Comparative Immunology Microbiology and Infectious Diseases最新文献

{"title":"Molecular investigation of Anaplasma phagocytophilum and related strains among sheep flocks from different parts of Türkiye; with a note of phylogenetic analyses of Anaplasma phagocytophilum- like 1","authors":"Ufuk Erol ,&nbsp;Omer Faruk Sahin ,&nbsp;Osman Furkan Urhan ,&nbsp;Ahmet Duran Atas ,&nbsp;Kursat Altay","doi":"10.1016/j.cimid.2024.102154","DOIUrl":"https://doi.org/10.1016/j.cimid.2024.102154","url":null,"abstract":"<div><p><em>Anaplasma phagocytophilum</em> is a vector-borne zoonotic pathogen and can infect various vertebrate hosts, especially cattle, sheep, goats, horses, and dogs. Molecular-based studies have revealed that the agent has a high genetic diversity and closely related strains circulate in hosts. In this study, 618 sheep blood samples obtained from different geographic regions of Türkiye were researched for <em>A.</em>phagocytophilum and related strains with PCR, RFLP, and DNA sequence analyses. The DNA of these pathogens was detected in 110 (17.79%) samples. RFLP assay showed that all positive samples were infected with A.phagocytophilum-like 1, whereas A.phagocytophilum–like 2 and A.phagocytophilum were not detected. Partial parts of 16 S rRNA gene of seven randomly selected positive samples were sequenced. The phylogenetic analyses of these isolates revealed that at least two A.phagocytophilum-like 1 isolates circulate among hosts in Türkiye and around the world. <em>A.</em>phagocytophilum-related strains have been reported in molecular-based studies over the last few years, but there is a lack of data on the vector competence, epidemiology, clinical symptoms, and genetic diversity of these pathogens. Therefore, large-scale molecular studies are still needed to obtain detailed data on the above-mentioned topics.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102154"},"PeriodicalIF":2.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140030688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The infection of Cysticercus fasciolaris in natural rats (Rattus species) residing in human residence areas, Nakhon Si Thammarat, Thailand","authors":"Sunsaneeya Thaikoed ,&nbsp;Watcharapong Mitsuwan ,&nbsp;Kittipong Chaisiri ,&nbsp;Noppharat Tanthanathipchai ,&nbsp;Maria de Lourdes Pereira ,&nbsp;Alok K. Paul ,&nbsp;Veeranoot Nissapatorn ,&nbsp;Phirabhat Saengsawang","doi":"10.1016/j.cimid.2024.102152","DOIUrl":"https://doi.org/10.1016/j.cimid.2024.102152","url":null,"abstract":"<div><p><em>Cysticercus fasciolaris</em> (<em>C. fasciolaris</em>) is the larval stage of a cestode parasite named <em>Taenia taeniaeformis</em> (<em>T. taeniaeformis</em>). <em>C. fasiolaris</em> is found in small rodents, especially rats. <em>Rattus</em> species are listed as intermediate hosts of this parasite, and cats are the main definitive host of <em>C. fasiolaris.</em> The objective of this study was to study the pathological, microscopic, and molecular aspects of <em>C. fasciolaris</em> in rodents residing in human residence areas. One hundred and two rodents were trapped in human settlements and dissected for larva-containing cyst examinations in the body cavity. The larvae of <em>C. fasciolaris</em> were investigated using histopathological examination, microscopic observations under a stereomicroscope and scanning electron microscope, and molecular detection using polymerase chain reaction. The prevalence of hepatic cysts containing larvae was 8.91% (95% CI = 4.16–16.24). In addition, the older larvae also had longer micropapillae. Histopathological investigation revealed normal hepatic tissue containing larvae and a scanty fluid cyst. The cyst capsule contains mostly mononuclear cells and spindle cells in all infected rats. The molecular detection using two primer sets revealed the amplicons were similar to the clade of <em>C. fasciolaris</em>. In the future, more investigation is necessary to fully understand the parasite's molecular pathogenesis and virulent molecules, which are less obvious.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102152"},"PeriodicalIF":2.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140030687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical importance of neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio and systemic immune-inflammation index in dogs with leishmaniasis","authors":"A. Durán-Galea ,&nbsp;J.I. Cristóbal-Verdejo ,&nbsp;R. Barrera-Chacón ,&nbsp;B. Macías-García ,&nbsp;M.A. González-Solís ,&nbsp;P. Nicolás-Barceló ,&nbsp;A.B. García-Ibáñez ,&nbsp;P. Ruíz-Tapia ,&nbsp;F.J. Duque-Carrasco","doi":"10.1016/j.cimid.2024.102148","DOIUrl":"10.1016/j.cimid.2024.102148","url":null,"abstract":"<div><p>Leishmaniasis is a zoonotic disease caused by Leishmania <em>spp</em>., impacts multiple systems and organs. While hematological and biochemical profiles aren’t definitive for diagnosis, recent studies have identified the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and systemic immune-inflammation index (SII) as predictors of morbidity and mortality in critically ill human and dog patients. This study examined 100 dogs diagnosed with leishmaniasis, categorized by the International Renal Interest Society (IRIS) stages 1–4. Additionally, the dogs were divided based on whether they survived less or more than one year (L1Y and G1Y). Control group consisted of 43 dogs. The NLR increased as the disease progressed (IRIS 1–4), presenting statistically significant differences (P&lt;0.05) when compared to the control group (2,37±2,08) IRIS 3 and 4 (4,59±13,39 and 6,99±12,86, respectively), and G1Y and L1Y (3,60±4,02 and 4,87±5,82, respectively). Significant changes in SII were only evident in short-term survivors (L1Y 951,93±1402) and advanced renal disease cases (IRIS 4 stage 1073,68±1901,09). Conversely, PLR remained largely unchanged. In conclusion, these results suggest that the neutrophil-to-lymphocyte ratio (NLR) and systemic immune-inflammation index (SII) may serve as potential markers for assessing disease progression and prognosis in dogs diagnosed with leishmaniasis.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102148"},"PeriodicalIF":2.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147957124000250/pdfft?md5=84a7166ec3bc64301993faddf590c54b&pid=1-s2.0-S0147957124000250-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139951484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance and public and animal health risks associated with pathogenic Escherichia coli isolated from calves","authors":"Dircéia Aparecida da Costa Custódio ,&nbsp;Carine Rodrigues Pereira ,&nbsp;Maysa Serpa Gonçalves ,&nbsp;Anna Cecília Trolesi Reis Borges Costa ,&nbsp;Pedro Felipe Rodrigues de Oliveira ,&nbsp;Bruna Henrique Pinto da Silva ,&nbsp;Giovanna Botelho Carneiro ,&nbsp;Fernanda Morcatti Coura ,&nbsp;Andrey Pereira Lage ,&nbsp;Marcos Bryan Heinemann ,&nbsp;Geraldo M.árcio da Costa ,&nbsp;Elaine Maria Seles Dorneles","doi":"10.1016/j.cimid.2024.102149","DOIUrl":"10.1016/j.cimid.2024.102149","url":null,"abstract":"<div><p>We aimed to determine the antimicrobial susceptibility profile of pathogenic <em>Escherichia coli</em> strains isolated from fecal samples of calves and buffalo calves (2008–2013), in Minas Gerais, Brazil, as well as the frequency of O157 gene and strains carrying extended-spectrum beta-lactamases (ESBL) and mobile colistin resistance (<em>mcr</em>) genes. <em>E. coli</em> strains (n=518) were tested for susceptibility against ten antimicrobials. Tetracycline was the antimicrobial with the highest resistance rate (382/518), followed by ampicillin (321/518), sulfamethoxazole/trimethoprim (312/518), chloramphenicol (192/518), gentamicin (126/518), ciprofloxacin (148/518), cefazolin (89/518), colistin (54/518) and cefoxitin (34/518). Multidrug resistance (MDR) was observed in 381/518 isolates. No strain harbored <em>mcr</em> or O157 genes<em>,</em> whereas 19/99 were ESBL positive. The most prevalent pathotype and phylogroup were STEC and B1, respectively. Age, EHEC pathotype and resistance to aminoglycoside and cephem were significantly associated with MDR in the multivariate model. Overall, <em>E. coli</em> strains showed high rates of resistance to penicillin, tetracyclines and folate inhibitors, in addition to an alarming rate of MDR and ESBL-producing strains.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102149"},"PeriodicalIF":2.0,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139923211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High diversity, novel genotypes, and vertical transmission of hemotropic Mycoplasma in micromammals","authors":"Javier Millán ,&nbsp;Bárbara Martín-Maldonado ,&nbsp;Ruth Rodríguez-Pastor ,&nbsp;Jesús Martínez-Padilla ,&nbsp;Fernando Esperón","doi":"10.1016/j.cimid.2024.102151","DOIUrl":"10.1016/j.cimid.2024.102151","url":null,"abstract":"<div><p>Hemotropic mycoplasmas (hemoplasmas) are emerging zoonotic pathogens. Micromammals have received little attention as hosts for hemoplasmas despite their ubiquitous presence, high population abundances, and close association with humans. A PCR protocol targeting a fragment of the 16 S rRNA gene and direct sequencing in blood samples of 189 adult specimens and 35 fetuses belonging to three species of Eulipotyphla (shrews) and seven species of Rodentia, captured in three ecologically diverse habitats in North-Eastern Spain (Steppe, High Mountain, Mediterranean) yielded and occurrence of 26%, including 36% of 39 shrews and 23% of 150 rodents. Sequencing revealed the presence of 14 nucleotide sequence types (ntST) among the 56 readable sequences. In general, each ntST was associated with a given host species, although in some cases, the same ntST was sequenced in different species (chiefly rodents). Most ntST were closely related to rodent and/or bat hemoplasmas, but one was identical with <em>Mycoplasma haemocanis</em>/<em>haemofelis</em>, and others can be considered novel genotypes. High sequence diversity was detected in rodents, whereas in the white-toothed shrew (<em>Crocidura russula</em>), 9/11 sequences from two distant areas were identical. Phylogenetic and network analyses classified our sequences in different clades including hemoplasmas of rodents, carnivores, bats, and humans. Twelve of the fetuses (34.2%) of 9/12 litters (75.0%) of shrews and rodents were hemoplasma-positive, indicating frequent vertical transmission. Our study contributes to expanding our knowledge about the distribution, diversity, and transmission of hemoplasmas.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102151"},"PeriodicalIF":2.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147957124000286/pdfft?md5=3954bced5f176c884d4ed047b1a6a3e4&pid=1-s2.0-S0147957124000286-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139923214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of Bartonella rpoB haplotypes in domestic cats from Chile","authors":"Paulina Sepúlveda-García ,&nbsp;Ronald Jara ,&nbsp;Armin Mella ,&nbsp;Gustavo Monti ,&nbsp;Nivia Canales ,&nbsp;Maria Eduarda Chiaradia Furquim ,&nbsp;Marcos Rogério André ,&nbsp;Ananda Müller","doi":"10.1016/j.cimid.2024.102150","DOIUrl":"10.1016/j.cimid.2024.102150","url":null,"abstract":"<div><p>The study aimed to determine the inter and intra-host Bartonella spp. genetic diversity in cats from Chile. ‘Seventy-nine cats’ blood DNA samples qPCR <em>Bartonella</em> spp. positive were subjected to T-A cloning of <em>Bartonella</em> spp. <em>rpoB</em> partial gene (825 bp), and sequencing by Sanger method. The sequences were submitted to phylogenetic and polymorphism analysis. Thirty-six (45.6%) samples were successfully cloned, generating 118 clones of which 109 showed 99.6%–100% identity with <em>Bartonella henselae</em> whereas 9 showed 99.8–100% identity with <em>Bartonella koehlerae</em>. Haplotype analysis yielded 29 different <em>rpoB</em>-<em>B. henselae</em> haplotypes, one (hap#2) overrepresented in 31 out of 33 cats, and 4 <em>rpoB-B. koehlerae</em> haplotypes, with hap#2 represented in all 3 <em>B. koehlerae</em> infected cats. More than one <em>rpoB</em> -<em>B. henselae</em> and <em>B. koehlerae</em> haplotypes were identified in individual cats, reporting by first time coinfection by different <em>B. henselae/B. koehlerae rpoB</em> variants in cats from Chile.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102150"},"PeriodicalIF":2.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139923372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and potential risk factors for Cryptosporidium spp. infection in horses from Egypt","authors":"Mohammed H. Alruhaili ,&nbsp;Mohamed Marzok ,&nbsp;Hattan S. Gattan ,&nbsp;Mohamed Salem ,&nbsp;Mahmoud Kandeel ,&nbsp;Abdelfattah Selim","doi":"10.1016/j.cimid.2024.102140","DOIUrl":"https://doi.org/10.1016/j.cimid.2024.102140","url":null,"abstract":"<div><p><em>Cryptosporidium</em> is an intestinal protozoan that cause diarrhea in livestock all over the world and have zoonotic importance. The present study aimed to determine the prevalence of <em>Cryptosporidium</em> spp. in horses in Egypt and evaluate the associated risk factors. A total of 420 fecal samples were collected from three governorates (Giza, Kafr ElSheikh and Qalyubia) and examined microscopically using Ziehl-Neelsen staining method. The overall prevalence of <em>Cryptosporidium</em> spp. was 29% and Kafr ElSheikh governorate had the highest rate in comparison to other areas. The prevalence of <em>Cryptosporidium</em> spp. in examined horses had significant association with sex, age, type of management, absence of bedding and presence of dogs. The higher prevalence rate was observed in females (32.2%), age group less than two years (43.2%), mixed (grazing and stable) horses (36.1%), animals had history of diarrhea (33%), absence of bedding (35.1%) and contact with dogs (35.7%). These findings give baseline data for further research. It is necessary to establish control strategy for <em>Cryptosporidium</em> spp. infection in order to lower the risk of infection in animals and human.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102140"},"PeriodicalIF":2.0,"publicationDate":"2024-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139726963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of anti-Leptospira spp. antibodies and leptospiruria in cats attended to a veterinary teaching hospital in southern Brazil","authors":"Mirela Grünwalder Paim ,&nbsp;Bárbara Basseggio Rivas ,&nbsp;Grasiele Amorim Sebastião ,&nbsp;Kauana Kaefer ,&nbsp;Rogério Oliveira Rodrigues ,&nbsp;Fabiana Quoos Mayer ,&nbsp;Luciana Neves Nunes ,&nbsp;Fernanda Vieira Amorim da Costa","doi":"10.1016/j.cimid.2024.102138","DOIUrl":"10.1016/j.cimid.2024.102138","url":null,"abstract":"<div><p>Leptospirosis is a bacterial zoonosis that affects both humans and animals worldwide. Currently, it is known that cats may be susceptible to infection. This study aims to investigate the presence of anti-<em>Leptospira</em> spp. antibodies and leptospiruria in cats, using Microscopic Agglutination Test (MAT) and Real-time Polymerase Chain Reaction (PCR) techniques, respectively. A total of 76 cats, undergoing comprehensive anamnesis, general physical examination, and complementary exams were included in the investigation. Among the 76 cats tested, 9.2% (7/76) exhibited the presence of anti-<em>Leptospira</em> spp. antibodies, while <em>Leptospira</em> spp. DNA was detected in at 1.3% (1/76) of the evaluated urine samples. No significant associations were observed between the serological and molecular diagnostic results and the assessed variables, including clinical data and laboratory results of cats testing positive. This study provides insight into the occurrence of <em>Leptospira</em> spp. infection and leptospiruria in cats treated at a veterinary teaching hospital in southern Brazil.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102138"},"PeriodicalIF":2.0,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139666584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beta-lactamase genes in bacteria from food animals, retail meat, and human surveillance programs in the United States from 2002 to 2021","authors":"Md. Kaisar Rahman,&nbsp;Howard Rodriguez-Mori,&nbsp;Guy H. Loneragan,&nbsp;Babafela Awosile","doi":"10.1016/j.cimid.2024.102139","DOIUrl":"10.1016/j.cimid.2024.102139","url":null,"abstract":"<div><p>The spread of beta-lactamase-producing bacteria is a global public-health concern. This study aimed to explore the distribution of beta-lactamases reported in three sampling sources (cecal, retail meat, and human) collected as part of integrated surveillance in the United States. We retrieved and analyzed data from the United States National Antimicrobial Resistance Monitoring Systems (NARMS) from 2002 to 2021. A total of 115 beta-lactamase genes were detected in <em>E. coli</em>, <em>Salmonella enterica</em>, <em>Campylobacter</em>, <em>Shigella</em> and <em>Vibrio</em>: including 35 genes from cecal isolates, 32 genes from the retail meat isolates, and 104 genes from the human isolates. Three genes in <em>E. coli</em> (<em>bla</em>_CMY-2, <em>bla</em>_TEM-1A, and <em>bla</em>_TEM-1B), 6 genes in Salmonella enterica (<em>bla</em>_CARB-2, <em>bla</em>_CMY-2, <em>bla</em>_CTXM-65, <em>bla</em>_TEM-1A, <em>bla</em>_TEM-1B, and <em>bla</em>_HERA-3), and 2 genes in <em>Campylobacter</em> spp. (<em>bla</em>_OXA-61 and <em>bla</em>_OXA-449) have been detected across food animals (cattle, chicken, swine, and turkey) and humans over the study period. <em>bla</em>_CTXM-55 has been detected in <em>E. coli</em> isolates from the four food animal sources while <em>bla</em>_CTXM-15 and <em>bla</em>_CTXM-27 were found only in cattle and swine. In <em>Salmonella enterica</em>, <em>bla</em>_CTXM-2, <em>bla</em>_CTXM-9, <em>bla</em>_CTXM-14, <em>bla</em>_CTXM-15, <em>bla</em>_CTXM-27, <em>bla</em>_CTXM-55, and <em>bla</em>_NDM-1 were only detected among human isolates. <em>bla</em>_OXAs and <em>bla</em>_CARB were bacteria-specific and the only beta-lactamase genes detected in <em>Campylobacter</em> spp. and <em>Vibrio</em> spp respectively. The proportions of beta-lactamase genes detected varies from bacteria to bacteria. This study provided insights on the beta-lactamase genes detected in bacteria in food animals and humans in the United States. This is necessary for better understanding the molecular epidemiology of clinically important beta-lactamases in one health interface.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102139"},"PeriodicalIF":2.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139666937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular prevalence of vector borne bacterial pathogens in the blood samples of wild rodent species trapped from Saudi Arabia","authors":"Abdul Ghafar ,&nbsp;Samia Q. Alghamdi ,&nbsp;Abdullah D. Alanazi ,&nbsp;Syed Muhammad Zukhruf Qousain ,&nbsp;Maryam Ijaz ,&nbsp;Muhammad Naeem ,&nbsp;Muhammad Ali ,&nbsp;Hira Muqaddas ,&nbsp;Adil Khan ,&nbsp;Furhan Iqbal","doi":"10.1016/j.cimid.2024.102129","DOIUrl":"10.1016/j.cimid.2024.102129","url":null,"abstract":"<div><p>Order Rodentia is the most speciose among mammals and the members of this order are known to host more than 60 zoonotic diseases and rodents are a potential health threat to humans. This study was designed to report the molecular prevalence and phylogenetic evaluation of various blood borne bacterial pathogens (<em>Anaplasma ovis, Anaplasma phagocytophilum</em>, <em>Anaplasma marginale</em> and <em>Bartonella</em> spp<em>.</em>) in the blood samples of four wild rodent species [<em>Meriones rex</em> (N = 27), <em>Acomys dimidiatus</em> (N = 18), <em>Myomys yemeni</em> (N = 6) and <em>Rattus rattus</em> (N = 3)] that were trapped during August till October 2020 from Al Makhwah governorate in Saudi Arabia. Results revealed by 9/54 (16.6%) rodents amplified <em>Msp4</em> gene and 2/54 (3.7%) rodents amplified <em>rpoB</em> gene of <em>Anaplasma ovis</em> and <em>Bartonella</em> spp<em>.</em> respectively. <em>Anaplasma phagocytophilum</em> and <em>Anaplasma marginale</em> were not detected among enrolled rodent species. <em>Meriones rex</em> was the most highly infected rodent species. DNA sequencing and BLAST analysis confirmed the presence of <em>Anaplasma ovis</em> and the <em>Bartonella koehlerae</em> in rodent blood samples. Phylogenetic analysis of both pathogens showed that Saudi isolates were clustered together and were closely related to isolates that were reported from worldwide countries. Risk factor analysis revealed that prevalence of both bacterial pathogens was not restricted to a particular rodent species or a rodent sex (P &gt; 0.05). In conclusion, we are reporting for the very first time that Saudi rodents are infected with <em>Anaplasma ovis</em> and rodents can be infected with <em>Bartonella koehlerae</em>. Similar studies at large scale are recommended in all those areas of Saudi Arabia that are unexplored for the incidence and prevalence of bacterial pathogens among the rodents that are living near human dwellings in order to prevent bacterial infections in local people as well as in livestock<em>.</em></p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"106 ","pages":"Article 102129"},"PeriodicalIF":2.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139580279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}