Comparative Immunology Microbiology and Infectious Diseases最新文献

{"title":"Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile","authors":"Paulina Sepúlveda-García ,&nbsp;Ronald Jara ,&nbsp;Armin Mella ,&nbsp;Gustavo Monti ,&nbsp;Nivia Canales ,&nbsp;Maria Eduarda Chiaradia Furquim ,&nbsp;Marcos Rogério André ,&nbsp;Ananda Müller","doi":"10.1016/j.cimid.2024.102150","DOIUrl":"10.1016/j.cimid.2024.102150","url":null,"abstract":"<div><p>The study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. ‘Seventy-nine cats’ blood DNA samples qPCR <em>Bartonella</em> spp. positive were subjected to T-A cloning of <em>Bartonella</em> spp. <em>rpoB</em> partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%–100% identity with <em>Bartonella henselae</em> whereas 9 showed 99.8–100% identity with <em>Bartonella koehlerae</em>. Haplotype analysis yielded 29 different <em>rpoB</em>-<em>B. henselae</em> haplotypes, one (hap#2) overrepresented in 31 out of 33 cats, and 4 <em>rpoB-B. koehlerae</em> haplotypes, with hap#2 represented in all 3 <em>B. koehlerae</em> infected cats. More than one <em>rpoB</em> -<em>B. henselae</em> and <em>B. koehlerae</em> haplotypes were identified in individual cats, reporting by first time coinfection by different <em>B. henselae/B. koehlerae rpoB</em> variants in cats from Chile.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102150"},"PeriodicalIF":2.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139923372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and potential risk factors for Cryptosporidium spp. infection in horses from Egypt","authors":"Mohammed H. Alruhaili ,&nbsp;Mohamed Marzok ,&nbsp;Hattan S. Gattan ,&nbsp;Mohamed Salem ,&nbsp;Mahmoud Kandeel ,&nbsp;Abdelfattah Selim","doi":"10.1016/j.cimid.2024.102140","DOIUrl":"https://doi.org/10.1016/j.cimid.2024.102140","url":null,"abstract":"<div><p><em>Cryptosporidium</em> is an intestinal protozoan that cause diarrhea in livestock all over the world and have zoonotic importance. The present study aimed to determine the prevalence of <em>Cryptosporidium</em> spp. in horses in Egypt and evaluate the associated risk factors. A total of 420 fecal samples were collected from three governorates (Giza, Kafr ElSheikh and Qalyubia) and examined microscopically using Ziehl-Neelsen staining method. The overall prevalence of <em>Cryptosporidium</em> spp. was 29% and Kafr ElSheikh governorate had the highest rate in comparison to other areas. The prevalence of <em>Cryptosporidium</em> spp. in examined horses had significant association with sex, age, type of management, absence of bedding and presence of dogs. The higher prevalence rate was observed in females (32.2%), age group less than two years (43.2%), mixed (grazing and stable) horses (36.1%), animals had history of diarrhea (33%), absence of bedding (35.1%) and contact with dogs (35.7%). These findings give baseline data for further research. It is necessary to establish control strategy for <em>Cryptosporidium</em> spp. infection in order to lower the risk of infection in animals and human.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102140"},"PeriodicalIF":2.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139726963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of anti-Leptospira spp. antibodies and leptospiruria in cats attended to a veterinary teaching hospital in southern Brazil","authors":"Mirela Grünwalder Paim ,&nbsp;Bárbara Basseggio Rivas ,&nbsp;Grasiele Amorim Sebastião ,&nbsp;Kauana Kaefer ,&nbsp;Rogério Oliveira Rodrigues ,&nbsp;Fabiana Quoos Mayer ,&nbsp;Luciana Neves Nunes ,&nbsp;Fernanda Vieira Amorim da Costa","doi":"10.1016/j.cimid.2024.102138","DOIUrl":"10.1016/j.cimid.2024.102138","url":null,"abstract":"<div><p>Leptospirosis is a bacterial zoonosis that affects both humans and animals worldwide. Currently, it is known that cats may be susceptible to infection. This study aims to investigate the presence of anti-<em>Leptospira</em> spp. antibodies and leptospiruria in cats, using Microscopic Agglutination Test (MAT) and Real-time Polymerase Chain Reaction (PCR) techniques, respectively. A total of 76 cats, undergoing comprehensive anamnesis, general physical examination, and complementary exams were included in the investigation. Among the 76 cats tested, 9.2% (7/76) exhibited the presence of anti-<em>Leptospira</em> spp. antibodies, while <em>Leptospira</em> spp. DNA was detected in at 1.3% (1/76) of the evaluated urine samples. No significant associations were observed between the serological and molecular diagnostic results and the assessed variables, including clinical data and laboratory results of cats testing positive. This study provides insight into the occurrence of <em>Leptospira</em> spp. infection and leptospiruria in cats treated at a veterinary teaching hospital in southern Brazil.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102138"},"PeriodicalIF":2.0,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139666584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beta-lactamase genes in bacteria from food animals, retail meat, and human surveillance programs in the United States from 2002 to 2021","authors":"Md. Kaisar Rahman,&nbsp;Howard Rodriguez-Mori,&nbsp;Guy H. Loneragan,&nbsp;Babafela Awosile","doi":"10.1016/j.cimid.2024.102139","DOIUrl":"10.1016/j.cimid.2024.102139","url":null,"abstract":"<div><p>The spread of beta-lactamase-producing bacteria is a global public-health concern. This study aimed to explore the distribution of beta-lactamases reported in three sampling sources (cecal, retail meat, and human) collected as part of integrated surveillance in the United States. We retrieved and analyzed data from the United States National Antimicrobial Resistance Monitoring Systems (NARMS) from 2002 to 2021. A total of 115 beta-lactamase genes were detected in <em>E. coli</em>, <em>Salmonella enterica</em>, <em>Campylobacter</em>, <em>Shigella</em> and <em>Vibrio</em>: including 35 genes from cecal isolates, 32 genes from the retail meat isolates, and 104 genes from the human isolates. Three genes in <em>E. coli</em> (<em>bla</em>_CMY-2, <em>bla</em>_TEM-1A, and <em>bla</em>_TEM-1B), 6 genes in Salmonella enterica (<em>bla</em>_CARB-2, <em>bla</em>_CMY-2, <em>bla</em>_CTXM-65, <em>bla</em>_TEM-1A, <em>bla</em>_TEM-1B, and <em>bla</em>_HERA-3), and 2 genes in <em>Campylobacter</em> spp. (<em>bla</em>_OXA-61 and <em>bla</em>_OXA-449) have been detected across food animals (cattle, chicken, swine, and turkey) and humans over the study period. <em>bla</em>_CTXM-55 has been detected in <em>E. coli</em> isolates from the four food animal sources while <em>bla</em>_CTXM-15 and <em>bla</em>_CTXM-27 were found only in cattle and swine. In <em>Salmonella enterica</em>, <em>bla</em>_CTXM-2, <em>bla</em>_CTXM-9, <em>bla</em>_CTXM-14, <em>bla</em>_CTXM-15, <em>bla</em>_CTXM-27, <em>bla</em>_CTXM-55, and <em>bla</em>_NDM-1 were only detected among human isolates. <em>bla</em>_OXAs and <em>bla</em>_CARB were bacteria-specific and the only beta-lactamase genes detected in <em>Campylobacter</em> spp. and <em>Vibrio</em> spp respectively. The proportions of beta-lactamase genes detected varies from bacteria to bacteria. This study provided insights on the beta-lactamase genes detected in bacteria in food animals and humans in the United States. This is necessary for better understanding the molecular epidemiology of clinically important beta-lactamases in one health interface.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102139"},"PeriodicalIF":2.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139666937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular prevalence of vector borne bacterial pathogens in the blood samples of wild rodent species trapped from Saudi Arabia","authors":"Abdul Ghafar ,&nbsp;Samia Q. Alghamdi ,&nbsp;Abdullah D. Alanazi ,&nbsp;Syed Muhammad Zukhruf Qousain ,&nbsp;Maryam Ijaz ,&nbsp;Muhammad Naeem ,&nbsp;Muhammad Ali ,&nbsp;Hira Muqaddas ,&nbsp;Adil Khan ,&nbsp;Furhan Iqbal","doi":"10.1016/j.cimid.2024.102129","DOIUrl":"10.1016/j.cimid.2024.102129","url":null,"abstract":"<div><p>Order Rodentia is the most speciose among mammals and the members of this order are known to host more than 60 zoonotic diseases and rodents are a potential health threat to humans. This study was designed to report the molecular prevalence and phylogenetic evaluation of various blood borne bacterial pathogens (<em>Anaplasma ovis, Anaplasma phagocytophilum</em>, <em>Anaplasma marginale</em> and <em>Bartonella</em> spp<em>.</em>) in the blood samples of four wild rodent species [<em>Meriones rex</em> (N = 27), <em>Acomys dimidiatus</em> (N = 18), <em>Myomys yemeni</em> (N = 6) and <em>Rattus rattus</em> (N = 3)] that were trapped during August till October 2020 from Al Makhwah governorate in Saudi Arabia. Results revealed by 9/54 (16.6%) rodents amplified <em>Msp4</em> gene and 2/54 (3.7%) rodents amplified <em>rpoB</em> gene of <em>Anaplasma ovis</em> and <em>Bartonella</em> spp<em>.</em> respectively. <em>Anaplasma phagocytophilum</em> and <em>Anaplasma marginale</em> were not detected among enrolled rodent species. <em>Meriones rex</em> was the most highly infected rodent species. DNA sequencing and BLAST analysis confirmed the presence of <em>Anaplasma ovis</em> and the <em>Bartonella koehlerae</em> in rodent blood samples. Phylogenetic analysis of both pathogens showed that Saudi isolates were clustered together and were closely related to isolates that were reported from worldwide countries. Risk factor analysis revealed that prevalence of both bacterial pathogens was not restricted to a particular rodent species or a rodent sex (P &gt; 0.05). In conclusion, we are reporting for the very first time that Saudi rodents are infected with <em>Anaplasma ovis</em> and rodents can be infected with <em>Bartonella koehlerae</em>. Similar studies at large scale are recommended in all those areas of Saudi Arabia that are unexplored for the incidence and prevalence of bacterial pathogens among the rodents that are living near human dwellings in order to prevent bacterial infections in local people as well as in livestock<em>.</em></p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102129"},"PeriodicalIF":2.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139580279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular detection and MST genotyping of Coxiella burnetii in ruminants and stray dogs and cats in Northern Algeria","authors":"H. Ghaoui ,&nbsp;I. Bitam ,&nbsp;S. Zaidi ,&nbsp;N. Achour ,&nbsp;S. Zenia ,&nbsp;T. Idres ,&nbsp;P.E. Fournier","doi":"10.1016/j.cimid.2024.102126","DOIUrl":"10.1016/j.cimid.2024.102126","url":null,"abstract":"<div><p>Aiming at identifying the reservoir and contamination sources of <em>Coxiella burnetii</em> in Northern Algeria, we investigated the molecular presence of the bacterium in 599 samples (blood, placenta, liver, spleen, and uterus) collected from cattle, sheep, dogs and cats. Our qPCR results showed that <strong>15/344 (4.36%)</strong> blood samples and <strong>six/255 (2.35%)</strong> organ specimens were positive for <em>C. burnetii</em>. In cattle, <strong>three (4%)</strong> blood and liver samples were positive. In sheep, one blood <strong>(1.19%)</strong> and <strong>3 (8.57%)</strong> placenta samples were positive. At the Algiers dog pound, <strong>8 (10%)</strong> and <strong>3 (5%)</strong> blood samples were qPCR positivein dogs and cats, respectively. In addition, <strong>MST</strong> genotyping showed that <strong>MST 33</strong> was present in cattle and sheep, <strong>MST 20</strong> in cattle,and<strong>MST 21</strong> in dogs and cats.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102126"},"PeriodicalIF":2.0,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139558738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular survey of hemoplasmas and Coxiella burnetii in vampire bats from northern Brazil","authors":"Victória Valente Califre de Mello ,&nbsp;Laryssa Borges de Oliveira ,&nbsp;Taciana Fernandes Souza Barbosa Coelho ,&nbsp;Daniel Antonio Braga Lee ,&nbsp;Eliz Oliveira Franco ,&nbsp;Rosangela Zacarias Machado ,&nbsp;Marcos Rogério André","doi":"10.1016/j.cimid.2024.102127","DOIUrl":"10.1016/j.cimid.2024.102127","url":null,"abstract":"<div><p><span>In addition to zoonotic viral pathogens, bats can also harbor bacterial pathogens, including hemoplasmas (hemotropic mycoplasmas) and </span><span><em>Coxiella burnetii</em></span>. The present study aimed to investigate, using molecular techniques, the presence of hemoplasmas and <em>C. burnetii</em> in spleen samples from vampire bats in northern Brazil. For this purpose, between 2017 and 2019, spleen samples were collected from <span><em>Desmodus rotundus</em></span> (n = 228) and <em>Diaemus youngii</em> (n = 1) captured in the states of Pará (n = 207), Amazonas (n = 1), Roraima (n = 18) and Amapá (n = 3). DNA samples extracted from the bat spleen and positive in PCR for the endogenous <span><em>gapdh</em></span> gene were subjected to conventional PCR assays for the 16S rRNA, 23S rRNA and RNAse P genes from hemoplasmas and to qPCR based on the <em>IS1111</em> gene element for <em>C. burnetii</em>. All spleen samples from vampire bats were negative in the qPCR for <em>C. burnetii</em><span>. Hemoplasmas were detected in 10 % (23/229) of spleen samples using a PCR based on the 16S rRNA gene. Of these, 21.73 % (5/23) were positive for the 23S rRNA gene and none for the RNAseP gene. The seven hemoplasma 16S rRNA sequences obtained were closely related to sequences previously identified in vampire bats from Belize, Peru and Brazil. The 23S rRNA sequence obtained revealed genetic proximity to hemoplasmas from non-hematophagous bats from Brazil and Belize. The analysis revealed different circulating genotypes among Brazilian vampire bats, in addition to a trend towards genera-specific hemoplasma genotypes. The present study contributes to the knowledge of the wide diversity of hemoplasmas in vampire bats.</span></p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102127"},"PeriodicalIF":2.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139510283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidences of Helicobacter infection in pigs and tracing occupational hazard in pig farmers","authors":"Seema Rani Pegu ,&nbsp;Joyshikh Sonowal ,&nbsp;Swaraj Rajkhowa ,&nbsp;Pranab Jyoti Das ,&nbsp;Gyanendra Singh Sengar ,&nbsp;Rajib Deb ,&nbsp;Manjisa Choudhury ,&nbsp;Naba Jyoti Deka ,&nbsp;Souvik Paul ,&nbsp;Juwar Doley ,&nbsp;Dilip Kumar Sarma ,&nbsp;Samir Das ,&nbsp;N.H. Mohan ,&nbsp;Rajendran Thomas ,&nbsp;Vivek Kumar Gupta","doi":"10.1016/j.cimid.2024.102128","DOIUrl":"10.1016/j.cimid.2024.102128","url":null,"abstract":"<div><p><span><em>Helicobacter</em><em> species</em></span> (spp<em>.</em><span>) is a gram-negative spiral-shaped motile bacterium that causes gastritis in pigs and also colonizes in the human stomach. The present study assessed the prevalence of </span><em>Helicobacter</em><span><span> spp. in pig gastric mucosa and the stool of pig farmers in Assam, India. A total of 403 stomach samples from pig slaughter points, 74 </span>necropsy<span> samples of pigs from pig farms, and 97 stool samples from pig farmers were collected. Among the pig stomach samples, 43 (20.09%) of those with gastritis showed the presence of Gram-negative, spiral-shaped organisms, while only 3.04% of stomach samples without lesions had these organisms. Scanning Electron Microscopy (SEM) of urease-positive stomach samples revealed tightly coiled </span></span><em>Helicobacter</em><span> bacteria in the mucus lining. Histopathological examination showed chronic gastritis with hemorrhagic necrosis, leucocytic infiltration<span>, and lymphoid aggregates. PCR confirmed the presence of </span></span><em>Helicobacter suis</em> in 19.63% of pig stomach samples and 2.08% of pig farmer stool samples. Additionally, 3.12% of the stool samples from pig farmers were positive for <span><em>Helicobacter </em><em>pylori</em></span><span>. Phylogenetic analysis revealed distinct clusters of </span><em>Helicobacter suis</em> with other <em>Helicobacter</em> spp. These findings highlight the prevalence of <em>Helicobacter</em> in both pig gastric mucosa and pig farmer stool. The findings highlight the need for improved sanitation and hygiene practices among pig farmers to minimize the risk of <em>Helicobacter</em> infection in humans.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102128"},"PeriodicalIF":2.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139470469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative molecular epidemiology, subtype distribution, and zoonotic potential of Blastocystis sp. in Equus animals (horses, donkeys, and mules) in northwestern Iran","authors":"Ali Asghari ,&nbsp;Amirhosein Yousefi ,&nbsp;Mohammad Reza Mohammadi ,&nbsp;Roya Badali ,&nbsp;Laya Shamsi ,&nbsp;Ahmet Efe Köseoğlu ,&nbsp;Amir Abbaszadeh ,&nbsp;Morteza Shams ,&nbsp;Behnam Mohammadi-Ghalehbin","doi":"10.1016/j.cimid.2024.102124","DOIUrl":"10.1016/j.cimid.2024.102124","url":null,"abstract":"<div><p>A total of 500 fecal samples were collected from Equus animals in six different cities (Ardabil, Namin, Nir, Meshginshahr, Germi, and Khalkhal) of Ardabil Province, northwestern Iran, with 200 samples from horses, 200 from donkeys, and 100 from mules. Of the horse samples, 100 were from racing horses under special monitoring and care, while the remaining 100 were from non-racing horses, including those used for herding or in rural areas. All fecal samples were examined for the presence of <em>Blastocystis</em><span><span> sp. using PCR amplification of the SSU rRNA gene's barcode region after </span>DNA extraction. The molecular prevalence of </span><em>Blastocystis</em> infection in Equus animals was 7.6% (38/500). <em>Blastocystis</em> was more common in horses [11.5% (23/200)] than in donkeys [5.5% (11/200)] and mules [4% (4/100)] (P &gt; 0.05). Compared to racing horses [3% (3/100)], non-racing/rural horses [20% (20/100)] exhibited a substantially higher prevalence of <em>Blastocystis</em> (P &lt; 0.05). The prevalence of <em>Blastocystis</em> in diarrheal samples and younger animals was remarkably higher than in formed samples and older animals, respectively (P &lt; 0.05). No significant difference in <em>Blastocystis</em> infection prevalence was found between the genders of examined animals (P &gt; 0.05). In Equus animals, 38 <em>Blastocystis</em> isolates included eight STs: ST10 [31.6% (12/38)], ST1 [21.1% (8/38)], ST2 [15.8% (6/38)], ST3 [10.5% (4/38)], ST4 [7.9% (3/38)], ST7 [5.2% (2/38)], ST14 [5.2% (2/38)], and ST6 [2.6% (1/38)]. These results suggest that Equus animals act as a proper reservoir for numerous <em>Blastocystis</em><span> STs, consequently playing a crucial part in the spread of this protozoan<span> infection to humans, animals, and water reservoirs.</span></span></p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102124"},"PeriodicalIF":2.0,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139375724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic detection and phylogenetic analysis of Bartonella quintana in pet cats from Urmia City, Northwest Iran","authors":"Sima Alempour Rajabi ,&nbsp;Abdolghaffar Ownagh ,&nbsp;Mojtaba Hadian","doi":"10.1016/j.cimid.2024.102125","DOIUrl":"10.1016/j.cimid.2024.102125","url":null,"abstract":"<div><p>The aim of this study was to investigate the presence and genetic characteristics of <span><em>Bartonella quintana</em></span><span> in pet cats from Urmia City, located in the northwest of Iran. Blood samples were collected from 200 cats, and their age, gender, and breed were noted. Nested-PCR and sequencing were used to identify </span><em>B. quintana</em> in positive samples, and the <em>ftsZ</em><span> gene sequences were analyzed using BioEdit software. The gene sequence obtained in this study exhibited 100.00 % similarity to reference sequences in the GenBank® database, and a phylogenetic tree was constructed using MEGA11. The results revealed that 15 % of the cats (30 out of 200 blood samples) tested positive for the </span><em>B. quintana</em> gene, with a 95 % confidence interval of 10.71 % to 20.61 %.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"105 ","pages":"Article 102125"},"PeriodicalIF":2.0,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139375697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}