Simin Sabaghian, Zachary Gorman, Sean M. Prager, Jianchi Chen, Christopher M. Wallis
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引用次数: 0
摘要
白蜡木虱(CAP,Phyllopsis discrepans)是入侵北美白蜡树的一种重要害虫,它在北美已建立了种群,并且是新发现的 "Candidatus Liberibacter solanacearum "菌株的宿主。然而,人们对其引入种群的多样性知之甚少。在这项研究中,我们从加拿大萨斯喀彻温省萨斯卡通的一个采集点获得了 CAP 线粒体基因组(有丝分裂基因组)序列。CAP 线粒体基因组是一个 18,824 bp 的环状 DNA,编码 13 个蛋白质编码基因、21 个转移 RNA 基因和 2 个核糖体 RNA 基因。以 CAP 有丝分裂基因组为查询对象,对 GenBank 序列数据库进行 BLAST 搜索,结果表明 Euphyllura phillyreae 的有丝分裂基因组(15,202 bp)最为相似(查询覆盖率 = 77%;同一性百分比 = 78.90%)。CAP 的有丝分裂基因组与其他已知的烟粉虱有丝分裂基因组有明显不同,因为它存在一个 4,357 bp 的控制区(CR)。有丝分裂基因组序列将进一步加深对 CAP 基因组的了解。
Mitochondrial Genome Resource of the Cottony Ash Psyllid, a Host of a Newly Identified ‘Candidatus Liberibacter’ bacterium
Cottony ash psyllid (CAP, Phyllopsis discrepans) is an important, invasive insect pest of ash trees in North America where it has established populations and is the host of a newly identified strain of 'Candidatus Liberibacter solanacearum'. However, not much is known about the diversity of its introduced population. In this study, a CAP mitochondrial genome (mitogenome) sequence was obtained from a collection in Saskatoon, Saskatchewan, Canada. The CAP mitogenome is a circular DNA of 18,824 bp, encoding 13 protein-coding genes, 21 transfer RNA genes, and 2 ribosomal RNA genes. BLAST search using the CAP mitogenome as query against GenBank sequence database showed the mitogenome of Euphyllura phillyreae (15,202 bp) was the most similar (Query Coverage = 77%; Percentage Identity = 78.90%). The CAP mitogenome is significantly different from other known psyllid mitogenomes with the presence of a 4,357 bp control region (CR). The mitogenome sequence will further genomic understand of CAP.