The sweet potato transcription factor IbbHLH33 enhances chilling tolerance in transgenic tobacco

IF 1.2 4区农林科学 Q3 AGRONOMY

Czech Journal of Genetics and Plant Breeding Pub Date : 2022-04-06 DOI:10.17221/115/2021-cjgpb

Tao Yu, Huanan Zhou, Z. Liu, H. Zhai, Qingchang Liu

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引用次数: 1

Abstract

Chilling is an important abiotic stress in plants. Sweet potato is sensitive to cold damage due to its tropical origin. In this study, we identified a basic helix-loop-helix (bHLH) gene, IbbHLH33, from our cold-tolerance-related transcriptomic data. Further analyses revealed that IbbHLH33 encoded a nuclear protein and was most closely related to AtbHLH33. RT-qPCR analysis showed that IbbHLH33 was expressed at the highest level in the roots, and its expression was strongly induced by low temperature (4 °C), H2O2 and abscisic acid (ABA) treatments. Transgenic tobacco plants overexpressing IbbHLH33 were obtained by Agrobacterium-mediated transformation, which enhanced the chilling resistance of tobacco. At low temperatures, the proline content, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content increased significantly, while the relative conductivity decreased significantly. At the same time, the expression of proline synthesis related genes and antioxidant activity related genes increased, while the expression of ABA synthesis related genes decreased. The results showed that IbbHLH33 is a transcription factor encoding a gene of the bHLH family that regulates chilling tolerance. In conclusion, these data suggest that IbbHLH33 has the potential to improve chilling tolerance in tobacco and other plants.

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甘薯转录因子IbbHLH33增强转基因烟草抗寒性

低温胁迫是植物中一种重要的非生物胁迫。由于甘薯的原产地是热带，所以对冷害很敏感。在这项研究中，我们从我们的耐寒性相关转录组数据中鉴定出一个基本的螺旋-环-螺旋(bHLH)基因IbbHLH33。进一步分析发现，IbbHLH33编码一个核蛋白，与AtbHLH33亲缘关系最为密切。RT-qPCR分析显示，IbbHLH33在根中表达量最高，低温(4℃)、H2O2和ABA处理对IbbHLH33的表达有强烈的诱导作用。通过农杆菌介导转化获得了过表达IbbHLH33的转基因烟草植株，提高了烟草的抗寒性。低温处理下，脯氨酸含量、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量显著升高，相对电导率显著降低。同时脯氨酸合成相关基因和抗氧化活性相关基因表达量增加，ABA合成相关基因表达量减少。结果表明，IbbHLH33是bHLH家族中调控低温耐受性基因的转录因子。综上所述，这些数据表明IbbHLH33具有提高烟草和其他植物耐冷性的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Czech Journal of Genetics and Plant Breeding Agricultural and Biological Sciences-Plant Science

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Original scientific papers, critical reviews articles and short communications from the field of theoretical and applied plant genetics, plant biotechnology and plant breeding. Papers are published in English.