Annual Review of Biophysics最新文献_第10页

Temperature, Dynamics, and Enzyme-Catalyzed Reaction Rates. 温度、动力学和酶催化反应速率。

IF 12.4 1区生物学

Annual Review of Biophysics Pub Date : 2020-05-06 DOI: 10.1146/annurev-biophys-121219-081520

Vickery L Arcus, Adrian J Mulholland

{"title":"Temperature, Dynamics, and Enzyme-Catalyzed Reaction Rates.","authors":"Vickery L Arcus, Adrian J Mulholland","doi":"10.1146/annurev-biophys-121219-081520","DOIUrl":"https://doi.org/10.1146/annurev-biophys-121219-081520","url":null,"abstract":"We review the adaptations of enzyme activity to different temperatures. Psychrophilic (cold-adapted) enzymes show significantly different activation parameters (lower activation enthalpies and entropies) than their mesophilic counterparts. Furthermore, there is increasing evidence that the temperature dependence of many enzyme-catalyzed reactions is more complex than is widely believed. Many enzymes show curvature in plots of activity versus temperature that is not accounted for by denaturation or unfolding. This is explained by macromolecular rate theory: A negative activation heat capacity for the rate-limiting chemical step leads directly to predictions of temperature optima; both entropy and enthalpy are temperature dependent. Fluctuations in the transition state ensemble are reduced compared to the ground state. We show how investigations combining experiment with molecular simulation are revealing fundamental details of enzyme thermoadaptation that are relevant for understanding aspects of enzyme evolution. Simulations can calculate relevant thermodynamic properties (such as activation enthalpies, entropies, and heat capacities) and reveal the molecular mechanisms underlying experimentally observed behavior. Expected final online publication date for the Annual Review of Biophysics, Volume 49 is May 6, 2020. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":"49 ","pages":"163-180"},"PeriodicalIF":12.4,"publicationDate":"2020-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1146/annurev-biophys-121219-081520","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9146131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

RNA-Mediated Virus Assembly: Mechanisms and Consequences for Viral Evolution and Therapy. rna介导的病毒组装:病毒进化和治疗的机制和后果。

IF 12.4 1区生物学

Annual Review of Biophysics Pub Date : 2019-05-06 DOI: 10.1146/annurev-biophys-052118-115611

Reidun Twarock, Peter G Stockley

{"title":"RNA-Mediated Virus Assembly: Mechanisms and Consequences for Viral Evolution and Therapy.","authors":"Reidun Twarock, Peter G Stockley","doi":"10.1146/annurev-biophys-052118-115611","DOIUrl":"https://doi.org/10.1146/annurev-biophys-052118-115611","url":null,"abstract":"Viruses, entities composed of nucleic acids, proteins, and in some cases lipids lack the ability to replicate outside their target cells. Their components self-assemble at the nanoscale with exquisite precision-a key to their biological success in infection. Recent advances in structure determination and the development of biophysical tools such as single-molecule spectroscopy and noncovalent mass spectrometry allow unprecedented access to the detailed assembly mechanisms of simple virions. Coupling these techniques with mathematical modeling and bioinformatics has uncovered a previously unsuspected role for genomic RNA in regulating formation of viral capsids, revealing multiple, dispersed RNA sequence/structure motifs [packaging signals (PSs)] that bind cognate coat proteins cooperatively. The PS ensemble controls assembly efficiency and accounts for the packaging specificity seen in vivo. The precise modes of action of the PSs vary between viral families, but this common principle applies across many viral families, including major human pathogens. These insights open up the opportunity to block or repurpose PS function in assembly for both novel antiviral therapy and gene/drug/vaccine applications.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":"48 ","pages":"495-514"},"PeriodicalIF":12.4,"publicationDate":"2019-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1146/annurev-biophys-052118-115611","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9103478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 50

Hydrogel-Tissue Chemistry: Principles and Applications. 水凝胶-组织化学:原理和应用。

IF 12.4 1区生物学

Annual Review of Biophysics Pub Date : 2018-05-20 DOI: 10.1146/annurev-biophys-070317-032905

Viviana Gradinaru, Jennifer Treweek, Kristin Overton, Karl Deisseroth

{"title":"Hydrogel-Tissue Chemistry: Principles and Applications.","authors":"Viviana Gradinaru, Jennifer Treweek, Kristin Overton, Karl Deisseroth","doi":"10.1146/annurev-biophys-070317-032905","DOIUrl":"https://doi.org/10.1146/annurev-biophys-070317-032905","url":null,"abstract":"Over the past five years, a rapidly developing experimental approach has enabled high-resolution and high-content information retrieval from intact multicellular animal (metazoan) systems. New chemical and physical forms are created in the hydrogel-tissue chemistry process, and the retention and retrieval of crucial phenotypic information regarding constituent cells and molecules (and their joint interrelationships) are thereby enabled. For example, rich data sets defining both single-cell-resolution gene expression and single-cell-resolution activity during behavior can now be collected while still preserving information on three-dimensional positioning and/or brain-wide wiring of those very same neurons-even within vertebrate brains. This new approach and its variants, as applied to neuroscience, are beginning to illuminate the fundamental cellular and chemical representations of sensation, cognition, and action. More generally, reimagining metazoans as metareactants-or positionally defined three-dimensional graphs of constituent chemicals made available for ongoing functionalization, transformation, and readout-is stimulating innovation across biology and medicine.","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":"47 ","pages":"355-376"},"PeriodicalIF":12.4,"publicationDate":"2018-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1146/annurev-biophys-070317-032905","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9340255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 90

The Biophysics of 3D Cell Migration 三维细胞迁移的生物物理学

IF 12.4 1区生物学

Annual Review of Biophysics Pub Date : 2018-05-20 DOI: 10.1146/ANNUREV-BIOPHYS-070816-033854

Pei-Hsun Wu, Daniele M. Gilkes, D. Wirtz

引用次数: 34

Mechanotransduction by the Actin Cytoskeleton: Converting Mechanical Stimuli into Biochemical Signals 肌动蛋白细胞骨架的机械转导:将机械刺激转化为生化信号

IF 12.4 1区生物学

Annual Review of Biophysics Pub Date : 2018-05-20 DOI: 10.1146/ANNUREV-BIOPHYS-070816-033547

A. Harris, P. Jreij, D. Fletcher

引用次数: 82

Soft Matter in Lipid-Protein Interactions. 脂质-蛋白相互作用中的软物质。

IF 12.4 1区生物学

Annual Review of Biophysics Pub Date : 2017-05-22 DOI: 10.1146/annurev-biophys-070816-033843

Michael F Brown

引用次数: 89

Structural Insights into the Eukaryotic Transcription Initiation Machinery. 真核生物转录启动机制的结构透视。

IF 10.4 1区生物学

Annual Review of Biophysics Pub Date : 2017-05-22 DOI: 10.1146/annurev-biophys-070816-033751

Eva Nogales, Robert K Louder, Yuan He

引用次数: 0

Mechanisms of ATP-Dependent Chromatin Remodeling Motors. atp依赖性染色质重塑马达的机制。

IF 12.4 1区生物学

Annual Review of Biophysics Pub Date : 2016-07-05 DOI: 10.1146/annurev-biophys-051013-022819

Coral Y Zhou, Stephanie L Johnson, Nathan I Gamarra, Geeta J Narlikar

引用次数: 115

Regulation of Rad6/Rad18 Activity During DNA Damage Tolerance. DNA 损伤耐受过程中 Rad6/Rad18 活性的调控

IF 12.4 1区生物学

Annual Review of Biophysics Pub Date : 2015-01-01 DOI: 10.1146/annurev-biophys-060414-033841

Mark Hedglin, Stephen J Benkovic

引用次数: 0

Structural Symmetry in Membrane Proteins. 膜蛋白的结构对称性。

IF 12.4 1区生物学

Annual Review of Biophysics Pub Date : 2015-01-01 DOI: 10.1146/annurev-biophys-051013-023008

Lucy R Forrest

{"title":"Structural Symmetry in Membrane Proteins.","authors":"Lucy R Forrest","doi":"10.1146/annurev-biophys-051013-023008","DOIUrl":"https://doi.org/10.1146/annurev-biophys-051013-023008","url":null,"abstract":"Symmetry is a common feature among natural systems, including protein structures. A strong propensity toward symmetric architectures has long been recognized for water-soluble proteins, and this propensity has been rationalized from an evolutionary standpoint. Proteins residing in cellular membranes, however, have traditionally been less amenable to structural studies, and thus the prevalence and significance of symmetry in this important class of molecules is not as well understood. In the past two decades, researchers have made great strides in this area, and these advances have provided exciting insights into the range of architectures adopted by membrane proteins. These structural studies have revealed a similarly strong bias toward symmetric arrangements, which were often unexpected and which occurred despite the restrictions imposed by the membrane environment on the possible symmetry groups. Moreover, membrane proteins disproportionately contain internal structural repeats resulting from duplication and fusion of smaller segments. This article discusses the types and origins of symmetry in membrane proteins and the implications of symmetry for protein function. ","PeriodicalId":50756,"journal":{"name":"Annual Review of Biophysics","volume":"44 ","pages":"311-37"},"PeriodicalIF":12.4,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1146/annurev-biophys-051013-023008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33409763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 114