Comparative Immunology Microbiology and Infectious Diseases最新文献

{"title":"Seroprevalence and associated risk factors of equine herpesvirus type-1/-4 in selected districts of Northwest Amhara, Ethiopia","authors":"Anmut Worku ,&nbsp;Wassie Molla ,&nbsp;Ambaye Kenubih ,&nbsp;Daniel Gizaw ,&nbsp;Ayelech Muluneh ,&nbsp;Bemrew Admassu ,&nbsp;Mebrat Ejo ,&nbsp;Gashaw Getaneh Dagnaw ,&nbsp;Abebe Belete Bitew ,&nbsp;Tewodros Fentahun ,&nbsp;Kalkidan Getnet ,&nbsp;Haileyesus Dejene ,&nbsp;Kassahun Berrie ,&nbsp;Saddam Mohammed Ibrahim ,&nbsp;Abebe Tesfaye Gessese ,&nbsp;Melkie Dagnaw Fenta ,&nbsp;Bereket Dessalegn ,&nbsp;Mastewal Birhan ,&nbsp;Mebrie Zemene Kinde","doi":"10.1016/j.cimid.2024.102155","DOIUrl":"10.1016/j.cimid.2024.102155","url":null,"abstract":"<div><p>This study aimed to estimate the prevalence, determine the distribution, and identify the epidemiological risk factors of EHV-1/-4 infections in selected districts of Northwest Amhara Region. 460 serum samples were collected from equines using multistage cluster sampling technique, and a competitive Enzyme-linked immunosorbent assay (cELISA) was performed. Various risk factors for the occurrence of EHV-1/-4 were considered. Statistical analysis was performed using R version 4.3.1. 65.9% (303) equids were tested positive for antibodies against EHV-1/-4. Based on district, the highest prevalence was recorded in Wogera (86.1%), while the lowest was in Debark (47.4%). There was a significant difference (p &lt;0.05; 95% CI: 1.1067993–3.682843) in the prevalence of EHV-1/-4 among species and donkeys are 2.019 times more likely to get an EHV infection than horses. The prevalence of EHV-1/-4 was highest in equids with the age of 3–8 years and lowest in &lt; 3 years, and the difference was statistically significant (p &lt;0.05; 95% CI: 1.9812042–6.771820). Statistically significant variation (p &lt;0.05; 95% CI: 1.1173822–2.684013) was also observed between sex of equids in which females had 1.73 times higher chance to get EHV infection than males. Higher prevalence was found in lactating equids (81.6%), followed by pregnant equids (74.6%), and dry equids (66.4%). Generally, this study indicated a high and wide distribution of EHV-1/-4 infection in the study area, which needs due attention. Devising strategies to prevent and minimize the spread and occurrence of the infection is crucial.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102155"},"PeriodicalIF":2.0,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140057396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal dynamics and genetic characterization of bovine arthropod-borne parasites in Nan Province, Thailand with molecular identification of Anaplasma platys and Trypanosoma theileri","authors":"Apinya Arnuphapprasert ,&nbsp;Yudhi Ratna Nugraheni ,&nbsp;Sarawanee Khunmanee ,&nbsp;Winai Kaewlamun ,&nbsp;Morakot Kaewthamasorn","doi":"10.1016/j.cimid.2024.102156","DOIUrl":"10.1016/j.cimid.2024.102156","url":null,"abstract":"<div><p>Virulent species or strains of hematophagous borne pathogens such as <em>Anaplasma</em> spp., <em>Babesia</em> spp., <em>Theileria</em> spp., and <em>Trypanosoma</em> spp., are lethal to susceptible animals or reduce their productivity on a global scale. Nonetheless, efforts to diagnose the causative agents and assess the genotypic profiles as well as quantify the parasite burden of aforementioned parasites across seasons remain limited. Therefore, the present investigation sought to elucidate the genotypic composition of <em>Anaplasma</em> spp., <em>Babesia</em> spp., <em>Theileria</em> spp., and <em>Trypanosoma</em> spp. The findings revealed heightened infection rates during the summer, manifesting a correlation between <em>Trypanosoma</em> spp. infection and seasonal fluctuations. Among the identified pathogens, <em>Anaplasma marginale</em> emerged as the most dominant species, while the occurrence of <em>Anaplasma platys</em> in Thai cattle was confirmed via the sequencing of the <em>groEL</em> gene. Moreover, the study successfully identified two lineages of <em>Trypanosoma theileri</em>. The findings of this investigation offer valuable insights that can inform the development of preventive strategies for vector-borne diseases, such as considering the appropriate use of insect repellent, mosquito or insect nets, or eliminating breeding places for insects in each season.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102156"},"PeriodicalIF":2.0,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140057411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular investigation of Anaplasma phagocytophilum and related strains among sheep flocks from different parts of Türkiye; with a note of phylogenetic analyses of Anaplasma phagocytophilum- like 1","authors":"Ufuk Erol ,&nbsp;Omer Faruk Sahin ,&nbsp;Osman Furkan Urhan ,&nbsp;Ahmet Duran Atas ,&nbsp;Kursat Altay","doi":"10.1016/j.cimid.2024.102154","DOIUrl":"https://doi.org/10.1016/j.cimid.2024.102154","url":null,"abstract":"<div><p><em>Anaplasma phagocytophilum</em> is a vector-borne zoonotic pathogen and can infect various vertebrate hosts, especially cattle, sheep, goats, horses, and dogs. Molecular-based studies have revealed that the agent has a high genetic diversity and closely related strains circulate in hosts. In this study, 618 sheep blood samples obtained from different geographic regions of Türkiye were researched for <em>A.</em>phagocytophilum and related strains with PCR, RFLP, and DNA sequence analyses. The DNA of these pathogens was detected in 110 (17.79%) samples. RFLP assay showed that all positive samples were infected with A.phagocytophilum-like 1, whereas A.phagocytophilum–like 2 and A.phagocytophilum were not detected. Partial parts of 16 S rRNA gene of seven randomly selected positive samples were sequenced. The phylogenetic analyses of these isolates revealed that at least two A.phagocytophilum-like 1 isolates circulate among hosts in Türkiye and around the world. <em>A.</em>phagocytophilum-related strains have been reported in molecular-based studies over the last few years, but there is a lack of data on the vector competence, epidemiology, clinical symptoms, and genetic diversity of these pathogens. Therefore, large-scale molecular studies are still needed to obtain detailed data on the above-mentioned topics.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102154"},"PeriodicalIF":2.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140030688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The infection of Cysticercus fasciolaris in natural rats (Rattus species) residing in human residence areas, Nakhon Si Thammarat, Thailand","authors":"Sunsaneeya Thaikoed ,&nbsp;Watcharapong Mitsuwan ,&nbsp;Kittipong Chaisiri ,&nbsp;Noppharat Tanthanathipchai ,&nbsp;Maria de Lourdes Pereira ,&nbsp;Alok K. Paul ,&nbsp;Veeranoot Nissapatorn ,&nbsp;Phirabhat Saengsawang","doi":"10.1016/j.cimid.2024.102152","DOIUrl":"https://doi.org/10.1016/j.cimid.2024.102152","url":null,"abstract":"<div><p><em>Cysticercus fasciolaris</em> (<em>C. fasciolaris</em>) is the larval stage of a cestode parasite named <em>Taenia taeniaeformis</em> (<em>T. taeniaeformis</em>). <em>C. fasiolaris</em> is found in small rodents, especially rats. <em>Rattus</em> species are listed as intermediate hosts of this parasite, and cats are the main definitive host of <em>C. fasiolaris.</em> The objective of this study was to study the pathological, microscopic, and molecular aspects of <em>C. fasciolaris</em> in rodents residing in human residence areas. One hundred and two rodents were trapped in human settlements and dissected for larva-containing cyst examinations in the body cavity. The larvae of <em>C. fasciolaris</em> were investigated using histopathological examination, microscopic observations under a stereomicroscope and scanning electron microscope, and molecular detection using polymerase chain reaction. The prevalence of hepatic cysts containing larvae was 8.91% (95% CI = 4.16–16.24). In addition, the older larvae also had longer micropapillae. Histopathological investigation revealed normal hepatic tissue containing larvae and a scanty fluid cyst. The cyst capsule contains mostly mononuclear cells and spindle cells in all infected rats. The molecular detection using two primer sets revealed the amplicons were similar to the clade of <em>C. fasciolaris</em>. In the future, more investigation is necessary to fully understand the parasite's molecular pathogenesis and virulent molecules, which are less obvious.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102152"},"PeriodicalIF":2.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140030687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical importance of neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio and systemic immune-inflammation index in dogs with leishmaniasis","authors":"A. Durán-Galea ,&nbsp;J.I. Cristóbal-Verdejo ,&nbsp;R. Barrera-Chacón ,&nbsp;B. Macías-García ,&nbsp;M.A. González-Solís ,&nbsp;P. Nicolás-Barceló ,&nbsp;A.B. García-Ibáñez ,&nbsp;P. Ruíz-Tapia ,&nbsp;F.J. Duque-Carrasco","doi":"10.1016/j.cimid.2024.102148","DOIUrl":"10.1016/j.cimid.2024.102148","url":null,"abstract":"<div><p>Leishmaniasis is a zoonotic disease caused by Leishmania <em>spp</em>., impacts multiple systems and organs. While hematological and biochemical profiles aren’t definitive for diagnosis, recent studies have identified the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and systemic immune-inflammation index (SII) as predictors of morbidity and mortality in critically ill human and dog patients. This study examined 100 dogs diagnosed with leishmaniasis, categorized by the International Renal Interest Society (IRIS) stages 1–4. Additionally, the dogs were divided based on whether they survived less or more than one year (L1Y and G1Y). Control group consisted of 43 dogs. The NLR increased as the disease progressed (IRIS 1–4), presenting statistically significant differences (P&lt;0.05) when compared to the control group (2,37±2,08) IRIS 3 and 4 (4,59±13,39 and 6,99±12,86, respectively), and G1Y and L1Y (3,60±4,02 and 4,87±5,82, respectively). Significant changes in SII were only evident in short-term survivors (L1Y 951,93±1402) and advanced renal disease cases (IRIS 4 stage 1073,68±1901,09). Conversely, PLR remained largely unchanged. In conclusion, these results suggest that the neutrophil-to-lymphocyte ratio (NLR) and systemic immune-inflammation index (SII) may serve as potential markers for assessing disease progression and prognosis in dogs diagnosed with leishmaniasis.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102148"},"PeriodicalIF":2.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147957124000250/pdfft?md5=84a7166ec3bc64301993faddf590c54b&pid=1-s2.0-S0147957124000250-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139951484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance and public and animal health risks associated with pathogenic Escherichia coli isolated from calves","authors":"Dircéia Aparecida da Costa Custódio ,&nbsp;Carine Rodrigues Pereira ,&nbsp;Maysa Serpa Gonçalves ,&nbsp;Anna Cecília Trolesi Reis Borges Costa ,&nbsp;Pedro Felipe Rodrigues de Oliveira ,&nbsp;Bruna Henrique Pinto da Silva ,&nbsp;Giovanna Botelho Carneiro ,&nbsp;Fernanda Morcatti Coura ,&nbsp;Andrey Pereira Lage ,&nbsp;Marcos Bryan Heinemann ,&nbsp;Geraldo M.árcio da Costa ,&nbsp;Elaine Maria Seles Dorneles","doi":"10.1016/j.cimid.2024.102149","DOIUrl":"10.1016/j.cimid.2024.102149","url":null,"abstract":"<div><p>We aimed to determine the antimicrobial susceptibility profile of pathogenic <em>Escherichia coli</em> strains isolated from fecal samples of calves and buffalo calves (2008–2013), in Minas Gerais, Brazil, as well as the frequency of O157 gene and strains carrying extended-spectrum beta-lactamases (ESBL) and mobile colistin resistance (<em>mcr</em>) genes. <em>E. coli</em> strains (n=518) were tested for susceptibility against ten antimicrobials. Tetracycline was the antimicrobial with the highest resistance rate (382/518), followed by ampicillin (321/518), sulfamethoxazole/trimethoprim (312/518), chloramphenicol (192/518), gentamicin (126/518), ciprofloxacin (148/518), cefazolin (89/518), colistin (54/518) and cefoxitin (34/518). Multidrug resistance (MDR) was observed in 381/518 isolates. No strain harbored <em>mcr</em> or O157 genes<em>,</em> whereas 19/99 were ESBL positive. The most prevalent pathotype and phylogroup were STEC and B1, respectively. Age, EHEC pathotype and resistance to aminoglycoside and cephem were significantly associated with MDR in the multivariate model. Overall, <em>E. coli</em> strains showed high rates of resistance to penicillin, tetracyclines and folate inhibitors, in addition to an alarming rate of MDR and ESBL-producing strains.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102149"},"PeriodicalIF":2.0,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139923211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High diversity, novel genotypes, and vertical transmission of hemotropic Mycoplasma in micromammals","authors":"Javier Millán ,&nbsp;Bárbara Martín-Maldonado ,&nbsp;Ruth Rodríguez-Pastor ,&nbsp;Jesús Martínez-Padilla ,&nbsp;Fernando Esperón","doi":"10.1016/j.cimid.2024.102151","DOIUrl":"10.1016/j.cimid.2024.102151","url":null,"abstract":"<div><p>Hemotropic mycoplasmas (hemoplasmas) are emerging zoonotic pathogens. Micromammals have received little attention as hosts for hemoplasmas despite their ubiquitous presence, high population abundances, and close association with humans. A PCR protocol targeting a fragment of the 16 S rRNA gene and direct sequencing in blood samples of 189 adult specimens and 35 fetuses belonging to three species of Eulipotyphla (shrews) and seven species of Rodentia, captured in three ecologically diverse habitats in North-Eastern Spain (Steppe, High Mountain, Mediterranean) yielded and occurrence of 26%, including 36% of 39 shrews and 23% of 150 rodents. Sequencing revealed the presence of 14 nucleotide sequence types (ntST) among the 56 readable sequences. In general, each ntST was associated with a given host species, although in some cases, the same ntST was sequenced in different species (chiefly rodents). Most ntST were closely related to rodent and/or bat hemoplasmas, but one was identical with <em>Mycoplasma haemocanis</em>/<em>haemofelis</em>, and others can be considered novel genotypes. High sequence diversity was detected in rodents, whereas in the white-toothed shrew (<em>Crocidura russula</em>), 9/11 sequences from two distant areas were identical. Phylogenetic and network analyses classified our sequences in different clades including hemoplasmas of rodents, carnivores, bats, and humans. Twelve of the fetuses (34.2%) of 9/12 litters (75.0%) of shrews and rodents were hemoplasma-positive, indicating frequent vertical transmission. Our study contributes to expanding our knowledge about the distribution, diversity, and transmission of hemoplasmas.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102151"},"PeriodicalIF":2.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147957124000286/pdfft?md5=3954bced5f176c884d4ed047b1a6a3e4&pid=1-s2.0-S0147957124000286-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139923214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile","authors":"Paulina Sepúlveda-García ,&nbsp;Ronald Jara ,&nbsp;Armin Mella ,&nbsp;Gustavo Monti ,&nbsp;Nivia Canales ,&nbsp;Maria Eduarda Chiaradia Furquim ,&nbsp;Marcos Rogério André ,&nbsp;Ananda Müller","doi":"10.1016/j.cimid.2024.102150","DOIUrl":"10.1016/j.cimid.2024.102150","url":null,"abstract":"<div><p>The study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. ‘Seventy-nine cats’ blood DNA samples qPCR <em>Bartonella</em> spp. positive were subjected to T-A cloning of <em>Bartonella</em> spp. <em>rpoB</em> partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%–100% identity with <em>Bartonella henselae</em> whereas 9 showed 99.8–100% identity with <em>Bartonella koehlerae</em>. Haplotype analysis yielded 29 different <em>rpoB</em>-<em>B. henselae</em> haplotypes, one (hap#2) overrepresented in 31 out of 33 cats, and 4 <em>rpoB-B. koehlerae</em> haplotypes, with hap#2 represented in all 3 <em>B. koehlerae</em> infected cats. More than one <em>rpoB</em> -<em>B. henselae</em> and <em>B. koehlerae</em> haplotypes were identified in individual cats, reporting by first time coinfection by different <em>B. henselae/B. koehlerae rpoB</em> variants in cats from Chile.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102150"},"PeriodicalIF":2.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139923372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and potential risk factors for Cryptosporidium spp. infection in horses from Egypt","authors":"Mohammed H. Alruhaili ,&nbsp;Mohamed Marzok ,&nbsp;Hattan S. Gattan ,&nbsp;Mohamed Salem ,&nbsp;Mahmoud Kandeel ,&nbsp;Abdelfattah Selim","doi":"10.1016/j.cimid.2024.102140","DOIUrl":"https://doi.org/10.1016/j.cimid.2024.102140","url":null,"abstract":"<div><p><em>Cryptosporidium</em> is an intestinal protozoan that cause diarrhea in livestock all over the world and have zoonotic importance. The present study aimed to determine the prevalence of <em>Cryptosporidium</em> spp. in horses in Egypt and evaluate the associated risk factors. A total of 420 fecal samples were collected from three governorates (Giza, Kafr ElSheikh and Qalyubia) and examined microscopically using Ziehl-Neelsen staining method. The overall prevalence of <em>Cryptosporidium</em> spp. was 29% and Kafr ElSheikh governorate had the highest rate in comparison to other areas. The prevalence of <em>Cryptosporidium</em> spp. in examined horses had significant association with sex, age, type of management, absence of bedding and presence of dogs. The higher prevalence rate was observed in females (32.2%), age group less than two years (43.2%), mixed (grazing and stable) horses (36.1%), animals had history of diarrhea (33%), absence of bedding (35.1%) and contact with dogs (35.7%). These findings give baseline data for further research. It is necessary to establish control strategy for <em>Cryptosporidium</em> spp. infection in order to lower the risk of infection in animals and human.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102140"},"PeriodicalIF":2.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139726963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of anti-Leptospira spp. antibodies and leptospiruria in cats attended to a veterinary teaching hospital in southern Brazil","authors":"Mirela Grünwalder Paim ,&nbsp;Bárbara Basseggio Rivas ,&nbsp;Grasiele Amorim Sebastião ,&nbsp;Kauana Kaefer ,&nbsp;Rogério Oliveira Rodrigues ,&nbsp;Fabiana Quoos Mayer ,&nbsp;Luciana Neves Nunes ,&nbsp;Fernanda Vieira Amorim da Costa","doi":"10.1016/j.cimid.2024.102138","DOIUrl":"10.1016/j.cimid.2024.102138","url":null,"abstract":"<div><p>Leptospirosis is a bacterial zoonosis that affects both humans and animals worldwide. Currently, it is known that cats may be susceptible to infection. This study aims to investigate the presence of anti-<em>Leptospira</em> spp. antibodies and leptospiruria in cats, using Microscopic Agglutination Test (MAT) and Real-time Polymerase Chain Reaction (PCR) techniques, respectively. A total of 76 cats, undergoing comprehensive anamnesis, general physical examination, and complementary exams were included in the investigation. Among the 76 cats tested, 9.2% (7/76) exhibited the presence of anti-<em>Leptospira</em> spp. antibodies, while <em>Leptospira</em> spp. DNA was detected in at 1.3% (1/76) of the evaluated urine samples. No significant associations were observed between the serological and molecular diagnostic results and the assessed variables, including clinical data and laboratory results of cats testing positive. This study provides insight into the occurrence of <em>Leptospira</em> spp. infection and leptospiruria in cats treated at a veterinary teaching hospital in southern Brazil.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102138"},"PeriodicalIF":2.0,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139666584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}