Comparative Immunology Microbiology and Infectious Diseases最新文献

{"title":"Prevalence and molecular characterization of Trichomonas gallinae from pigeons in Anhui, China","authors":"Yilei Zhang ,&nbsp;Zhenxiao Lu ,&nbsp;Zhenzhen Liu ,&nbsp;Yuhan Zhou ,&nbsp;Guodong Xiao ,&nbsp;Ayanniyi Olalekan Opeyemi ,&nbsp;Sihua Jin ,&nbsp;Yongsheng Li ,&nbsp;Tao Liu ,&nbsp;Qiong Wu ,&nbsp;Xingdong Sun ,&nbsp;Qianming Xu ,&nbsp;Qingxun Zhang ,&nbsp;Congshan Yang","doi":"10.1016/j.cimid.2024.102157","DOIUrl":"https://doi.org/10.1016/j.cimid.2024.102157","url":null,"abstract":"<div><p><em>Trichomonas gallinae</em>, a protozoan parasite causing avian trichomonosis, exhibits a widespread global prevalence. It primarily affects the upper digestive tract of birds and has resulted in significant ecological problems worldwide. This study aimed to investigate the prevalence and genotypes of <em>T. gallinae</em> in Anhui Province, China. A total of 1612 oropharyngeal swab samples were collected from pigeon farms in Anhui Province to determine the prevalence of <em>T. gallinae</em> infection. The results revealed 565 (35.1%) positive samples of <em>T. gallinae</em>. Significant differences in infection rates were observed among different regions and age groups. Furthermore, the ITS1/5.8 S/ITS2 region was amplified, sequenced, and subjected to phylogenetic analysis. Genotypes A and B of <em>T. gallinae</em> were identified, and genotype B was the dominant genotype in Anhui Province. This is the first report on the prevalence and molecular characterization of <em>T. gallinae</em> in Anhui Province, China. Additionally, we integrated reports on the prevalence and genotype of <em>T. gallinae</em> in relevant provinces in China.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102157"},"PeriodicalIF":2.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140122573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of pathogenic Leptospira spp. in unconventional pets","authors":"Bruna Carolina Ulsenheimer ,&nbsp;Matheus Yuri dos Santos ,&nbsp;Bruna Della Flora ,&nbsp;Diego Andres Rodriguez Matarrita ,&nbsp;Sônia de Avila Botton ,&nbsp;Ana Eucares Von Laer ,&nbsp;Daniela Isabel Brayer Pereira ,&nbsp;Rafael Almeida Fighera ,&nbsp;Alexandre Alberto Tonin","doi":"10.1016/j.cimid.2024.102158","DOIUrl":"10.1016/j.cimid.2024.102158","url":null,"abstract":"<div><p>Leptospirosis is a disease caused by <em>Leptospira</em> spp. responsible for considerable impacts on the public and animal health. In the past two decades, non-domesticated species of pets (unconventional pets) have become popular. However, the role of these unconventional pets on maintaining diseases still unclear. Therefore, the objective of this study was to survey the presence of <em>Leptospira</em> spp. DNA in unconventional pets. Samples of kidney tissues from 29 animals belonging to the Mammalia class (including Orders Carnivora, Lagomorpha and Rodentia) were analyzed for the presence of the gene <em>lipL32</em>. As a result, DNA of pathogenic <em>Leptospira</em> spp. from specie <em>L. interrogans</em> was detected in four (13,80%) of the analyzed samples: three from <em>Oryctolagus cuniculus</em> and one from <em>Mesocricetus auratus</em>. This study highlights the importance of epidemiological surveillance of leptospirosis, as it identified in species of unconventional pets, that may possibly act as reservoirs of <em>Leptospira</em> spp.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"108 ","pages":"Article 102158"},"PeriodicalIF":2.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140125092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New records of pathogenic bacteria in different species of fleas collected from domestic and peridomestic animals in Spain. A potential zoonotic threat?","authors":"Antonio Zurita,&nbsp;Ignacio Trujillo,&nbsp;Cristina Cutillas","doi":"10.1016/j.cimid.2024.102153","DOIUrl":"10.1016/j.cimid.2024.102153","url":null,"abstract":"<div><p>Climate change is causing many vectors of infectious diseases to expand their geographic distribution as well as the pathogens they transmit are also conditioned by temperature for their multiplication. Within this context, it is worth highlighting the significant role that fleas can play as vectors of important pathogenic bacteria. For this purpose, our efforts focused on detecting and identifying a total of 9 bacterial genera (<em>Rickettsia</em> sp.; <em>Bartonella</em> sp.; <em>Yersinia</em> sp.; <em>Wolbachia</em> sp., <em>Mycobacterium</em> sp., <em>Leishmania</em> sp., <em>Borrelia</em> sp., <em>Francisella</em> sp. and <em>Coxiella</em> sp.) within fleas isolated from domestic and peridomestic animals in the southwestern region of Spain (Andalusia). Over a 19-months period, we obtained flea samples from dogs, cats and hedgehogs. A total of 812 fleas was collected for this study. Five different species were morphologically identified, including <em>C. felis</em>, <em>C. canis</em>, <em>S. cuniculi</em>, <em>P. irritans</em>, and <em>A. erinacei</em>. <em>Wolbachia</em> sp. was detected in all five species identified in our study which a total prevalence of 86%. Within <em>Rickettsia</em> genus, two different species, <em>R. felis</em> and <em>R. asembonensis</em> were mainly identified in <em>C. felis</em> and <em>A. erinacei</em>, respectively. On the other hand, our results revealed a total of 131 fleas testing positive for the presence of <em>Bartonella</em> sp., representing a prevalence rate of 16% for this genus identifying two species <em>B. henselae</em> and <em>B. clarridgeiae</em>. Lastly, both <em>Y. pestis</em> and <em>L. infantum</em> were detected in DNA of <em>P. irritans</em> and <em>C. felis</em>, respectively isolated from dogs. With these data we update the list of bacterial zoonotic agents found in fleas in Spain, emphasizing the need to continue conducting future experimental studies to assess and confirm the potential vectorial role of certain synanthropic fleas.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102153"},"PeriodicalIF":2.0,"publicationDate":"2024-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147957124000304/pdfft?md5=d5d978026f6adba2fe3c4d51ac3b59e3&pid=1-s2.0-S0147957124000304-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140057387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence and associated risk factors of equine herpesvirus type-1/-4 in selected districts of Northwest Amhara, Ethiopia","authors":"Anmut Worku ,&nbsp;Wassie Molla ,&nbsp;Ambaye Kenubih ,&nbsp;Daniel Gizaw ,&nbsp;Ayelech Muluneh ,&nbsp;Bemrew Admassu ,&nbsp;Mebrat Ejo ,&nbsp;Gashaw Getaneh Dagnaw ,&nbsp;Abebe Belete Bitew ,&nbsp;Tewodros Fentahun ,&nbsp;Kalkidan Getnet ,&nbsp;Haileyesus Dejene ,&nbsp;Kassahun Berrie ,&nbsp;Saddam Mohammed Ibrahim ,&nbsp;Abebe Tesfaye Gessese ,&nbsp;Melkie Dagnaw Fenta ,&nbsp;Bereket Dessalegn ,&nbsp;Mastewal Birhan ,&nbsp;Mebrie Zemene Kinde","doi":"10.1016/j.cimid.2024.102155","DOIUrl":"10.1016/j.cimid.2024.102155","url":null,"abstract":"<div><p>This study aimed to estimate the prevalence, determine the distribution, and identify the epidemiological risk factors of EHV-1/-4 infections in selected districts of Northwest Amhara Region. 460 serum samples were collected from equines using multistage cluster sampling technique, and a competitive Enzyme-linked immunosorbent assay (cELISA) was performed. Various risk factors for the occurrence of EHV-1/-4 were considered. Statistical analysis was performed using R version 4.3.1. 65.9% (303) equids were tested positive for antibodies against EHV-1/-4. Based on district, the highest prevalence was recorded in Wogera (86.1%), while the lowest was in Debark (47.4%). There was a significant difference (p &lt;0.05; 95% CI: 1.1067993–3.682843) in the prevalence of EHV-1/-4 among species and donkeys are 2.019 times more likely to get an EHV infection than horses. The prevalence of EHV-1/-4 was highest in equids with the age of 3–8 years and lowest in &lt; 3 years, and the difference was statistically significant (p &lt;0.05; 95% CI: 1.9812042–6.771820). Statistically significant variation (p &lt;0.05; 95% CI: 1.1173822–2.684013) was also observed between sex of equids in which females had 1.73 times higher chance to get EHV infection than males. Higher prevalence was found in lactating equids (81.6%), followed by pregnant equids (74.6%), and dry equids (66.4%). Generally, this study indicated a high and wide distribution of EHV-1/-4 infection in the study area, which needs due attention. Devising strategies to prevent and minimize the spread and occurrence of the infection is crucial.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102155"},"PeriodicalIF":2.0,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140057396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal dynamics and genetic characterization of bovine arthropod-borne parasites in Nan Province, Thailand with molecular identification of Anaplasma platys and Trypanosoma theileri","authors":"Apinya Arnuphapprasert ,&nbsp;Yudhi Ratna Nugraheni ,&nbsp;Sarawanee Khunmanee ,&nbsp;Winai Kaewlamun ,&nbsp;Morakot Kaewthamasorn","doi":"10.1016/j.cimid.2024.102156","DOIUrl":"10.1016/j.cimid.2024.102156","url":null,"abstract":"<div><p>Virulent species or strains of hematophagous borne pathogens such as <em>Anaplasma</em> spp., <em>Babesia</em> spp., <em>Theileria</em> spp., and <em>Trypanosoma</em> spp., are lethal to susceptible animals or reduce their productivity on a global scale. Nonetheless, efforts to diagnose the causative agents and assess the genotypic profiles as well as quantify the parasite burden of aforementioned parasites across seasons remain limited. Therefore, the present investigation sought to elucidate the genotypic composition of <em>Anaplasma</em> spp., <em>Babesia</em> spp., <em>Theileria</em> spp., and <em>Trypanosoma</em> spp. The findings revealed heightened infection rates during the summer, manifesting a correlation between <em>Trypanosoma</em> spp. infection and seasonal fluctuations. Among the identified pathogens, <em>Anaplasma marginale</em> emerged as the most dominant species, while the occurrence of <em>Anaplasma platys</em> in Thai cattle was confirmed via the sequencing of the <em>groEL</em> gene. Moreover, the study successfully identified two lineages of <em>Trypanosoma theileri</em>. The findings of this investigation offer valuable insights that can inform the development of preventive strategies for vector-borne diseases, such as considering the appropriate use of insect repellent, mosquito or insect nets, or eliminating breeding places for insects in each season.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102156"},"PeriodicalIF":2.0,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140057411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular investigation of Anaplasma phagocytophilum and related strains among sheep flocks from different parts of Türkiye; with a note of phylogenetic analyses of Anaplasma phagocytophilum- like 1","authors":"Ufuk Erol ,&nbsp;Omer Faruk Sahin ,&nbsp;Osman Furkan Urhan ,&nbsp;Ahmet Duran Atas ,&nbsp;Kursat Altay","doi":"10.1016/j.cimid.2024.102154","DOIUrl":"https://doi.org/10.1016/j.cimid.2024.102154","url":null,"abstract":"<div><p><em>Anaplasma phagocytophilum</em> is a vector-borne zoonotic pathogen and can infect various vertebrate hosts, especially cattle, sheep, goats, horses, and dogs. Molecular-based studies have revealed that the agent has a high genetic diversity and closely related strains circulate in hosts. In this study, 618 sheep blood samples obtained from different geographic regions of Türkiye were researched for <em>A.</em>phagocytophilum and related strains with PCR, RFLP, and DNA sequence analyses. The DNA of these pathogens was detected in 110 (17.79%) samples. RFLP assay showed that all positive samples were infected with A.phagocytophilum-like 1, whereas A.phagocytophilum–like 2 and A.phagocytophilum were not detected. Partial parts of 16 S rRNA gene of seven randomly selected positive samples were sequenced. The phylogenetic analyses of these isolates revealed that at least two A.phagocytophilum-like 1 isolates circulate among hosts in Türkiye and around the world. <em>A.</em>phagocytophilum-related strains have been reported in molecular-based studies over the last few years, but there is a lack of data on the vector competence, epidemiology, clinical symptoms, and genetic diversity of these pathogens. Therefore, large-scale molecular studies are still needed to obtain detailed data on the above-mentioned topics.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102154"},"PeriodicalIF":2.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140030688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The infection of Cysticercus fasciolaris in natural rats (Rattus species) residing in human residence areas, Nakhon Si Thammarat, Thailand","authors":"Sunsaneeya Thaikoed ,&nbsp;Watcharapong Mitsuwan ,&nbsp;Kittipong Chaisiri ,&nbsp;Noppharat Tanthanathipchai ,&nbsp;Maria de Lourdes Pereira ,&nbsp;Alok K. Paul ,&nbsp;Veeranoot Nissapatorn ,&nbsp;Phirabhat Saengsawang","doi":"10.1016/j.cimid.2024.102152","DOIUrl":"https://doi.org/10.1016/j.cimid.2024.102152","url":null,"abstract":"<div><p><em>Cysticercus fasciolaris</em> (<em>C. fasciolaris</em>) is the larval stage of a cestode parasite named <em>Taenia taeniaeformis</em> (<em>T. taeniaeformis</em>). <em>C. fasiolaris</em> is found in small rodents, especially rats. <em>Rattus</em> species are listed as intermediate hosts of this parasite, and cats are the main definitive host of <em>C. fasiolaris.</em> The objective of this study was to study the pathological, microscopic, and molecular aspects of <em>C. fasciolaris</em> in rodents residing in human residence areas. One hundred and two rodents were trapped in human settlements and dissected for larva-containing cyst examinations in the body cavity. The larvae of <em>C. fasciolaris</em> were investigated using histopathological examination, microscopic observations under a stereomicroscope and scanning electron microscope, and molecular detection using polymerase chain reaction. The prevalence of hepatic cysts containing larvae was 8.91% (95% CI = 4.16–16.24). In addition, the older larvae also had longer micropapillae. Histopathological investigation revealed normal hepatic tissue containing larvae and a scanty fluid cyst. The cyst capsule contains mostly mononuclear cells and spindle cells in all infected rats. The molecular detection using two primer sets revealed the amplicons were similar to the clade of <em>C. fasciolaris</em>. In the future, more investigation is necessary to fully understand the parasite's molecular pathogenesis and virulent molecules, which are less obvious.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102152"},"PeriodicalIF":2.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140030687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical importance of neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio and systemic immune-inflammation index in dogs with leishmaniasis","authors":"A. Durán-Galea ,&nbsp;J.I. Cristóbal-Verdejo ,&nbsp;R. Barrera-Chacón ,&nbsp;B. Macías-García ,&nbsp;M.A. González-Solís ,&nbsp;P. Nicolás-Barceló ,&nbsp;A.B. García-Ibáñez ,&nbsp;P. Ruíz-Tapia ,&nbsp;F.J. Duque-Carrasco","doi":"10.1016/j.cimid.2024.102148","DOIUrl":"10.1016/j.cimid.2024.102148","url":null,"abstract":"<div><p>Leishmaniasis is a zoonotic disease caused by Leishmania <em>spp</em>., impacts multiple systems and organs. While hematological and biochemical profiles aren’t definitive for diagnosis, recent studies have identified the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR) and systemic immune-inflammation index (SII) as predictors of morbidity and mortality in critically ill human and dog patients. This study examined 100 dogs diagnosed with leishmaniasis, categorized by the International Renal Interest Society (IRIS) stages 1–4. Additionally, the dogs were divided based on whether they survived less or more than one year (L1Y and G1Y). Control group consisted of 43 dogs. The NLR increased as the disease progressed (IRIS 1–4), presenting statistically significant differences (P&lt;0.05) when compared to the control group (2,37±2,08) IRIS 3 and 4 (4,59±13,39 and 6,99±12,86, respectively), and G1Y and L1Y (3,60±4,02 and 4,87±5,82, respectively). Significant changes in SII were only evident in short-term survivors (L1Y 951,93±1402) and advanced renal disease cases (IRIS 4 stage 1073,68±1901,09). Conversely, PLR remained largely unchanged. In conclusion, these results suggest that the neutrophil-to-lymphocyte ratio (NLR) and systemic immune-inflammation index (SII) may serve as potential markers for assessing disease progression and prognosis in dogs diagnosed with leishmaniasis.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102148"},"PeriodicalIF":2.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147957124000250/pdfft?md5=84a7166ec3bc64301993faddf590c54b&pid=1-s2.0-S0147957124000250-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139951484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance and public and animal health risks associated with pathogenic Escherichia coli isolated from calves","authors":"Dircéia Aparecida da Costa Custódio ,&nbsp;Carine Rodrigues Pereira ,&nbsp;Maysa Serpa Gonçalves ,&nbsp;Anna Cecília Trolesi Reis Borges Costa ,&nbsp;Pedro Felipe Rodrigues de Oliveira ,&nbsp;Bruna Henrique Pinto da Silva ,&nbsp;Giovanna Botelho Carneiro ,&nbsp;Fernanda Morcatti Coura ,&nbsp;Andrey Pereira Lage ,&nbsp;Marcos Bryan Heinemann ,&nbsp;Geraldo M.árcio da Costa ,&nbsp;Elaine Maria Seles Dorneles","doi":"10.1016/j.cimid.2024.102149","DOIUrl":"10.1016/j.cimid.2024.102149","url":null,"abstract":"<div><p>We aimed to determine the antimicrobial susceptibility profile of pathogenic <em>Escherichia coli</em> strains isolated from fecal samples of calves and buffalo calves (2008–2013), in Minas Gerais, Brazil, as well as the frequency of O157 gene and strains carrying extended-spectrum beta-lactamases (ESBL) and mobile colistin resistance (<em>mcr</em>) genes. <em>E. coli</em> strains (n=518) were tested for susceptibility against ten antimicrobials. Tetracycline was the antimicrobial with the highest resistance rate (382/518), followed by ampicillin (321/518), sulfamethoxazole/trimethoprim (312/518), chloramphenicol (192/518), gentamicin (126/518), ciprofloxacin (148/518), cefazolin (89/518), colistin (54/518) and cefoxitin (34/518). Multidrug resistance (MDR) was observed in 381/518 isolates. No strain harbored <em>mcr</em> or O157 genes<em>,</em> whereas 19/99 were ESBL positive. The most prevalent pathotype and phylogroup were STEC and B1, respectively. Age, EHEC pathotype and resistance to aminoglycoside and cephem were significantly associated with MDR in the multivariate model. Overall, <em>E. coli</em> strains showed high rates of resistance to penicillin, tetracyclines and folate inhibitors, in addition to an alarming rate of MDR and ESBL-producing strains.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102149"},"PeriodicalIF":2.0,"publicationDate":"2024-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139923211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High diversity, novel genotypes, and vertical transmission of hemotropic Mycoplasma in micromammals","authors":"Javier Millán ,&nbsp;Bárbara Martín-Maldonado ,&nbsp;Ruth Rodríguez-Pastor ,&nbsp;Jesús Martínez-Padilla ,&nbsp;Fernando Esperón","doi":"10.1016/j.cimid.2024.102151","DOIUrl":"10.1016/j.cimid.2024.102151","url":null,"abstract":"<div><p>Hemotropic mycoplasmas (hemoplasmas) are emerging zoonotic pathogens. Micromammals have received little attention as hosts for hemoplasmas despite their ubiquitous presence, high population abundances, and close association with humans. A PCR protocol targeting a fragment of the 16 S rRNA gene and direct sequencing in blood samples of 189 adult specimens and 35 fetuses belonging to three species of Eulipotyphla (shrews) and seven species of Rodentia, captured in three ecologically diverse habitats in North-Eastern Spain (Steppe, High Mountain, Mediterranean) yielded and occurrence of 26%, including 36% of 39 shrews and 23% of 150 rodents. Sequencing revealed the presence of 14 nucleotide sequence types (ntST) among the 56 readable sequences. In general, each ntST was associated with a given host species, although in some cases, the same ntST was sequenced in different species (chiefly rodents). Most ntST were closely related to rodent and/or bat hemoplasmas, but one was identical with <em>Mycoplasma haemocanis</em>/<em>haemofelis</em>, and others can be considered novel genotypes. High sequence diversity was detected in rodents, whereas in the white-toothed shrew (<em>Crocidura russula</em>), 9/11 sequences from two distant areas were identical. Phylogenetic and network analyses classified our sequences in different clades including hemoplasmas of rodents, carnivores, bats, and humans. Twelve of the fetuses (34.2%) of 9/12 litters (75.0%) of shrews and rodents were hemoplasma-positive, indicating frequent vertical transmission. Our study contributes to expanding our knowledge about the distribution, diversity, and transmission of hemoplasmas.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"107 ","pages":"Article 102151"},"PeriodicalIF":2.0,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0147957124000286/pdfft?md5=3954bced5f176c884d4ed047b1a6a3e4&pid=1-s2.0-S0147957124000286-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139923214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}