A cross sectional study on molecular prevalence of Orientia tsutsugamushi in household rat population of South India

IF 2 3区农林科学 Q4 IMMUNOLOGY

Comparative Immunology Microbiology and Infectious Diseases Pub Date : 2024-06-14 DOI:10.1016/j.cimid.2024.102212

S. Purushothaman , P. Azhahianambi , M. Dharman , R. Gokula Kannan , K.G. Tirumurugaan , C. Soundararajan , R.P. Aravindh Babu , Panneer Devaraju , John A.J. Prakash

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Abstract

This study aimed to assess the molecular prevalence of mite-borne zoonotic pathogen O. tsutsugamushi in household rats of South India through nested polymerase chain reaction amplification of O. tsutsugamushi 47-kDa htrA gene and to determine the most suitable sample type for screening of O. tsutsugamushi in rats. Out of 85 rats trapped in Tamil Nadu, Karnataka, and Puducherry regions, 47 rats were found positive for the O. tsutsugamushi genome with prevalence of 55.29 %. Among different sample types screened, faecal samples exhibited the highest positivity rate, followed by liver, spleen, kidney, and blood samples. Agreement between faecal and spleen samples of rats for the presence of O. tsutsugamushi was the highest. Principal component analysis revealed a positive correlation between the spleen, liver, and faeces and a negative correlation between blood and faeces for the presence of O. tsutsugamushi genome. These findings underscore the varied distribution of O. tsutsugamushi among different samples and indicate that the faecal and liver samples of rats are an ideal choice of samples for epidemiological studies. This is the first study to report a high level of presence of O. tsutsugamushi in faecal samples of rats.

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关于南印度家庭鼠群中恙虫病分子流行率的横断面研究。

本研究旨在通过巢式聚合酶链反应扩增恙虫病 47-kDa htrA 基因，评估恙虫病在南印度家鼠中的分子流行率，并确定最适合筛查家鼠中恙虫病的样本类型。在泰米尔纳德邦、卡纳塔克邦和普度克里地区捕获的 85 只大鼠中，发现 47 只大鼠的恙虫病基因组呈阳性，感染率为 55.29%。在筛查的不同样本类型中，粪便样本的阳性率最高，其次是肝脏、脾脏、肾脏和血液样本。大鼠粪便样本和脾脏样本与恙虫病菌存在的一致性最高。主成分分析表明，脾脏、肝脏和粪便之间存在正相关，而血液和粪便之间存在负相关。这些发现强调了恙虫卵在不同样本中的不同分布，并表明大鼠的粪便和肝脏样本是流行病学研究的理想样本选择。这是首次报告大鼠粪便样本中存在大量恙虫病的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Comparative Immunology Microbiology and Infectious Diseases 医学-免疫学

CiteScore

4.60

自引率

0.00%

发文量

102

审稿时长

40 days

期刊介绍： Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine . The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.