Comparative assessment of brucellosis detection in dogs: In-house ELISA versus Rose Bengal Plate Test utilizing rough and smooth antigens

IF 2 3区农林科学 Q4 IMMUNOLOGY

Comparative Immunology Microbiology and Infectious Diseases Pub Date : 2024-11-17 DOI:10.1016/j.cimid.2024.102277

Kadir Akar , Ayfer Güllü Yücetepe , İsmail Hakkı Ekin , Maryam Dadar , Sevil Erdenliğ Gürbilek

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引用次数: 0

Abstract

Brucella canis and smooth Brucella species infections have been reported in dogs globally. In endemic countries such as Türkiye, dogs can be infected with both species. The exact incidence of canine brucellosis in Türkiye is unknown. This study aims to investigate the seroprevalence of brucellosis in stray dogs undergoing sterilization in Van province, eastern Türkiye. In this study, a comprehensive approach was used to ensure reliable incidence of Brucella spp in the dogs. We used the Rose Bengal Plate Test (RBPT) with both antigens from smooth Brucella abortus S99 (RBPT-S) and rough B. canis (RBPT-R) Brucella species to analyze 150 dog blood serum samples. For the in-house enzyme-linked immunosorbent assay (i-ELISA), we used a rough Lipopolysaccharide (LPS) antigen from the rough strain (i-ELISA-R) and an O-polysaccharide (O-PS) antigen (i-ELISA-S) from the smooth strain obtained from the OIE Reference Laboratory in Weybridge, UK. Each serum sample underwent analysis using a total of four serological tests, ensuring a thorough and reliable evaluation. Out of 150 serum samples analyzed, 32 (21.3 %) tested positive using the RBPT-S test and 12 (6.6 %) with the RBPT-R test. The i-ELISA-R identified 8 (5.3 %) positive samples, while the i-ELISA-S detected 27 (18 %) positive samples. The presence of B. canis and other Brucella spp. infections was detected in the dog in this region, revealing that canine brucellosis was primarily caused by smooth Brucella species. The test beased -smooth antigens identified a significantly higher number of positive samples compared to the test beased -rough antigens test, suggesting a potential difference in sensitivity or specificity between the two methods. This situation was considered an important risk for both dogs and humans, underscoring the urgent need for further research and the immediate implementation of public health measures to address this issue.

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犬布鲁氏菌病检测的比较评估：内部ELISA与使用粗糙和光滑抗原的玫瑰孟加拉板试验

犬布鲁氏菌和平滑布鲁氏菌感染已在全球犬类中报告。在登革热流行的国家，如基耶病毒国，狗可能同时感染这两种病毒。犬布鲁氏菌病在冰岛的确切发病率尚不清楚。本研究旨在调查基耶省东部凡省绝育流浪狗中布鲁氏菌病的血清患病率。在这项研究中，采用了一种综合方法来确保布鲁氏菌在狗中的可靠发病率。采用光滑型流产布鲁氏菌S99 （RBPT- s）和粗糙型犬布鲁氏菌（RBPT- r）两种抗原的玫瑰孟加拉平板试验（RBPT）对150份犬血清样本进行了分析。对于内部酶联免疫吸附试验（i-ELISA），我们使用了来自粗糙菌株（i-ELISA- r）的粗脂多糖（LPS）抗原和来自英国Weybridge世界动物卫生组织参考实验室的光滑菌株的o -多糖（O-PS）抗原（i-ELISA- s）。每个血清样本都进行了总共四项血清学测试的分析，以确保进行彻底和可靠的评估。在分析的150份血清样本中，32份（21.3% %）使用RBPT-S测试呈阳性，12份（6.6 %）使用RBPT-R测试呈阳性。i-ELISA-R检测到8份（5.3 %）阳性，i-ELISA-S检测到27份（18 %）阳性。在该地区犬中检测到犬b型布鲁氏菌和其他布鲁氏菌感染，表明犬布鲁氏菌病主要由光滑布鲁氏菌引起。基于光滑抗原的测试比基于粗糙抗原的测试识别出更多的阳性样本，这表明两种方法在敏感性或特异性上存在潜在的差异。这种情况被认为对狗和人都是一个重要的风险，强调迫切需要进一步研究和立即实施公共卫生措施来解决这一问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Comparative Immunology Microbiology and Infectious Diseases 医学-免疫学

CiteScore

4.60

自引率

0.00%

发文量

102

审稿时长

40 days

期刊介绍： Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine . The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.