The ISME Journal最新文献

Microbiome spatial scaling varies among members, hosts, and environments across model island ecosystems. 微生物组的空间尺度在模型岛屿生态系统的成员、宿主和环境之间有所不同。

The ISME Journal Pub Date : 2025-10-13 DOI: 10.1093/ismejo/wraf228

Jason L Baer,Kacie T Kajihara,Leena L Vilonen,Allie J Hall,Cadie M Young,Danyel K Yogi,Matthew C I Medeiros,Anthony S Amend,Nicole A Hynson

{"title":"Microbiome spatial scaling varies among members, hosts, and environments across model island ecosystems.","authors":"Jason L Baer,Kacie T Kajihara,Leena L Vilonen,Allie J Hall,Cadie M Young,Danyel K Yogi,Matthew C I Medeiros,Anthony S Amend,Nicole A Hynson","doi":"10.1093/ismejo/wraf228","DOIUrl":"https://doi.org/10.1093/ismejo/wraf228","url":null,"abstract":"The species area relationship is a classic ecological law describing the relationship between habitat increase and the number of species. Species area relationships are resoundingly positive across macrobes such as plants and animals, and emerge through non-exclusive stochastic and deterministic processes including changes in immigration and extinction, drift, and environmental heterogeneity. Due to unique attributes of the microbial lifestyle, they may not abide by similar rules as macrobes, especially when it comes to spatial scaling. We predict that host-associated microbiomes will exhibit shallower species area relationships than free-living microbiomes due to strong host filtering, and that the species area relationships of bacteria will be shallower than fungi due primarily to differences in dispersal ability. We test these predictions in a relatively simple field system where bromeliad phytotelmata comprise aquatic ecosystems that support invertebrates and environmental substrates such as detritus. Larger phytotelmata generate larger habitat islands for microbiomes allowing us to explicitly examine their species area relationships. We find that the species area relationships of free-living and host-associated microbiomes differ, as do those of microbiome members. By assessing the relationship between environmental conditions and richness, and measuring diversity across scales, we posit that these observed differences in species area relationships are owed to differences in realized niches and dispersal abilities among microbes. These findings highlight that the classic laws of biological spatial scaling do not necessarily accurately represent microbiomes, and that the influence of area on diversity appears to be more important for some microbiomes and microbes than others.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"136 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal regimes during overwintering recovery shape microbial network and dissolved organic matter complexity in Microcystis-dominated systems. 在微囊藻主导的系统中，越冬恢复期间的热状态决定了微生物网络和溶解有机质的复杂性。

The ISME Journal Pub Date : 2025-10-13 DOI: 10.1093/ismejo/wraf227

Yang Liu,Zongjie Xie,Jia Feng,Shulian Xie,Chao Ma

{"title":"Thermal regimes during overwintering recovery shape microbial network and dissolved organic matter complexity in Microcystis-dominated systems.","authors":"Yang Liu,Zongjie Xie,Jia Feng,Shulian Xie,Chao Ma","doi":"10.1093/ismejo/wraf227","DOIUrl":"https://doi.org/10.1093/ismejo/wraf227","url":null,"abstract":"The overwintering recovery of Microcystis aeruginosa represents a critical but underexplored phase in the seasonal development of cyanobacterial blooms. Although the role of temperature in driving bloom onset is recognized, its effects on microbial assembly and the molecular transformation of dissolved organic matter during reactivation remain insufficiently characterized. In this study, 16S rRNA gene sequencing, excitation-emission matrix fluorescence spectroscopy coupled with parallel factor analysis, Fourier transform ion cyclotron resonance mass spectrometry, and metabolomics were applied to examine how three thermal recovery regimes-constant temperature, gradual warming, and cold-dark preconditioning-shape microbial succession and dissolved organic matter dynamics. Constant temperature accelerated the dispersal limitation of bacterial communities and promoted rapid DOM turnover, whereas gradual warming and cold-dark preconditioning induced more undominated community structures, and the accumulation of nitrogen- and sulfur-rich DOM compounds. Cold-dark pretreatment notably enhanced the formation of structurally complex, recalcitrant DOM, and delayed microbial reactivation. The network of relationships between microorganisms and dissolved organic matter revealed distinct coupling patterns across treatments, with enhanced microbial processing of aromatic and humic-like molecules occurring under thermal fluctuation or stress. Metabolomic profiling further indicated different physiological adaptation strategies, with stress-linked metabolites enriched under variable-temperature conditions. These findings highlight the mechanistic links between temperature-driven microbial recovery and dissolved organic matter transformation, providing new insights into how winter conditions influence cyanobacterial bloom trajectories in freshwater ecosystems.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145277133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Habitat-specificity in SAR11 is associated with a few genes under high selection SAR11的生境特异性与少数高选择基因有关

The ISME Journal Pub Date : 2025-10-11 DOI: 10.1093/ismejo/wraf216

Sarah J Tucker, Kelle C Freel, A Murat Eren, Michael S Rappé

{"title":"Habitat-specificity in SAR11 is associated with a few genes under high selection","authors":"Sarah J Tucker, Kelle C Freel, A Murat Eren, Michael S Rappé","doi":"10.1093/ismejo/wraf216","DOIUrl":"https://doi.org/10.1093/ismejo/wraf216","url":null,"abstract":"The order Pelagibacterales (SAR11) is the most abundant group of heterotrophic bacteria in the global surface ocean, where individual sublineages likely play distinct roles in oceanic biogeochemical cycles. Yet, understanding the determinants of niche partitioning within SAR11 has been a formidable challenge due to the high genetic diversity within individual SAR11 sublineages and the limited availability of high-quality genomes from both cultivation and metagenomic reconstruction. Through an integrated metapangenomic analysis of 71 new SAR11 isolate genomes and a time-series of metagenomes from the prominent source of isolation, we reveal an ecological and phylogenetic partitioning of metabolic traits across SAR11 genera. We resolve distinct habitat preferences among genera for coastal or offshore environments of the tropical Pacific and identify a handful of genes involved in carbon and nitrogen metabolisms that appear to contribute to these contrasting lifestyles. Furthermore, we find that some habitat-specific genes experience high selective pressures, indicating that they are critical determinants of SAR11 fitness and niche differentiation. Together, these insights reveal the underlying evolutionary processes shaping niche-partitioning within sympatric and parapatric populations of SAR11 and demonstrate that the immense genomic diversity of SAR11 bacteria naturally segregates into ecologically and genetically cohesive units, or ecotypes, that vary in spatial distributions in the tropical Pacific.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145261600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-cultivation rescues suicidal Paenibacillus amylolyticus swarms. 共同培养拯救了具有自杀倾向的溶淀粉芽孢杆菌群。

The ISME Journal Pub Date : 2025-10-09 DOI: 10.1093/ismejo/wraf225

Dana Ronin,Mads Frederik Hansen,Mette Burmølle

{"title":"Co-cultivation rescues suicidal Paenibacillus amylolyticus swarms.","authors":"Dana Ronin,Mads Frederik Hansen,Mette Burmølle","doi":"10.1093/ismejo/wraf225","DOIUrl":"https://doi.org/10.1093/ismejo/wraf225","url":null,"abstract":"Bacterial locomotion is integral to acquiring resources and getting access to new niches. Swarming, a type of motility where flagellated bacteria cooperatively move together across a semi solid surface, is one example of how bacteria can colonize new territories. This collective behavior is temporally and spatially orchestrated, requiring task specialization of community members. In this study, we paired a swarming bacterium, Paenibacillus amylolyticus, with a non-swarmer, Stenotrophomonas maltophilia, to investigate the impact on fitness of each strain. In dual-species conditions, the community swarm became significantly thicker and improved the ability of S. maltophilia to range into new territories. Swarming enabled P. amylolyticus to cross barriers of antimicrobials, whereas the thicker, dual-species swarm did not empower S. maltophilia to cross. Comparative studies of population dynamics revealed that over time, monospecies swarms of P. amylolyticus entered a state unable to grow despite still showing reductase activity. However, in a dual-species swarm, S. maltophilia rescued P. amylolyticus from this state. This rescue is attributed to the pH stabilization that occurs in this two-species combination, where S. maltophilia alkalizes the environment, thereby providing a more favorable environment for P. amylolyticus.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exceptionally high carbon fixation and nitrogen assimilation rates in microbial mats of an alkaline soda lake. 碱性钠湖微生物垫区异常高的固碳和氮同化率。

The ISME Journal Pub Date : 2025-10-09 DOI: 10.1093/ismejo/wraf226

Yihua Liu,Alyse K Kiesser,Agasteswar Vadlamani,Angela Kouris,Marc Strous

{"title":"Exceptionally high carbon fixation and nitrogen assimilation rates in microbial mats of an alkaline soda lake.","authors":"Yihua Liu,Alyse K Kiesser,Agasteswar Vadlamani,Angela Kouris,Marc Strous","doi":"10.1093/ismejo/wraf226","DOIUrl":"https://doi.org/10.1093/ismejo/wraf226","url":null,"abstract":"Alkaline soda lakes, characterized by high pH and high concentrations of sodium and dissolved carbonates, support diverse alkaliphilic microbial communities. Using stable isotope probing with 13C-bicarbonate, 15N-ammonium, 15N-nitrate, and 15N-urea, we measured assimilation rates for carbon and nitrogen by microbial mats of alkaline Goodenough Lake, Canada. Our results showed extremely high carbon fixation rates averaging 24 g C/m2/day, equalling or exceeding rates measured fifty years ago in African alkaline soda lakes. Urea consumption occurred both during the day and during the night, but assimilation mainly occurred during the day. Ammonium assimilation was stable between day and night. Apparently, cyanobacteria preferred urea as a nitrogen source, whereas heterotrophs preferred ammonium. Two different cyanobacteria dominated the microbial mats, Nodosilinea and Sodalinema. Using Orbitrap mass spectrometry, we only observed assimilation of 13C bicarbonate by Sodalinema, but not by Nodosilinea. The latter might focus on different carbon sources, such as urea. Strong negative correlation between their abundances in proteomes also supported niche partitioning between these two cyanobacteria.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"111 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High strain-level diversity of Bradyrhizobium across Australian soils. 澳洲土壤中缓生根瘤菌的高品系多样性。

The ISME Journal Pub Date : 2025-10-08 DOI: 10.1093/ismejo/wraf222

Clifton P Bueno de Mesquita,Matthew R Olm,Andrew Bissett,Noah Fierer

{"title":"High strain-level diversity of Bradyrhizobium across Australian soils.","authors":"Clifton P Bueno de Mesquita,Matthew R Olm,Andrew Bissett,Noah Fierer","doi":"10.1093/ismejo/wraf222","DOIUrl":"https://doi.org/10.1093/ismejo/wraf222","url":null,"abstract":"Global surveys of soil bacteria have identified several taxa that are nearly ubiquitous and often the most abundant members of soil bacterial communities. However, it remains unclear why these taxa are so abundant and prevalent across a wide range of soil types and environmental conditions. Here we use genome-resolved metagenomics to test the hypothesis that strain-level differences exist in these taxa that are not adequately captured with standard marker gene sequencing, and that distinct strains harbor unique traits that reflect adaptations to different soil environments. We analyzed data from 331 natural soils spanning Australia to assess strain differentiation in Bradyrhizobium, a dominant soil bacterial genus of ecological importance. We developed a workflow for strain-level bacterial analyses of complex soil metagenomes, combining genomes from pre-existing databases with new genomes generated via targeted assembly from metagenomes to detect 181 Bradyrhizobium strains across the soil collection. In addition to a high degree of phylogenetic variation, we observed substantial variation in pangenome content and inferred traits, highlighting the breadth of diversity within this widespread genus. Although members of the genus Bradyrhizobium were detected in >80% of samples, most individual strains were restricted in their distributions. The overall strain-level community composition of Bradyrhizobium varied significantly across geographic space and environmental gradients, and was particularly associated with differences in temperature, soil pH, and soil nitrate and metal concentrations. Our work provides a general framework for studying the strain-level ecology of soil bacteria and highlights the ecological and pangenomic diversity within this dominant soil bacterial genus.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon source diversity shapes bacterial interspecies interactions 碳源多样性决定了细菌种间的相互作用

The ISME Journal Pub Date : 2025-10-08 DOI: 10.1093/ismejo/wraf224

Hiroki Ono, Saburo Tsuru, Chikara Furusawa

{"title":"Carbon source diversity shapes bacterial interspecies interactions","authors":"Hiroki Ono, Saburo Tsuru, Chikara Furusawa","doi":"10.1093/ismejo/wraf224","DOIUrl":"https://doi.org/10.1093/ismejo/wraf224","url":null,"abstract":"Bacterial communities exhibit various classes of interspecies interactions, ranging from synergistic to competitive. As these interaction classes play a crucial role in determining characteristics of bacterial communities, including species composition and community stability, understanding the mechanisms that shape them is important. Whereas several studies have suggested that synergistic interactions are rare, a study focused on single-carbon-source environments reported them to be relatively common. This discrepancy highlights the potential role of carbon source diversity in shaping interaction classes, although the quantitative relationship remains unclear. To elucidate this relationship, we examined 896 interspecies interactions among 28 synthetic bacterial pairs, isolated from various environments, under 32 conditions with varying levels of carbon source diversity. As a result, we frequently observed synergistic interactions in single-carbon-source environments, with the interactions shifting to competitive as the carbon source diversity increased. Further analyses suggested that this shift was driven by processes occurring in environments with an increased diversity of carbon sources, such as resource competition. Our findings provide new insights into how environmental factors, particularly carbon source diversity, shape interspecies interactions in bacterial communities.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SAR11 ecotypes across ocean basins change with depth due to changes in light and oxygen. 由于光和氧的变化，整个海洋盆地的SAR11生态型随深度而变化。

The ISME Journal Pub Date : 2025-10-08 DOI: 10.1093/ismejo/wraf221

Matthew D Hays,Clara A Fuchsman

{"title":"SAR11 ecotypes across ocean basins change with depth due to changes in light and oxygen.","authors":"Matthew D Hays,Clara A Fuchsman","doi":"10.1093/ismejo/wraf221","DOIUrl":"https://doi.org/10.1093/ismejo/wraf221","url":null,"abstract":"SAR11 bacteria are ubiquitous and abundant heterotrophs that are important mediators of marine biogeochemical cycles. Within the SAR11 clade smaller ecotypes inhabit different ecological niches. Using metagenomic read placement onto a phylogenetic tree of RNA polymerase (rpoB), we were able to determine the distribution of different ecotypes both geographically and by depth. Our method avoids biases from the absence of quality sequenced genomes for deep SAR11 ecotypes. Depth profiles that range from the surface to the bathypelagic were analyzed at 30 stations in 6 ocean basins. In the euphotic zone, changes in the dominant primary producer from eukaryotic algae to cyanobacteria, did not cause the abundance of SAR11 to shift between stations. However, specific SAR11 ecotypes did correlate with eukaryotic phytoplankton (1a.3 and 1a.4) or picocyanobacteria (1b.2, 1b.4, and IIaB). In the lower euphotic and mesopelagic zones, group IIb.x was overwhelmingly the dominant species but group 1c was also present, and we found several new deep sub-ecotypes of 1b. The shift between the surface SAR11 community, dominated by 1a and surface 1b sub-ecotypes, and the mesopelagic ecotype groups, corresponded to the maximum decrease in the light-dependent proteorhodopsin/rpoB ratio, indicating that many deep ecotypes did not possess proteorhodopsin. This ecotype switch repeatedly corresponded to the maximum in Low Light I Prochlorococcus, leading to the hypothesis that changes in light motivates the ecotype switch. Environmentally abiotic factors like light and temperature appear to be determining factors in the SAR11 ecotype distribution throughout the global oceans.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and Evolution of Prokaryotic Viral Lytic Proteins. 原核病毒裂解蛋白的多样性和进化。

The ISME Journal Pub Date : 2025-10-08 DOI: 10.1093/ismejo/wraf200

Ting Yang,Mujie Zhang,Yi Yi,Yecheng Wang,Zhiwei Wang,Rui Zhang,Xiang Xiao,Huahua Jian

{"title":"Diversity and Evolution of Prokaryotic Viral Lytic Proteins.","authors":"Ting Yang,Mujie Zhang,Yi Yi,Yecheng Wang,Zhiwei Wang,Rui Zhang,Xiang Xiao,Huahua Jian","doi":"10.1093/ismejo/wraf200","DOIUrl":"https://doi.org/10.1093/ismejo/wraf200","url":null,"abstract":"Lytic proteins, essential for viral life cycles, mediate cell lysis, driving nutrient and gene flow in ecosystems. Despite advances in understanding viral lysis mechanisms, the lytic proteins of prokaryotic viruses remain poorly understood at the macroevolutionary scale. Here, we constructed the Prokaryotic DNA Virus Lytic Protein Dataset, revealing the diversity, distribution patterns, and evolutionary drivers of lytic proteins across viral genomes. Our results demonstrate sequence and structural variation, suggesting that the composition of the lysis system is closely linked to viral genome size, host cell wall structure, and lifestyle, reflecting ecological adaptation. We observed that viral lytic proteins exhibit extensive sequence variation but retain structural conservation, suggesting a stronger selective pressure on structure that may be driven by the need to adapt and conform with specific cell envelope architectures. Phylogenetic analyses identified a significant co-evolutionary signal among lytic proteins, alongside extensive horizontal gene transfer of endolysin and holin encoding genes between bacteriophages and bacteria. These analyses also support that viral lytic proteins likely originated from bacterial sources, with different functional types having multiple independent origins. Moreover, comparative analysis of DNA and RNA virus lytic proteins demonstrates their diversity and differences across viral lineages. Revealing vast unexplored lytic proteins diversity, this study highlights their biotechnological potential against multidrug-resistant pathogens.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145246825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic biodegradation of polyethylene by experimentally evolved bacterial biofilms 实验进化的细菌生物膜对聚乙烯的协同生物降解

The ISME Journal Pub Date : 2025-10-08 DOI: 10.1093/ismejo/wraf223

Shan Li, Jiajia Liu, Lei Su, Jingwen Qiu, Lianbing Lin, Ákos T Kovács, Yicen Lin

{"title":"Synergistic biodegradation of polyethylene by experimentally evolved bacterial biofilms","authors":"Shan Li, Jiajia Liu, Lei Su, Jingwen Qiu, Lianbing Lin, Ákos T Kovács, Yicen Lin","doi":"10.1093/ismejo/wraf223","DOIUrl":"https://doi.org/10.1093/ismejo/wraf223","url":null,"abstract":"Polyethylene, one of the most widely used synthetic polymers, presents significant environmental challenges due to its resistance to biodegradation. Its surface offers a unique ecological niche for microbial colonization and serves as a primary habitat for degrading microorganisms. Despite the pivotal role microbial communities play in plastic degradation, there has been limited research on constructing stable, interacting microbial consortia. In this study, we explored the potential of evolving bacterial biofilm communities to enhance polyethylene degradation. Through long-term experimental evolution, six microbial populations underwent 40 selection cycles using polyethylene as their sole carbon source. The resulting evolved communities formed robust, multi-species biofilms with enhanced degradation capabilities, outperforming their ancestral populations in biofilm production. Stutzerimonas stutzeri emerged as the dominant species, orchestrating a synergistic interaction with two other isolates through metabolic division of labor. (Meta)-transcriptomics analysis revealed that Stutzerimonas primarily contributed to the expression of enzymes involved in microbe-mediated degradation of polyethylene, whereas the other community members were responsible for secreting extracellular polysaccharides, improving biofilm formation. This study highlights the potential of experimentally evolved microbial consortia to synergistically accelerate plastic biodegradation, offering promising strategies for environmental bioremediation.","PeriodicalId":516554,"journal":{"name":"The ISME Journal","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145247046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0