PhytoFrontiers™最新文献_第2页

High-Quality Complete Genome Sequence of Xanthomonas phaseoli pv. dieffenbachiae Outbreak Strain D182: The Causative Agent of Anthurium Bacterial Blight in Hawai’i 夏威夷红掌细菌性枯萎病的病原体夏威夷红掌细菌性枯萎病的病原体夏威夷红掌细菌性枯萎病的病原体夏威夷红掌细菌性枯萎病的病原体夏威夷红掌细菌性疫病的病原体

PhytoFrontiers™ Pub Date : 2024-05-21 DOI: 10.1094/phytofr-02-24-0006-a

S. Dobhal, Shu-Cheng Chuang, Dario Arizala, Lisa M. Keith, Anne M. Alvarez, Mohammad Arif

引用次数: 0

Transcriptomic analysis of fludioxonil resistance mechanisms in Botrytis cinerea 灰霉病菌对氟虫腈抗性机制的转录组分析

PhytoFrontiers™ Pub Date : 2024-05-15 DOI: 10.1094/phytofr-11-23-0151-r

Maria Camila Buitrago Acosta, Nicole T. Lukasko, Mary K. Hausbeck, Timothy D. Miles, Rachel P. Naegele

{"title":"Transcriptomic analysis of fludioxonil resistance mechanisms in Botrytis cinerea","authors":"Maria Camila Buitrago Acosta, Nicole T. Lukasko, Mary K. Hausbeck, Timothy D. Miles, Rachel P. Naegele","doi":"10.1094/phytofr-11-23-0151-r","DOIUrl":"https://doi.org/10.1094/phytofr-11-23-0151-r","url":null,"abstract":"Botrytis cinerea is a destructive plant pathogen that infects a wide range of economically important crops. Limiting pathogen infection during production and post-harvest is largely dependent on fungicide applications; fungicide resistant isolates of B. cinerea have been recovered from various hosts. Resistance of B. cinerea to fludioxonil has been associated with overexpression of transporter genes (BcatrB and mfsM2) and mutations on histidine kinase proteins (Bos1, Bchhk2, Bchhk17). To identify possible mechanisms associated with fludioxonil resistance, genomic expression of three sensitive and three low-resistant isolates were studied. Overexpression of BcatrB was observed when comparing low-resistant and sensitive isolates, but was not specific to the fludioxonil treatment. Seven amino acid substitutions and one deletion were identified in the transcription factor Bcmrr1 in low-resistant isolates, associated with overexpression of BcatrB. The L497 deletion, previously associated with highly resistance isolates (MDR1h), was observed in two low-resistant isolates. Other differentially expressed genes associated with transmembrane transport, oxidoreductase activity and lipid metabolic processes could be key in understanding the fungicidal mechanism(s) of fludioxonil. Expression profiles were isolate-specific. Following fludioxonil exposure, two sensitive isolates of B. cinerea sensu stricto showed a change in gene expression levels associated with cell membrane and peroxidase activity. In one low-resistant isolate of B. cinerea group S, fludioxonil exposure resulted in the overexpression of stress response genes and MFS transporter Bcstl1; one sensitive and two low-resistant isolates showed no significant changes in gene expression profiles. This work provides insight into the effect of fludioxonil on B. cinerea and potential fungicide resistance mechanisms.","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":"18 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140976039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial Genome Resource of the Cottony Ash Psyllid, a Host of a Newly Identified ‘Candidatus Liberibacter’ bacterium 新发现的 "自由杆菌 "宿主棉灰蚜的线粒体基因组资源

PhytoFrontiers™ Pub Date : 2024-05-09 DOI: 10.1094/phytofr-11-23-0152-a

Simin Sabaghian, Zachary Gorman, Sean M. Prager, Jianchi Chen, Christopher M. Wallis

引用次数: 0

UVC (254 nm) and Far UVC (222 nm) Irradiation Affects In vitro Growth of Colletotrichum sp. Isolates and Their Infection of Detached Strawberry Leaves 紫外线（254 纳米）和远紫外线（222 纳米）辐照对草莓脱落叶片上 Colletotrichum sp. Isolates 的体外生长及其感染的影响

PhytoFrontiers™ Pub Date : 2024-05-09 DOI: 10.1094/phytofr-03-24-0016-r

Barbara J. Smith, Blair J. Sampson, Warren E. Copes, Fumiomi Takeda, Wojciech J. Janisiewicz, Eric T. Stafne, H. Sakhanokho, Jennifer B. Carroll

{"title":"UVC (254 nm) and Far UVC (222 nm) Irradiation Affects In vitro Growth of Colletotrichum sp. Isolates and Their Infection of Detached Strawberry Leaves","authors":"Barbara J. Smith, Blair J. Sampson, Warren E. Copes, Fumiomi Takeda, Wojciech J. Janisiewicz, Eric T. Stafne, H. Sakhanokho, Jennifer B. Carroll","doi":"10.1094/phytofr-03-24-0016-r","DOIUrl":"https://doi.org/10.1094/phytofr-03-24-0016-r","url":null,"abstract":"Conventional UVC at 254 nm (UVC) and Far UVC at 222 nm (Far UVC) was evaluated as alternatives to fungicides for disease control. Conidia of 13 isolates of strawberry anthracnose pathogens (five Colletotrichum species in two complexes: C. acutatum and C. gloeosporioides) were uniformly dispersed onto agar and irradiated with UVC and Far UVC doses ranging from 52 to 1248 J·m-2. After 48 h incubation, a UVC dose of 1248 J·m-2 and Far UVC doses from 234 to 935 J·m-2 reduced Colletotrichum colony counts to <10 colonies/petri dish. Cultures exposed to UVC light followed by 4 h of darkness had lower colony counts than cultures incubated under continuous light. This dark incubation period was not required for Far UVC to obtain optimal lethality, indicating Far UVC irradiation can be applied during the day or night and achieve similar fungal kill. Inoculation of detached leaves of three anthracnose susceptible strawberry cultivars with conidial suspensions of Colletotrichum spp. revealed that UV irradiation can affect development of anthracnose symptoms. Leaves receiving UVC doses of 312 and 624 J·m-2 or a Far UVC dose of 467 J·m-2 reduced anthracnose infection with little or no plant injury. A UVC dose of 1248 J·m-2 and Far UVC doses ≥ 467 J·m-2 inflicted varying degrees of plant injury. Supplemental disease control intended to reduce the number of fungicidal applications could be developed with moderate doses of UVC and Far UVC irradiation, while slowing the evolution of pesticide-resistant strains.","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140995102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivars and Production Environments Shape Shoot Endophyte Profiles of Boxwood with Different Blight Resistance 栽培品种和生产环境塑造了具有不同抗枯萎病能力的黄杨木的嫩枝内生菌特征

PhytoFrontiers™ Pub Date : 2024-05-09 DOI: 10.1094/phytofr-03-24-0023-r

Xiaoping Li, Jerry E. Weiland, Mana Ohkura, D. Luster, M. Daughtrey, Fred E. Gouker, Gloria Chen, P. Kong, Chuanxue Hong

{"title":"Cultivars and Production Environments Shape Shoot Endophyte Profiles of Boxwood with Different Blight Resistance","authors":"Xiaoping Li, Jerry E. Weiland, Mana Ohkura, D. Luster, M. Daughtrey, Fred E. Gouker, Gloria Chen, P. Kong, Chuanxue Hong","doi":"10.1094/phytofr-03-24-0023-r","DOIUrl":"https://doi.org/10.1094/phytofr-03-24-0023-r","url":null,"abstract":"Phyllosphere colonizers, including bacteria and fungi, are critical for plant growth and health. Yet, how they are affected simultaneously by host plant cultivar, local environment and agricultural practices was not well understood. We used boxwood, an iconic landscape plant and a major evergreen shrub crop in the U.S. nursery industry, as a model plant, and sequenced 16S rRNA and ITS amplicons to examine the assemblages of endophytic bacteria and fungi in the shoots of four cultivars representing three levels of boxwood blight resistance under two distinct climates and production systems in Oregon and Virginia. Cultivar and local environment were the two main drivers shaping the composition and structure of the boxwood endophytic microbial community, particularly the fungal community. Three bacterial and seven fungal genera were consistently identified with high prevalence and abundance as the core taxa from four cultivars and two locations across three sampling times. The microbial composition varied among the levels of boxwood blight resistance and taxa specific to the tolerant cultivar were fewer compared to the susceptible one. Identification of these microbial indicators, along with the core taxa, is foundational for developing a microbiome-based plant breeding program and a systems approach to improve boxwood health and production under a changing climate.","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140996555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole genome sequence resources for Pseudomonas amygdali pv. sesami and Xanthomonas arboricola isolated from sesame (Sesamum indicum) in North Carolina in 2022 2022 年从北卡罗来纳州芝麻中分离出的 Pseudomonas amygdali pv. sesami 和 Xanthomonas arboricola 的全基因组序列资源

PhytoFrontiers™ Pub Date : 2024-05-09 DOI: 10.1094/phytofr-11-23-0148-a

Katherine M. D'Amico-Willman, Kimberly M Montalban, Prasanna Joglekar, Izabela Moura Duin, David F. Ritchie, J. Fagen, Alejandra I Huerta

引用次数: 0

Environmental Influences on the Drying Rate of Spray Applied Disinfestants from Horticultural Production Surfaces 环境对园艺生产表面喷洒消毒剂干燥速度的影响

PhytoFrontiers™ Pub Date : 2024-05-09 DOI: 10.1094/phytofr-03-24-0019-r

Warren E. Copes, Quentin Read, Barbara J. Smith

{"title":"Environmental Influences on the Drying Rate of Spray Applied Disinfestants from Horticultural Production Surfaces","authors":"Warren E. Copes, Quentin Read, Barbara J. Smith","doi":"10.1094/phytofr-03-24-0019-r","DOIUrl":"https://doi.org/10.1094/phytofr-03-24-0019-r","url":null,"abstract":"Drying rates of disinfestants commonly applied to horticultural plant production surfaces were evaluated under cool to hot weather and under laboratory conditions to characterize the range of drying times and how this relates to contact times specified on product labels. Drying rates of six disinfestants [isopropyl alcohol (IPA), two quaternary ammonium compounds (QACs), two peroxygen compounds (PXs), sodium hypochlorite (bleach)] and water were evaluated when applied to six substrate materials (concrete, galvanized metal, polypropylene ground fabric, polyethylene plastic sheet, pressure-treated pine, and twin-wall clear polycarbonate) based on the weather variables of solar radiation, temperature, and relative humidity. Differences were evident at the point of application. Disinfestants with low (IPA, both QACs and one PX) and high (bleach, one PX and water) surface tension provided approximately 100% and 60% coverage, respectively, when applied to horizontal, non-porous solid materials. Disinfestants applied to horizontal porous materials (concrete, fabric and wood) persisted on the surface for a mean of only 9 to 113 sec because solutions were actively drawn into the substrates internal structure. Disinfestants applied to vertical twin-wall greenhouse material flowed off, while retaining only a maximum beaded wetness coverage of 14%. A Bayesian analysis procedure was used to model drying effects of disinfestants correlated with substrate and weather variables based on posterior marginal and prediction trends. Generally, the fastest drying rate occurred in the first 2.5 min, and approximately 50% of coverage was retained by 5 min. The evaporative process was variable with distinct interactions occurring among the experimental variables.","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140996572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host and Shelter Plants for the Beet Leafhopper which Vectors Curly Top Viruses and Phytoplasmas in Southern Idaho 爱达荷州南部甜菜叶蝉的寄主植物和庇护植物，甜菜叶蝉是卷曲病毒和植物病原体的传播媒介

PhytoFrontiers™ Pub Date : 2024-05-09 DOI: 10.1094/phytofr-03-24-0022-r

C. A. Strausbaugh, E. Wenninger, Laurie K. Jackson, Eric Vincill

{"title":"Host and Shelter Plants for the Beet Leafhopper which Vectors Curly Top Viruses and Phytoplasmas in Southern Idaho","authors":"C. A. Strausbaugh, E. Wenninger, Laurie K. Jackson, Eric Vincill","doi":"10.1094/phytofr-03-24-0022-r","DOIUrl":"https://doi.org/10.1094/phytofr-03-24-0022-r","url":null,"abstract":"Weeds and crop plants not only serve as reproductive hosts and transitory or shelter plants for the beet leafhopper (BLH; Circulifer tenellus) but also as sources of plant pathogens that can then be vectored by the BLH. Thus, the plants that the BLH are feeding on and infecting is of interest and may be changing over time. Therefore, BLH samples from a recent survey were investigated through DNA barcoding via the rbcL and matK chloroplast gene regions to determine what the BLHs had been feeding on prior to capture on yellow sticky cards in southern Idaho during 2020 and 2021. In June both years, the first generation of BLHs predominately fed on Pinus spp. (59 to 76% of samples) which were likely in mountainous areas and dispersed approximately 48 to 80 km to crop and sagebrush steppe locations. During July to September, the BLH predominantly fed on Salsola spp. (Russian thistle; 61 to 66% of samples) and Bassia scoparia (Kochia; 15% of samples). Both years the BLHs that fed on pine had the highest percentage (55 and 75%, respectively) of samples with beet curly top virus based on primers that can detect both the Worland and Colorado strains. Both years, BLH that had fed on Russian thistle and alfalfa had the highest percentage of samples with Spinach curly top Arizona virus. These data will be utilized in the development of future curly top management plans.","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":" 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140997284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Soil Microbiomes of American Ginseng (Panax quinquefolius) Cultivated in North America Differ by Location But Not Always by Disease Status 北美栽培的花旗参的土壤微生物组因地点而异，但并非总是因病害状况而异

PhytoFrontiers™ Pub Date : 2024-04-22 DOI: 10.1094/phytofr-09-23-0119-r

Isabella J. Maggard, Kayla B. Deel, Ying Gao, A. B. Cahoon

{"title":"The Soil Microbiomes of American Ginseng (Panax quinquefolius) Cultivated in North America Differ by Location But Not Always by Disease Status","authors":"Isabella J. Maggard, Kayla B. Deel, Ying Gao, A. B. Cahoon","doi":"10.1094/phytofr-09-23-0119-r","DOIUrl":"https://doi.org/10.1094/phytofr-09-23-0119-r","url":null,"abstract":"One of the greatest challenges faced by ginseng producers is control of fungal pathogens which adversely affects harvest yields and is a contributing factor to replant failure syndrome. The goal of this project was to survey the soil microbiome of healthy versus diseased American Ginseng grown in North America. Soil associated with healthy and diseased plants were collected from commercial farms in Garett County, Maryland and Marathon County, Wisconsin. Environmental DNA metabarcoding was used to compare the bacterial and fungal microbiomes of healthy versus diseased plants from both locations. The most common bacteria occurred within the phylum Proteobacteria, Firmicutes, and Bacteroidota and the most common fungi occurred within Ascomycota, Basidiomytoca, Mortierellomycota and Glomeromycota. Overall Alpha- and Beta- diversities were statistically significant between the two locations. Community analyses (beta-diversity) of the Maryland site revealed a significant difference in the bacterial composition of soils associated with healthy versus diseased plants but not fungi. Conversely, there was a significantly different assemblage of fungi in healthy versus diseased associated soils from the Wisconsin site, but not for bacteria. Our results when compared to previous metabarcoding studies suggest there is little congruence between the soil microbiomes associated with cultivated ginseng and that we are still in the early stages of ginseng soil rhizosphere exploration.","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140677822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering transcriptomic and metabolomic wood responses to grapevine trunk diseases-associated fungi 解密木材对葡萄树干病害相关真菌的转录组和代谢组反应

PhytoFrontiers™ Pub Date : 2024-04-17 DOI: 10.1094/phytofr-10-23-0132-r

Ana Romeo-Oliván, J. Chervin, Coralie Breton, Virginie Puech-Pagès, Sylvie Fournier, Guillaume Marti, Olivier Rodrigues, Jean Daydé, Bernard Dumas, Alban Jacques

{"title":"Deciphering transcriptomic and metabolomic wood responses to grapevine trunk diseases-associated fungi","authors":"Ana Romeo-Oliván, J. Chervin, Coralie Breton, Virginie Puech-Pagès, Sylvie Fournier, Guillaume Marti, Olivier Rodrigues, Jean Daydé, Bernard Dumas, Alban Jacques","doi":"10.1094/phytofr-10-23-0132-r","DOIUrl":"https://doi.org/10.1094/phytofr-10-23-0132-r","url":null,"abstract":"Esca is one of the main grapevine trunk diseases affecting vineyards worldwide. Phaeoacremonium minimum and Phaeomoniella chlamydospora are thought to be two of the main causal agents of this disease. However, the molecular mechanisms underlying plant defense responses in the grapevine trunk against esca-associated pathogens are poorly understood. To provide a first glimpse on the trunk responses to P. minimum and P. chlamydospora, transcriptomic and metabolomic analyses were performed to compare and contrast host responses to these pathogens. Transcriptomic analysis revealed different gene expression reprogramming in the trunk in response to each fungus. Main significant differences were found among genes associated with Secondary Metabolism, Signaling and Hormone Signaling. An untargeted liquid chromatography–high resolution mass spectrometry metabolomic approach performed 3 weeks after inoculation was used and dereplication mainly highlighted flavonoids and stilbenes as plant defense metabolites in the infected trunk. Some metabolites were overproduced with both fungi, but specific responses were also observed. Particularly, a lipophilic flavonoid cluster was emphasized after P. minimum inoculation. The assessment of fungal infection 6 wpi showed a higher number of copies of P. minimum than P. chlamydospora. This dissimilarity in the level of colonization could be linked somehow to the metabolomic responses observed. Our results reveal both different gene expression reprogramming and metabolomic specific signatures depending on the wood pathogen. Altogether, these observations suggest that grapevine trunk can differently perceive and respond to P. minimum and P. chlamydospora.","PeriodicalId":508090,"journal":{"name":"PhytoFrontiers™","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140693671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0