厚柱头菟丝子亚属核型不对称反映了其重复DNA组成。

IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Chromosome Research Pub Date : 2022-03-01 Epub Date: 2022-01-28 DOI:10.1007/s10577-021-09683-0
Amalia Ibiapino, Mariana Báez, Miguel A García, Mihai Costea, Saša Stefanović, Andrea Pedrosa-Harand
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引用次数: 5

摘要

菟丝子是一个细胞遗传学多样化的属,其核型在染色体数目上变化18倍,在基因组大小上变化127倍。其四个亚属中的每一个亚属也表现出特定的染色体特征,例如厚柱头的双峰核型。为了了解双峰核型的起源,我们对粗柱头亚属(Cuscuta nitida)的基因组进行了低覆盖率测序,并对三个亚属代表进行了染色体显带和分子细胞遗传学分析。nitida、C. africana (2n = 28)和C. angulata (2n = 30)在最大的染色体对上均显示异色带。在黑螺旋藻基因组中鉴定出18个卫星dna,其中2个与移动元件相似。最丰富的存在于最大的对,以及高度丰富的核糖体dna。最丰富的Ty1/Copia和Ty3/Gypsy元素也高度富集在最大的对上,除了Ty3/Gypsy CRM,它也标记了最小染色体的中心点周围区域。这种大对重复DNA的积累表明,这些序列在很大程度上负责在肿柱头亚属的双峰核型的形成。重复DNA片段与库斯库塔的核型进化直接相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Karyotype asymmetry in Cuscuta L. subgenus Pachystigma reflects its repeat DNA composition.

Cuscuta is a cytogenetically diverse genus, with karyotypes varying 18-fold in chromosome number and 127-fold in genome size. Each of its four subgenera also presents particular chromosomal features, such as bimodal karyotypes in Pachystigma. We used low coverage sequencing of the Cuscuta nitida genome (subgenus Pachystigma), as well as chromosome banding and molecular cytogenetics of three subgenus representatives, to understand the origin of bimodal karyotypes. All three species, C. nitida, C. africana (2n = 28) and C. angulata (2n = 30), showed heterochromatic bands mainly in the largest chromosome pairs. Eighteen satellite DNAs were identified in C. nitida genome, two showing similarity to mobile elements. The most abundant were present at the largest pairs, as well as the highly abundant ribosomal DNAs. The most abundant Ty1/Copia and Ty3/Gypsy elements were also highly enriched in the largest pairs, except for the Ty3/Gypsy CRM, which also labelled the pericentromeric regions of the smallest chromosomes. This accumulation of repetitive DNA in the larger pairs indicates that these sequences are largely responsible for the formation of bimodal karyotypes in the subgenus Pachystigma. The repetitive DNA fraction is directly linked to karyotype evolution in Cuscuta.

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来源期刊
Chromosome Research
Chromosome Research 生物-生化与分子生物学
CiteScore
4.70
自引率
3.80%
发文量
31
审稿时长
1 months
期刊介绍: Chromosome Research publishes manuscripts from work based on all organisms and encourages submissions in the following areas including, but not limited, to: · Chromosomes and their linkage to diseases; · Chromosome organization within the nucleus; · Chromatin biology (transcription, non-coding RNA, etc); · Chromosome structure, function and mechanics; · Chromosome and DNA repair; · Epigenetic chromosomal functions (centromeres, telomeres, replication, imprinting, dosage compensation, sex determination, chromosome remodeling); · Architectural/epigenomic organization of the genome; · Functional annotation of the genome; · Functional and comparative genomics in plants and animals; · Karyology studies that help resolve difficult taxonomic problems or that provide clues to fundamental mechanisms of genome and karyotype evolution in plants and animals; · Mitosis and Meiosis; · Cancer cytogenomics.
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