Materials Science & Engineering C-Materials for Biological Applications最新文献

{"title":"Trifunctional nanoparticles accelerate diabetic wounds healing via oxidative-immune-vascular coordination","authors":"Yanming Zuo ,&nbsp;Qin Cao ,&nbsp;Zhihao Jin ,&nbsp;Zhiyi Feng ,&nbsp;Minhui Liang ,&nbsp;Zhiqiang Cao ,&nbsp;Haiyang Xia ,&nbsp;Huixing Lin ,&nbsp;Xiaokun Li ,&nbsp;Zhouguang Wang","doi":"10.1016/j.bioadv.2025.214682","DOIUrl":"10.1016/j.bioadv.2025.214682","url":null,"abstract":"<div><div>Current treatments for diabetic wounds remain a critical clinical challenge due to their suboptimal therapeutic efficacy. Patients with diabetic wounds suffer from prolonged inflammation, ROS overproduction, and impaired angiogenesis, creating a self-perpetuating healing cycle. Here, we report a biomimetic trifunctional nanoparticle comprising nanozymes (Heme@BSA), a ROS-scavenging core, a pro-angiogenic bFGF payload, and a pH-responsive H₂S-eluting MnS shell. This design enables spatiotemporal synergism to mitigate the self-perpetuating healing cycle in diabetic wounds, via converting ROS into O₂; microenvironment- triggered H₂S release to ameliorate excessive inflammation; and sustained bFGF delivery to promote cell proliferation and migration, facilitate well-organized collagen realignment, and expedite epithelialization. Comprehensive mechanistic analyses reveal that nanozyme- transmuted O₂ reduces HIF-1α-stabilized bFGF-mediated revascularization; H₂S-driven Nrf-2 activation and HO-1 upregulation effectively assist the nanozyme in overcoming the acute ROS microenvironment via robust antioxidant and anti-apoptotic capabilities; and bFGF-supported proliferation effectively amplifies H₂S-initiated macrophage polarization in the wound core. Collectively, our investigation offers an oxidative-immune-vascular coordinated approach for diabetic wound repair, highlighting its translational potential in the management of diabetic wounds.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214682"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146013344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multilayered electrospun membranes incorporating microspheres embedded nanofibers for enhanced wound healing","authors":"Fen Ao ,&nbsp;Wen Shen ,&nbsp;Xuemei Ge ,&nbsp;Yan Zheng","doi":"10.1016/j.bioadv.2026.214727","DOIUrl":"10.1016/j.bioadv.2026.214727","url":null,"abstract":"<div><div>Multilayer membranes that coordinate the release of different drugs to match the various stages of wound healing while managing excessive tissue exudate represent a promising therapeutic approach. However, achieving precise control over the dual-drug release and effective absorption of excess tissue exudate remains a significant clinical challenge. This study employed a “nano-in-nano” microsphere-embedded fiber strategy to construct a multilayered dual drug delivery system (ML-DDS) via sequential electrospinning. The system comprises three layers: an outer fiber layer loaded with the antibacterial drug amikacin (Am), an intermediate layer of microspheres loaded with the anti-inflammatory drug quercetin (Qu) embedded within the fibers and an inner hydrophobic ethyl cellulose layer. Structural characterization showed that the intermediate layer had a diameter of approximately 500 nm with embedded microspheres predominantly ranging from 2.5 to 3.5 μm, while the inner EC fibers measured about 150 nm in diameter. The system leverages interfacial capillary forces to transport exudate from the hydrophobic layer to the drug-carrying layer. The water contact angles of the innermost hydrophobic layer and the outermost hydrophilic layer decrease to 0° within 40 s and 60 s, respectively. Drug release channels were formed after the ethyl cellulose layer absorbs water and swells. The release time of Am extended from 120 min in the single layer to 24 h in the ML-DDS. Both Qu and Am were continuously released in an amorphous form within 24 h through matrix erosion. ML-DDS up-regulated the expression of antioxidant-related metabolites, maintained mitochondrial function and promoted wound healing with a higher healing rate at day 14 compared to the control, along with enhanced collagen deposition, up-regulated CD31 expression, and reduced COX-2 levels. This study demonstrates that ML-DDS effectively integrates multilayered exudate management with controlled dual-drug delivery, providing a novel treatment strategy for skin wounds with excessive tissue exudate.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214727"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146037702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosmarinic acid/Se4+ self-assembled nanoparticles for combinational therapy of triple-negative breast cancer","authors":"Chong Qiu ,&nbsp;Xinyu Zhou ,&nbsp;Qingchao Tu ,&nbsp;LinYing Zhong ,&nbsp;Yuanfeng Fu ,&nbsp;Yinan Liu ,&nbsp;Yuqing Meng ,&nbsp;Qiaoli Shi ,&nbsp;Junzhe Zhang ,&nbsp;Shuntao Liang ,&nbsp;Fei Xia ,&nbsp;Jigang Wang","doi":"10.1016/j.bioadv.2025.214694","DOIUrl":"10.1016/j.bioadv.2025.214694","url":null,"abstract":"<div><div>Triple-negative breast cancer (TNBC) presents a formidable clinical challenge due to its aggressive nature and limited treatment options, with immunotherapy often hampered by the immunosuppressive “cold” tumor microenvironment. To address this, we developed a biomimetic nanoplatform (RA-Se@M) through the self-assembly of natural rosmarinic acid (RA) and selenium ions (Se⁴⁺), followed by coating with homologous cancer cell membranes. This design leverages the direct anti-tumor activity of RA and the immunomodulatory capacity of Se⁴⁺, while the membrane cloak confers immune evasion and enhanced tumor-targeting capability. The resulting RA-Se@M nanoparticles demonstrated superior homologous targeting and accumulation in 4T1 tumors in vivo. Beyond inducing potent apoptosis in cancer cells, RA-Se@M triggered robust immunogenic cell death (ICD), as evidenced by calreticulin exposure, HMGB1 release, and dendritic cell maturation. Consequently, in a syngeneic TNBC mouse model, RA-Se@M significantly suppressed tumor growth and, when combined with anti-PD-1 therapy, promoted profound intratumoral infiltration of cytotoxic T cells and achieved the most potent therapeutic outcome. The work showcases a promising biomimetic strategy that synergizes targeted chemotherapy and in situ immunotherapy to potentiate the anti-tumor immune response against TNBC.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214694"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymer microspheres in endovascular therapy: From fundamental research to clinical application","authors":"Andrey N. Kuskov ,&nbsp;Pavel P. Kulikov ,&nbsp;Pavel A. Yudaev ,&nbsp;Anton S. Tupikov ,&nbsp;Ekaterina A. Lesovaya ,&nbsp;Anton A. Keskinov ,&nbsp;Alexander A. Artyukhov","doi":"10.1016/j.bioadv.2025.214695","DOIUrl":"10.1016/j.bioadv.2025.214695","url":null,"abstract":"<div><div>This review highlights the current state of research in the field of the use of microspheres based on natural and synthetic polymers for embolization of hepatocellular carcinoma vessels, embolization of arteries in patients with paragangliomas, uterine myoma, prostatic hyperplasia and hemorrhoids. Particular attention in the review is paid to clinical studies of various sizes of microspheres to determine the effectiveness and safety of embolization processes. Based on the analysis of works over the past five years in the field of chemoembolization and radioembolization, it can be concluded that the most promising polymer matrices for the development of microspheres are cross-linked natural polymers, such as gelatin and chitosan. It has been established that polyvinyl alcohol microspheres and liquid embolizing agents based on ethylene-vinyl alcohol copolymer are effective and safe for preoperative embolization of patients with paragangliomas of various localizations. It is shown that the most promising embolizing agents for the treatment of uterine myoma, prostate hyperplasia and internal hemorrhoids are microspheres of cross-linked trisacryl coated with gelatin and microspheres based on polymethyl methacrylate coated with polyphosphazene. Unlike previous reviews focusing on single applications, this work provides a unique cross-disciplinary analysis of polymer microsphere embolization across multiple clinical specialties, highlighting material-design-clinical outcome relationships. The review may be of interest to bioengineers, materials scientists, chemists, and physicians working in the field of biopolymer materials and interventional therapies.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214695"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145967665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Device surface-functionalized ac-SDKP nanoassemblies accelerate endothelial recovery through dual anti-inflammatory and endothelial homeostatic mechanisms","authors":"Li Li ,&nbsp;Huanran Wang ,&nbsp;Lai Wei ,&nbsp;Jinyu Su ,&nbsp;Zheng Zeng ,&nbsp;Jianying Tan ,&nbsp;Dan Zou ,&nbsp;Junying Chen","doi":"10.1016/j.bioadv.2026.214703","DOIUrl":"10.1016/j.bioadv.2026.214703","url":null,"abstract":"<div><div>While stents are cornerstone treatments for atherosclerosis, in-stent restenosis remains a challenge. Engineering stent surfaces with specific biochemical signals can prevent this by promoting the crucial and timely regeneration of the endothelium. In this work, Ac-SDKP (N-Acetyl-Ser-Asp-Lys-Pro), an anti-inflammatory and endothelial homeostasis-regulating peptide, was incorporated into a blood-compatible PAMAM (poly-amidoamine) platform to yield pro-endothelial repair nanoassemblies. Ac-SDKP nanoassemblies attenuated macrophage activity and TNF α secretion, as well as neutrophil myeloperoxidase release. Simultaneously, the modified surface enhanced endothelial cell (EC) activity, proliferation and protection. The immobilized Ac-SDKP suppressed both extrinsic (caspase 8 pathway) and intrinsic (cytochrome <em>c</em> pathway) apoptotic signal, along with caspase-1-mediated inflammatory death, while also activating PI3K pathway to exert an anti-death effect. Furthermore, the nanoassemblies also suppressed the transmission of EC dysfunction signals by inhibiting the IKKα-RELB pathway, including monocyte chemoattractant protein-1 (linked to inflammation) and tissue factor (promoting coagulation), thereby helping to protect endothelial function. Collectively, this novel modification strategy promoted endothelial regeneration, suppressed neointimal hyperplasia, and reduced inflammatory cell infiltration in Sprague Dawley rats. In summary, constructing Ac-SDKP nanoassemblies on a PAMAM framework promotes endothelial recovery through anti-inflammatory and endothelial protection mechanisms, offering a potential approach for preventing post-implantation complications.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214703"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145967680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral delivery of liraglutide using CPP/hyaluronic acid-modified mesoporous silica nanoparticles: A virus-inspired strategy for intestinal absorption","authors":"Narges Shams ,&nbsp;Mehdi Esfandyari-Manesh ,&nbsp;Mohammad Sharifzadeh ,&nbsp;Mohsen Amini ,&nbsp;Yousef Fatahi ,&nbsp;Rassoul Dinarvand","doi":"10.1016/j.bioadv.2026.214714","DOIUrl":"10.1016/j.bioadv.2026.214714","url":null,"abstract":"<div><div>Oral administration of peptide-based drugs offers improved patient compliance compared to injectable formulations. However, this route is hindered by several physiological barriers like enzymatic degradation, poor intestinal permeability, and low absorption in the gastrointestinal tract. To address these challenges, a novel virus-mimicking nanocarrier was developed for the oral delivery of liraglutide. Hollow mesoporous silica nanoparticles (HMSN) were synthesized via a co-condensation method and subsequently functionalized with a positively charged cell-penetrating peptide (CPP; KLPVM) to facilitate transcellular transport, and with negatively charged hyaluronic acid (HA) to enhance mucus penetration and enable receptor-mediated targeting. Liraglutide was efficiently encapsulated within HMSN matrix, achieving an encapsulation efficiency of 96.16% and a drug loading of 19.23%. In vitro release studies showed pH-responsive behavior, with limited release under acidic gastric conditions (7%) and sustained release at near-neutral intestinal pH (77% over 12 h). Cellular uptake studies revealed enhanced internalization of the HMSN-CPP@HA (98.1%), predominantly via caveolae-mediated endocytosis. Ex vivo intestinal permeability studies further confirmed improved transmucosal transport, with permeability increasing from 34.4% for MSNs to 97.6% for HMSN-CPP@HA. Pharmacodynamic evaluation in streptozotocin-induced diabetic rat models showed that oral administration of liraglutide-loaded virus-mimicking HMSNs resulted in a 50.98% reduction in fasting blood glucose and a 14.6% decrease in body weight over 28-day period. These outcomes were comparable to those achieved via subcutaneous liraglutide administration (62.7% and 19.2% respectively). These findings suggest that HMSN-CPP@HA preserve the biological activity of liraglutide during gastrointestinal transit, enhance intestinal absorption, and mimic viral translocation mechanisms, representing a promising platform for non-invasive oral delivery of peptide drugs.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214714"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface microstructure of PEEK scaffolds regulates osteogenic differentiation via the βPIX–RAC1–NOX1 pathway","authors":"Zijian Yang ,&nbsp;Bowen Zhang ,&nbsp;Yihao Liu ,&nbsp;Kaiting Chen ,&nbsp;Qing Zhang ,&nbsp;Changning Sun ,&nbsp;Ling Wang ,&nbsp;Dichen Li ,&nbsp;Qingchu Li ,&nbsp;Huiyu Zhao","doi":"10.1016/j.bioadv.2026.214718","DOIUrl":"10.1016/j.bioadv.2026.214718","url":null,"abstract":"<div><div>Polyetheretherketone (PEEK) is widely applied in orthopedic implants, yet its intrinsic surface bioinertness limits stable osseointegration. Although a nature solution is modifying its surface to enhance cellular adhesion, other pathways related to osseointegration also hold significant value. Here, we combined fused deposition modeling (FDM) 3D printing with a two-step poly (acrylic acid)–ethylenediamine (PAA–EDA) grafting method to engineer porous PEEK scaffolds with bioactive surface microstructures. Beyond improving hydrophilicity to optimize cellular adhesion, the modified surface activated the βPIX-mediated signaling cascade, which suppressed ITGB1–RAC1–NOX1 activity, potentially delaying bone mesenchymal stem cell (BMSC) senescence and promoting osteogenic differentiation. In vivo implantation further validated that the modified scaffolds promoted bone formation and integration. Together, this work highlights a new pathway on osseointegration for PEEK surface engineering, revealing the potential of βPIX-mediated regulation as a new direction for durable bone–implant integration.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214718"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional conductive hydrogel integrating exosome delivery and electrical stimulation for enhanced diabetic wound healing","authors":"Weibin Wang ,&nbsp;Yufeng Huang ,&nbsp;Xuehui Chen ,&nbsp;Yuxiang Lei ,&nbsp;Yunquan Zheng ,&nbsp;Xianai Shi ,&nbsp;Jianmin Yang","doi":"10.1016/j.bioadv.2026.214716","DOIUrl":"10.1016/j.bioadv.2026.214716","url":null,"abstract":"<div><div>Diabetic wound healing is severely hampered by persistent inflammation, vascular dysfunction, infection risk, and oxidative stress. To overcome these challenges, we developed a multifunctional conductive hydrogel platform (Gel@Exo-ES). This system integrates exosomes derived from adipose-derived mesenchymal stem cells into a dynamic hydrogel network formed by cross-linking quaternized chitosan-polyaniline (QCS-PANI) with oxidized dextran (ODex). The resulting hydrogel exhibits good injectability, pH-responsive degradability, high antibacterial activity and conductivity. When combined with electrical stimulation, the Gel@Exo-ES significantly enhanced the proliferation, migration, and differentiation of fibroblasts (NIH-3T3), endothelial cells (HUVECs), and macrophages (Raw 264.7) <em>in vitro</em>. This synergy is attributed to the biocompatible hydrogel matrix, electrical stimulation-activated pro-healing signaling, and exosome-mediated bioactive cue delivery. In a diabetic rat model, the Gel@Exo-ES markedly accelerated wound closure by recruiting macrophages, upregulating IL-10 to drive M2 polarization, and thereby alleviating inflammation. The treatment concurrently enhanced re-epithelialization, collagen deposition, and angiogenesis. These findings demonstrate that the combined strategy of exosome-loaded conductive hydrogel and electrical stimulation presents a highly promising therapeutic platform for diabetic wound repair.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214716"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticle-mediated Zn delivery impacts neural protein phosphatase activity","authors":"André Nadais ,&nbsp;Carolina Castro ,&nbsp;Inês Martins ,&nbsp;Diogo Trigo ,&nbsp;Cláudia Nunes ,&nbsp;Ana Gabriela Henriques ,&nbsp;Maria de Lourdes Pereira ,&nbsp;Odete A.B. da Cruz e Silva","doi":"10.1016/j.bioadv.2025.214675","DOIUrl":"10.1016/j.bioadv.2025.214675","url":null,"abstract":"<div><div>In recent years, the use of nanoparticles (NPs) in diagnosis and treatment of different disorders has been a matter of intensive research. Due to their physical and chemical properties, zinc oxide nanoparticles (ZnO NP) have been explored in a range of biological applications, including cancer and neurological diseases. Regarding the latter, while some studies report protective effects of ZnO NP in cultured cells and animal models, others indicate that these NPs have a harmful impact on the brain, such as promoting oxidative stress and cell death.</div><div>Previous results from our group have suggested beneficial effects for zinc (Zn) cations in both modulating protein aggregation and on Alzheimer's disease (AD) pathology. In this context, the effect of encapsulated Zn as a nanoparticle on protein aggregation and its influence on protein phosphorylation events associated with AD were explored. The results herein presented show that ZnO NP contributed to a decrease in protein aggregation in neuronal cells. However, these NPs were also found to decrease PP1 and PP2A activity, potentially contributing to increased phosphorylation of tau and APP, which are AD pathology hallmarks.</div><div>In conclusion, while the use of NPs as a Zn delivery system may offer benefits by reducing aggregate formation, they also appear to induce undesired molecular changes, like those observed in AD. Therefore, a holistic approach should be incorporated as we move forward in this research line, as their effects on distinct cellular processes may be dual edged.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214675"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145918976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-active biological scaffold with in situ loaded Abaloparatide and bone marrow-derived mesenchymal stem cells synergistically regulatory T cell to promote bone regeneration","authors":"Mingyu Jia ,&nbsp;Zhihong Chen ,&nbsp;Huajian Zhou ,&nbsp;Haoran Jiang ,&nbsp;Yukang Zhang ,&nbsp;Yangyang Liu ,&nbsp;Min Wu","doi":"10.1016/j.bioadv.2025.214700","DOIUrl":"10.1016/j.bioadv.2025.214700","url":null,"abstract":"<div><div>Bone defect repair remains a significant challenge faced in clinical practice. Bone tissue engineering has emerged as an effective approach for bone defect repair due to its ample donor source and remarkable repair efficacy. In this study, we combined Abaloparatide (ABL) with bone marrow-derived mesenchymal stem cells (BMSCs) and utilized methacrylated gelatin (GelMA) as a matrix to successfully develop a bio-scaffold with dual activity (ABL@GelMA/BMSCs). BMSCs regulate the bone microenvironment by secreting cytokines, which promote bone repair through a paracrine mechanism. The addition of ABL not only facilitated the osteogenic differentiation and paracrine function of BMSCs but also significantly enhanced the bone induction capability of the scaffold, as evidenced by the marked upregulation of osteogenic markers (ALP, RUNX2, OCN). Furthermore, this bio-scaffold was able to upregulate the Foxp3 gene, inducing Foxp3+ regulatory T cells, thereby modulating the local immune microenvironment to promote bone formation. After implantation of this bio-scaffold into a critical-sized cranial defect model, histological results demonstrated a higher bone mineral density (BMD), and CT reconstruction indicated good healing of the defect. In summary, this bio-scaffold exhibits a powerful capacity for bone defect repair while improving the bone microenvironment through the synergistic modulation of regulatory T cells, providing a new strategy for bone defect repair.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"182 ","pages":"Article 214700"},"PeriodicalIF":6.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}