Czech Journal of Genetics and Plant Breeding最新文献_第6页

Mendelian inheritance of introrse orientated anthers in Brassica rapa 油菜花药内向取向的孟德尔遗传

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-03-04 DOI: 10.17221/107/2021-cjgpb

J. Salava, D. Lydiate

引用次数: 0

Screening and validation of three molecular markers for disease resistance in eggplant 茄子抗病性三个分子标记的筛选与鉴定

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-02-17 DOI: 10.17221/105/2021-cjgpb

G. Sülü, İ. Polat, H. Boyaci, A. Yildirim, E. Gümrükcü

{"title":"Screening and validation of three molecular markers for disease resistance in eggplant","authors":"G. Sülü, İ. Polat, H. Boyaci, A. Yildirim, E. Gümrükcü","doi":"10.17221/105/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/105/2021-cjgpb","url":null,"abstract":"Fusarium oxysporum Schlecht. f.sp. melongenae (FOM), Verticillium dahliae (Ve) and Ralstonia solanacearum are major limiting pathogens affecting eggplant cultivation and their yield in the world. Natural resistance genes are the most environmentally friendly method to control the disease. Thus, marker-assisted selection (MAS) is preferred as a tool for screening resistance genes (R-genes) in eggplant resistance breeding. In this study, markers that are specifically linked to major disease resistance genes conferring resistance to F. melongenae, V. dahliae and R. solanacearum were tested in a population containing breeding materials to validate the resistance. Resistant Solanum melongena accessions LS1934 and LS2436 and their reciprocal crosses were used as the resistance resource for this validation study. Moreover, classical resistance tests to FOM and Ve were performed with the root-dip inoculation method for classification of all the accessions based on their resistance/susceptibility responses. The SCAR426, CAPs_903 and SIVR844 markers were highly informative for the determination of resistance genes (Fomg, Ve and ERs1). Therefore, in areas with high susceptibility to diseases, a highly efficient combination of the relevant R-genes and their pyramiding into commercial eggplant varieties are proposed to be implemented as a pragmatic approach.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45411162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on fine mapping of QTL for juice yield traits of sweet sorghum (Sorghum dochna) 甜高粱产汁性状QTL精细定位研究

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-01-05 DOI: 10.17221/48/2021-cjgpb

Bo Zhao, Buxian Xia, Jianming Gao, Feng Luo, Qiu-Xing Chen, J. Lv, Ou-Jing Li, Jingwang Li, Xiaopeng Tong, Hui-min Liu, Shoujun Sun, Z. Pei

{"title":"Study on fine mapping of QTL for juice yield traits of sweet sorghum (Sorghum dochna)","authors":"Bo Zhao, Buxian Xia, Jianming Gao, Feng Luo, Qiu-Xing Chen, J. Lv, Ou-Jing Li, Jingwang Li, Xiaopeng Tong, Hui-min Liu, Shoujun Sun, Z. Pei","doi":"10.17221/48/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/48/2021-cjgpb","url":null,"abstract":"The stem juice yield is a key factor that influences both the biological and economic production of sweet sorghum [Sorghum dochna (Forssk.) Snowden]. To elucidate upon the genetic basis of the stem juice yield, an F5 population developed from a cross between the low juice yielding Xinliang52 (XL52) and high juice yielding W455 lines, were used in a quantitative trait locus (QTL) analysis. A main effect of the QTL controlling stem juice yield was separated with an SSR marker called Xtxp97, which explained 46.7% of the phenotypic variance. In addition, F5 and F6 populations were constructed with XL52 and W452 as the parents to further verify the QTLs, and a significant correlation was found between the juice yield trait and the Xtxp97 marker. Based on the progeny tests of 29 recombinants, QJy-sbi06 was located in a region of about 21.2 kb on chromosome 6, where a candidate gene encoding an NAC transcription factor (sobic.006G147400) was identified. Combining the different population association analysis and sequencing technology showed that XL52 inserted a 1.8 kb transposon in the NAC to directly interrupt and inactivate the juice yield gene. This study also demonstrated that the colour of the leaf midribs was controlled by a single gene and was significantly positive correlated with juiciness (r = 0.784, P < 0.01). These results could lay the foundation for map-based cloning of QJy-sbi06 and provide genes or QTLs for breeding sorghum lines with a high juice yield and quality.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2022-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47974901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Breeding for salt tolerance in wheat: The contribution of carbon isotopic signatures 小麦耐盐育种:碳同位素特征的贡献

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-01-05 DOI: 10.17221/51/2021-cjgpb

Chamekh Zoubeir, Inès Zouari, Salma Jallouli, S. Ayadi, Sebei Abdennour, Y. Trifa

引用次数: 1

Index of Volume 57 (2021) - Authors Index 第57卷索引（2021）-作者索引

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-12-31 DOI: 10.17221/13764-cjgpb

引用次数: 0

Evaluation of resistance to Pseudoperonospora humuli and of the content of alpha acids and hop oils in hops of selected genetic resources of hop Humulus lupulus L. 葎草遗传资源对腐殖质假假性孢子虫的抗性及酒花中α酸和酒花油含量的测定。

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-12-09 DOI: 10.17221/70/2021-cjgpb

Markéta Trefilová, V. Nesvadba, J. Charvátová

引用次数: 3

Morpho-molecular diversity study of rice cultivars in Bangladesh 孟加拉国水稻品种形态-分子多样性研究

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-12-06 DOI: 10.17221/69/2021-cjgpb

Md. Babul Akter, Azad Mosab-Bin, M. Kamruzzaman, R. Reflinur, N. Nahar, M. Rana, M. Hoque, M. Islam

{"title":"Morpho-molecular diversity study of rice cultivars in Bangladesh","authors":"Md. Babul Akter, Azad Mosab-Bin, M. Kamruzzaman, R. Reflinur, N. Nahar, M. Rana, M. Hoque, M. Islam","doi":"10.17221/69/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/69/2021-cjgpb","url":null,"abstract":"Rice is one of the frontline cereals in the world and the major cultivated crop in Bangladesh. A total of eleven simple sequence repeats (SSRs) and thirteen sequence-tagged site (STS) markers were used to characterize twenty-four rice cultivars in Bangladesh. Twenty-four markers generated 60 alleles with 2.5 alleles per locus. The average polymorphism information content (PIC) value was 0.40, while the mean value of heterozygosity, gene diversity, and major allele frequency were recorded as 0.10, 0.48 and 0.62, respectively. However, the SSR markers showed more specificity and a higher discrimination power than the STS markers. The cluster analysis displayed four major clusters with a genetic similarity coefficient value of 0.73. The morphological analyses of the grain identified that Binadhan-20 and BRRI dhan34 had the longest and the shortest seed size, respectively, with a variable correlation between the seed length, width and length/width ratio. The phenol reaction test distinguished seven cultivars as japonica and seventeen cultivars as indica or an intermediate type. All these results regarding the phenotypic data and marker information will be useful for parental selection in modern rice breeding programmes.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44110077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Disease resistance of improved MR220 lines against Pyricularia oryzae Cavara and their preliminary agronomic performance MR220改良系对稻瘟病菌的抗病性及其初步农艺性能

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-11-24 DOI: 10.17221/55/2021-cjgpb

Siti Nor Hafiza Mohamad, Mohamad Bahagia Ab Ghaffar, A. S. Othman, S. N. Misman, Zuraida Abd Rahman, Mohd Shahril Firdaus Ab Razak, Z. A. Seman, Muhammad Fairuz Mohd Yusof, Khairulmazmi Ahmad, S. N. Yazid, H. Hashim

{"title":"Disease resistance of improved MR220 lines against Pyricularia oryzae Cavara and their preliminary agronomic performance","authors":"Siti Nor Hafiza Mohamad, Mohamad Bahagia Ab Ghaffar, A. S. Othman, S. N. Misman, Zuraida Abd Rahman, Mohd Shahril Firdaus Ab Razak, Z. A. Seman, Muhammad Fairuz Mohd Yusof, Khairulmazmi Ahmad, S. N. Yazid, H. Hashim","doi":"10.17221/55/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/55/2021-cjgpb","url":null,"abstract":"Blast disease caused by Pyricularia oryzae is one of the most destructive fungal diseases of rice in Malaysia. Utilisation of resistant varieties is the most efficient management approach towards reducing yield losses. The line IRTP21683 with the Pi9 gene has shown strong resistance against the isolate MPO988.3 of pathotype P0.0, the most prevalent P. oryzae pathotype in Malaysia. Crossing of IRTP21683 was undertaken with the recurrent parent MR220, a susceptible elite Malaysian rice variety, using a marker assisted backcrossing technique with two simple sequence repeat markers, RM19776 and RM7311, as the tag for the Pi9 gene. Twenty BC3F4 lines with the Pi9 gene were resistant when challenged with MPO 988.3. The cluster analysis based on seven agronomic parameters showed that the resistant BC3F4 lines could be divided into four groups, of which the members in group 1 and 2 have shown comparable or better performance than MR220. Five lines in group 1, B220PI9-3-48, B220PI9-3-76, B220PI9-3-77, B220PI9-3-79 and B220PI9-3-82 showed outstanding yield performance with early maturation.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48023989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of late blight resistance R genes and their coding proteins in some potato genotypes 马铃薯抗晚疫病R基因及其编码蛋白的比较分析

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-11-04 DOI: 10.17221/49/2021-cjgpb

H. Mahfouze, O. E. El-Sayed

{"title":"Comparative analysis of late blight resistance R genes and their coding proteins in some potato genotypes","authors":"H. Mahfouze, O. E. El-Sayed","doi":"10.17221/49/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/49/2021-cjgpb","url":null,"abstract":"Late blight (LB) disease can cause potato yield losses in both Egypt and the world. Therefore, the structural analysis of resistance (R) genes responsible for LB resistance will help in understanding their functions. This work aimed to identify the variations between the dominant and recessive alleles of two genes, R3a and R8 at the nucleotide and amino acid levels in five potato genotypes. Two genes of R3a and R8 representing the broad-spectrum LB resistance were amplified by specific primers, which gave one amplicon of 194 and 220 bp of each gene, respectively. Two fragments were sequenced after purification using an ABI 3730xl System DNA Sequencer. The DNA sequence alignments of two genes, R3a and R8, were determined among five selected potato genotypes. The percentage of genetic similarity of the nucleotide sequences of the R3a and R8 genes ranged between (82–83%) and (86–87%), respectively, in comparison to the reference sequences in the nucleotide BLAST. We report on the existence of positional differences in the nucleotide sequences, and base-pair substitutions of two fragments, resulting in amino acid changes between the resistant and susceptible potato genotypes. On the other hand, the highest total number of base-pair substitutions was recorded as 16 in the recessive allele r8 of the varieties Bellini and Cara. However, the lowest number was recorded as four in the dominant allele R3a of the variety Cara. The dendrograms of the five potato genotypes were made up of phylogenetically different clusters, separate from all the other named potato accessions of the two genes. The results of this study will create a solid base for the further understanding of the mechanism of plant-pathogen interactions and supply a theoretical reference for durable resistance to late blight diseases in the potato.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49025248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A 31-bp indel localised in the 5' untranslated region of OsSUT3 affects the gene expression and rice (Oryza sativa L.) pollen development 位于OsSUT3基因5'非翻译区一个31 bp的缺失影响基因表达和水稻花粉发育

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-11-01 DOI: 10.17221/66/2021-cjgpb

Chunlong Zhang, Qiuping Li, Hong Yang, Tuo Wang, Juan Li, J. Wen, S. Jin, Zhonglin Zhang, Lijuan Chen, Dandan Li

引用次数: 2