Czech Journal of Genetics and Plant Breeding最新文献_第5页

The sweet potato transcription factor IbbHLH33 enhances chilling tolerance in transgenic tobacco 甘薯转录因子IbbHLH33增强转基因烟草抗寒性

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-04-06 DOI: 10.17221/115/2021-cjgpb

Tao Yu, Huanan Zhou, Z. Liu, H. Zhai, Qingchang Liu

{"title":"The sweet potato transcription factor IbbHLH33 enhances chilling tolerance in transgenic tobacco","authors":"Tao Yu, Huanan Zhou, Z. Liu, H. Zhai, Qingchang Liu","doi":"10.17221/115/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/115/2021-cjgpb","url":null,"abstract":"Chilling is an important abiotic stress in plants. Sweet potato is sensitive to cold damage due to its tropical origin. In this study, we identified a basic helix-loop-helix (bHLH) gene, IbbHLH33, from our cold-tolerance-related transcriptomic data. Further analyses revealed that IbbHLH33 encoded a nuclear protein and was most closely related to AtbHLH33. RT-qPCR analysis showed that IbbHLH33 was expressed at the highest level in the roots, and its expression was strongly induced by low temperature (4 °C), H2O2 and abscisic acid (ABA) treatments. Transgenic tobacco plants overexpressing IbbHLH33 were obtained by Agrobacterium-mediated transformation, which enhanced the chilling resistance of tobacco. At low temperatures, the proline content, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content increased significantly, while the relative conductivity decreased significantly. At the same time, the expression of proline synthesis related genes and antioxidant activity related genes increased, while the expression of ABA synthesis related genes decreased. The results showed that IbbHLH33 is a transcription factor encoding a gene of the bHLH family that regulates chilling tolerance. In conclusion, these data suggest that IbbHLH33 has the potential to improve chilling tolerance in tobacco and other plants.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43643196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Chromosome-centric approaches in crop genomics: Focus on Mendel’s pea plant 以染色体为中心的作物基因组学方法:以孟德尔的豌豆植物为重点

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-04-04 DOI: 10.17221/11/2022-cjgpb

Petr Cápal, J. Doležel

引用次数: 0

Validation of CAPS marker WR003 for the leaf rust resistance gene Lr1 and the molecular evolution of Lr1 in wheat 小麦抗叶锈病基因Lr1的CAPS标记WR003验证及Lr1的分子进化

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-03-04 DOI: 10.17221/119/2021-cjgpb

Xian-jun Liu, Xinchun Liu, Hongyan Sun, Chunyan Hao, Xiaoxiao Wang, Zhijiang Rong, Zongyun Feng

{"title":"Validation of CAPS marker WR003 for the leaf rust resistance gene Lr1 and the molecular evolution of Lr1 in wheat","authors":"Xian-jun Liu, Xinchun Liu, Hongyan Sun, Chunyan Hao, Xiaoxiao Wang, Zhijiang Rong, Zongyun Feng","doi":"10.17221/119/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/119/2021-cjgpb","url":null,"abstract":"The wheat leaf rust resistance gene Lr1 encodes a typical coiled-coil nucleotide-binding site leucine-rich repeat (CC-NBS-LRR) of resistance protein containing 1 344 amino acids. WR003, a cleaved amplified polymorphic sequence (CAPS) marker is derived from the LRR regions of Lr1. In this study, a worldwide collection of 120 Aegilops tauschii accessions and 282 hexaploid wheat varieties was screened for Lr1 alleles using WR003, and the specificity of WR003 for Lr1 was confirmed by pathogenicity tests and genotype analysis. The sequence alignment and phylogenetic tree analysis of 38 Lr1 haplotypes provided a further view of the molecular evolution of Lr1. The results showed that there were very few polymorphisms between the Lr1 alleles from Ae. tauschii and hexaploid wheat with the same resistance phenotype. The polymorphisms of the Lr1 haplotypes were mainly between the different resistance lines, rather than between the different ploidy levels. These results indicate that Lr1 originated from Ae. tauschii and differentiated into resistant and susceptible genotypes before its introgression into hexaploid wheat. Therefore, it is likely that wheat Lr1 has at least two major variants for disease resistance and susceptibility, and except for certain point mutations, few gene conversions and genetic re-combinations occurred during the hexaploid wheat domestication.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47224683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Mendelian inheritance of introrse orientated anthers in Brassica rapa 油菜花药内向取向的孟德尔遗传

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-03-04 DOI: 10.17221/107/2021-cjgpb

J. Salava, D. Lydiate

引用次数: 0

Screening and validation of three molecular markers for disease resistance in eggplant 茄子抗病性三个分子标记的筛选与鉴定

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-02-17 DOI: 10.17221/105/2021-cjgpb

G. Sülü, İ. Polat, H. Boyaci, A. Yildirim, E. Gümrükcü

{"title":"Screening and validation of three molecular markers for disease resistance in eggplant","authors":"G. Sülü, İ. Polat, H. Boyaci, A. Yildirim, E. Gümrükcü","doi":"10.17221/105/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/105/2021-cjgpb","url":null,"abstract":"Fusarium oxysporum Schlecht. f.sp. melongenae (FOM), Verticillium dahliae (Ve) and Ralstonia solanacearum are major limiting pathogens affecting eggplant cultivation and their yield in the world. Natural resistance genes are the most environmentally friendly method to control the disease. Thus, marker-assisted selection (MAS) is preferred as a tool for screening resistance genes (R-genes) in eggplant resistance breeding. In this study, markers that are specifically linked to major disease resistance genes conferring resistance to F. melongenae, V. dahliae and R. solanacearum were tested in a population containing breeding materials to validate the resistance. Resistant Solanum melongena accessions LS1934 and LS2436 and their reciprocal crosses were used as the resistance resource for this validation study. Moreover, classical resistance tests to FOM and Ve were performed with the root-dip inoculation method for classification of all the accessions based on their resistance/susceptibility responses. The SCAR426, CAPs_903 and SIVR844 markers were highly informative for the determination of resistance genes (Fomg, Ve and ERs1). Therefore, in areas with high susceptibility to diseases, a highly efficient combination of the relevant R-genes and their pyramiding into commercial eggplant varieties are proposed to be implemented as a pragmatic approach.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45411162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study on fine mapping of QTL for juice yield traits of sweet sorghum (Sorghum dochna) 甜高粱产汁性状QTL精细定位研究

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-01-05 DOI: 10.17221/48/2021-cjgpb

Bo Zhao, Buxian Xia, Jianming Gao, Feng Luo, Qiu-Xing Chen, J. Lv, Ou-Jing Li, Jingwang Li, Xiaopeng Tong, Hui-min Liu, Shoujun Sun, Z. Pei

{"title":"Study on fine mapping of QTL for juice yield traits of sweet sorghum (Sorghum dochna)","authors":"Bo Zhao, Buxian Xia, Jianming Gao, Feng Luo, Qiu-Xing Chen, J. Lv, Ou-Jing Li, Jingwang Li, Xiaopeng Tong, Hui-min Liu, Shoujun Sun, Z. Pei","doi":"10.17221/48/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/48/2021-cjgpb","url":null,"abstract":"The stem juice yield is a key factor that influences both the biological and economic production of sweet sorghum [Sorghum dochna (Forssk.) Snowden]. To elucidate upon the genetic basis of the stem juice yield, an F5 population developed from a cross between the low juice yielding Xinliang52 (XL52) and high juice yielding W455 lines, were used in a quantitative trait locus (QTL) analysis. A main effect of the QTL controlling stem juice yield was separated with an SSR marker called Xtxp97, which explained 46.7% of the phenotypic variance. In addition, F5 and F6 populations were constructed with XL52 and W452 as the parents to further verify the QTLs, and a significant correlation was found between the juice yield trait and the Xtxp97 marker. Based on the progeny tests of 29 recombinants, QJy-sbi06 was located in a region of about 21.2 kb on chromosome 6, where a candidate gene encoding an NAC transcription factor (sobic.006G147400) was identified. Combining the different population association analysis and sequencing technology showed that XL52 inserted a 1.8 kb transposon in the NAC to directly interrupt and inactivate the juice yield gene. This study also demonstrated that the colour of the leaf midribs was controlled by a single gene and was significantly positive correlated with juiciness (r = 0.784, P < 0.01). These results could lay the foundation for map-based cloning of QJy-sbi06 and provide genes or QTLs for breeding sorghum lines with a high juice yield and quality.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2022-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47974901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Breeding for salt tolerance in wheat: The contribution of carbon isotopic signatures 小麦耐盐育种:碳同位素特征的贡献

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-01-05 DOI: 10.17221/51/2021-cjgpb

Chamekh Zoubeir, Inès Zouari, Salma Jallouli, S. Ayadi, Sebei Abdennour, Y. Trifa

引用次数: 1

Index of Volume 57 (2021) - Authors Index 第57卷索引（2021）-作者索引

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-12-31 DOI: 10.17221/13764-cjgpb

引用次数: 0

Evaluation of resistance to Pseudoperonospora humuli and of the content of alpha acids and hop oils in hops of selected genetic resources of hop Humulus lupulus L. 葎草遗传资源对腐殖质假假性孢子虫的抗性及酒花中α酸和酒花油含量的测定。

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-12-09 DOI: 10.17221/70/2021-cjgpb

Markéta Trefilová, V. Nesvadba, J. Charvátová

引用次数: 3

Morpho-molecular diversity study of rice cultivars in Bangladesh 孟加拉国水稻品种形态-分子多样性研究

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-12-06 DOI: 10.17221/69/2021-cjgpb

Md. Babul Akter, Azad Mosab-Bin, M. Kamruzzaman, R. Reflinur, N. Nahar, M. Rana, M. Hoque, M. Islam

{"title":"Morpho-molecular diversity study of rice cultivars in Bangladesh","authors":"Md. Babul Akter, Azad Mosab-Bin, M. Kamruzzaman, R. Reflinur, N. Nahar, M. Rana, M. Hoque, M. Islam","doi":"10.17221/69/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/69/2021-cjgpb","url":null,"abstract":"Rice is one of the frontline cereals in the world and the major cultivated crop in Bangladesh. A total of eleven simple sequence repeats (SSRs) and thirteen sequence-tagged site (STS) markers were used to characterize twenty-four rice cultivars in Bangladesh. Twenty-four markers generated 60 alleles with 2.5 alleles per locus. The average polymorphism information content (PIC) value was 0.40, while the mean value of heterozygosity, gene diversity, and major allele frequency were recorded as 0.10, 0.48 and 0.62, respectively. However, the SSR markers showed more specificity and a higher discrimination power than the STS markers. The cluster analysis displayed four major clusters with a genetic similarity coefficient value of 0.73. The morphological analyses of the grain identified that Binadhan-20 and BRRI dhan34 had the longest and the shortest seed size, respectively, with a variable correlation between the seed length, width and length/width ratio. The phenol reaction test distinguished seven cultivars as japonica and seventeen cultivars as indica or an intermediate type. All these results regarding the phenotypic data and marker information will be useful for parental selection in modern rice breeding programmes.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44110077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3