Turkish Journal of Gastroenterology最新文献

Circ_0008035 Promotes Gastric Cancer Development via the miR-429/SMAD2 Cascade. Circ_0008035 通过 miR-429/SMAD2 级联促进胃癌发展

IF 1.4 4区医学

Turkish Journal of Gastroenterology Pub Date : 2024-10-01 DOI: 10.5152/tjg.2024.23341

Yan Chen, Weigang Bian, Surong Chen

{"title":"Circ_0008035 Promotes Gastric Cancer Development via the miR-429/SMAD2 Cascade.","authors":"Yan Chen, Weigang Bian, Surong Chen","doi":"10.5152/tjg.2024.23341","DOIUrl":"https://doi.org/10.5152/tjg.2024.23341","url":null,"abstract":"The vital roles of circular RNAs (circRNAs) in human tumorigenesis have attracted more attention. Circ_0008035 is one of the most up-regulated circRNAs in gastric cancer (GC). Herein, we explored the associated mechanism of circ_0008035 in GC. EdU incorporation experiments were performed to monitor cell proliferation ability. Cell cycle progression, apoptosis, angiogenesis, migration, and invasion were analyzed using flow cytometry, Tube formation, and Transwell assays respectively. Protein expression was detected by Western blot. Dual-luciferase reporter experiments were applied to demonstrate the relationship between circ_0008035 or SMAD family member 2 (SMAD2) and microRNA-429 (miR-429). Mouse xenograft assays were conducted for evaluation of the role of circ_0008035 in vivo. Circ_0008035 content was elevated in GC tissues (P < .0001) and cell lines (P < .001), and its deficiency hindered GC cell proliferation (P < .01), HUVEC angiogenesis (P < .05), and GC cell metastasis (P < .01) and triggered apoptosis (P < .01). Circ_0008035 could sponge miR-429 to up-regulate SMAD2 expression (P < .0001). Circ_0008035 absence restrained tumor growth in vivo (P < .01). MiR429 was a mediator of circ_0008035 function, and miR-429 hindered GC cell malignant phenotypes by SMAD2. Circ_0008035 aggravates GC cell malignant progression partially by targeting the miR-429/SMAD2 axis. Considering the inhibitory effect of circ_0008035 deficiency on GC progression, targeting circ_0008035 may be a potential approach to prevent or treat GC.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenium Attenuates Ethanol-induced Hepatocellular Injury by Regulating Ferroptosis and Apoptosis. 硒通过调节铁凋亡和细胞凋亡减轻乙醇诱导的肝细胞损伤

IF 1.4 4区医学

Turkish Journal of Gastroenterology Pub Date : 2024-09-05 DOI: 10.5152/tjg.2024.24159

Feng Chen, Qianhui Li, Xiaomin Xu, Fei Wang

{"title":"Selenium Attenuates Ethanol-induced Hepatocellular Injury by Regulating Ferroptosis and Apoptosis.","authors":"Feng Chen, Qianhui Li, Xiaomin Xu, Fei Wang","doi":"10.5152/tjg.2024.24159","DOIUrl":"https://doi.org/10.5152/tjg.2024.24159","url":null,"abstract":"Ferroptosis is a newly identified type of cell death which is strongly linked to the development of several diseases. Whereas, the role of ferroptosis in the improvement of ethanol-induced hepatocytes injury by selenium has not been confirmed. In this study, an in vitro cell damage model was established using half inhibition concentration of ethanol to induce NCTC clone 1469. Cell activity, lipid peroxidation, apoptosis and the expression of markers related to ferroptosis pathway was determined. A mouse model of alcoholic liver disease (ALD) was constructed and the effectiveness of selenium and ferrostatin-1 in treating ALD in vivo was assessed by serum liver function tests, tissue staining and immunohistochemistry for ferroptosis related proteins. Pretreatment with selenomethionine and ebselen significantly improved ethanol-induced reduction in hepatocyte viability, elevated GSH levels and SOD enzyme activity, reduced MDA and iron content, while improving ethanol-induced changes in apoptosis levels and ferroptosis markers GPX4, SLC7A11, and ACSL4, with the effect of Selenomethionine being more significant. In vivo results also indicated that intervention with selenium or ferroptosis inhibitors significantly improved ethanol-induced liver tissue damage, significantly reduced serum ALT and AST levels, upregulated GPX4 and SLC7A11, but reduced ACSL4 protein levels in liver tissue. The process of ethanol damage to hepatocytes is regulated by the ferroptosis pathway. Selenium may exert a beneficial role in ethanol-induced hepatocyte injury by antagonizing oxidative stress and regulating apoptosis and ferroptosis pathways.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact and Mechanism of Action of Peptostreptococcus anaerobius on Chemotherapy Resistance in Human Colorectal Cancer. 厌氧肽链球菌对人类结直肠癌耐药性的影响和作用机制

IF 1.4 4区医学

Turkish Journal of Gastroenterology Pub Date : 2024-09-05 DOI: 10.5152/tjg.2024.24221

Guangcai Li, Wenwen Li, Hongsheng Dai, Xialian He, Lihong Shi, Xiaoqian Zhang

{"title":"The Impact and Mechanism of Action of Peptostreptococcus anaerobius on Chemotherapy Resistance in Human Colorectal Cancer.","authors":"Guangcai Li, Wenwen Li, Hongsheng Dai, Xialian He, Lihong Shi, Xiaoqian Zhang","doi":"10.5152/tjg.2024.24221","DOIUrl":"https://doi.org/10.5152/tjg.2024.24221","url":null,"abstract":"Peptostreptococcus anaerobius plays an important role in the development of colorectal cancer, and previous studies by our group have demonstrated that Peptostreptococcus anaerobius promotes resistance to 5-Fu chemotherapy in animal models of colorectal cancer. In this study, the effects of Peptostreptococcus anaerobius on chemotherapy resistance in colorectal cancer and its possible mechanism of action were investigated from the clinical point of view. Patients were selected according to exclusion and inclusion criteria and divided into sensitive and chemotherapy groups (n = 20/group). Fecal samples were collected from the patients. The bacterial 16S rRNA genes in the samples were sequenced and the abundance and varieties in the fecal bacteria were compared between the 2 groups. Immunohistochemistry and Western blotting were used to assess interleukin 23 levels in tumor tissues. Significantly elevated abundance of Peptostreptococcus was observed in fecal samples from chemoresistant colorectal cancer patients compared to those from chemosensitive individuals. Immunohistochemistry and Western blotting results showed that chemoresistant patients had higher levels of interleukin 23 relative to chemosensitive patients and the levels were positively associated with Peptostreptococcus. Peptostreptococcus may mediate the development of chemoresistant colorectal cancer by promoting the upregulation of interleukin 23. Efforts to target Peptostreptococcus thus have the potential to alter the prognosis of colorectal cancer patients.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beyond the Usual Suspects: Unraveling the Etiology of Abdominal Pain with Behcet's Disease Intestinal Manifestation. 超越常规疑点：揭开白塞氏病肠道表现腹痛的病因。

IF 1.4 4区医学

Turkish Journal of Gastroenterology Pub Date : 2024-09-04 DOI: 10.5152/tjg.2024.24272

Idrıs Kurt, Abdulvahap Kahveci

引用次数: 0

Esophageal Squamous Papillomatosis Associated Low-Grade Dysplasia Treated with Endoscopic Submucosal Dissection. 食管鳞状乳头状瘤病相关低度发育不良经内镜黏膜下剥离术治疗。

IF 1.4 4区医学

Turkish Journal of Gastroenterology Pub Date : 2024-09-02 DOI: 10.5152/tjg.2024.24178

Abdullah Murat Buyruk, Çağdaş Erdoğan

引用次数: 0

Endoscopic Management of Benign Gallbladder Diseases. 良性胆囊疾病的内窥镜治疗。

IF 1.4 4区医学

Turkish Journal of Gastroenterology Pub Date : 2024-09-02 DOI: 10.5152/tjg.2024.24149

Yimei Ji, Weirong Huang, Jiefang Guo, Bing Hu

引用次数: 0

lncRNA POLR2J4 Plays a Biomarker Role in Hepatitis B Virus-Related Hepatocellular Carcinoma Through Regulating miR-214-3p. lncRNA POLR2J4 通过调控 miR-214-3p 在乙型肝炎病毒相关肝细胞癌中发挥生物标记作用

IF 1.4 4区医学

Turkish Journal of Gastroenterology Pub Date : 2024-08-26 DOI: 10.5152/tjg.2024.24150

Yimei Ji, Xiaowei Chen, Xin Liu, Jianyuan Huang, Pei Liu

{"title":"lncRNA POLR2J4 Plays a Biomarker Role in Hepatitis B Virus-Related Hepatocellular Carcinoma Through Regulating miR-214-3p.","authors":"Yimei Ji, Xiaowei Chen, Xin Liu, Jianyuan Huang, Pei Liu","doi":"10.5152/tjg.2024.24150","DOIUrl":"https://doi.org/10.5152/tjg.2024.24150","url":null,"abstract":"Identifying novel therapeutic targets for hepatitis B virus (HBV)-induced hepatocellular carcinoma (HCC) has become a key goal in liver cancer research. Even though long non-coding RNAs (lncRNAs) do not code proteins, they could regulate the expression of functional genes and thus mediate disease development. The aim of this study was to estimate the role of lncRNA POLR2J4 (POLR2J4) in the progression of hepatitis B virus-related hepatocellular carcinoma (HBV-HCC) to pinpoint a potential biomarker. This study included 109 patients diagnosed with HBV-positive HCC, from whom tissue samples were collected. The expression level of POLR2J4 was evaluated by qPCR. The significance of POLR2J4 in HBV-HCC development and prognosis was estimated by Chi-square, Kaplan-Meier, and Cox analysis. In vitro, POLR2J4 was regulated in HBV-HCC cells, and its effect on cell growth and metastasis was assessed by CCK8 and Transwell assay. The interaction between POLR2J4 and miR-214-3p was evaluated through the luciferase reporter and RNA immunoprecipitation assays. In tumor tissues of HBV-HCC patients, there was an observed increase in the expression of POLR2J4. The increase was closely related with patients' presence of cirrhosis and vascular invasion, higher AFP, and advanced Edmondson grade and TNM stage. An upregulation of POLR2J4 predicted a poor prognosis for HBV-HCC patients and served as an independent indicator. In HBV-related HCC cells, silencing POLR2J4 suppressed cell proliferation, migration, and invasion. Furthermore, POLR2J4 negatively regulated miR-214-3p reversing the inhibition of cellular processes. POLR2J4 acted as a prognostic biomarker and a tumor promoter of HBV-HCC by modulating miR-214-3p.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11465190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142480141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Psychiatric Difficulties in Children with Celiac Disease and the Relationship between Adherence to Treatment and Parental Attitudes. 乳糜泻患儿的精神障碍以及坚持治疗与父母态度之间的关系。

IF 1.4 4区医学

Turkish Journal of Gastroenterology Pub Date : 2024-08-12 DOI: 10.5152/tjg.2024.23493

Didem Ayyıldız, Zeliha Demirtaş, Emine Kınacı

{"title":"Psychiatric Difficulties in Children with Celiac Disease and the Relationship between Adherence to Treatment and Parental Attitudes.","authors":"Didem Ayyıldız, Zeliha Demirtaş, Emine Kınacı","doi":"10.5152/tjg.2024.23493","DOIUrl":"10.5152/tjg.2024.23493","url":null,"abstract":"Our knowledge of the factors related to parenting styles affecting adherence to diet in children with celiac diseases (CDs) and the association between psychiatric difficulties and diet compliance is largely based on limited data. Therefore, our work aims to examine primarily coexisting psychiatric difficulties in children with CD and raising attitudes of their parents and secondarily the relationship among adherence to treatment, psychiatric difficulties, and parental attitudes. Children aged 4-12 years (n = 42) who have been followed up with the diagnosis of CD in a Paediatric Gastroenterology Outpatient Clinic were compared with those of healthy controls (n = 31). One of the parents was asked to fill out the socio-demographic information form, Diet Compliance Form (only the patient group), \"Parental Attitude Research Instrument\" (PARI) and Strengths and Difficulties Questionnaire-parent form (SDQ). The scores from \"emotional problems,\" \"peer relationship problems,\" and \"total difficulties\" areas were statistically significantly higher in the disease group than healthy controls. The average score of SDQ subscales and none of the PARI subscales differed between dietary compliance +/- groups. Significant positive correlations were detected between disease duration and PARI-overprotection/extreme motherhood (r = .421, P = .017) and PARI-strict/hard discipline (r = .368, P = .038) subscales. Clarifying the factors related to parenting that may affect patients' adherence to a gluten-free diet will contribute positively to the course of the disease and the quality of life of patients and their families.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11391222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Relationship Between DNA Mismatch Repair Status and Clinicopathologic Characteristics in Colon Cancer. 结肠癌中 DNA 错配修复状态与临床病理特征之间的关系

IF 1.4 4区医学

Turkish Journal of Gastroenterology Pub Date : 2024-08-12 DOI: 10.5152/tjg.2024.23366

Mehmet Doğan, Mehmet Kılıç, Hayriye Tatlı Doğan

{"title":"The Relationship Between DNA Mismatch Repair Status and Clinicopathologic Characteristics in Colon Cancer.","authors":"Mehmet Doğan, Mehmet Kılıç, Hayriye Tatlı Doğan","doi":"10.5152/tjg.2024.23366","DOIUrl":"10.5152/tjg.2024.23366","url":null,"abstract":"DNA mismatch repair (MMR) proteins are essential for repairing genetic mutations that occur during DNA replication. Deficiency of MMR proteins results in a phenotype called microsatellite instability (MSI), which occurs in Lynch syndrome as well as sporadic colorectal cancers (CRC), and it is associated with several clinicopathological features. We aimed to investigate the association of the loss of MMR proteins with clinicopathologic considerations in our CRC series. In this retrospective study, DNA MMR protein status in CRC is evaluated in a total of 200 colorectal resection specimens by immunohistochemistry (IHC) for MLH1, MSH2, MSH6 and PMS2 protein expression. The BRAF mutation was investigated by the real-time PCR in cases with loss of MLH1 protein expression. The relationship between MMR status and clinicopathological parameters was investigated statistically. Loss of MMR protein expression was detected in 26 of 200 CRC cases. The BRAFV600E mutation was detected in 2 of the cases with MLH1 loss and accepted as sporadic. The remaining 24 cases (12%) were identified as Lynch syndrome candidates. There were statistical differences observed regarding the presence of tumor-infiltrating lymphocytes (P < .001), Crohn's-like reaction (P = .001), expansile growth (P < .001), tumor heterogeneity (P < .001), mucinous differentiation (P < .001), and presence of metastatic lymph nodes (P = .045) between sporadic cases with preserved MMR and Lynch candidates. However, difference in the survival rates between sporadic cases and Lynch candidates was not significant. Immunohistochemical staining for MMR is a practical method for predicting MSI phenotype as well as Lynch candidates. MMR expression status was found to be associated with certain clinicopathological features some of which also have prognostic significance.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11391230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validity and Diagnostic Ability of Pancreatic Exocrine Insufficiency Questionnaire in Turkish Patients. 土耳其患者胰腺外分泌功能不全问卷的有效性和诊断能力

IF 1.4 4区医学

Turkish Journal of Gastroenterology Pub Date : 2024-08-12 DOI: 10.5152/tjg.2024.24061

Dilek Oğuz, İsmail Hakkı Kalkan, Müjde Soytürk, Kadir Demir, Nevin Oruç, Göksel Bengi, Özlem Gül, Nalan Gülşen Ünal, Aslı Çiftibaşı Örmeci

{"title":"Validity and Diagnostic Ability of Pancreatic Exocrine Insufficiency Questionnaire in Turkish Patients.","authors":"Dilek Oğuz, İsmail Hakkı Kalkan, Müjde Soytürk, Kadir Demir, Nevin Oruç, Göksel Bengi, Özlem Gül, Nalan Gülşen Ünal, Aslı Çiftibaşı Örmeci","doi":"10.5152/tjg.2024.24061","DOIUrl":"10.5152/tjg.2024.24061","url":null,"abstract":"Pancreatic exocrine insufficiency (PEI) is a prevalent disease that is often underdiagnosed and undertreated, leading to resulting in diminished health-related quality of life. The PEI questionnaire (PEI-Q), a patient-reported outcome questionnaire developed to diagnose and evaluate PEI, is available only in English. The study aimed to provide a Turkish translation of PEI-Q and validate its reliability and diagnostic performance in a Turkish-speaking population with PEI. This study included 161 participants: 98 patients with PEI and 63 healthy controls. Participants underwent the PEI-Q test, and the results were statistically analyzed for reliability and validity. The diagnostic value of PEI-Q was determined using receiver operating characteristic (ROC) curves. Cronbach's alpha was used to assess internal consistency, while exploratory factor analysis was performed to determine construct validity and reveal the factor structure. The mean age of participants was 45.0 years, and 60.2% were male. Participants with PEI were significantly older than those without. Scores for abdominal, bowel movement, and total symptoms were significantly higher in patients with PEI than in controls. ROC analysis revealed good diagnostic value for PEI-Q, with areas under the curve ranging from 0.798 to 0.851 for different symptom scores. Cronbach's alpha coefficients were above 0.70, indicating good internal consistency, and exploratory factor analysis supported a 4-factor structure, accounting for 68.9% of the total variance. The Turkish version of the PEI-Q is a reliable, easy-to-use, and valid screening tool for diagnosing PEI. It consistently assesses symptoms and quality of life in patients with PEI, helping to inform diagnosis and treatment.","PeriodicalId":51205,"journal":{"name":"Turkish Journal of Gastroenterology","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11391247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142331964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0