Study on fine mapping of QTL for juice yield traits of sweet sorghum (Sorghum dochna)

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-01-05 DOI:10.17221/48/2021-cjgpb

Bo Zhao, Buxian Xia, Jianming Gao, Feng Luo, Qiu-Xing Chen, J. Lv, Ou-Jing Li, Jingwang Li, Xiaopeng Tong, Hui-min Liu, Shoujun Sun, Z. Pei

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引用次数: 1

Abstract

The stem juice yield is a key factor that influences both the biological and economic production of sweet sorghum [Sorghum dochna (Forssk.) Snowden]. To elucidate upon the genetic basis of the stem juice yield, an F5 population developed from a cross between the low juice yielding Xinliang52 (XL52) and high juice yielding W455 lines, were used in a quantitative trait locus (QTL) analysis. A main effect of the QTL controlling stem juice yield was separated with an SSR marker called Xtxp97, which explained 46.7% of the phenotypic variance. In addition, F5 and F6 populations were constructed with XL52 and W452 as the parents to further verify the QTLs, and a significant correlation was found between the juice yield trait and the Xtxp97 marker. Based on the progeny tests of 29 recombinants, QJy-sbi06 was located in a region of about 21.2 kb on chromosome 6, where a candidate gene encoding an NAC transcription factor (sobic.006G147400) was identified. Combining the different population association analysis and sequencing technology showed that XL52 inserted a 1.8 kb transposon in the NAC to directly interrupt and inactivate the juice yield gene. This study also demonstrated that the colour of the leaf midribs was controlled by a single gene and was significantly positive correlated with juiciness (r = 0.784, P < 0.01). These results could lay the foundation for map-based cloning of QJy-sbi06 and provide genes or QTLs for breeding sorghum lines with a high juice yield and quality.

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甜高粱产汁性状QTL精细定位研究

茎汁产量是影响甜高粱生物生产和经济生产的关键因素。斯诺登)。为阐明茎汁产量的遗传基础，以低汁系新良52 (XL52)与高产系W455杂交而成的F5群体为材料，进行了数量性状位点(QTL)分析。用SSR标记Xtxp97分离出控制茎汁产量的QTL主效应，解释了46.7%的表型变异。此外，以XL52和W452为亲本构建F5和F6群体进一步验证qtl，发现产汁性状与Xtxp97标记之间存在显著相关。通过对29个重组体的子代检测，QJy-sbi06位于6号染色体约21.2 kb的区域，在该区域发现了一个编码NAC转录因子的候选基因(sobic.006G147400)。结合不同群体关联分析和测序技术发现，XL52在NAC中插入了一个1.8 kb的转座子，直接中断并灭活了产汁基因。叶中肋颜色受单基因控制，与多汁性呈极显著正相关(r = 0.784, P < 0.01)。这些结果可为QJy-sbi06的图谱克隆奠定基础，并为选育高产优质高粱品系提供基因或qtl。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.