Comparative Immunology Microbiology and Infectious Diseases最新文献

{"title":"Epidemiological profile of human toxocariasis in patients examined at Evandro Chagas Institute (IEC/SVSA/MS) between 2014 and 2019","authors":"Lucas Solano Araújo da Silva ,&nbsp;Isabelle Helena Lima Dias ,&nbsp;Álvaro Luan Santana Fonseca ,&nbsp;Martin Johannes Enk ,&nbsp;Joyce Favacho Cardoso Nogueira ,&nbsp;Ricardo José de Paula Souza e Guimarães ,&nbsp;Christiane de Oliveira Goveia","doi":"10.1016/j.cimid.2023.102112","DOIUrl":"10.1016/j.cimid.2023.102112","url":null,"abstract":"<div><h3>Introduction</h3><p><span>Toxocariasis is caused by nematodes of </span><span><em>Toxocara</em></span><span><span> genus, which infest dogs<span> and cats, with humans serving as </span></span>paratenic hosts.</span></p></div><div><h3>Methods</h3><p>The epidemiological profile of patients examined for toxocariasis between October 2014 and October 2019 at Evandro Chagas Institute (IEC) was outlined. The frequency of anti-<em>T. canis</em><span><span> IgG antibodies were evaluated using the </span>Enzyme Linked Immunosorbent Assay (ELISA) method.</span></p></div><div><h3>Results</h3><p>From a total of 734 samples, 56% were from male (p &lt; 0.05). Regarding age, the group with the most solicitations were from ≤11 years old individuals (p &lt; 0.05). Pará state had the highest number of exams requested (92%), with the majority from residents of urban areas, accounting for 81.5% of samples (p &lt; 0.05). The overall toxocariasis seroprevalence was 41.8%, the male sex being the most frequent with 60.9% (p &lt; 0.05). The most affected age group was ≤11 years old, with a total of 67.8% of positive samples (p &lt; 0.05).</p></div><div><h3>Conclusion</h3><p>The high rates obtained emphasize the need for complementary studies on toxocariasis in Brazil, especially in Pará state, contributing to epidemiological surveillance actions in the control of this infection. Besides, health campaigns for domestic and stray animals, also can contribute to a more effective surveillance in controlling parasitic infections and encourages the One Health approach.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"105 ","pages":"Article 102112"},"PeriodicalIF":2.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139030671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular surveillance of microbial agents from cattle-attached and questing ticks from livestock agroecosystems of Antioquia, Colombia","authors":"Juan A. Segura ,&nbsp;Antonia Dibernardo ,&nbsp;Kathy Manguiat ,&nbsp;Brooks Waitt ,&nbsp;Zulma V. Rueda ,&nbsp;Yoav Keynan ,&nbsp;Heidi Wood ,&nbsp;Lina A. Gutiérrez","doi":"10.1016/j.cimid.2023.102113","DOIUrl":"10.1016/j.cimid.2023.102113","url":null,"abstract":"<div><p><span><span>Ticks are obligate ectoparasites and vectors of pathogens affecting health, </span>agriculture<span>, and animal welfare<span>. This study collected ticks from the cattle and questing ticks of 24 Magdalena Medio Antioquia region cattle farms. Genomic DNA was extracted from the specimens (individual or pools) of the 2088 adult ticks collected from cattle and 4667 immature questing ticks collected from pastures. The molecular detection of </span></span></span><span><em>Babesia</em></span>, <span><em>Anaplasma</em></span>, <span><em>Coxiella</em></span> and <span><em>Rickettsia</em></span><span><span> genera was performed by polymerase chain reaction amplification and subsequent </span>DNA sequencing. In a total of 6755 </span><span><em>Rhipicephalus microplus</em></span> DNA samples, <span><em>Anaplasma marginale</em></span> was the most detected with a frequency of 2% (Confidence Interval- CI 1.68–2.36), followed by <span><em>Babesia bigemina</em></span> with 0.28% (CI 0.16–0.44), <em>Coxiella</em> spp. with 0.15% (CI 0.07–0.27), and <em>Rickettsia</em><span> spp. with 0.13% (CI 0.06–0.25). Molecular analysis of the DNA sequences obtained from the tick samples revealed the presence of </span><em>Coxiella</em><span>-like endosymbiont and </span><span><em>R. </em><em>felis</em></span>. These results demonstrated the diversity of microorganisms present in <em>R. microplus</em><span> ticks predominantly associated with cattle and questing ticks from livestock agroecosystems, suggesting their role as reservoirs and potential biological vectors of these microorganisms on the studied sites. Also, it emphasizes the need to combine acarological surveillance with clinical diagnoses and control strategies on regional and national levels.</span></p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"105 ","pages":"Article 102113"},"PeriodicalIF":2.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138821077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and molecular characterization of two papillomavirus genotypes in teat papillomatosis cases in hair goats, in Türkiye","authors":"Yakup Yıldırım ,&nbsp;Fırat Doğan ,&nbsp;Gökhan Bozkurt ,&nbsp;Ali Küçük ,&nbsp;Gülizar Acar ,&nbsp;Seval Bilge-Dağalp","doi":"10.1016/j.cimid.2023.102111","DOIUrl":"10.1016/j.cimid.2023.102111","url":null,"abstract":"<div><p><span><span><span>Papillomaviruses can cause benign or malignant proliferations on the host’s skin and </span>mucous membranes<span>. Recent genetic studies have identified many new papillomavirus types. In this study, molecular detection and typing was performed on papilloma samples from eight hair goats with </span></span>teat<span> papillomatosis. The papilloma samples were tested with degenerate (FAP59/FAP64,MY09/MY11) and type-specific primers. One sample was positive with degenerate (MY09/MY11) and two samples were positive with C</span></span><em>aprahircus papillomavirus-1</em> type specific primers. The MY09/MY11 primer sequence indicated identity to the closest <em>Ovine papillomavirus type-2</em> (77.9%). The ChPV-1 primer sequence was similar to the closest ChPV-1 (99.4%). Investigating papillomavirus types in different animal species is important for determining the evolution, prevalence, host range, and interspecies transmission potential of papillomaviruses, and to indicate suitable therapeutics for later development.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"104 ","pages":"Article 102111"},"PeriodicalIF":2.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138536751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Equine influenza outbreak in Eastern of Algeria in 2021: The first introduction of Florida Clade 1 to Maghreb area","authors":"Hadda Kareche ,&nbsp;José-Carlos Valle-Casuso ,&nbsp;Anthony Madeline ,&nbsp;Delphine Froger ,&nbsp;Fanny Lecouturier ,&nbsp;Gaëlle Gonzalez ,&nbsp;Abdelaali Debbi ,&nbsp;Saber Taqiyeddine Benseghir ,&nbsp;Abdelkrim Mohamed Nasri ,&nbsp;Moundher Boureghda ,&nbsp;Abdelhamid Achouri ,&nbsp;Farouk Laabassi","doi":"10.1016/j.cimid.2023.102109","DOIUrl":"10.1016/j.cimid.2023.102109","url":null,"abstract":"<div><p><span>We have performed an equine influenza (EI) serological study of the equine population in Algeria by testing 298 serum samples collected between February and August 2021 from 5 provinces. The results were obtained performing an NP-ELISA. Our results revealed that 49.3% (147/298) samples positive for antibodies to EI (H3N8). During this study and after a gap of one decade an outbreak of EI was reported in Algeria in the first week of March 2021. The disease was confirmed by </span>virus<span><span><span> detection from the nasal swabs (n = 39) by qRT-PCR and by identifying 5 EI seroconversion. The virus sequences were identified as </span>H3N8 by sequencing the </span>haemagglutinin<span> (HA) and neuraminidase<span> (NA) genes. Alignment of HA1 amino acid sequence<span> confirmed that viruses belong to Clade 1 of the Florida sublineage in the American lineage. This study indicate the first detection of FC1 strain of EIV in Maghreb area.</span></span></span></span></p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"104 ","pages":"Article 102109"},"PeriodicalIF":2.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138541948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometric, histopathological and molecular findings of Macracanthorhynchus hirudinaceus infection in wild boar (Sus scrofa) from continental Italy","authors":"Giovanni Sgroi ,&nbsp;Nicola D’Alessio ,&nbsp;Antonio Varcasia ,&nbsp;Barbara degli Uberti ,&nbsp;Caterina Fani ,&nbsp;Michele Trotta ,&nbsp;Giovanna Fusco ,&nbsp;Kandai Doi ,&nbsp;Vincenzo Veneziano","doi":"10.1016/j.cimid.2023.102110","DOIUrl":"10.1016/j.cimid.2023.102110","url":null,"abstract":"<div><p>Although <em>Macracanthorhynchus hirudinaceus</em><span><span> is a neglected acanthocephalan of </span>suids<span> occasionally responsible for severe infections in humans, the spread of wild boar (</span></span><span><em>Sus scrofa</em></span><span>) populations in Europe could promote the circulation. Herein, we report the first morphometric, histological and molecular characterization of a severe </span><em>M. hirudinaceus</em><span> infection in a boar from continental Italy. The boar’s intestine displayed granulomatous enteritis<span> due to 24 helminths (14 females, 10 males), identified as adults of </span></span><em>M. hirudinaceus</em><span> by a combined morphometric/molecular approach. The phylogenetic analysis of the </span><em>cox</em>1 gene revealed a close relationship of the <em>M. hirudinaceus</em><span> sequence type found herein with those from Hungary and insular Italy. The high haplotype diversity and low nucleotide diversity of </span><em>M. hirudinaceus</em> specimens would suggest its rapid demographic expansion in the Mediterranean basin. More research is needed to assess the presence of <em>M. hirudinaceus</em> in susceptible beetle species and the role of boars in the epidemiology of infection.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"104 ","pages":"Article 102110"},"PeriodicalIF":2.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138536717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mathematical modeling of the infectious spread and outbreak dynamics of avian influenza with seasonality transmission for chicken farms","authors":"Abdul Malek,&nbsp;Ashabul Hoque","doi":"10.1016/j.cimid.2023.102108","DOIUrl":"10.1016/j.cimid.2023.102108","url":null,"abstract":"<div><p>A compartmental model with a time-varying contact rate, the seasonality effect, and its corresponding nonautonomous model are investigated. The model is developed based on the six compartments: susceptible, latent, infected, asymptomatic, treated, and recovered individuals. We determine the effective reproduction number for this nonautonomous system, and analytic discussion shows that at least one positive periodic solution exists for <span><math><mrow><msub><mrow><mi>R</mi></mrow><mrow><mn>0</mn></mrow></msub><mo>&gt;</mo><mn>1</mn></mrow></math></span>. The model is simulated using the RK-45 numerical method, and the parameter values for the model are taken from the available literature. From the numerical results, we observe that the degree of seasonality and vaccine efficacy significantly impact the amplitude of the epidemic curve<em>.</em> The latent-infected phase plane shows that periodic solutions exhibit a period-doubling bifurcation as the amplitude of seasonality increases. Finally, the model outcome was compared with the actual field data and found to be consistent.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"104 ","pages":"Article 102108"},"PeriodicalIF":2.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138536716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro virulotyping, antifungal susceptibility testing and DNA fingerprinting of Microsporum canis strains of canine and feline origin","authors":"Jitendra Kumar Bagra ,&nbsp;Sonu S. Nair ,&nbsp;V. Athira ,&nbsp;M. Suman Kumar ,&nbsp;Manish Kumar ,&nbsp;Prasad Thomas ,&nbsp;Bablu Kumar ,&nbsp;V.K. Chaturvedi ,&nbsp;Premanshu Dandapat ,&nbsp;Abhishek","doi":"10.1016/j.cimid.2023.102100","DOIUrl":"https://doi.org/10.1016/j.cimid.2023.102100","url":null,"abstract":"<div><p><span><em>Microsporum canis</em></span><span><span> is considered the common dermatophyte agent associated with ringworm in </span>felines<span> and canines. In the present study, we sampled n = 548 felines and canines for the probable isolation of </span></span><em>M. canis</em><span><span>. The rate of isolation from the cats and </span>dogs was 70.27 % (52/74) and 1.68 % (8/474), respectively and Persian cats were found to be highly susceptible to </span><em>M. canis</em><span><span><span> infection. The strains were evaluated for their production of phospholipase<span>, lipase, </span></span>catalase<span><span>, and hemolysis and their ability to grow at 35 ℃. All the strains were identified as low producers of catalase and n = 17 strains exhibited high thermotolerance ability. </span>Terbinafine was found to be the most effective antifungal drug and </span></span>fluconazole was the least effective, </span><em>in vitro</em><span>. AFLP analysis revealed three genotypes of </span><em>M. canis</em><span> with 15 sub-clusters showing ≥ 90 % similarity and 7 sub-clusters exhibiting 100 % similarity. However, the phenotypic characters cannot be attributed based on the AFLP profiles.</span></p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"104 ","pages":"Article 102100"},"PeriodicalIF":2.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138474374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organ distribution and early pathogenesis of Streptococcus equi subsp. zooepidemicus in swine","authors":"Arthur Nery Finatto ,&nbsp;Sulove Koirala ,&nbsp;Fernanda Luiza Facioli ,&nbsp;Jéssica Aparecida Barbosa ,&nbsp;Roman Nosach ,&nbsp;Matheus de Oliveira Costa","doi":"10.1016/j.cimid.2023.102095","DOIUrl":"10.1016/j.cimid.2023.102095","url":null,"abstract":"<div><p><em>Streptococcus equi</em> subsp. <em>zooepidemicus</em> is an emerging pathogen of pigs, resulting in high-mortality outbreaks of septicaemia and abortions. Here, we investigated the early pathogenesis of <em>S. zooepidemicus</em> in pigs following oronasal inoculation. Fourteen pigs were inoculated with live cultures of <em>S. zooepidemicus</em> ST-194, and monitored at 2,4, 8, and 24 h post-inoculation. Necropsies were performed to assess gross lesions and collect samples for bacterial culture and PCR analysis at each time point. Our findings revealed that <em>S. zooepidemicus</em> was detectable in various organs as early as 2 h post-inoculation, including liver and spleen, demonstrating rapid dissemination within the host. Tonsil samples consistently harboured live <em>S. zooepidemicus</em> throughout the study period, suggesting their potential for epidemiological sampling and diagnostics. Moreover, the presence of varying bacterial loads in mesenteric lymph nodes indicated persistence, replication, and a potential source for shedding. Further studies are required to determine the initial site of replication.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"104 ","pages":"Article 102095"},"PeriodicalIF":2.0,"publicationDate":"2023-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147957123001534/pdfft?md5=96ccaea222e0689d347a5c5440a4b260&pid=1-s2.0-S0147957123001534-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138292358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is Brucella excreted in cattle faeces? – Evidence from Punjab, India","authors":"V. Sharma ,&nbsp;P. Kaur ,&nbsp;R.S. Aulakh ,&nbsp;R. Sharma ,&nbsp;R. Verma ,&nbsp;B.B. Singh","doi":"10.1016/j.cimid.2023.102099","DOIUrl":"https://doi.org/10.1016/j.cimid.2023.102099","url":null,"abstract":"<div><p><span>Brucellosis<span> is a neglected zoonosis that affects animals and people in much of the underdeveloped world. The disease is endemic in cattle in Punjab, India and controlling it is a public health challenge. Dairy farmers and farm labour commonly handle cattle faeces with bare hands and personal protective equipments are not used. No studies have been conducted about the shedding of </span></span><span><em>Brucella</em></span> species in faeces of sero positive cattle in the state. This study aimed to isolate and identify the <em>Brucella</em> species from faeces of sero positive cattle in Punjab, India. Faecal samples were collected from 350 <em>Brucella</em> sero positive cattle in Ludhiana district of Punjab, India. Isolation was performed using a pre-enriched <em>Brucella</em> selective broth medium as well as <em>Brucella</em><span> selective medium agar plates containing horse serum and </span><em>Brucella</em><span><span> selective supplements. Isolates were identified using Gram staining technique and rapid slide </span>agglutination test, and then confirmed by using </span><em>bcsp</em><span><span>31 and 16s rRNA genus specific PCR. Isolates were further identified up to species level by using Bruce-Ladder </span>multiplex PCR. Fourteen </span><em>Brucella</em> species were isolated, all of which showed coccobacilli on gram staining, positive rapid slide agglutination test and amplification of <em>bcsp</em><span>31 and 16s rRNA genes. Of the 14 isolates, 11 were identified as </span><em>Brucella abortus</em> and 3 were identified as <span><em>Brucella melitensis</em></span>. The study demonstrates that animal faeces could pose a potential risk for animal and human health and faeces of seropositive cattle must be handled with care.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"104 ","pages":"Article 102099"},"PeriodicalIF":2.0,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138436826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of human created environments in the pathogenic potential and antimicrobial resistance of staphylococci from wild neotropical primates in Brazil","authors":"Indiara Sales ,&nbsp;Olney Vieira-da-Motta ,&nbsp;Ana Tavares ,&nbsp;Carlos Ramón Ruiz-Miranda ,&nbsp;Hermínia de Lencastre ,&nbsp;Maria Miragaia","doi":"10.1016/j.cimid.2023.102094","DOIUrl":"10.1016/j.cimid.2023.102094","url":null,"abstract":"<div><p>The non-human primate (NHP) <em>Leontopithecus rosalia</em> is an endangered species native of Brazil and lives in forest fragments with different levels of contact with humans (natural, private and urban). Other NHPs – <em>Callithrix spp.</em> - were introduced by humans and co-exist and interact with the native species in these forests.</p><p>To evaluate if living in or close to human-modified environments could constitute a risk for <em>L. rosalia</em>, we compared the prevalence, genetic background, antibiotic susceptibility and virulence gene content of staphylococci collected from the native and the introduced species from different forest fragments.</p><p>We found that presence in human-dominated environments increased the colonization rate of <em>L. rosalia</em> with <em>Mammaliicoccus sciuri</em> (former <em>Staphylococcus sciuri</em>) from 18 % to 85 % (p = 0.0001) and of <em>Callithrix spp</em> with <em>Staphylococcus aureus</em> from 6 % to 100 % (p = 0.0001). According to molecular typing data obtained differences probably resulted from dissemination of these bacterial species from the invader NHP species and from humans. Changes in microbiota were paralleled by an increase in the prevalence of Panton-Valentine Leukocidin gene and in resistance to beta-lactams, macrolides and/or lincosamides as exposure to human environment increased. In particular, erythromycin resistance in <em>S. aureus</em> from <em>Callithrix spp</em>. increased from 0 % to 50 % and resistance rate to at least one antibiotic in coagulase-negative staphylococci species from <em>L. rosalia</em> increased from 13 % to 56 % (p = 0.0003).</p><p>Our results showed that contact of native animal species with human-created environments increased the content of antimicrobial resistant and pathogenic bacteria on their commensal microbiota, which ultimately can impact on their health.</p></div><div><h3>Importance</h3><p>Endangered animal species are vulnerable to environmental alterations and human activities have been repeatedly identified as factors driving drastic changes in the natural landscape. It is extremely important to monitor changes in the environment surrounding protected species, because this could lead to early detection of any potential threats. In this study, we found that the contact of <em>L. rosalia -</em> a protected non-human primate from Brazil - with human environments is related to changes in their commensal microbiota. These included an increase in the number of pathogenic and antibiotic resistant bacteria, which have a higher potential to cause infections that are more difficult to treat. We provided evidence for the harmful impact human contact has on <em>L. rosalia</em>. Also, our results suggest that monitoring of commensal microbiota of protected animal species might be a useful way of sensing the risks of protected species to human exposure.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"104 ","pages":"Article 102094"},"PeriodicalIF":2.0,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147957123001522/pdfft?md5=4b452c6958660646dbca1a0dc8452aeb&pid=1-s2.0-S0147957123001522-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138464181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}