The ISME Journal最新文献_第4页

Predator-prey dynamics of Vibrio cholerae on chitin suggest an alternative mode of biofilm formation in marine snow conditions 几丁质上霍乱弧菌的捕食-猎物动力学提示了海洋雪条件下生物膜形成的另一种模式

The ISME Journal Pub Date : 2026-03-30 DOI: 10.1093/ismejo/wrag072

Jacob D Holt, Katherine A Miller, Olivia F Hunter, Emily Zhang, Alexander J Hinbest, Emma Gerace, Rich Olson, Daniel E Kadouri, Carey D Nadell

{"title":"Predator-prey dynamics of Vibrio cholerae on chitin suggest an alternative mode of biofilm formation in marine snow conditions","authors":"Jacob D Holt, Katherine A Miller, Olivia F Hunter, Emily Zhang, Alexander J Hinbest, Emma Gerace, Rich Olson, Daniel E Kadouri, Carey D Nadell","doi":"10.1093/ismejo/wrag072","DOIUrl":"https://doi.org/10.1093/ismejo/wrag072","url":null,"abstract":"Vibrio cholerae is a ubiquitous marine bacterium that solubilizes and consumes chitin in the marine water column. In both the marine environment and the intestinal track, V. cholerae forms biofilms: how do the diverse surfaces that V. cholerae encounters influence its biofilm formation and, in turn, shape its ecological interactions with other microbes? Here, we use the interaction between the predator Bdellovibrio bacteriovorus and V. cholerae as a model to explore how the environmental chitin substrate alters V. cholerae biofilm formation and predator-prey dynamics. We find that glass-bound biofilm growth provides strong protection for V. cholerae against predation while also allowing a population of predatory B. bacteriovorus to remain in place among prey cells. In contrast, chitin-bound biofilm structure offers less protection against B. bacteriovorus predation and does not maintain as stable a population of B. bacteriovorus. Using percolation and population dynamics models, we predict that these changes in predator-prey dynamics can be explained largely by alterations in V. cholerae biofilm architecture between the two conditions, which changes the fraction of prey available to B. bacteriovorus. Using targeted biofilm matrix gene deletions, we confirm this prediction by recapitulating key features of the chitin predator-prey interactions on glass surfaces. Following on this observation, we show that V. cholerae biofilms grown on chitin produce much less of the canonical biofilm matrix components and instead rely on other extracellular structures. Overall, our experiments detail how growth substrate can alter biofilm matrix composition and how these changes in biofilm architecture impact higher-order ecological interactions.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"418 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147536466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fecal microbiota transplantation and Akkermansia muciniphila restore neurodevelopment and behavior via the gut-brain axis in autism-like zebrafish 粪便微生物群移植和嗜粘阿克曼氏菌通过肠-脑轴恢复自闭症样斑马鱼的神经发育和行为

The ISME Journal Pub Date : 2026-03-28 DOI: 10.1093/ismejo/wrag074

Pan-Pan Jia, Yan Li, Hao-Yu Yang, Yuan Ding, Feng-Yi Guo, Ming-Fei Wu, Jin-Qiu Jia, De-Sheng Pei

{"title":"Fecal microbiota transplantation and Akkermansia muciniphila restore neurodevelopment and behavior via the gut-brain axis in autism-like zebrafish","authors":"Pan-Pan Jia, Yan Li, Hao-Yu Yang, Yuan Ding, Feng-Yi Guo, Ming-Fei Wu, Jin-Qiu Jia, De-Sheng Pei","doi":"10.1093/ismejo/wrag074","DOIUrl":"https://doi.org/10.1093/ismejo/wrag074","url":null,"abstract":"Effective therapies for Autism Spectrum Disorder (ASD) are currently limited, and the functional connections between gut microbiota and brain development are not fully elucidated. Using the Katnal2 mutant zebrafish as an ASD-like model, we evaluated whether fecal microbiota transplantation (FMT) from wild-type donors or supplementation with the probiotic Akkermansia muciniphila (A. muciniphila) could ameliorate neurodevelopmental deficits. Assessments included developmental phenotypes, behavior, microbial profiling, neurotransmitter-related gene expression, and short-chain fatty acid (SCFA) signaling in conventionally reared (CR) and germ-free (GF) fish. FMT from wild-type donors and A. muciniphila supplementation significantly improved hatching rates, growth parameters, heart rate, and locomotor activity in Katnal2 mutants, whereas microbiota from Katnal2 mutants induced analogous deficits in wild-type recipients. A. muciniphila successfully colonized the gut, reshaped microbial communities, and reduced anxiety-like behaviors. Mechanistically, A. muciniphila upregulates genes involved in dopamine (th), serotonin (tph1a), and gamma-aminobutyric acid (GABA) synthesis, downregulates the serotonin receptor htr3a, and enhances expression of the SCFA receptor ffar2, independently of total SCFA levels. Correlation analyses linked key developmental, behavioral, and transcriptional changes to altered microbial genera in a sample-specific manner, highlighting compositionally driven neuromodulatory effects of genetic and probiotic interventions. Thus, microbiota-targeted intervention with A. muciniphila rescues neurodevelopmental impairments in ASD models by remodeling the gut-brain axis, supporting its translational potential.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147536467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid resistance evolution against phage cocktails 对噬菌体鸡尾酒的快速抗性进化

The ISME Journal Pub Date : 2026-03-27 DOI: 10.1093/ismejo/wrag067

Baltus A van der Steen, Matti Gralka, Yuval Mulla

引用次数: 0

Global terrestrial distribution of N2O-reducing Acidobacteriota members 还原n2o酸杆菌群成员的全球陆地分布

The ISME Journal Pub Date : 2026-03-27 DOI: 10.1093/ismejo/wrag073

Kazumori Mise, Sawa Wasai-Hara, Hideomi Itoh

{"title":"Global terrestrial distribution of N2O-reducing Acidobacteriota members","authors":"Kazumori Mise, Sawa Wasai-Hara, Hideomi Itoh","doi":"10.1093/ismejo/wrag073","DOIUrl":"https://doi.org/10.1093/ismejo/wrag073","url":null,"abstract":"Nitrous oxide (N2O) is a potent greenhouse gas, and soil is its largest terrestrial source. Microbial N2O reductase (NosZ) is the only known enzyme capable of reducing N2O to N2, making nosZ-harboring prokaryotes important sinks in terrestrial ecosystems. Despite being among the most abundant and ubiquitous bacterial phyla in soil, the potential role of Acidobacteriota in N2O reduction remains largely unexplored. In this study, we addressed this gap using genomic, metagenomic, and physiological analyses. We first analyzed 199,602 prokaryotic genomes, including genomes from both isolated strains and metagenome-assembled genomes. We found that 491 Acidobacteriota genomes harbored nosZ, predominantly the Sec-dependent NosZ gene (nosZII). Global metagenomic analysis of 321 soil samples revealed that Acidobacteriota nosZII is one of the most abundant groups of nosZ and distributed across different continents. Among Acidobacteriota, nosZII from the class Vicinamibacteria was the most prevalent in the soils. Finally, we provide the physiological evidence of N2O-reducing activity in Acidobacteriota by demonstrating that the Vicinamibacteria type strain, Luteitalea pratensis KCTC52215T, can reduce N2O. Taken together, these findings highlight the previously overlooked potential role of Acidobacteriota as a global N2O sink and underscore the need to include them in future studies on soil N2O dynamics.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"229 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147524342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulfoquinovose degradation by cow rumen microbiota 牛瘤胃微生物群对磺胺喹诺糖的降解

The ISME Journal Pub Date : 2026-03-27 DOI: 10.1093/ismejo/wrag069

Julia Krasenbrink, Song-Can Chen, Tomohisa Sebastian Tanabe, Hüseyin Sarikeçe, Pleun Meurs, Sabrina Borusak, Rahul Samrat, Guoqing Guan, Clara Priemer, Jay Osvatic, Joana Séneca, Bela Hausmann, Daan R Speth, Evelyne Selberherr, Wolfgang Wanek, David Schleheck, Marc Mussmann, Alexander Loy

{"title":"Sulfoquinovose degradation by cow rumen microbiota","authors":"Julia Krasenbrink, Song-Can Chen, Tomohisa Sebastian Tanabe, Hüseyin Sarikeçe, Pleun Meurs, Sabrina Borusak, Rahul Samrat, Guoqing Guan, Clara Priemer, Jay Osvatic, Joana Séneca, Bela Hausmann, Daan R Speth, Evelyne Selberherr, Wolfgang Wanek, David Schleheck, Marc Mussmann, Alexander Loy","doi":"10.1093/ismejo/wrag069","DOIUrl":"https://doi.org/10.1093/ismejo/wrag069","url":null,"abstract":"Sulfoquinovose, a sulfonated sugar derived from the thylakoid membrane lipid sulfoquinovosyl diacylglycerol, is abundant in photosynthetic organisms and plays a key role in global sulfur cycling. Its degradation in nature is mediated by specialized bacteria, many of which rely on the enzyme sulfoquinovosidase (YihQ) to release sulfoquinovose from sulfoquinovosyl (diacyl)glycerol. Despite its ecological importance, the diversity and functional roles of sulfoquinovose-degrading microorganisms remain poorly characterized in natural environments. Here, we developed a yihQ-targeted amplicon sequencing approach to investigate the richness and distribution of SQ-degrading bacteria across selected environments. We revealed high richness of yihQ-containing microorganisms in the analyzed cow rumen samples, far exceeding that observed in human and mouse gut microbiomes, suggesting an important role of sulfoquinovose metabolism in ruminant digestion. Anoxic microcosm experiments with sulfoquinovose-amended rumen fluid revealed cooperative microbial degradation of sulfoquinovose to sulfide via isethionate cross-feeding. Amplicon sequencing and genome-resolved metagenomics and metatranscriptomics identified yet undescribed and uncultured sulfoquinovose-degrading taxa. Members of Caproiciproducens (Acutalibacteraceae), Candidatus Limivicinus (Oscillospiraceae), and Sphaerochaetaceae transcribed the isethionate-producing sulfo-transketolase pathway, whereas isethionate was likely respired by a Candidatus Mailhella bacterium (Desulfovibrionaceae). This study presents a functional gene-based assay for tracking environmental yihQ richness, highlights sulfoquinovose degradation as a central metabolic process in the cow rumen, describes previously unknown sulfoquinovose-metabolizing bacteria, and advances understanding of sulfur physiology in complex microbial communities.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"109 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147524343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nutritional Pressure from Serum Amplifies Dysbiotic Features in an Oral Microbiome Synthetic Community 血清的营养压力放大了口腔微生物合成群落的生态失调特征

The ISME Journal Pub Date : 2026-03-26 DOI: 10.1093/ismejo/wrag070

Lu Li, Matthew Smardz, Dam Soh, Philip D Marsh, Anilei Hoare, Patricia I Diaz

{"title":"Nutritional Pressure from Serum Amplifies Dysbiotic Features in an Oral Microbiome Synthetic Community","authors":"Lu Li, Matthew Smardz, Dam Soh, Philip D Marsh, Anilei Hoare, Patricia I Diaz","doi":"10.1093/ismejo/wrag070","DOIUrl":"https://doi.org/10.1093/ismejo/wrag070","url":null,"abstract":"Despite rapid advances in characterizing the human microbiome, the ecological pressures shaping its transitions from healthy to diseased states remain poorly resolved. This is particularly true for periodontitis, a slow-progressing chronic inflammatory disease associated with well-defined shifts in the subgingival microbiome. Here, we report the development of a complex synthetic community model of the subgingival microbiome, designed for systematic interrogation of ecological factors that drive community restructuring. The model includes 22 prevalent and abundant subgingival species maintained in mucin-rich medium under microaerophilic, continuous culture conditions, in a chemostat. Using this system, we interrogated the impact of serum, as a surrogate for the inflammatory exudate produced by the host in response to biofilm accumulation, on community structure and function. Through integrated 16S rRNA gene sequencing, metatranscriptomics, and metabolomics, we found that serum was not required for a community with a periodontitis-like configuration to establish, but its presence intensified features of dysbiosis. Serum increased total biomass, promoted polymicrobial aggregate formation, promoted nitrogen and protein metabolism thereby modifying the environmental pH towards alkalinity, and introduced nitrosative stress. Serum also modified the community metatranscriptome in ways that paralleled microbiome activities in human periodontitis. Serum, however, decreased community diversity by disproportionally conferring a competitive advantage to the pathogen Porphyromonas gingivalis. This synthetic community model has revealed serum as a key nutritional pressure that modulates subgingival microbiome ecology and may perpetuate dysbiosis.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"318 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147524344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell assembled genomes predict enhanced bacterial metabolic cross-feeding potential in carbon-enriched soils 单细胞组装基因组预测富碳土壤中细菌代谢交叉摄食潜力的增强

The ISME Journal Pub Date : 2026-03-26 DOI: 10.1093/ismejo/wrag071

Hanyue Guo, Qicheng Xu, He Zhang, Yizhu Qiao, Yang Song, Yinghua Duan, Ning Ling, Qirong Shen

{"title":"Single-cell assembled genomes predict enhanced bacterial metabolic cross-feeding potential in carbon-enriched soils","authors":"Hanyue Guo, Qicheng Xu, He Zhang, Yizhu Qiao, Yang Song, Yinghua Duan, Ning Ling, Qirong Shen","doi":"10.1093/ismejo/wrag071","DOIUrl":"https://doi.org/10.1093/ismejo/wrag071","url":null,"abstract":"Carbon availability is a key determinant of soil microbial community structure and function, shaping their metabolic activities and interactions. However, the mechanisms driving these interactions and their ecological and evolutionary implications remain poorly understood. Here, we integrated single-cell Cell Sorting and Sequencing (scCS-seq) technologies with community metabolic modeling to investigate the genomic traits and metabolic interactions of microorganisms in soils with different carbon availability. We find that microorganisms in carbon-enriched soils exhibit larger genomes with higher coding sequence and enrichment of biosynthesis-related CAZyme families (e.g., GT83, GT44), whereas those in carbon-depleted soils adapt to resource scarcity with streamlined genomes and higher GC content. Our metabolic modeling predicts a stronger potential for cross-feeding in carbon-enriched soils, with amino acids and aromatic compounds identified as preferentially exchanged metabolites. This enhanced cross-feeding potential may promote resource sharing and functional complementarity among soil microorganisms. These findings highlight the role of microbial metabolic interactions as fundamental drivers of community assembly and ecosystem functioning, providing new insights into the ecological and evolutionary principles that structure soil microbiomes.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147524345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

AHL-mediated quorum sensing drives plastisphere formation and elevates pathogenic potential. ahl介导的群体感应驱动塑料球形成并提高致病潜力。

The ISME Journal Pub Date : 2026-03-24 DOI: 10.1093/ismejo/wrag066

Jie Wang,Lijia Lu,Yuanze Sun,Lauren F Messer,Mochen Wu,Zhuoran Duan,Jia Shi,Yuyi Yang,Changchao Li,Yanping Mao,Dong Zhu,Matthias C Rillig,Xiaoping Wang

{"title":"AHL-mediated quorum sensing drives plastisphere formation and elevates pathogenic potential.","authors":"Jie Wang,Lijia Lu,Yuanze Sun,Lauren F Messer,Mochen Wu,Zhuoran Duan,Jia Shi,Yuyi Yang,Changchao Li,Yanping Mao,Dong Zhu,Matthias C Rillig,Xiaoping Wang","doi":"10.1093/ismejo/wrag066","DOIUrl":"https://doi.org/10.1093/ismejo/wrag066","url":null,"abstract":"The biofilm colonizing plastic debris, termed 'the plastisphere', is of growing global concern due to escalating plastic pollution. However, the biological mechanisms underpinning plastisphere formation remain poorly understood. Here, we analyzed public global metagenomes, revealing a significant enrichment of genes associated with quorum sensing (QS) and biofilm formation, with a pronounced signal for acyl-homoserine lactone (AHL) QS. Using controlled microfluidic and tubular column experiments, we further demonstrate that exogenous AHL actively promotes plastisphere formation, biomass accumulation, and extracellular polymeric substance production on microplastics, whereas a quorum quenching agent (AHL acylase) effectively inhibits these processes. Multi-omics analyses revealed that AHLs can transcriptionally activate genes involved in adhesion, motility, chemotaxis, and matrix production, fundamentally reshaping community structure, restructuring inferred microbial interaction networks, and driving community assembly toward stronger deterministic selection. AHL stimulation also increased the relative abundance and expression of pathogen-associated and virulence-related functions, suggesting an elevated virulence potential within the plastisphere under QS-promoting conditions. Together, our findings establish AHL-mediated QS as a central driver of plastisphere assembly and a key determinant of risk profile, highlighting its critical role in understanding and potentially mitigating the growing environmental and health hazards associated with microplastic pollution.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host species-specific gene expression by a widespread and flexible chemosynthetic symbiont. 宿主物种特异性基因表达的广泛和灵活的化学合成共生体。

The ISME Journal Pub Date : 2026-03-24 DOI: 10.1093/ismejo/wrag065

A Carlotta Kück,Lukas Leibrecht,Isidora Morel-Letelier,Olivier Gros,Laetitia G E Wilkins,Benedict Yuen-Simović,Jillian M Petersen

{"title":"Host species-specific gene expression by a widespread and flexible chemosynthetic symbiont.","authors":"A Carlotta Kück,Lukas Leibrecht,Isidora Morel-Letelier,Olivier Gros,Laetitia G E Wilkins,Benedict Yuen-Simović,Jillian M Petersen","doi":"10.1093/ismejo/wrag065","DOIUrl":"https://doi.org/10.1093/ismejo/wrag065","url":null,"abstract":"Associations with microbial symbionts shape the ecology and evolution of almost all eukaryotes. One of their defining features is their specificity, but despite this, many symbioses show a degree of flexibility, with some symbiont species capable of colonizing multiple (often closely related) host species. Although widespread, the functional and evolutionary consequences of flexibility in host-symbiont pairings is poorly understood. Bivalves from the diverse, globally distributed, and ecologically important family Lucinidae are ideal for investigating this, as multiple host species can associate with the same symbiont species, often at the same location. We used metatranscriptomics to investigate the molecular responses of one symbiont species, Candidatus Thiodiazotropha endolucinida, in association with three different host species that co-occur in seagrass meadows in the Caribbean Sea. In replicated experiments, we identified host species-specific patterns of symbiont gene expression including those for key functions such as carbon fixation, cell division, and sulfide oxidation. Our work shows that the symbiont consistently responds in different ways to association with different host species. Because all samples were collected at the same site on the same day, and were thus exposed to the same environmental conditions, these differences are likely driven by host rather than environmental factors. In addition, host species had significantly different carbon isotope signatures, which were consistent with distinct modes of host-microbe interaction indicated by transcriptomics. Our results show that not only symbiont genotype, but also symbiont phenotype may enable coexistence of closely related host species, demonstrating the power of symbiosis in promoting and maintaining biodiversity.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"233 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two Vibrio species co-colonize a morphologically complex symbiotic light organ. 两种弧菌共同定殖形态复杂的共生光器官。

The ISME Journal Pub Date : 2026-03-24 DOI: 10.1093/ismejo/wrag063

Clotilde Bongrand,Raphael Lami,Marcelino T Suzuki,Eric J Koch

{"title":"Two Vibrio species co-colonize a morphologically complex symbiotic light organ.","authors":"Clotilde Bongrand,Raphael Lami,Marcelino T Suzuki,Eric J Koch","doi":"10.1093/ismejo/wrag063","DOIUrl":"https://doi.org/10.1093/ismejo/wrag063","url":null,"abstract":"The squid-vibrio symbiosis has illuminated fundamental mechanisms of beneficial animal-microbe associations, yet the interactions within sepiolid squid of the Mediterranean Sea remain underexplored. Here we characterize the Sepiola affinis squid-vibrio symbiosis by combining whole-genome sequencing of light-organ isolates, confocal microscopy, and temperature-dependent growth assays. Comparative genomic analyses (ANI, phylogenomics, and functional analyses) revealed two previously undescribed Vibrio species to be symbionts of the S. affinis light organ. One of the species clusters more distantly from other Vibrio species, whereas the second is closer to established Vibrio clades and exhibits an expanded repertoire of mobile elements and Type VI secretion components, suggesting heightened capacity for genetic exchange and interbacterial interaction. Furthermore, confocal microscopy of juvenile squid established that the S. affinis light organ comprises twelve crypts connected by pores and ducts, expanding the number of symbiotic niches relative to other sepiolid squid. In addition, fluorescently labeled isolates from the two Vibrio species colonized juveniles in both mono- and co-colonization patterns within crypts. Finally, growth assays across 16-24°C identified species-specific temperature differences, indicating temperature preferences that may align with seasonal variability in the Mediterranean Sea. Together, these findings position S. affinis to be a tractable model for studying how symbiont diversity, organ architecture, and interbacterial interactions contribute to the stability of a mutualistic symbiosis.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"20 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0