Chromosome Research最新文献_第10页

Correction to: Identification of passion fruit (Passiflora edulis) chromosomes using BAC-FISH. 更正:用BAC-FISH方法鉴定百香果(Passiflora edulis)染色体。

IF 2.6 4区生物学

Chromosome Research Pub Date : 2021-06-01 DOI: 10.1007/s10577-020-09633-2

M A Sader, Y Dias, Z P Costa, C Munhoz, H Penha, H Bergès, M L C Vieira, A Pedrosa-Harand

引用次数: 1

SOGA1 and SOGA2/MTCL1 are CLASP-interacting proteins required for faithful chromosome segregation in human cells. SOGA1和SOGA2/MTCL1是人类细胞中忠实的染色体分离所需的clasp相互作用蛋白。

IF 2.6 4区生物学

Chromosome Research Pub Date : 2021-06-01 Epub Date: 2021-02-15 DOI: 10.1007/s10577-021-09651-8

Luísa T Ferreira, Elsa Logarinho, Joana C Macedo, Ana Rita R Maia, Helder Maiato

{"title":"SOGA1 and SOGA2/MTCL1 are CLASP-interacting proteins required for faithful chromosome segregation in human cells.","authors":"Luísa T Ferreira, Elsa Logarinho, Joana C Macedo, Ana Rita R Maia, Helder Maiato","doi":"10.1007/s10577-021-09651-8","DOIUrl":"https://doi.org/10.1007/s10577-021-09651-8","url":null,"abstract":"CLASPs are key modulators of microtubule dynamics throughout the cell cycle. During mitosis, CLASPs independently associate with growing microtubule plus-ends and kinetochores and play essential roles in chromosome segregation. In a proteomic survey for human CLASP1-interacting proteins during mitosis, we have previously identified SOGA1 and SOGA2/MTCL1, whose mitotic roles remained uncharacterized. Here we performed an initial functional characterization of human SOGA1 and SOGA2/MTCL1 during mitosis. Using specific polyclonal antibodies raised against SOGA proteins, we confirmed their expression and reciprocal interaction with CLASP1 and CLASP2 during mitosis. In addition, we found that both SOGA1 and SOGA2/MTCL1 are phospho-regulated during mitosis by CDK1. Immunofluorescence analysis revealed that SOGA2/MTCL1 co-localizes with mitotic spindle microtubules and spindle poles throughout mitosis and both SOGA proteins are enriched at the midbody during mitotic exit/cytokinesis. GFP-tagging of SOGA2/MTCL1 further revealed a microtubule-independent localization at kinetochores. Live-cell imaging after siRNA-mediated knockdown of SOGA1 and SOGA2/MTCL1 showed that they are independently required for distinct aspects of chromosome segregation. Thus, SOGA1 and SOGA2/MTCL1 are bona fide CLASP-interacting proteins during mitosis required for faithful chromosome segregation in human cells.","PeriodicalId":50698,"journal":{"name":"Chromosome Research","volume":"29 2","pages":"159-173"},"PeriodicalIF":2.6,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-021-09651-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25369425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclic DNA remethylation following active demethylation at euchromatic regions in mouse embryonic stem cells. 小鼠胚胎干细胞正色区活性去甲基化后的环状DNA再甲基化。

IF 2.6 4区生物学

Chromosome Research Pub Date : 2021-06-01 Epub Date: 2020-11-17 DOI: 10.1007/s10577-020-09645-y

Musashi Kubiura-Ichimaru, Takamasa Ito, Louis Lefebvre, Masako Tada

{"title":"Cyclic DNA remethylation following active demethylation at euchromatic regions in mouse embryonic stem cells.","authors":"Musashi Kubiura-Ichimaru, Takamasa Ito, Louis Lefebvre, Masako Tada","doi":"10.1007/s10577-020-09645-y","DOIUrl":"https://doi.org/10.1007/s10577-020-09645-y","url":null,"abstract":"DNA methylation is an essential epigenetic mark that regulates normal mammalian embryonic development. DNA methylation profiles are not always static, especially during germline development. In zygotes, DNA is typically highly methylated but, during preimplantation, DNA methylation is erased globally. Then, at the start of post-implantation development in mouse embryos, DNA again becomes dramatically hypermethylated. Chromatin structure regulates the accessibility of DNA-modifying enzymes to target DNA. Beyond that, however, our understanding of the pathway by which chromatin regulation initiates changes in global DNA methylation during mouse embryonic development remains incomplete. To analyse the relationship between global regulation of DNA methylation and chromatin status, we examined 5-methylcytosine (5mC), modified by the DNA methyltransferase DNMT, and the oxidative derivative 5-hydroxymethylation (5hmC), converted from 5mC by TET-family enzymes, by means of immunofluorescence staining of mitotic chromosomes in mouse embryonic stem cells (ESCs). Our comparison of immunostaining patterns for those epigenetic modifications in wild-type, DNMT-deficient, and TET-deficient ESCs allowed us to visualise cell cycle-mediated DNA methylation changes, especially in euchromatic regions. Our findings suggest that DNA methylation patterns in undifferentiated mouse ESCs are stochastically balanced by the opposing effects of two activities: demethylation by TET and subsequent remethylation by DNMT.","PeriodicalId":50698,"journal":{"name":"Chromosome Research","volume":"29 2","pages":"145-157"},"PeriodicalIF":2.6,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-020-09645-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38615236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mitotic checkpoint defects: en route to cancer and drug resistance. 有丝分裂检查点缺陷:通往癌症和耐药性的道路。

IF 2.6 4区生物学

Chromosome Research Pub Date : 2021-06-01 Epub Date: 2021-01-06 DOI: 10.1007/s10577-020-09646-x

Sinjini Sarkar, Pranab Kumar Sahoo, Sutapa Mahata, Ranita Pal, Dipanwita Ghosh, Tanuma Mistry, Sushmita Ghosh, Tanmoy Bera, Vilas D Nasare

{"title":"Mitotic checkpoint defects: en route to cancer and drug resistance.","authors":"Sinjini Sarkar, Pranab Kumar Sahoo, Sutapa Mahata, Ranita Pal, Dipanwita Ghosh, Tanuma Mistry, Sushmita Ghosh, Tanmoy Bera, Vilas D Nasare","doi":"10.1007/s10577-020-09646-x","DOIUrl":"https://doi.org/10.1007/s10577-020-09646-x","url":null,"abstract":"Loss of mitosis regulation is a common feature of malignant cells that leads to aberrant cell division with inaccurate chromosome segregation. The mitotic checkpoint is responsible for faithful transmission of genetic material to the progeny. Defects in this checkpoint, such as mutations and changes in gene expression, lead to abnormal chromosome content or aneuploidy that may facilitate cancer development. Furthermore, a defective checkpoint response is indicated in the development of drug resistance to microtubule poisons that are used in treatment of various blood and solid cancers for several decades. Mitotic slippage and senescence are important cell fates that occur even with an active mitotic checkpoint and are held responsible for the resistance. However, contradictory findings in both the scenarios of carcinogenesis and drug resistance have aroused questions on whether mitotic checkpoint defects are truly responsible for these dismal outcomes. Here, we discuss the possible contribution of the faulty checkpoint signaling in cancer development and drug resistance, followed by the latest research on this pathway for better outcomes in cancer treatment.","PeriodicalId":50698,"journal":{"name":"Chromosome Research","volume":"29 2","pages":"131-144"},"PeriodicalIF":2.6,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-020-09646-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38790957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

CEA, CA 15-3, and miRNA expression as potential biomarkers in canine mammary tumors. CEA, ca15 -3和miRNA表达作为犬乳腺肿瘤的潜在生物标志物。

IF 2.6 4区生物学

Chromosome Research Pub Date : 2021-06-01 Epub Date: 2021-02-27 DOI: 10.1007/s10577-021-09652-7

Mohit Jain, Shailesh D Ingole, Rahul S Deshmukh, Simin V Bharucha, Anagha S Nagvekar, Rajiv V Gaikwad, Shambhudeo D Kharde

{"title":"CEA, CA 15-3, and miRNA expression as potential biomarkers in canine mammary tumors.","authors":"Mohit Jain, Shailesh D Ingole, Rahul S Deshmukh, Simin V Bharucha, Anagha S Nagvekar, Rajiv V Gaikwad, Shambhudeo D Kharde","doi":"10.1007/s10577-021-09652-7","DOIUrl":"https://doi.org/10.1007/s10577-021-09652-7","url":null,"abstract":"The most often detected tumor in intact bitches is mammary tumors and represents a significant clinical problem throughout the world. Mammary neoplasms in canine have heterogeneous morphology, so the choice of the most appropriate biomarker is the biggest challenge in CMT detection. We performed a retrospective analysis and evaluated the canine cancer antigens and miRNA expression profiles as potential biomarkers. Sixty dogs based on histological examination divided into three groups, viz., dogs with a benign mammary tumor, malignant mammary tumor, and control/healthy. The CA 15-3 was found more sensitive than CEA but detection of both will increase sensitivity. miR-21 expression differed significantly in all three groups. miR-29b expression differed significantly between the control and benign group and control and malignant group. The miR-21 overexpression and miR-29b downregulation with CMT are associated with clinical stage and can be used as non-invasive diagnostic and prognostic biomarkers. Hence, evaluation of CA 15-3 along with CEA would be a non-invasive technique for detecting canine mammary tumors. Evaluation of deregulated circulating miR-21 could be a valuable prognostic marker for early detection of mammary tumors in canines while miR-29b can add sensitivity in the detection of the canine mammary tumors if evaluated with miR-21.","PeriodicalId":50698,"journal":{"name":"Chromosome Research","volume":"29 2","pages":"175-188"},"PeriodicalIF":2.6,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-021-09652-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25411023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure. 先进的荧光纳米显微镜对揭示有丝分裂染色体结构的贡献。

IF 2.6 4区生物学

Chromosome Research Pub Date : 2021-03-01 Epub Date: 2021-03-09 DOI: 10.1007/s10577-021-09654-5

S W Botchway, S Farooq, A Sajid, I K Robinson, M Yusuf

{"title":"Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.","authors":"S W Botchway, S Farooq, A Sajid, I K Robinson, M Yusuf","doi":"10.1007/s10577-021-09654-5","DOIUrl":"https://doi.org/10.1007/s10577-021-09654-5","url":null,"abstract":"The organization of chromatin into higher-order structures and its condensation process represent one of the key challenges in structural biology. This is important for elucidating several disease states. To address this long-standing problem, development of advanced imaging methods has played an essential role in providing understanding into mitotic chromosome structure and compaction. Amongst these are two fast evolving fluorescence imaging technologies, specifically fluorescence lifetime imaging (FLIM) and super-resolution microscopy (SRM). FLIM in particular has been lacking in the application of chromosome research while SRM has been successfully applied although not widely. Both these techniques are capable of providing fluorescence imaging with nanometer information. SRM or \"nanoscopy\" is capable of generating images of DNA with less than 50 nm resolution while FLIM when coupled with energy transfer may provide less than 20 nm information. Here, we discuss the advantages and limitations of both methods followed by their contribution to mitotic chromosome studies. Furthermore, we highlight the future prospects of how advancements in new technologies can contribute in the field of chromosome science.","PeriodicalId":50698,"journal":{"name":"Chromosome Research","volume":"29 1","pages":"19-36"},"PeriodicalIF":2.6,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-021-09654-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25465799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Imaging the inner structure of chromosomes: contribution of focused ion beam/scanning electron microscopy to chromosome research. 染色体内部结构成像:聚焦离子束/扫描电镜对染色体研究的贡献。

IF 2.6 4区生物学

Chromosome Research Pub Date : 2021-03-01 Epub Date: 2021-02-15 DOI: 10.1007/s10577-021-09650-9

Astari Dwiranti, Fendi Sofyan Arifudin, Toshiyuki Wako, Kiichi Fukui

{"title":"Imaging the inner structure of chromosomes: contribution of focused ion beam/scanning electron microscopy to chromosome research.","authors":"Astari Dwiranti, Fendi Sofyan Arifudin, Toshiyuki Wako, Kiichi Fukui","doi":"10.1007/s10577-021-09650-9","DOIUrl":"https://doi.org/10.1007/s10577-021-09650-9","url":null,"abstract":"Visualization of the chromosome ultrastructure has revealed new insights into its structural and functional properties. The use of new methods for revealing not only the surface but also the inner structure of the chromosome has been emerged. Some methods have long been used, such as conventional transmission electron microscopy (TEM). Although it has indispensably contributed to the revelation of the ultrastructure of the various biological samples, including chromosomes, some challenges have also been encountered, such as laborious sample preparation, limited view areas, and loss of information on some parts due to ultramicrotome sectioning. Therefore, a more advanced method is needed. Scanning electron microscopy (SEM) is also advantageous in the surface visualization of chromosome samples. However, it is limited by accessibility to gain the inner structure information. Focused ion beam/scanning electron microscopy (FIB/SEM) provides a way to investigate the inner structure of the samples in a direct slice-and-view manner to observe the ultrastructure of the inner part of the sample continuously and further construct a three-dimensional image. This method has long been used in the material science field, and recently, it has also been applied to biological research, such as in showing the inner structure of chromosomes. This review article presents the contributions of this new method to chromosome research and its recent developments in the inner structure of chromosome and discusses its current and potential applications to the high-resolution imaging of chromosomes.","PeriodicalId":50698,"journal":{"name":"Chromosome Research","volume":"29 1","pages":"51-62"},"PeriodicalIF":2.6,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-021-09650-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25369424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imaging approaches for chromosome structures. 染色体结构的成像方法。

IF 2.6 4区生物学

Chromosome Research Pub Date : 2021-03-01 Epub Date: 2021-02-15 DOI: 10.1007/s10577-021-09648-3

Kiichi Fukui, Seiji Kato

{"title":"Imaging approaches for chromosome structures.","authors":"Kiichi Fukui, Seiji Kato","doi":"10.1007/s10577-021-09648-3","DOIUrl":"https://doi.org/10.1007/s10577-021-09648-3","url":null,"abstract":"This review describes image analyses for chromosome visible structures, focusing on the chromosome imaging system CHIAS (Chromosome Image Analyzing System). CHIAS is the first comprehensive imaging system for the analysis and characterization of plant chromosomes. A simulation method for human vision for capturing band positive regions was developed and used for the image analysis of large plant chromosomes with bands. Applying this method to C-banded Crepis chromosomes enabled recognition of band positive regions as seen by human vision. Furthermore, a new image parameter, condensation pattern was developed and successfully applied to identify small plant chromosomes such as rice and brassicas. Condensation profile (CP) derived from condensation pattern was also effective in developing quantitative chromosome maps. The result was quantitative chromosomal maps of several plants with small chromosomes, including Arabidopsis, diploid brassicas, rapeseed, rice, spinach, and sugarcane. In the final chapter, various applications of imaging techniques to the analysis of pachytene chromosomes, improved visibility of multicolor FISH images, 3D reconstruction of a human chromosome based on cross-section images obtained by a FIB/SEM, automatic extraction of chromosomal regions by machine learning, etc. are described.","PeriodicalId":50698,"journal":{"name":"Chromosome Research","volume":"29 1","pages":"5-17"},"PeriodicalIF":2.6,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-021-09648-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25369423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seeing chromosome structure reveals its function. 观察染色体结构就能发现它的功能。

IF 2.6 4区生物学

Chromosome Research Pub Date : 2021-03-01 DOI: 10.1007/s10577-021-09657-2

Kiichi Fukui, Toshiyuki Wako

引用次数: 0

X-ray Ptychography Imaging of Human Chromosomes After Low-dose Irradiation. 低剂量辐照后人类染色体的 X 射线层析成像。

IF 2.4 4区生物学

Chromosome Research Pub Date : 2021-03-01 Epub Date: 2021-03-31 DOI: 10.1007/s10577-021-09660-7

Archana Bhartiya, Darren Batey, Silvia Cipiccia, Xiaowen Shi, Christoph Rau, Stanley Botchway, Mohammed Yusuf, Ian K Robinson

引用次数: 0