Annual Review of Biophysics最新文献

Size Matters: A Biophysical Perspective on Biomolecular Condensates in Bacteria. 大小问题：细菌生物分子凝聚物的生物物理学观点。

IF 13.7 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2025-12-12 DOI: 10.1146/annurev-biophys-021424-010232

Lydia Hodgins, Baljyot Singh Parmar, Rodrigo Reyes-Lamothe, Stephanie C Weber

引用次数: 0

Structures of Photosynthetic Supramolecular Complexes. 光合作用超分子复合物的结构。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2026-02-05 DOI: 10.1146/annurev-biophys-021424-011156

Zhenfeng Liu, Xin You, Mei Li, Sen-Fang Sui

{"title":"Structures of Photosynthetic Supramolecular Complexes.","authors":"Zhenfeng Liu, Xin You, Mei Li, Sen-Fang Sui","doi":"10.1146/annurev-biophys-021424-011156","DOIUrl":"10.1146/annurev-biophys-021424-011156","url":null,"abstract":"Photosynthesis, the biological process of converting light energy into chemical energy, involves light harvesting, charge separation and electron transport, proton translocation, ATP synthesis, and carbon fixation, among other processes. Adjacent photosynthetic complexes may assemble into supramolecular complexes to couple and regulate their functions. Here, we review the progress of structural biology studies of photosynthetic supramolecular complexes, such as those that have light-harvesting complexes assembled with photosystem II (PSII) or photosystem I (PSI), both PSII and PSI, or bacterial reaction center complexes. The intricate architectures of the NADH dehydrogenase-like (NDH) complex and PSI-NDH supercomplex, revealed through cryo-electron microscopy studies, provide crucial frameworks for understanding the molecular mechanisms of cyclic electron flow in cyanobacteria and plants. Furthermore, structural studies have also yielded detailed insights into the assembly and repair of PSII, regulation of ATP synthase, and carbon fixation. The review concludes with a summary of the emerging directions of structural biology studies of photosynthetic supramolecular complexes.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":" ","pages":"415-441"},"PeriodicalIF":12.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146126574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Cell Proteomic Technologies: Tools in the Quest for Principles. 单细胞蛋白质组学技术：寻求原理的工具。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2026-01-23 DOI: 10.1146/annurev-biophys-021424-011450

Nikolai Slavov

引用次数: 0

Mapping Protein Conformational Landscapes with High-Pressure NMR. 用高压核磁共振绘制蛋白质构象景观。

IF 13.7 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2025-11-18 DOI: 10.1146/annurev-biophys-022224-105324

Catherine Royer

引用次数: 0

Rigidity and Mechanical Response in Biological Structures. 生物结构的刚度和力学响应。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2026-02-10 DOI: 10.1146/annurev-biophys-021424-014456

Kelly Aspinwall, Tyler Hain, M Lisa Manning

引用次数: 0

Nonequilibrium Thermodynamics for Biochemical Networks: Energetic Principles of Cellular Function. 生物化学网络的非平衡热力学：细胞功能的能量原理。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2026-01-28 DOI: 10.1146/annurev-biophys-021424-112247

Yuhai Tu

{"title":"Nonequilibrium Thermodynamics for Biochemical Networks: Energetic Principles of Cellular Function.","authors":"Yuhai Tu","doi":"10.1146/annurev-biophys-021424-112247","DOIUrl":"10.1146/annurev-biophys-021424-112247","url":null,"abstract":"I review recent advances in nonequilibrium thermodynamics of biochemical networks, organized around two central questions. First, why is free energy dissipation essential for enabling or enhancing biological function? Second, how do energetic costs constrain functional performance? Using several representative systems-beginning with the classical kinetic proofreading mechanism and extending to more recent examples such as accurate sensory adaptation, ultrasensitive responses, and synchronization of biochemical oscillators-I show that this framework not only provides new insights into the molecular mechanisms underlying these diverse processes but also reveals the general thermodynamic principles that govern their biological functions. I highlight the characteristic signatures of nonequilibrium behavior and the emergence of fundamental energy-performance trade-offs. This review strives to present the framework pedagogically and with sufficient technical detail to enable theory-inclined biophysicists to apply it to their own systems of interest. I conclude by proposing a nonequilibrium thermodynamic law for living systems and outlining promising directions for extending this theoretical approach to an even broader range of biological phenomena.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":"55 1","pages":"225-252"},"PeriodicalIF":12.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147845691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanopore Single-Molecule Chemistry. 纳米孔单分子化学。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2026-01-23 DOI: 10.1146/annurev-biophys-021424-125106

Yao Liu, Xinmeng Gao, Shuo Huang

{"title":"Nanopore Single-Molecule Chemistry.","authors":"Yao Liu, Xinmeng Gao, Shuo Huang","doi":"10.1146/annurev-biophys-021424-125106","DOIUrl":"10.1146/annurev-biophys-021424-125106","url":null,"abstract":"Nanopores have become transformative tools in single-molecule chemical analysis, enabling detailed interrogation of molecular interactions and reaction dynamics. These advancements have revolutionized the characterization of chemical kinetics and stereospecificity, broadening nanopore applications. This review evaluates the principles of nanopore single-molecule chemistry, highlighting breakthroughs in chemically reactive nanopore construction via site-specific mutagenesis, semisynthetic engineering, and orthogonal modifications. Notably, we highlight the innovative strategies enabling precise subunit stoichiometry control to ensure single-molecule reactions, and the integration of machine learning for high-fidelity ionic current analysis. These developments position nanopores as versatile tools for intricate molecular detection in fundamental and applied research. Looking forward, nanopore single-molecule chemistry promises an impact on diagnostics, environmental monitoring, and precision medicine. Integration of molecular dynamics simulations, artificial intelligence-driven protein design frameworks, and microsystems technology may expand detectable species, enhancing robustness and lowering detection limits. Such advancements will deepen our understanding of chemical transformations and support meaningful real-world applications of nanopore technologies.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":" ","pages":"277-300"},"PeriodicalIF":12.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146042149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systems Biology of Aging, Metabolism, and Mitochondria. 衰老、代谢和线粒体的系统生物学。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2026-02-10 DOI: 10.1146/annurev-biophys-021424-011852

Miguel Antonio Aon, Sonia Cortassa

{"title":"Systems Biology of Aging, Metabolism, and Mitochondria.","authors":"Miguel Antonio Aon, Sonia Cortassa","doi":"10.1146/annurev-biophys-021424-011852","DOIUrl":"10.1146/annurev-biophys-021424-011852","url":null,"abstract":"Since the beginning of this century, the emergence of systems biology, driven by technological, informatic, and theoretical advances, has led to an unprecedented generation of data and information about biological systems at multiple levels of organization. We now have access not only to components of living systems but also to some of the underlying principles governing their organization within networks. This review focuses on the systems biology of aging, metabolism, and mitochondria, along with the integration of experimental and computational systems biology approaches as applied to multilayered biological networks, spanning from the molecular-subcellular to the whole organism. Sections 2 and 3 provide an overview of the insights gained from systems biology and multi-omics approaches as applied to aging and metabolism. Using the spatiotemporal dynamics of biological networks as a unifying thread, Sections 4 and 5 explore how systems biology and current methods can leverage the understanding of complex biological phenomena through integrated experimental-computational strategies, utilizing iterative, verification-validation loops between experiments and models. Section 6 concludes by highlighting the autonomously dynamic, self-organizing, and self-regulating integrative nature of living systems and the need to address these properties at the emerging convergence of biology, medicine, physics, and powerful computational technologies that include artificial intelligence.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":" ","pages":"443-467"},"PeriodicalIF":12.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146159169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imaging Dynamic Subcellular Organization at High Spatiotemporal Resolution. 高时空分辨率动态亚细胞组织成像。

IF 13.7 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2026-01-02 DOI: 10.1146/annurev-biophys-021424-011728

Francesca W van Tartwijk, Liuba Dvinskikh, Edward N Ward, Meng Lu, Clemens F Kaminski

引用次数: 0

Ligand Binding Dynamics of Ion Channels and GPCRs Using Single-Molecule Fluorescence. 利用单分子荧光研究离子通道和gpcr的配体结合动力学。

IF 12.9 1区生物学

Annual Review of Biophysics Pub Date : 2026-05-01 Epub Date: 2026-02-05 DOI: 10.1146/annurev-biophys-030722-113838

Susovan Roy Chowdhury, Randall H Goldsmith, Baron Chanda

{"title":"Ligand Binding Dynamics of Ion Channels and GPCRs Using Single-Molecule Fluorescence.","authors":"Susovan Roy Chowdhury, Randall H Goldsmith, Baron Chanda","doi":"10.1146/annurev-biophys-030722-113838","DOIUrl":"10.1146/annurev-biophys-030722-113838","url":null,"abstract":"Chemical signaling underlies many biological processes, and membrane receptors such as G protein-coupled receptors and ligand-gated ion channels represent two of the most pharmacologically important protein families. Advances in single-molecule fluorescence techniques have transformed our understanding of molecular mechanisms, including protein folding, transcription, and ligand binding. Unlike ensemble measurements, which average over populations and obscure molecular heterogeneity, single-molecule approaches enable direct observation of individual events, revealing rare conformational states and distinguishing between mechanisms that are indistinguishable at the ensemble level. This review highlights how single-molecule fluorescence resonance energy transfer (smFRET) and single-molecule fluorescence ligand binding (smFLiB) provide complementary insights into ligand-dependent receptor activation and allosteric coupling. smFRET offers structural information by tracking conformational transitions, but limited observation times can hinder detection of slow or infrequent events. In contrast, smFLiB allows long-duration monitoring of ligand-receptor interactions throughout the activation pathway, though with less direct information about structural rearrangements. Through selected case studies, we illustrate how these techniques have been applied to dissect the complexity of ligand-receptor interactions with unprecedented resolution. These advances hold promise for guiding the rational design of more selective and effective therapeutics targeting membrane proteins.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":" ","pages":"343-364"},"PeriodicalIF":12.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13485122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146127394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0