High-Quality Complete Genome Sequence of Xanthomonas phaseoli pv. dieffenbachiae Outbreak Strain D182: The Causative Agent of Anthurium Bacterial Blight in Hawai’i

S. Dobhal, Shu-Cheng Chuang, Dario Arizala, Lisa M. Keith, Anne M. Alvarez, Mohammad Arif
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Abstract

Xanthomonas phaseoli pv. dieffenbachiae (Xpd), the causal agent of anthurium blight, is classified as an A2 quarantine organism on the EPPO list due to its devastating impact on the anthurium industry. In this study, we sequenced strain D182, representative of the Hawaiian anthurium blight outbreak (1981-1986), using PacBio RS II SMRT technology. High-quality de novo assembly of 5,217,888 bp (65% GC) was generated with a mean coverage of 351x using HGAP v4. Strain D182 harbors one plasmid (73.5 kb, GC – 60.8%). ANI and dDDH values of 99.86% and 98.9% respectively, showed close phylogenetic relationships with Xpd strain LMG 695PT. The genome information will be useful in providing insights into the genomic biology, virulence mechanisms, and evolutionary relationships of Xpd and other strains associated with anthurium blight outbreaks worldwide.
夏威夷红掌细菌性枯萎病的病原体夏威夷红掌细菌性枯萎病的病原体夏威夷红掌细菌性枯萎病的病原体夏威夷红掌细菌性枯萎病的病原体夏威夷红掌细菌性疫病的病原体
黄单胞菌(Xanthomonas phaseoli pv. dieffenbachiae,Xpd)是红掌疫病的病原菌,由于其对红掌产业的破坏性影响,被列为 EPPO 名单中的 A2 检疫生物。在本研究中,我们利用 PacBio RS II SMRT 技术对夏威夷红掌疫病爆发(1981-1986 年)的代表菌株 D182 进行了测序。菌株 D182 含有一个质粒(73.5 kb,GC - 60.8%)。ANI 和 dDDH 值分别为 99.86% 和 98.9%,表明与 Xpd 菌株 LMG 695PT 的系统发育关系密切。该基因组信息将有助于深入了解 Xpd 及其他与全球红掌疫病暴发相关的菌株的基因组生物学、毒力机制和进化关系。
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