Turkish Journal of Gastroenterology最新文献_第3页

hsa_circ_0007376 Promotes Gastric Cancer Proliferation and Malignant Metastasis by Enhancing the Stability of IGF2BP3. hsa_circ_0007376通过增强IGF2BP3的稳定性促进胃癌增殖和恶性转移。

IF 1.6 4区医学

Turkish Journal of Gastroenterology Pub Date : 2025-05-20 DOI: 10.5152/tjg.2025.24491

LinHu Liang, Ting Han, ZhengRong Zhang, ZhengWu Cheng, HaoRan Li

{"title":"hsa_circ_0007376 Promotes Gastric Cancer Proliferation and Malignant Metastasis by Enhancing the Stability of IGF2BP3.","authors":"LinHu Liang, Ting Han, ZhengRong Zhang, ZhengWu Cheng, HaoRan Li","doi":"10.5152/tjg.2025.24491","DOIUrl":"10.5152/tjg.2025.24491","url":null,"abstract":"Background/Aims: This study investigated the action of hsa_circ_0007376 in promoting the proliferation and metastasis of gastric cancer (GC). Materials and Methods: hsa_circ_0007376 was detected in GC tissues and cells by quantitative reverse transcription polymerase chain reaction. RNase R digestion, nucleoplasmic separation, and actinomycin D assays were conducted to detect the presence of hsa_circ_0007376 and its cyclic nature. The tumor-promoting effect of hsa_circ_0007376 in GC cells was verified by CCK-8, colony formation, wound healing, and Transwell assays. An interplay between hsa_circ_0007376 and insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) was confirmed by FISH, RIP, and RNA pull-down experiments. The function of hsa_circ_0007376 on GC proliferation and metastasis was evaluated in vivo in a GC xenograft mouse model. Results: hsa_circ_0007376 was highly expressed in GC. hsa_circ_0007376 was associated with lymphatic metastasis, Tumor node metastasis (TNM) stage, and tumor size in GC. When hsa_circ_0007376 was knocked down, GC cells were prevented from proliferating, migrating, and invading, as well as being prevented from metastasizing. hsa_circ_0007376 was able to bind to IGF2BP3, thereby promoting GC. Conclusion: hsa_circ_0007376 may play a role in GC by interacting and enhancing the stability of the IGF2BP3 protein.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"1 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

lncRNA FENDRR Predicts Adverse Prognosis and Regulates the Development of Esophageal Squamous Cell Carcinoma Through Negatively Modulating miR-495-3p. lncRNA FENDRR通过负调控miR-495-3p预测食管鳞状细胞癌的不良预后并调控其发展。

IF 1.6 4区医学

Turkish Journal of Gastroenterology Pub Date : 2025-05-20 DOI: 10.5152/tjg.2025.24350

Yangyang Xue, Ran Yang, Ping Gong, Hongjuan Zhu

{"title":"lncRNA FENDRR Predicts Adverse Prognosis and Regulates the Development of Esophageal Squamous Cell Carcinoma Through Negatively Modulating miR-495-3p.","authors":"Yangyang Xue, Ran Yang, Ping Gong, Hongjuan Zhu","doi":"10.5152/tjg.2025.24350","DOIUrl":"10.5152/tjg.2025.24350","url":null,"abstract":"Background/Aims: Esophageal squamous cell carcinoma (ESCC) is a major subtype of esophageal carcinoma and is highly prevalent in China. Identification of effective biomarkers could benefit ESCC management and therefore improve clinical outcomes. Evaluating the expression and significance of long non-coding RNA Fetal-lethal non-coding developmental regulatory RNA (FENDRR) in ESCC aims to provide a biomarker candidate for ESCC. Materials and Methods: This study enrolled 117 ESCC patients and collected tissue samples. The expression of FENDRR in collected samples was analyzed by polymerase chain reaction. The Chi-square, Kaplan-Meier, and Cox analyses were performed to reveal its clinical value. In ESCC cells, FENDRR was regulated by cell transfection, and its effect on cell growth and motility was evaluated. Results: FENDRR was downregulated in ESCC and was associated with large tumor size, poor differentiation, late TNM stage, positive lymph node metastasis, and adverse development-free survival of ESCC patients. FENDRR acted as an adverse indicator for the prognosis of ESCC patients. miR-495-3p was negatively regulated by FENDRR. Overexpressing FENDRR significantly suppressed ESCC cell growth and metastasis, while miR-495-3p reversed these effects. Conclusion: Downregulated FENDRR in ESCC predicted the malignant development and adverse prognosis of ESCC patients. FENDRR served as a tumor suppressor of ESCC by modulating miR-495-3p.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"1 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144152595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gasdermin D: A New Inflammatory Biomarker in Assessing Clinical Disease Activity in Crohn's Disease. 气皮蛋白D：一种评估克罗恩病临床疾病活动性的新的炎症生物标志物。

IF 1.6 4区医学

Turkish Journal of Gastroenterology Pub Date : 2025-05-20 DOI: 10.5152/tjg.2025.24726

Osman Cagin Buldukoglu, Serkan Ocal, Galip Egemen Atar, Besir Kaya, Muhammet Devran Isik, Gul Aydin Tigli, Serdar Akca, Ferda Akbay Harmandar, Yesim Cekin, Ayhan Hilmi Cekin

{"title":"Gasdermin D: A New Inflammatory Biomarker in Assessing Clinical Disease Activity in Crohn's Disease.","authors":"Osman Cagin Buldukoglu, Serkan Ocal, Galip Egemen Atar, Besir Kaya, Muhammet Devran Isik, Gul Aydin Tigli, Serdar Akca, Ferda Akbay Harmandar, Yesim Cekin, Ayhan Hilmi Cekin","doi":"10.5152/tjg.2025.24726","DOIUrl":"10.5152/tjg.2025.24726","url":null,"abstract":"Background/Aims: Crohn's disease (CD) is an inflammatory, progressive disorder requiring monitoring of treatment response and disease course. Gasdermin D (GSDMD), a protein belonging to the gasdermin protein family, plays a role in inflammatory cell death, and activation of GSDMD has been shown to be a component of the pathogenesis of inflammatory bowel disease. Considering the role that GSDMD plays in inflammation, it was hypothesized that disease activity in CD may be correlated with serum GSDMD levels. The aim of this study was to assess the strength of GSDMD in predicting clinical disease activity in patients with CD in a prompt and easy manner. Materials and Methods: This cross-sectional study was conducted over a span of 22 months from September 2022 to June 2024. A total of 61 patients with CD were included in the study. Demographic data, disease- and treatment-related data, and laboratory workups of the patients were recorded and analyzed. Results: Gasdermin D levels were statistically significant in their correlation with the Harvey-Bradshaw Index (HBI) scores of the study population (P = .019). A threshold value of 5 ng/mL for GSDMD had a sensitivity of 84.6% and a specificity of 91.7% in differentiating patients with remission or mild disease from those with moderate or severe disease, according to HBI. Conclusion: This pioneering study revealed that serum GSDMD can be used as a biomarker to assess clinical disease activity in patients with CD. Future studies incorporating colonoscopic evaluation into the equation will provide more insight into the use of this protein as a surrogate marker of disease progression in CD.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"1 1","pages":"556-562"},"PeriodicalIF":1.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Six-Month Outcomes of Endoscopic Intragastric Balloon Therapy: A Prospective Single-Center Study. 内镜胃内球囊治疗的六个月结果：一项前瞻性单中心研究。

IF 1.6 4区医学

Turkish Journal of Gastroenterology Pub Date : 2025-05-20 DOI: 10.5152/tjg.2025.25043

Mahmut Yüksel, Kerem Kenarlı, Fırathan Sarıaltın, Emine Sena Sözen, Ahmet Akbay, Erdoğan Deniz, Kübra Köken, Çağdaş Erdoğan, Mevlüt Hamamcı, Hasan Tankut Köseoğlu

{"title":"Six-Month Outcomes of Endoscopic Intragastric Balloon Therapy: A Prospective Single-Center Study.","authors":"Mahmut Yüksel, Kerem Kenarlı, Fırathan Sarıaltın, Emine Sena Sözen, Ahmet Akbay, Erdoğan Deniz, Kübra Köken, Çağdaş Erdoğan, Mevlüt Hamamcı, Hasan Tankut Köseoğlu","doi":"10.5152/tjg.2025.25043","DOIUrl":"10.5152/tjg.2025.25043","url":null,"abstract":"Background/Aims: Obesity is a growing global health challenge associated with increased morbidity and mortality. Intragastric balloon therapy has emerged as a minimally invasive alternative for weight management in patients unsuitable for bariatric surgery or as a bridging intervention. This study evaluates the efficacy, safety, and metabolic impacts of intragastric balloon therapy in a prospective cohort. \u0000\u0000Materials and Methods: A total of 65 patients underwent endoscopic intragastric balloon therapy between October 2023 and January 2025. Comprehensive baseline evaluations included body weight, body mass index, and computed tomography analysis of visceral and subcutaneous fat tissue thickness. Patients were followed up at 2 and 6 months post procedure. Statistical analysis was performed to assess weight loss, metabolic changes, and safety outcomes. \u0000\u0000Results: Of the initial cohort, 62 patients (mean age: 37.89 years; 75.4% female) completed the study. The median body weight decreased significantly from 104.5 kg pre-procedure to 88 kg at 6 months (P < .001). Body mass index values similarly declined, with a mean reduction from 37.59 to 32.79 kg/m2 (P < .001). Significant decreases in subcutaneous and visceral fat tissue thickness were observed (P < .001 and P = .032, respectively). Only 1 patient (1.5%) required early balloon removal due to nausea, with no other major complications reported. \u0000\u0000Conclusion: Intragastric balloon therapy is an effective and safe intervention for short-term weight loss, with significant reductions in adipose tissue. Further research is needed to evaluate its long-term efficacy and metabolic benefits.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"1 1","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total Metabolic Syndrome Score in Addition To FIB-4 Score May Be Useful in Predicting Advanced Fibrosis in Metabolic Dysfunction-Associated Steatotic Liver Disease. 除FIB-4评分外，总代谢综合征评分可能有助于预测代谢功能障碍相关脂肪变性肝病的晚期纤维化。

IF 1.6 4区医学

Turkish Journal of Gastroenterology Pub Date : 2025-05-20 DOI: 10.5152/tjg.2025.25058

Burcu Gurbuz, Bahadir Koylu, Onur Keskin

引用次数: 0

Letter to the Editor: A Rare Case of Upper Gastrointestinal Bleeding due to Amyloidosis. 致编辑：一例罕见的上消化道淀粉样变性出血病例。

IF 1.6 4区医学

Turkish Journal of Gastroenterology Pub Date : 2025-05-20 DOI: 10.5152/tjg.2025.24476

Ali Karataş, Halit Kandemir, Gülden Bilican, Mehmet Cindoruk, Tarkan Karakan

引用次数: 0

Clinical Features and Prevalence of Osteosarcopenia in Chronic Pancreatitis. 慢性胰腺炎骨骼肌减少症的临床特征及患病率。

IF 1.6 4区医学

Turkish Journal of Gastroenterology Pub Date : 2025-05-05 DOI: 10.5152/tjg.2025.24664

Hüseyin Döngelli, Göksel Bengi, Raif Can Yarol, Canan Altay, Süleyman Dolu, Nilay Danış, Ömer Selahattin Topalak, Hale Akpınar, Servet Kızıldağ, Müjde Soytürk

{"title":"Clinical Features and Prevalence of Osteosarcopenia in Chronic Pancreatitis.","authors":"Hüseyin Döngelli, Göksel Bengi, Raif Can Yarol, Canan Altay, Süleyman Dolu, Nilay Danış, Ömer Selahattin Topalak, Hale Akpınar, Servet Kızıldağ, Müjde Soytürk","doi":"10.5152/tjg.2025.24664","DOIUrl":"10.5152/tjg.2025.24664","url":null,"abstract":"Background/Aims: The prevalence of sarcopenia and osteopenia/osteoporosis is increased in chronic pancreatitis (CP). This study aims to evaluate the prevalence and related factors of osteosarcopenia in CP patients. Materials and Methods: Eighty-three CP patients were included in this cross-sectional observational study. Sarcopenia was assessed by measuring the surface area of the paravertebral muscles at the third lumbar region. Pancreatic fecal elastase (PFE) tests evaluated exocrine pancreatic insufficiency (EPI), while dual-energy x-ray absorptiometry scans assessed osteopenia/osteoporosis. EORTC PAN26 and a symptom questionnaire were administered, alongside nutritional marker assessments concurrent with PFE. Results: The prevalence of sarcopenia and osteopenia/osteoporosis was found to be 22.9% (n = 19) and 68.7% (n = 44), respectively. Factors associated with sarcopenia included male gender (OR: 4.9, P = .044), severe EPI (OR: 4.2, P = .043), smoking (OR: 4.6, P = .040), and zinc deficiency (OR: 2.2, P = .029). Severe EPI was significantly associated with osteopenia/osteoporosis (P = .010), diabetes mellitus (P = .001), sarcopenia (P = .016), and zinc deficiency (P = .012). Individuals with osteoporosis had higher PAN26 scores (P = .029). Factors independently associated with osteopenia/osteoporosis included female gender (OR: 7.8, P = .004), severe EPI (OR: 8.1, P = .003), and sarcopenia (OR: 5, P = .037). The prevalence of osteosarcopenia was 19.2%. Conclusion: Osteosarcopenia is common in CP patients. Smoking, zinc deficiency, EPI, and male gender are strongly associated with sarcopenia. Screening for osteosarcopenia is essential in CP patients to facilitate appropriate interventions.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"36 10","pages":"669-679"},"PeriodicalIF":1.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12520151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Helicobacter pylori Promoted miR-196a/b-5p Expression and Accelerated Tumorigenesis of the Gastric Mucosa by Targeting IGF2BP1 and Activating PI3K-Akt Signaling Pathway. 幽门螺杆菌通过靶向IGF2BP1和激活PI3K-Akt信号通路促进miR-196a/b-5p的表达，加速胃粘膜的肿瘤发生。

IF 1.6 4区医学

Turkish Journal of Gastroenterology Pub Date : 2025-05-05 DOI: 10.5152/tjg.2025.24397

Ke Chen, Jia-Wei Chen, Yao Shen, Yun-Fei Wang, Sheng-Rong Dong, Xiao-Xue Zhang, Chen-Yang Li, Xiao-Juan Gao, Jia-Min Zhao, Yu-Nan Zhang, Wen-Ying Tian, Jia-Le Lv, Qiang Zhan, Fang-Mei An

{"title":"Helicobacter pylori Promoted miR-196a/b-5p Expression and Accelerated Tumorigenesis of the Gastric Mucosa by Targeting IGF2BP1 and Activating PI3K-Akt Signaling Pathway.","authors":"Ke Chen, Jia-Wei Chen, Yao Shen, Yun-Fei Wang, Sheng-Rong Dong, Xiao-Xue Zhang, Chen-Yang Li, Xiao-Juan Gao, Jia-Min Zhao, Yu-Nan Zhang, Wen-Ying Tian, Jia-Le Lv, Qiang Zhan, Fang-Mei An","doi":"10.5152/tjg.2025.24397","DOIUrl":"10.5152/tjg.2025.24397","url":null,"abstract":"Background/Aims: The study focuses on examining the impact of Helicobacter pylori (H. pylori) on the modulation of the miRNA expression profiles while also unraveling the associated pathways that play a significant role in initiating and driving the development of gastric cancer (GC). Materials and Methods: An in-depth analysis of miRNA expression profiles in gastric tissue samples from patients with chronic superficial gastritis (CSG), chronic atrophic gastritis (CAG), dysplasia (Dys), or GC was conducted. The carbon-13 urea breath test was used to identify H. pylori infection, and the participant cohort was characterized by the presence of H. pylori infection. Additionally, the role of miR-196a/b-5p in GC carcinogenesis was investigated. Results: A total of five miRNAs-miR-196a-5p, 196b-5p, 224-5p, 424-3p, and 941-demonstrated marked elevation in CSG, CAG, Dys, and GC. miR-196a/b-5p was observed to be upregulated in GC cells following H. pylori infection, as well as in Dys and GC tissue samples from patients harboring H. pylori. miR-196a/b-5p can expedite GC progression. Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1), the target gene of miR-196a/b-5p, diminishes the proliferation capability of GC cells; however, miR-196a/b-5p can partially counteract this effect. miR-196a/b-5p activates the PI3K-Akt pathway, while IGF2BP1 inhibits the expression of these proteins. Conclusion: The levels of miR-196a/b-5p were observed to escalate following H. pylori infection, subsequently fostering the progression of GC by specifically targeting IGF2BP1 and triggering the PI3K-Akt signaling cascade.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"36 10","pages":"658-668"},"PeriodicalIF":1.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12520144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA SNHG4 Regulates Lipid Metabolism and Inflammation in Non-Alcoholic Fatty Liver Disease by Targeting miR-34b-5p/XIAP Axis. LncRNA SNHG4通过靶向miR-34b-5p/XIAP轴调控非酒精性脂肪肝的脂质代谢和炎症。

IF 1.6 4区医学

Turkish Journal of Gastroenterology Pub Date : 2025-05-05 DOI: 10.5152/tjg.2025.24191

YanJu Ding, Sheng Zheng, Xin Nian Fu, Chi Ma, Yang Juan

{"title":"LncRNA SNHG4 Regulates Lipid Metabolism and Inflammation in Non-Alcoholic Fatty Liver Disease by Targeting miR-34b-5p/XIAP Axis.","authors":"YanJu Ding, Sheng Zheng, Xin Nian Fu, Chi Ma, Yang Juan","doi":"10.5152/tjg.2025.24191","DOIUrl":"10.5152/tjg.2025.24191","url":null,"abstract":"Background/Aims: This research aimed at probing the mechanism of long non-coding RNA small nucleolar RNA host gene 4 (SNHG4) in regulating lipid metabolism and inflammation in non-alcoholic fatty liver disease (NAFLD). Materials and Methods: L02 and THLE-2 cells were stimulated with free fatty acids (FFA) and transfected. Lipid accumulation was detected through Oil Red O staining, measurements of triglyceride and total cholesterol were taken, and the levels of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 were assessed using enzyme-linked immunosorbent assays. The assessment of gene expression was conducted using real-time reverse transcriptase-polymerase chain reaction and Western blot techniques. The interplay between SNHG4 and miR-34b-5p/X-linked inhibitor of apoptosis protein (XIAP) was evaluated. Results: Free fatty acids downregulated SNHG4 and XIAP and upregulated miR-34b-5p in L02 and THLE-2 cells, leading to lipid metabolism disorder and inflammation. SNHG4 overexpression mitigated the lipid metabolism disorder and inflammation triggered by FFA, while this effect was suppressed by silencing XIAP. In contrast, SNHG4 knockdown aggravated FFA-induced lipid metabolic and inflammatory disorders, whilst this effect was rescued by inhibiting miR-34b-5p. Conclusion: SNHG4 regulates lipid metabolism and inflammatory disorders in NAFLD by targeting the miR-34b-5p/XIAP axis.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":"36 10","pages":"629-640"},"PeriodicalIF":1.6,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12520139/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Knockdown of USP34 Inhibits the Progression of Hepatocellular Carcinoma by Accelerating c-Myc Degradation. 敲低USP34通过加速c-Myc降解抑制肝细胞癌的进展

IF 1.6 4区医学

Turkish Journal of Gastroenterology Pub Date : 2025-04-21 DOI: 10.5152/tjg.2025.24335

Hailiang Liu, Xile Wei, Ye Nie, Jianshan Liu, Ming Fan

{"title":"The Knockdown of USP34 Inhibits the Progression of Hepatocellular Carcinoma by Accelerating c-Myc Degradation.","authors":"Hailiang Liu, Xile Wei, Ye Nie, Jianshan Liu, Ming Fan","doi":"10.5152/tjg.2025.24335","DOIUrl":"10.5152/tjg.2025.24335","url":null,"abstract":"Background/aims: The function and mechanism of ubiquitin-specific protease 34 (USP34) in hepatocellular carcinoma (HCC) were explored to provide new molecular targets for treating HCC.Materials and methods: In the present study, bioinformatics techniques, quantitative real-time polymerase chain reaction, and western blot were used to detect the level of USP34 in HCC tissues and cell lines. Small interfering RNA of USP34 was transfected into HCC cells, and then Cell Counting Kit-8 (CCK-8) assay, wound healing assay, and transwell assay were performed to verify the proliferation, migration and invasion of HCC cells. Enzyme-linked immunosorbent assay was utilized to assess the glycolysis level in HCC cells. Co-immunoprecipitation was used to evaluate the ubiquitination level of cellular Myc (c-Myc).Results: The expression of USP34 was upregulated in HCC patients and strongly associated with worse outcomes. Meanwhile, interference with USP34 suppressed the proliferation, migration, and invasion of HCC cells. In addition, silencing of USP34 reduced the glucose uptake, lactate production and ATP content in HCC cells, as well as downregulated the expression levels of glycolysis-related proteins (hexokinase 2, glucose transporter 1, pyruvate kinase M2, and lactate dehydrogenase A). Furthermore, the knockdown of USP34 enhanced the ubiquitination level of c-Myc and increased the degradation of c-Myc. Overexpression of c-Myc reverted the inhibitory effects of si-USP34 on the malignant biological behavior in HCC cells.Conclusion: The USP34 regulates aerobic glycolysis and inhibits the progression of HCC by accelerating c-Myc ubiquitinated degradation.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":" ","pages":"573-582"},"PeriodicalIF":1.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12432163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144004743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0