Veterinary Quarterly最新文献_第8页

Extracellular vesicles secreted by equine adipose mesenchymal stem cells preconditioned with transforming growth factor β-1 are enriched in anti-fibrotic miRNAs and inhibit the expression of fibrotic genes in an in vitro system of endometrial stromal cells fibrosis. 经转化生长因子β-1预处理的马脂肪间充质干细胞分泌的胞外囊泡富含抗纤维化的miRNA，并能抑制子宫内膜基质细胞纤维化体外系统中纤维化基因的表达。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-07-31 DOI: 10.1080/01652176.2024.2384906

Yat Sen Wong, Ana Carolina Mançanares, Felipe Navarrete, Pamela Poblete, Lídice Mendez-Pérez, Joel Cabezas, Gonzalo Riadi, Lleretny Rodríguez-Alvarez, Fidel Ovidio Castro

{"title":"Extracellular vesicles secreted by equine adipose mesenchymal stem cells preconditioned with transforming growth factor β-1 are enriched in anti-fibrotic miRNAs and inhibit the expression of fibrotic genes in an in vitro system of endometrial stromal cells fibrosis.","authors":"Yat Sen Wong, Ana Carolina Mançanares, Felipe Navarrete, Pamela Poblete, Lídice Mendez-Pérez, Joel Cabezas, Gonzalo Riadi, Lleretny Rodríguez-Alvarez, Fidel Ovidio Castro","doi":"10.1080/01652176.2024.2384906","DOIUrl":"10.1080/01652176.2024.2384906","url":null,"abstract":"Mare endometrosis is a major reproductive problem associated with low fertility and is characterized by persistent inflammation, TGFβ-1 signaling, and consequently, extracellular matrix deposition, which compromises endometrial glands. Mesenchymal stem cell-based products (MSCs), such as extracellular vesicles (EVs), have gained attention due to the regulatory effects exerted by their miRNA cargo. Here, we evaluated the impact of preconditioning equine adipose mesenchymal stem cells with TGFβ-1 for short or long periods on the anti-fibrotic properties of secreted extracellular vesicles. MSCs were isolated from six healthy horses and exposed to TGFβ-1 for 4, 24, and 0 h. The expression of anti-fibrotic and pro-fibrotic miRNAs and mRNAs in treated cells and miRNAs in the cargo of secreted extracellular vesicles was measured. The resulting EVs were added for 48 h to endometrial stromal cells previously induced to a fibrotic status. The expression of anti-fibrotic and pro-fibrotic genes and miRNAs was evaluated in said cells using qPCR and next-generation sequencing. Preconditioning MSCs with TGFβ-1 for 4 h enriched the anti-fibrotic miRNAs (mir29c, mir145, and mir200) in cells and EVs. Conversely, preconditioning the cells for 24 h leads to a pro-fibrotic phenotype overexpressing mir192 and mir433. This finding might have implications for developing an EV-based protocol to treat endometrial fibrosis in mares.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-11"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11295685/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141861601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and quantification of Brucella abortus DNA in water buffaloes (bubalus bubalis) using droplet digital polymerase chain reaction. 利用液滴数字聚合酶链式反应检测和量化水牛体内流产布鲁氏菌的DNA

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-08-15 DOI: 10.1080/01652176.2024.2390944

Giovanna Fusco, Lorena Cardillo, Ornella Valvini, Alessia Pucciarelli, Gerardo Picazio, Anna Cerrone, Michele Napoletano, Roberta Pellicanò, Maria Ottaiano, Claudio de Martinis, Francesca De Falco, Anna Cutarelli, Emanuela Sannino, Giorgia Borriello, Manuela Tittarelli, Sante Roperto, Esterina De Carlo

{"title":"Detection and quantification of Brucella abortus DNA in water buffaloes (bubalus bubalis) using droplet digital polymerase chain reaction.","authors":"Giovanna Fusco, Lorena Cardillo, Ornella Valvini, Alessia Pucciarelli, Gerardo Picazio, Anna Cerrone, Michele Napoletano, Roberta Pellicanò, Maria Ottaiano, Claudio de Martinis, Francesca De Falco, Anna Cutarelli, Emanuela Sannino, Giorgia Borriello, Manuela Tittarelli, Sante Roperto, Esterina De Carlo","doi":"10.1080/01652176.2024.2390944","DOIUrl":"10.1080/01652176.2024.2390944","url":null,"abstract":"Brucellosis represents a major public health concern worldwide. Human transmission is mainly due to the consumption of unpasteurized milk and dairy products of infected animals. The gold standard for the diagnosis of Brucella spp in ruminants is the bacterial isolation, but it is time-consuming. Polymerase Chain Reaction (PCR) is a quicker and more sensitive technique than bacterial culture. Droplet digital PCR (ddPCR) is a novel molecular assay showing high sensitivity in samples with low amount of DNA and lower susceptibility to amplification inhibitors. Present study aimed to develop a ddPCR protocol for the detection of Brucella abortus in buffalo tissue samples. The protocol was validated using proficiency test samples for Brucella spp by real time qPCR. Furthermore, 599 tissue samples were examined. Among reference materials, qPCR and ddPCR demonstrated same performance and were able to detect up to 225 CFU/mL. Among field samples, ddPCR showed higher sensitivity (100%), specificity and accuracy of 93.4% and 94.15%, respectively. ddPCR could be considered a promising technique to detect B. abortus in veterinary specimens, frequently characterized by low amount of bacteria, high diversity in matrices and species and poor storage conditions.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-8"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11328813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141989496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacokinetics, optimal dosages and withdrawal time of amoxicillin in Nile tilapia (Oreochromis niloticus) reared at 25 and 30 °C. 尼罗罗非鱼（Oreochromis niloticus）在 25 和 30 摄氏度条件下饲养时阿莫西林的药代动力学、最佳剂量和停药时间。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2024-12-01 Epub Date: 2024-08-27 DOI: 10.1080/01652176.2024.2396573

Tirawat Rairat, Yi-Ping Lu, Wan-Cih Ho, Hual-Jhong Ke, Chi-Chung Chou

{"title":"Pharmacokinetics, optimal dosages and withdrawal time of amoxicillin in Nile tilapia (Oreochromis niloticus) reared at 25 and 30 °C.","authors":"Tirawat Rairat, Yi-Ping Lu, Wan-Cih Ho, Hual-Jhong Ke, Chi-Chung Chou","doi":"10.1080/01652176.2024.2396573","DOIUrl":"10.1080/01652176.2024.2396573","url":null,"abstract":"Knowledge of amoxicillin (AMX) pharmacokinetics (PK) and tissue residues in fish, which is necessary for prudent drug use, remains limited. The study aimed to explore the PK characteristics of AMX in Nile tilapia (Oreochromis niloticus) reared at 25 and 30 °C as well as to determine optimal dosages and drug withdrawal time (WDT). In the PK investigation, the fish received a single dose of 40 mg/kg AMX via oral gavage, and the optimal dosage was determined by the pharmacokinetic-pharmacodynamic approach. In the tissue residue study, the fish were orally gavaged with 40 mg/kg/day AMX once daily for 5 days and the WDT was established by the linear regression analysis. The results revealed the temperature-dependent drug elimination; the clearance relative to bioavailability (CL/F) and elimination half-life at 30 °C (0.180 L/kg/h and 6.06 h, respectively) were about twice those at 25 °C (0.090 L/kg/h and 10.49 h, respectively). The optimal dosages at the minimum inhibitory concentration (MIC) of 2 μg/mL were 10.97 (25 °C) and 41.03 (30 °C) mg/kg/day, respectively. Finally, following the multiple oral administration, the muscle/skin residue of AMX on day 1 after the last dosing at 25 and 30 °C were 548 and 264 ng/g, respectively. The average tissue residues were depleted below the maximum residue limits (MRL) of 50 μg/kg on day 5 (25 °C) and 3 (30 °C), respectively, and the WDT were 6 and 4 days when rearing at 25 and 30 °C, respectively. This knowledge serves as a practical guideline for responsible use of AMX in treating bacterial diseases in Nile tilapia aquaculture.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"44 1","pages":"1-9"},"PeriodicalIF":7.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11360631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictive value of TP53 RNAscope®in situ hybridization and p53 immunohistochemistry for TP53 mutational status in canine diffuse large B-cell lymphoma. TP53 RNAscope® 原位杂交和 p53 免疫组织化学对犬弥漫大 B 细胞淋巴瘤 TP53 突变状态的预测价值。

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-09-16 DOI: 10.1080/01652176.2024.2403453

Greta Foiani,Luca Licenziato,Laura Marconato,Antonella Fanelli,Erica Melchiotti,Claudia Zanardello,Luca Aresu,Marta Vascellari

{"title":"Predictive value of TP53 RNAscope®in situ hybridization and p53 immunohistochemistry for TP53 mutational status in canine diffuse large B-cell lymphoma.","authors":"Greta Foiani,Luca Licenziato,Laura Marconato,Antonella Fanelli,Erica Melchiotti,Claudia Zanardello,Luca Aresu,Marta Vascellari","doi":"10.1080/01652176.2024.2403453","DOIUrl":"https://doi.org/10.1080/01652176.2024.2403453","url":null,"abstract":"TP53 mutations are associated with short survival and poor treatment response in canine diffuse large B-cell lymphoma (cDLBCL). The expression of TP53 by RNAscope® in situ hybridization and p53 by immunohistochemistry (IHC) was investigated in 37 formalin-fixed paraffin-embedded cDLBCL, to assess their correlation with TP53 mutational status and to evaluate their prognostic value. TP53 was detected in all samples by RNAscope®. Ten of 37 (27%) cases expressed p53 by IHC, with highly variable percentage of positive cells. TP53 RNAscope® scores and p53 IHC results were not correlated. The expression of TP53 by RNAscope® was not influenced by its mutational status. Conversely, p53 IHC and TP53 mutations were significantly associated. p53 IHC predicted TP53 genetic mutations with high accuracy (97.3%). All TP53-mutated samples carrying missense mutations exhibited p53 expression by IHC, while all wild-type cases and a single case with frameshift insertion were negative. In univariable analysis, p53 IHC was associated with shorter time to progression (TTP) and lymphoma-specific survival (LSS). Nevertheless, in multivariable analysis, only treatment significantly affected TTP and LSS. These findings suggest p53 IHC is an accurate, cost-effective tool for predicting TP53 mutations in cDLBCL, unlike TP53 RNAscope®, though its prognostic value requires further validation.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"41 1","pages":"1-9"},"PeriodicalIF":6.4,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142251241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the feasibility of equine in-vivo ultrasound technique for the medial branch of the dorsal ramus of the cervical spinal nerves 评估颈脊神经背侧支内侧支马体内超声技术的可行性

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-09-13 DOI: 10.1080/01652176.2024.2403456

Irene Nocera, Chiara Di Franco, Benedetta Sorvillo, Benedetta Aliboni, Elena Bucchioni, Micaela Sgorbini, Giulia Sala, Simonetta Citi

引用次数: 0

Modulating effects of heat-killed and live Limosilactobacillus reuteri PSC102 on the immune response and gut microbiota of cyclophosphamide-treated rats 热杀死和活的Limosilactobacillus reuteri PSC102对环磷酰胺治疗大鼠的免疫反应和肠道微生物群的调节作用

IF 6.4 2区农林科学

Veterinary Quarterly Pub Date : 2024-04-29 DOI: 10.1080/01652176.2024.2344765

Md. Sekendar Ali, Eon-Bee Lee, Yixian Quah, Syed Al Jawad Sayem, Muhammad Aleem Abbas, Kyoungho Suk, Seung-Jin Lee, Seung-Chun Park

引用次数: 0

The forecasting power of the mucin-microbiome interplay in livestock respiratory diseases 牲畜呼吸道疾病中粘蛋白-微生物组相互作用的预测能力