previously unexplored 18ε of anammox using a highly enriched culture of the marine anammox species “Ca. Scalindua sp”. Because Scalindua significantly accelerated oxygen isotope exchange between NO2- and H2O, we introduced a new rate constant for anammox-mediated oxygen isotope exchange (keq, AMX = 8.44 ~ 13.56 ×10-2 h-1), which is substantially faster than abiotic oxygen isotope exchange (keq, abio = 1.13 ×10-2 h-1), into a numerical model to estimate the 18ε during anammox. Based on our experimental results, we successfully determined the 18ε associated with: (1) conversion of NO2- to N2 (18εNO2-→N2 = 10.6 ~ 16.1‰), (2) NO2- oxidation to NO3- (18εNO2-→NO3- = -2.9 ~ -11.0‰, inverse fractionation), (3) incorporation of oxygen from water during NO2- oxidation to NO3- (18εH2O = 16.4 ~ 19.2‰). Our study underscores the possibility that unique anammox oxygen isotope signals may be masked due to substantial anammox-mediated oxygen isotope exchange between NO2- and H2O. Therefore, careful consideration is required when utilizing δ18ONO3- and δ18ONO2- as geochemical markers to assess the potential contribution of anammox to fixed-N losses in the ocean.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144193350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dehalogenating Desulfoluna spp. are Ubiquitous in Host-Specific Sponge Microbiomes of the Great Barrier Reef 在大堡礁的宿主特异性海绵微生物群中，脱卤的Desulfoluna spp普遍存在

The ISME Journal Pub Date : 2025-05-31 DOI: 10.1093/ismejo/wraf113

Lauren A Hall, Katherine D Scott, Nicole Webster, Lee J Kerkhof, Max M Häggblom

{"title":"Dehalogenating Desulfoluna spp. are Ubiquitous in Host-Specific Sponge Microbiomes of the Great Barrier Reef","authors":"Lauren A Hall, Katherine D Scott, Nicole Webster, Lee J Kerkhof, Max M Häggblom","doi":"10.1093/ismejo/wraf113","DOIUrl":"https://doi.org/10.1093/ismejo/wraf113","url":null,"abstract":"Marine sponge holobionts are important contributors to numerous biogeochemical cycles, including the natural organohalogen cycle. Sponges produce diverse brominated secondary metabolites, which select for a population of anaerobic debrominating bacteria within the sponge body. Sponge microbiomes can be host-specific, but the selection and host-specificity of debrominating bacteria are unknown currently. In this study, we used nanopore long-read sequencing of nearly full-length ribosomal RNA operons to evaluate host-specificity of the Great Barrier Reef sponge microbiomes at the strain level and to determine if host specificity extends to sponge-associated dehalogenating bacteria. Reductive debromination activity was observed in anaerobic enrichment cultures established from all Great Barrier Reef sponges. Even though other bacterial symbionts of interest, including Nitrospira spp. and Ca. Synechococcus spp. demonstrated strong host-specificity, Desulfoluna spp., a key sponge-associated dehalogenating bacterium showed no evidence of host-specificity. This suggests different modes of transmission and/or retention of different members of the sponge microbiome residing within the same host species. These findings expand our understanding of how sponge microbiomes are assembled and the relationship between the host and individual bacterial strains.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"134 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144188901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemotaxis, growth, and inter-species interactions shape early bacterial community assembly 趋化性、生长和种间相互作用形成了早期细菌群落的集合

The ISME Journal Pub Date : 2025-05-27 DOI: 10.1093/ismejo/wraf101

Estelle E Clerc, Jeremy E Schreier, Jonasz Słomka, Christa B Smith, He Fu, Justin R Seymour, Jean-Baptiste Raina, Mary Ann Moran, Roman Stocker

{"title":"Chemotaxis, growth, and inter-species interactions shape early bacterial community assembly","authors":"Estelle E Clerc, Jeremy E Schreier, Jonasz Słomka, Christa B Smith, He Fu, Justin R Seymour, Jean-Baptiste Raina, Mary Ann Moran, Roman Stocker","doi":"10.1093/ismejo/wraf101","DOIUrl":"https://doi.org/10.1093/ismejo/wraf101","url":null,"abstract":"From the perspective of a marine copiotrophic bacterium, the surface ocean is a mosaic of exploitable hotspots of organic matter released from living and senescing phytoplankton. Bacterial success in exploiting this patchy microscale environment relies on sensing and swimming towards the hotspots, and upon arrival, growing on available substrates. However, the combined effect of chemotaxis and growth rate on bacterial community assembly has never been quantified. Here, we characterized chemotaxis and growth rate responses of seven representative copiotrophic marine bacteria to phytoplankton exometabolites, both for single species and for pairs of species. We compared these results to prediction of a mathematical null model of hotspot community assembly. Our results revealed that the bacterial strains exhibit diverse responses to phytoplankton metabolites, which can act as either signals, substrates, or both. Interactions between bacterial pairs resulted in chemotactic responses or growth rates different from model predictions in all of the 12 pairs tested (92% differed in chemotaxis, 33% in growth). These community dynamics indicate that inter-species interaction is another factor shaping early colonization of metabolite hotspots, with beneficial, detrimental, and neutral associations observed between bacterial species. Such complex ecological interactions impact chemotactic behaviors and growth rates of marine bacteria on resource hotspots, affecting their community compositions and associated functions relevant to the cycling of key elements including carbon.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144153306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0