Veterinary Quarterly最新文献_第7页

Evaluation of recombinant extracellular enveloped virion protein candidates for the detection of serological responses to lumpy skin disease virus in cattle. 重组细胞外包膜病毒粒子候选蛋白检测牛肿块性皮肤病病毒血清学反应的评价。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-19 DOI: 10.1080/01652176.2025.2475989

Kitipong Angsujinda, Phatpimol Kitchanakan, Nabhasbhichayabha Daewang, Lerdchai Chintapitaksakul, Saruda Wanganurakkul, Sudkate Chaiyo, Nanthika Khongchareonporn, Timothy J Mahony, Wanchai Assavalapsakul

{"title":"Evaluation of recombinant extracellular enveloped virion protein candidates for the detection of serological responses to lumpy skin disease virus in cattle.","authors":"Kitipong Angsujinda, Phatpimol Kitchanakan, Nabhasbhichayabha Daewang, Lerdchai Chintapitaksakul, Saruda Wanganurakkul, Sudkate Chaiyo, Nanthika Khongchareonporn, Timothy J Mahony, Wanchai Assavalapsakul","doi":"10.1080/01652176.2025.2475989","DOIUrl":"10.1080/01652176.2025.2475989","url":null,"abstract":"Lumpy skin disease virus (LSDV) is a significant threat to cattle, particularly in countries like Thailand, where outbreaks have necessitated the importation of diagnostic kits and vaccines. This study aimed to evaluate several recombinant extracellular enveloped virion (EEV) protein candidates, including F13L, A33R, A34R, and B5R, for their potential use in serological detection assays for LSDV specific antibodies in cattle. Given the challenges associated with LSDV research, such as its classification as a Class III biological agent in Thailand, gene synthesis was employed to produce these proteins. The recombinant proteins were expressed in a prokaryotic system and analyzed using SDS-PAGE and Western blotting. Among the candidates, F13L demonstrated the highest correlation with the results from a commercially available and validated ELISA, yielding 85.7%, and 75% positive for the infected and vaccinated groups, respectively, identifying it a promising candidate for serosurveillance activities during active LSDV outbreaks. Sequence analysis confirmed a 100% match between the F13L designed from the Neethling type strain 2490 and various Thai LSDV strains from the 2021 outbreaks, underscoring its potential as a conserved diagnostic marker. The availability of recombinant F13L and its reactivity with cattle sera from LSDV infected or vaccinated animals, demonstrated in this study, suggests it could also serve as a potential candidate for vaccine development. The study concludes that recombinant F13L shows great promise for the development of LSDV serological assays, though further optimization and validation are necessary to harness its diagnostic potential.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11924265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143659452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Understanding zoonotic pathogens and risk factors from wildlife in Southeast Asia: a systematic literature review. 了解东南亚野生动物的人畜共患病病原体和危险因素：系统的文献综述。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-03-10 DOI: 10.1080/01652176.2025.2475990

Ha Thi Thanh Nguyen, Johanna F Lindahl, Bernard Bett, Hung Nguyen-Viet, Steven Lâm, Thang Nguyen-Tien, Fred Unger, Sinh Dang-Xuan, Thanh Xuan Bui, Hien Thanh Le, Åke Lundkvist, Jiaxin Ling, Hu Suk Lee

{"title":"Understanding zoonotic pathogens and risk factors from wildlife in Southeast Asia: a systematic literature review.","authors":"Ha Thi Thanh Nguyen, Johanna F Lindahl, Bernard Bett, Hung Nguyen-Viet, Steven Lâm, Thang Nguyen-Tien, Fred Unger, Sinh Dang-Xuan, Thanh Xuan Bui, Hien Thanh Le, Åke Lundkvist, Jiaxin Ling, Hu Suk Lee","doi":"10.1080/01652176.2025.2475990","DOIUrl":"10.1080/01652176.2025.2475990","url":null,"abstract":"The COVID-19 pandemic has demonstrated the significance of the human-animal interface in the emergence of zoonotic diseases, with wildlife serving as an important source of infection. A better understanding of the specific pathogens and mechanisms involved is vital to prepare against future outbreaks, especially in Southeast Asia, a hotspot for zoonotic diseases. This paper reviews the published literature on wildlife zoonoses in this region from 2012 to 2022. The results show a diverse range of potential zoonotic pathogens and the widespread occurrence of zoonotic diseases from wildlife. Drivers of zoonotic pathogen spillover include (i) environmental factors (e.g. animal habitat disruption, environmental conditions, exposure to contaminated water/food/soil), (ii) animal factors (e.g. movement patterns, age-related susceptibility), (iii) human factors (e.g. lack of awareness, poor hygiene practices, age, gender and income) and (iv) human-animal-environmental interface factors (e.g. close contact between humans and animals, exposure through visiting animals and presence of vectors). The diverse drivers of zoonoses in Southeast Asia put its communities at risk for infection. To mitigate these risks, global health efforts should consider adopting a One Health approach to foster collaboration across human, animal, and wildlife health sectors. This could involve educating communities on safe animal interactions and improving disease surveillance.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-17"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11894755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143587964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-intensity zones in dogs with lumbosacral intervertebral disc degeneration: insights from MRI and histopathological findings. 腰骶椎间盘退变犬的高强度区：来自MRI和组织病理学结果的见解。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-04-07 DOI: 10.1080/01652176.2025.2486765

S Amir Kamali, Michelle Teunissen, Dirk Hendrik Nicolaas van den Broek, Elisabeth M Burgers, Guy C M Grinwis, Keita Ito, Marianna A Tryfonidou, Björn P Meij

{"title":"High-intensity zones in dogs with lumbosacral intervertebral disc degeneration: insights from MRI and histopathological findings.","authors":"S Amir Kamali, Michelle Teunissen, Dirk Hendrik Nicolaas van den Broek, Elisabeth M Burgers, Guy C M Grinwis, Keita Ito, Marianna A Tryfonidou, Björn P Meij","doi":"10.1080/01652176.2025.2486765","DOIUrl":"10.1080/01652176.2025.2486765","url":null,"abstract":"The diagnosis and management of lumbosacral pain in dogs is challenging, requiring thorough examination, with MRI playing a crucial diagnostic role. This retrospective study investigates the presence of high-intensity zones (HIZ) in the dorsal annulus fibrosus (AF) of the lumbosacral region on MRI and describes the corresponding histopathological features in dogs with intervertebral disc (IVD) degeneration. T2-weighted (T2W) and T1-weighted (T1W) sagittal MRI scans were evaluated using a classification system developed in human medicine to analyze HIZ characteristics. Among 836 dogs with IVD degeneration, 57 (6.8%) exhibited T2W HIZ, with a median age of 7 years and median weight of 33.7 kg. All cases with HIZ consistently exhibited radiological degenerative lumbosacral stenosis. The most common T2W HIZ shape was round (43%), while 14% of lesions also appeared hyperintense on T1W. Histopathological analysis of 11 dorsal AF samples collected during standard-of-care decompressive surgery revealed two patterns: reactive cystic structures (3/11) and granulation tissue (8/11), with differential MRI presentation. This is the first study to document HIZ in the lumbosacral level of dogs with IVD degeneration. With this recognition, prospective analyses and their correlation with clinical presentations will be essential in determining the role and prognostic significance of HIZ.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perioperative drug management in non-critical companion animals: a retrospective study at a Spanish veterinary teaching hospital (2018-2022). 非关键伴侣动物围手术期药物管理：西班牙兽医教学医院回顾性研究（2018-2022）

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-06-06 DOI: 10.1080/01652176.2025.2513965

Beatriz Romero, Julen Susperregui, Ana M Sahagún, José R Altónaga, Cristina López, Raúl de la Puente, José M Rodríguez, Milena Vázquez, Raquel Díez

{"title":"Perioperative drug management in non-critical companion animals: a retrospective study at a Spanish veterinary teaching hospital (2018-2022).","authors":"Beatriz Romero, Julen Susperregui, Ana M Sahagún, José R Altónaga, Cristina López, Raúl de la Puente, José M Rodríguez, Milena Vázquez, Raquel Díez","doi":"10.1080/01652176.2025.2513965","DOIUrl":"10.1080/01652176.2025.2513965","url":null,"abstract":"There is little information in veterinary literature on the perioperative pharmacological management of small animal patients, despite the existence of common protocols and the importance of properly managing this period to reduce anaesthesia-related detrimental effects. This study aimed to analyse the current use of perioperative drugs in companion animals treated at the Veterinary Teaching Hospital of the University of León (HVULE) in Spain over a period of 5 years (2018-2022), describe the prescription patterns of these medicines, and identify the main variables associated with their prescription to explore possible strategies to promote their appropriateness. A total of 3438 cases were included in this study. The animals that most frequently underwent surgery were dogs (58.2%), females (57.0%), and adults (73.0%). The primary procedures performed were reproductive (56.6%) and traumatological (19.8%) surgeries. Regarding pharmacological treatments, more than half (62.3%) belonged to the ATCvet classification QN group (nervous system), and the most common compounds were isoflurane (13.5%), methadone (13.5%), and propofol (12.7%). Amoxicillin/clavulanic acid (3.7%) and marbofloxacin (2.8%) (categories C and B in the European Medicines Agency categorisation, respectively) were the most prescribed antibiotics. These findings provide detailed data to help veterinary policymakers improve drug use during surgical procedures.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-17"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12147486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples. 长期血清保存是否适合研究？时间和温度对犬血清抗利什曼原虫抗体测定的影响。

IF 7.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-07-15 DOI: 10.1080/01652176.2025.2532396

Diana Marteles, María Eugenia Lebrero, Sergio Villanueva-Saz, Clara Esteban Sanz, Víctor Martín, Antonio Fernández, Pablo Quilez, Maite Verde, Patricia Galan-Malo, M Dolores Pérez

{"title":"Is long-term serum preservation suitable for research studies? Effect of time and temperature on the measurement of anti-Leishmania antibodies in canine sera samples.","authors":"Diana Marteles, María Eugenia Lebrero, Sergio Villanueva-Saz, Clara Esteban Sanz, Víctor Martín, Antonio Fernández, Pablo Quilez, Maite Verde, Patricia Galan-Malo, M Dolores Pérez","doi":"10.1080/01652176.2025.2532396","DOIUrl":"10.1080/01652176.2025.2532396","url":null,"abstract":"The stability of immunoglobulin G (IgG) antibodies is critical for diagnostic and research applications in veterinary medicine. This study evaluated the long-term stability of anti-Leishmania infantum IgG in canine serum samples under different storage conditions (-20 °C and -80 °C) over 2.5 years. Fifty-six serum samples were classified based on antibody concentration into low, medium, and high positive groups using an in-house enzyme-linked immunosorbent assay. Each sample was divided into aliquots and analyzed after different storage times (6 months, 1 year, 1.5, and 2.5 years). No statistically significant differences were observed in IgG concentrations across storage durations or between storage temperatures. Median antibody levels remained consistent, with minor variations attributed to assay-related variability. Correlation analyses showed strong agreement between initial and final measurements (R2 = 0.859 at -20 °C, R2 = 0.957 at -80 °C). The study underscores the suitability of -20 °C and -80 °C storage for preserving anti-Leishmania antibodies, providing valuable insights for serological diagnostics and research in veterinary science. Proper sample handling and aliquoting are recommended to maintain antibody integrity in routine diagnostics and long-term studies.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"11-20"},"PeriodicalIF":7.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12265091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144638630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens. 建立鸡白痢沙门氏菌TaqMan qPCR检测方法。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-01-30 DOI: 10.1080/01652176.2025.2454473

Hao Wang, Xiamei Kang, Longhai Yu, Haijian Wang, Anja Müller, Corinna Kehrenberg, Yan Li, Min Yue

{"title":"Developing a novel TaqMan qPCR assay for optimizing Salmonella Pullorum detection in chickens.","authors":"Hao Wang, Xiamei Kang, Longhai Yu, Haijian Wang, Anja Müller, Corinna Kehrenberg, Yan Li, Min Yue","doi":"10.1080/01652176.2025.2454473","DOIUrl":"10.1080/01652176.2025.2454473","url":null,"abstract":"Salmonella Pullorum, the causative agent of pullorum disease, posing a significant threat to the global production of poultry meat and eggs. However, existing detection methods have substantial limitations in efficiency and accuracy. Herein, we developed a genomic deletion-targeted TaqMan qPCR assay for identification of Salmonella Pullorum, enabling precise differentiation from other Salmonella serovars. The assay's detection limit was 5 copies/μL of plasmid and 4 CFU/μL of bacterial DNA. Furthermore, we collected 676 chicken samples from an established infection model to compare the performance of the TaqMan qPCR assay with traditional bacterial culturing and antibody-based detection approaches. With superior sensitivity and specificity, the newly developed method detected over 80% of positive chickens, significantly outperforming the two conventional methods. Moreover, we proposed a combined framework that incorporates the advantages of TaqMan qPCR assay and antibody detection method, further enhancing the detection rate of positives to 92%. Additionally, to address the frequent aerosol contamination of amplification products in laboratory settings, we devised an easy-to-deploy anti-contamination system based on T7 exonuclease. Overall, the T7 exonuclease-assisted TaqMan qPCR assay will not only upgrade the current detection for pullorum disease, but also exemplify the feasibility of targeting specific genomic deletions for pathogen detection.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"1-13"},"PeriodicalIF":3.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11784030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary metabolite signatures as predictive biomarkers for estrus detection in water buffaloes: a proton-NMR based study. 尿液代谢物特征作为预测水牛发情的生物标志物：基于质子核磁共振的研究。

IF 3.9 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-12-13 DOI: 10.1080/01652176.2025.2593361

Suman Sangwan, M H Jan, Ekta Hooda, Renu Choudhary, Sunesh Balhara, Rupali Rautela, Sarita Yadav, S K Phulia, R K Sharma, Vijay Paul, Yash Pal, Mehar Singh Khatkar, Ashok Kumar Balhara

{"title":"Urinary metabolite signatures as predictive biomarkers for estrus detection in water buffaloes: a proton-NMR based study.","authors":"Suman Sangwan, M H Jan, Ekta Hooda, Renu Choudhary, Sunesh Balhara, Rupali Rautela, Sarita Yadav, S K Phulia, R K Sharma, Vijay Paul, Yash Pal, Mehar Singh Khatkar, Ashok Kumar Balhara","doi":"10.1080/01652176.2025.2593361","DOIUrl":"10.1080/01652176.2025.2593361","url":null,"abstract":"Developing a reliable, field ready estrus detection method is crucial for improving buffalo reproduction due to their subtle and poorly expressed estrus signs. This study investigated estrus phase-specific urinary metabolites in cyclic Murrah buffaloes (n=6) using proton nuclear magnetic resonance (1H-NMR). A total of 90 urinary metabolites were identified, with 15 consistently detected across all animals during the estrus phases (proestrus, estrus and diestrus). PCA highlighted p-cresol, ornithine, phenol, chlorogenate, quinolinate, hippurate and 2-hydroxyisobutyrate as key metabolites differentiating the estrus phases. PLS-DA identified p-cresol and phenol for estrus; chlorogenate and o-acetylcholine for proestrus and ornithine in diestrus as the potential urinary markers for detection of estrus phases based on their VIP scores greater than 1.5. Metabolic pathway analysis revealed that the glycerophospholipid pathway, phenylalanine, tyrosine and tryptophan biosynthesis, aspartate and aldarate metabolism, and starch and sucrose metabolism were the major metabolic pathways involved in regulating and controlling estrus cycle. Notably, p-cresol and phenol exhibited significant abundance during estrus (over 9-fold and 5-fold, respectively), suggesting their potential as putative estrus detection biomarkers. However, given the limited sample size (n = 6), these findings should be considered preliminary, and independent validation in larger, well-characterized cohorts is needed to confirm diagnostic utility.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"2593361"},"PeriodicalIF":3.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12915391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145745579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of an emerging canine respiratory coronavirus in China. 中国一种新型犬呼吸道冠状病毒的特征

IF 5.2 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-10-17 DOI: 10.1080/01652176.2025.2574506

Yunxin Ren, Jian Huang, Xi Chen, Cheng Tang, Hua Yue

{"title":"Characteristics of an emerging canine respiratory coronavirus in China.","authors":"Yunxin Ren, Jian Huang, Xi Chen, Cheng Tang, Hua Yue","doi":"10.1080/01652176.2025.2574506","DOIUrl":"10.1080/01652176.2025.2574506","url":null,"abstract":"Canine respiratory coronavirus (CRCoV) is a prevalent pathogen implicated in canine infectious respiratory disease, yet information on its genomic characteristics and pathogenicity remains scarce. To address this situation, we investigated the genetic evolution and pathogenic potential of CRCoV strains circulating in China. Five complete CRCoV genomes (GenBank: PQ725948-PQ725952) were obtained from clinical samples, and phylogenetic analysis showed these strains formed a distinct genetic branch. The evolutionary trees for ORF1ab, HE, and S genes closely mirrored the full genome tree, indicating key roles for these genes in CRCoV evolution. Multiple unique amino acid mutations were identified in the ORF1ab, HE, S, M, and N proteins. Notably, molecular docking analysis suggests that mutations S158F and L161F in the HE lectin domain are associated with improved docking scores, indicating a potential increase in receptor-binding affinity. Consecutive nucleotide deletions in two non-coding regions between non-structural protein genes-which were also identified in strains of a Thai lineage (OQ621707.1-OQ621727.1)-were observed. A CRCoV strain (106 TCID50/mL) was isolated, and experimental infection confirmed its ability to induce pneumonia and tracheal cilia loss in dogs. These findings reveal the emergence and unique genetic diversity of a novel CRCoV variant in China, highlighting the need for ongoing epidemiological surveillance.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"2574506"},"PeriodicalIF":5.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12536626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145310113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of administering cardiac medication to small-breed dogs with preclinical myxomatous mitral valve disease on survival after congestive heart failure onset. 对患有二尖瓣黏液瘤病的小型犬进行心脏药物治疗对充血性心力衰竭发作后存活的影响。

IF 5.2 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-09-21 DOI: 10.1080/01652176.2025.2560412

Sin-Wook Park, Keon Kim, Yoon-Jung Do, Jong-Won Lee, Woong-Bin Ro, Chang-Min Lee

{"title":"Impact of administering cardiac medication to small-breed dogs with preclinical myxomatous mitral valve disease on survival after congestive heart failure onset.","authors":"Sin-Wook Park, Keon Kim, Yoon-Jung Do, Jong-Won Lee, Woong-Bin Ro, Chang-Min Lee","doi":"10.1080/01652176.2025.2560412","DOIUrl":"10.1080/01652176.2025.2560412","url":null,"abstract":"Myxomatous mitral valve disease (MMVD) is the most common cardiovascular disease in small-breed dogs, and some affected dogs develop congestive heart failure (CHF). Although pimobendan is recommended to delay the onset of CHF, its effect on survival following CHF onset development remains unclear. This retrospective study evaluated the survival prognosis of 143 small-breed dogs diagnosed with first-time CHF due to MMVD, comparing pretreated (n = 54) and untreated (n = 89) groups. Pretreated dogs received cardiac medications including pimobendan for at least five weeks before CHF onset. Pretreated dogs had a significantly larger normalized left ventricular internal diameter (LVIDDN; p = 0.002) and higher left atrium-to-aortic root ratio (LA/Ao; p = 0.044) at CHF onset than untreated dogs. The median survival time after CHF onset was significantly longer in untreated dogs (481 days, 95% confidence interval (CI) 393-569 days) than in pretreated dogs (212 days, 95% CI 73-351 days; p = 0.028). Univariable Cox proportional hazards analysis identified pretreatment (p = 0.031), chordae tendineae rupture (p = 0.011), and the LA/Ao (p < 0.001) as significant predictors of survival. Our findings suggest that the administration of cardiac medications, including pimobendan, prior to the onset of CHF was not independently associated with improved survival following CHF.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"2560412"},"PeriodicalIF":5.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12451951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glucose-6-phosphatase catalytic subunit 3 enhances host resistance to porcine epidemic diarrhea virus through regulating glycolysis/gluconeogenesis. 葡萄糖-6-磷酸酶催化亚基3通过调节糖酵解/糖异生增强宿主对猪流行性腹泻病毒的抗性。

IF 5.2 2区农林科学

Veterinary Quarterly Pub Date : 2025-12-01 Epub Date: 2025-10-13 DOI: 10.1080/01652176.2025.2570471

Huihui Li, Xiaoyu Huang, Na Yuan, Lixian Wang, Lijun Shi

{"title":"Glucose-6-phosphatase catalytic subunit 3 enhances host resistance to porcine epidemic diarrhea virus through regulating glycolysis/gluconeogenesis.","authors":"Huihui Li, Xiaoyu Huang, Na Yuan, Lixian Wang, Lijun Shi","doi":"10.1080/01652176.2025.2570471","DOIUrl":"10.1080/01652176.2025.2570471","url":null,"abstract":"Porcine epidemic diarrhea virus (PEDV) causes a highly contagious disease in pigs, and the intricacies of its host-pathogen interactions require further elucidation. Chinese Min piglets, known for their superior resistance to stress and disease, were compared with Yorkshire piglets to investigate breed-specific resistance mechanisms. We established PEDV infection models in both breeds and analyzed differences by assessing cytokine levels, viral loads, and histological changes in jejunal tissues. Transcriptomic analysis of jejunal tissues identified 5422 differentially expressed (DE) protein-coding genes (PCGs) and 1999 DE long non-coding RNAs (lncRNAs) between the two pig breeds. Functional annotation revealed that Yorkshire piglets exhibited upregulation of inflammatory and apoptotic pathways, whereas Chinese Min piglets displayed strong inflammatory responses and enhanced mucosal immunity. Notably, glucose-6-phosphatase catalytic subunit 3 (G6PC3) expression was significantly higher in Chinese Min piglets than in Yorkshire piglets. Knockdown of G6PC3 in the intestinal porcine epithelial cell line J2 (IPEC-J2) resulted in increased PEDV replication and decreased expression of immune-related genes involved in the glycolysis/gluconeogenesis metabolism pathway. These findings highlight the distinct immune responses of Chinese Min and Yorkshire piglets to PEDV infection, and identify key PCGs and lncRNAs associated with PEDV immunity.","PeriodicalId":51207,"journal":{"name":"Veterinary Quarterly","volume":"45 1","pages":"2570471"},"PeriodicalIF":5.2,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12519583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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