Advances in Virus Research最新文献_第4页

Shedding light on reovirus assembly-Multimodal imaging of viral factories. 揭示呼肠孤病毒组装——病毒工厂的多模态成像。

2区医学

Advances in Virus Research Pub Date : 2023-01-01 DOI: 10.1016/bs.aivir.2023.06.002

Eva Durinova, Peter Mojzes, Tomas Bily, Zdenek Franta, Tomas Fessl, Alexander Borodavka, Roman Tuma

{"title":"Shedding light on reovirus assembly-Multimodal imaging of viral factories.","authors":"Eva Durinova, Peter Mojzes, Tomas Bily, Zdenek Franta, Tomas Fessl, Alexander Borodavka, Roman Tuma","doi":"10.1016/bs.aivir.2023.06.002","DOIUrl":"https://doi.org/10.1016/bs.aivir.2023.06.002","url":null,"abstract":"Avian (ortho)reovirus (ARV), which belongs to Reoviridae family, is a major domestic fowl pathogen and is the causative agent of viral tenosynovitis and chronic respiratory disease in chicken. ARV replicates within cytoplasmic inclusions, so-called viral factories, that form by phase separation and thus belong to a wider class of biological condensates. Here, we evaluate different optical imaging methods that have been developed or adapted to follow formation, fluidity and composition of viral factories and compare them with the complementary structural information obtained by well-established transmission electron microscopy and electron tomography. The molecular and cellular biology aspects for setting up and following virus infection in cells by imaging are described first. We then demonstrate that a wide-field version of fluorescence recovery after photobleaching is an effective tool to measure fluidity of mobile viral factories. A new technique, holotomographic phase microscopy, is then used for imaging of viral factory formation in live cells in three dimensions. Confocal Raman microscopy of infected cells provides \"chemical\" contrast for label-free segmentation of images and addresses important questions about biomolecular concentrations within viral factories and other biological condensates. Optical imaging is complemented by electron microscopy and tomography which supply higher resolution structural detail, including visualization of individual virions within the three-dimensional cellular context.","PeriodicalId":50977,"journal":{"name":"Advances in Virus Research","volume":"116 ","pages":"173-213"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10277151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From the beginnings to multidimensional light and electron microscopy of virus morphogenesis. 从开始到多维光和电子显微镜下的病毒形态发生。

2区医学

Advances in Virus Research Pub Date : 2023-01-01 DOI: 10.1016/bs.aivir.2023.05.001

Saskia Sanders, Yannick Jensen, Rudolph Reimer, Jens B Bosse

引用次数: 0

Microtubules and viral infection. 微管和病毒感染

2区医学

Advances in Virus Research Pub Date : 2023-01-01 Epub Date: 2023-04-03 DOI: 10.1016/bs.aivir.2023.02.003

Eveline Santos da Silva, Mojgan H Naghavi

引用次数: 0

Advanced fluorescence microscopy in respiratory virus cell biology. 呼吸道病毒细胞生物学中的高级荧光显微镜。

2区医学

Advances in Virus Research Pub Date : 2023-01-01 DOI: 10.1016/bs.aivir.2023.05.002

Enyu Xie, Shazeb Ahmad, Redmond P Smyth, Christian Sieben

{"title":"Advanced fluorescence microscopy in respiratory virus cell biology.","authors":"Enyu Xie, Shazeb Ahmad, Redmond P Smyth, Christian Sieben","doi":"10.1016/bs.aivir.2023.05.002","DOIUrl":"https://doi.org/10.1016/bs.aivir.2023.05.002","url":null,"abstract":"Respiratory viruses are a major public health burden across all age groups around the globe, and are associated with high morbidity and mortality rates. They can be transmitted by multiple routes, including physical contact or droplets and aerosols, resulting in efficient spreading within the human population. Investigations of the cell biology of virus replication are thus of utmost importance to gain a better understanding of virus-induced pathogenicity and the development of antiviral countermeasures. Light and fluorescence microscopy techniques have revolutionized investigations of the cell biology of virus infection by allowing the study of the localization and dynamics of viral or cellular components directly in infected cells. Advanced microscopy including high- and super-resolution microscopy techniques available today can visualize biological processes at the single-virus and even single-molecule level, thus opening a unique view on virus infection. We will highlight how fluorescence microscopy has supported investigations on virus cell biology by focusing on three major respiratory viruses: respiratory syncytial virus (RSV), Influenza A virus (IAV) and SARS-CoV-2. We will review our current knowledge of virus replication and highlight how fluorescence microscopy has helped to improve our state of understanding. We will start by introducing major imaging and labeling modalities and conclude the chapter with a perspective discussion on remaining challenges and potential opportunities.","PeriodicalId":50977,"journal":{"name":"Advances in Virus Research","volume":"116 ","pages":"123-172"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10295161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial resolution of virus replication: RSV and cytoplasmic inclusion bodies. 病毒复制的空间分辨率:RSV和细胞质包涵体。

2区医学

Advances in Virus Research Pub Date : 2023-01-01 DOI: 10.1016/bs.aivir.2023.06.001

Jennifer Risso-Ballester, Marie-Anne Rameix-Welti

{"title":"Spatial resolution of virus replication: RSV and cytoplasmic inclusion bodies.","authors":"Jennifer Risso-Ballester, Marie-Anne Rameix-Welti","doi":"10.1016/bs.aivir.2023.06.001","DOIUrl":"https://doi.org/10.1016/bs.aivir.2023.06.001","url":null,"abstract":"Respiratory Syncytial Virus (RSV) is a major cause of respiratory illness in young children, elderly and immunocompromised individuals worldwide representing a severe burden for health systems. The urgent development of vaccines or specific antivirals against RSV is impaired by the lack of knowledge regarding its replication mechanisms. RSV is a negative-sense single-stranded RNA (ssRNA) virus belonging to the Mononegavirales order (MNV) which includes other viruses pathogenic to humans as Rabies (RabV), Ebola (EBOV), or measles (MeV) viruses. Transcription and replication of viral genomes occur within cytoplasmatic virus-induced spherical inclusions, commonly referred as inclusion bodies (IBs). Recently IBs were shown to exhibit properties of membrane-less organelles (MLO) arising by liquid-liquid phase separation (LLPS). Compartmentalization of viral RNA synthesis steps in viral-induced MLO is indeed a common feature of MNV. Strikingly these key compartments still remain mysterious. Most of our current knowledge on IBs relies on the use of fluorescence microscopy. The ability to fluorescently label IBs in cells has been key to uncover their dynamics and nature. The generation of recombinant viruses expressing a fluorescently-labeled viral protein and the immunolabeling or the expression of viral fusion proteins known to be recruited in IBs are some of the tools used to visualize IBs in infected cells. In this chapter, microscope techniques and the most relevant studies that have shed light on RSV IBs fundamental aspects, including biogenesis, organization and dynamics are being discussed and brought to light with the investigations carried out on other MNV.","PeriodicalId":50977,"journal":{"name":"Advances in Virus Research","volume":"116 ","pages":"1-43"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10603229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycoviruses as a part of the global virome: Diversity, evolutionary links and lifestyle. 分枝病毒作为全球病毒组的一部分:多样性、进化联系和生活方式。

2区医学

Advances in Virus Research Pub Date : 2023-01-01 DOI: 10.1016/bs.aivir.2023.02.002

María A Ayllón, Eeva J Vainio

{"title":"Mycoviruses as a part of the global virome: Diversity, evolutionary links and lifestyle.","authors":"María A Ayllón, Eeva J Vainio","doi":"10.1016/bs.aivir.2023.02.002","DOIUrl":"https://doi.org/10.1016/bs.aivir.2023.02.002","url":null,"abstract":"Knowledge of mycovirus diversity, evolution, horizontal gene transfer and shared ancestry with viruses infecting distantly related hosts, such as plants and arthropods, has increased vastly during the last few years due to advances in the high throughput sequencing methodologies. This also has enabled the discovery of novel mycoviruses with previously unknown genome types, mainly new positive and negative single-stranded RNA mycoviruses ((+) ssRNA and (-) ssRNA) and single-stranded DNA mycoviruses (ssDNA), and has increased our knowledge of double-stranded RNA mycoviruses (dsRNA), which in the past were thought to be the most common viruses infecting fungi. Fungi and oomycetes (Stramenopila) share similar lifestyles and also have similar viromes. Hypothesis about the origin and cross-kingdom transmission events of viruses have been raised and are supported by phylogenetic analysis and by the discovery of natural exchange of viruses between different hosts during virus-fungus coinfection in planta. In this review we make a compilation of the current information on the genome organization, diversity and taxonomy of mycoviruses, discussing their possible origins. Our focus is in recent findings suggesting the expansion of the host range of many viral taxa previously considered to be exclusively fungal, but we also address factors affecting virus transmissibility and coexistence in single fungal or oomycete isolates, as well as the development of synthetic mycoviruses and their use in investigating mycovirus replication cycles and pathogenicity.","PeriodicalId":50977,"journal":{"name":"Advances in Virus Research","volume":"115 ","pages":"1-86"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9460223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Tissue optical clearing and 3D imaging of virus infections. 病毒感染的组织光学清除和3D成像。

2区医学

Advances in Virus Research Pub Date : 2023-01-01 Epub Date: 2023-07-05 DOI: 10.1016/bs.aivir.2023.06.003

Dmitry S Ushakov, Stefan Finke

引用次数: 0

Host entry factors of Rift Valley Fever Virus infection. 裂谷热病毒感染的宿主进入因素。

2区医学

Advances in Virus Research Pub Date : 2023-01-01 Epub Date: 2023-10-05 DOI: 10.1016/bs.aivir.2023.09.001

Safder S Ganaie, Daisy W Leung, Amy L Hartman, Gaya K Amarasinghe

{"title":"Host entry factors of Rift Valley Fever Virus infection.","authors":"Safder S Ganaie, Daisy W Leung, Amy L Hartman, Gaya K Amarasinghe","doi":"10.1016/bs.aivir.2023.09.001","DOIUrl":"10.1016/bs.aivir.2023.09.001","url":null,"abstract":"Rift Valley Fever Virus (RVFV) is a negative sense segmented RNA virus that can cause severe hemorrhagic fever. The tri-segmented virus genome encodes for six (6) multifunctional proteins that engage host factors at a variety of different stages in the replication cycle. The S segment encodes nucleoprotein (N) and nonstructural protein S (NSs), the M segment encodes viral glycoproteins Gn and Gc as well as nonstructural protein M (NSm) and the L segment encodes the viral polymerase (L). Viral glycoproteins Gn and Gc are responsible for entry by binding to a number of host factors. Our recent studies identified a scavenger receptor, LDL receptor related protein 1 (Lrp1), as a potential pro-viral host factor for RVFV and related viruses, including Oropouche virus (OROV) infection. Coincidentally, several recent studies identified other LDL family proteins as viral entry factors and receptors for other viral families. Collectively, these observations suggest that highly conserved LDL family proteins may play a significant role in facilitating entry of viruses from several distinct families. Given the significant roles of viral and host factors during infection, characterization of these interactions is critical for therapeutic targeting with neutralizing antibodies and vaccines.","PeriodicalId":50977,"journal":{"name":"Advances in Virus Research","volume":"117 ","pages":"121-136"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11312830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41219529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The complex biology of human cytomegalovirus latency. 人巨细胞病毒潜伏期的复杂生物学。

2区医学

Advances in Virus Research Pub Date : 2022-01-01 Epub Date: 2022-03-07 DOI: 10.1016/bs.aivir.2022.01.001

Felicia Goodrum

{"title":"The complex biology of human cytomegalovirus latency.","authors":"Felicia Goodrum","doi":"10.1016/bs.aivir.2022.01.001","DOIUrl":"10.1016/bs.aivir.2022.01.001","url":null,"abstract":"While many viral infections are limited and eventually resolved by the host immune response or by death of the host, other viruses establish long-term relationships with the host by way of a persistent infection, that range from chronic viruses that may be eventually cleared to those that establish life-long persistent or latent infection. Viruses infecting hosts from bacteria to humans establish quiescent infections that must be reactivated to produce progeny. For mammalian viruses, most notably herpesviruses, this quiescent maintenance of viral genomes in the absence of virus replication is referred to as latency. The latent strategy allows the virus to persist quiescently within a single host until conditions indicate a need to reactivate to reach a new host or, to re-seed a reservoir within the host. Here, I review common themes in viral strategies to regulate the latent cycle and reactivate from it ranging from bacteriophage to herpesviruses with a focus on human cytomegalovirus (HCMV). Themes central to herpesvirus latency include, epigenetic repression of viral gene expression and mechanisms to regulate host signaling and survival. Critical to the success of a latent program are mechanisms by which the virus can \"sense\" fluctuations in host biology (within the host) or environment (outside the host) and make appropriate \"decisions\" to maintain latency or re-initiate the replicative program. The signals or environments that indicate the establishment of a latent state, the very nature of the latent state, as well as the signals driving reactivation have been topics of intense study from bacteriophage to human viruses, as these questions encompass the height of complexity in virus-host interactions-where the host and the virus coexist.","PeriodicalId":50977,"journal":{"name":"Advances in Virus Research","volume":" ","pages":"31-85"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40509355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enteroviruses: The role of receptors in viral pathogenesis. 肠病毒:受体在病毒发病机制中的作用。

2区医学

Advances in Virus Research Pub Date : 2022-01-01 DOI: 10.1016/bs.aivir.2022.09.002

Emma Heckenberg, Justin T Steppe, Carolyn B Coyne

引用次数: 2