Czech Journal of Genetics and Plant Breeding最新文献_第10页

Comprehensive genomic analysis and expression profiling of the BTB and TAZ (BT) genes in cucumber (Cucumis sativus L.) 黄瓜BTB和TAZ（BT）基因的综合基因组分析及表达谱

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-12-09 DOI: 10.17221/34/2019-cjgpb

Yong Zhou, Guanghua Li, Lin Zhang, Jie Xu, Lifang Hu, Lunwei Jiang, Shiqiang Liu

引用次数: 6

Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCR 冬小麦品种对眼斑病反应的目测和实时PCR分析

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-12-09 DOI: 10.17221/52/2019-cjgpb

J. Palicová, P. Matušinský, A. Hanzalová, I. Svačinová, V. Dumalasová, J. Chrpová

{"title":"Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCR","authors":"J. Palicová, P. Matušinský, A. Hanzalová, I. Svačinová, V. Dumalasová, J. Chrpová","doi":"10.17221/52/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/52/2019-cjgpb","url":null,"abstract":"The reaction of twelve winter wheat cultivars frequently grown in the Czech Republic and twenty-five new breeding lines to inoculation with Oculimacula yallundae and Oculimacula acuformis was evaluated in small plot trials from 2017–2018. The assessment was carried out visually by symptoms and by a quantitative real-time polymerase chain reaction (qPCR). The aims of the study were to compare the results of both methods, to evaluate the effect of the resistance gene Pch1 to eyespot, and to select new breeding lines resistant to eyespot. The relationship between the eyespot symptoms and the pathogen DNA content in plant tissues followed a moderate linear regression. Low levels of eyespot were observed in the cultivars/lines possessing the resistance gene Pch1 (Annie, Hermann, Rebell, SG-S1215-14, SG-S1825-14, SG-S791-13) and also in the line SG-SU630-15. The qPCR method was able to detect low levels of the pathogens in the plant tissue and to distinguish two eyespot pathogens. O. acuformis was detected in very low concentrations in the inoculated plants compared with O. yallundae. The eyespot infection rate was significantly higher in 2017 than in the next agricultural season due to extremely dry and warm spring weather in 2018.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"56 1","pages":"9-14"},"PeriodicalIF":0.9,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/52/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43309974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Comparative metabolomics and transcriptomics of pistils, stamens and pistilloid stamens widen key knowledge of pistil and stamen development in wheat. 雌蕊、雄蕊和似雌蕊的比较代谢组学和转录组学拓宽了小麦雌蕊发育的关键知识。

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-12-09 DOI: 10.17221/70/2019-cjgpb

Yan Yu, Zhengsong Peng, Jipeng Qu, Z. Chen, Shuhong Wei, M. Liao, Li Zhang, Zaijun Yang

{"title":"Comparative metabolomics and transcriptomics of pistils, stamens and pistilloid stamens widen key knowledge of pistil and stamen development in wheat.","authors":"Yan Yu, Zhengsong Peng, Jipeng Qu, Z. Chen, Shuhong Wei, M. Liao, Li Zhang, Zaijun Yang","doi":"10.17221/70/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/70/2019-cjgpb","url":null,"abstract":"To examine the role of metabolites in wheat stamen and pistil development, metabolomic analyses of pistilloid stamens (PS), pistils (P), and stamens (S) from a novel wheat mutant homologous transformation sterility-1 (HTS-1) and controls from their sib-line CSTP were conducted using base gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). Then, the metabolomic data were integrated with previously published transcriptomic data and analysed. In total, 141 annotated metabolites were determined from P, PS and S tissues by comparison with reference standards. A total of 90, 93 and 18 different metabolites were identified in S vs. PS, S vs. P and P vs. PS, respectively. Among the different metabolites, 80 may be associated with stamen and pistil growth. Using integration evaluations of both the previous transcriptome data and the 80 various metabolites, we found two perturbed pathways that significantly affect flower development in plants, namely, the phenylpropanoid biosynthesis and cysteine and methionine metabolism. The ethylene synthesis pathway, one key branch of the cysteine and methionine metabolic pathways, could have a pivotal role in pistillody growth involving HTS-1. We found two key enzyme genes in the ethylene synthesis pathway (the SAM synthase gene and the ACC synthase gene) that have higher expression levels in stamens than in pistilloid stamens or pistils. We speculate, that the decrease in ethylene content during stamen development leads to pistillody traits in HTS-1. This study helps elucidate the molecular mechanisms underlying stamen and pistil growth in wheat.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/70/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45421999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The application of an Agrobacterium-mediated in planta transformation system in a Catharanthus roseus medicinal plant 农杆菌介导的植物转化系统在长春花药用植物中的应用

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-12-09 DOI: 10.17221/153/2018-CJGPB

Z. Bahari, Sima Sazegari, A. Niazi, A. Afsharifar

{"title":"The application of an Agrobacterium-mediated in planta transformation system in a Catharanthus roseus medicinal plant","authors":"Z. Bahari, Sima Sazegari, A. Niazi, A. Afsharifar","doi":"10.17221/153/2018-CJGPB","DOIUrl":"https://doi.org/10.17221/153/2018-CJGPB","url":null,"abstract":"The lack of an efficient protocol for the transformation of Catharanthus roseus, as an important medicinal plant, causes obstacles in the genetic engineering programmes of this plant. Therefore, the present study was conducted to examine a convenient and reliable system for generating stable transgenic lines based on the in planta method. To do so, two different in planta Agrobacterium infection systems were investigated. The transgenic lines were produced through ovary injection and shoot apical meristem (SAM) immersing methods. The presence and expression of the GFP transgene in the T0 and T1 lines were confirmed by PCR and RT-PCR. Moreover, the ELISA test confirmed the GFP expression in the transgenic lines. Apart from the superiority of the ovary injection over the SAM inoculation due to the normal growth and healthy appearance, it also showed a significant difference from the point of the confirmed transgenic plant numbers. GFP integration was confirmed in 6 out of 50 T1 plants from the ovary injection and only 3 out of 150 plants generated from the SAM inoculation. In addition to the comparison of these two separate methods, we reached a new convenient in planta system by a 12% transformation rate for generating the C. roseus transgenic lines. According to the result of this study, ovary injection can be introduced as a novel, facile, and stable method for the Catharanthus transformation programme.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"56 1","pages":"34-41"},"PeriodicalIF":0.9,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/153/2018-CJGPB","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45707665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

A breeding concept to improve the performance of locally cultivated bread wheat (Triticum aestivum L.) cultivars 提高地方栽培小麦（Triticum aestivum L.）品种性能的育种理念

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-12-09 DOI: 10.17221/47/2019-cjgpb

V. Greveniotis, S. Zotis, E. Sioki, C. Ipsilandis

{"title":"A breeding concept to improve the performance of locally cultivated bread wheat (Triticum aestivum L.) cultivars","authors":"V. Greveniotis, S. Zotis, E. Sioki, C. Ipsilandis","doi":"10.17221/47/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/47/2019-cjgpb","url":null,"abstract":"The objective of this study was to explore the internal variability in six established F7 commercial wheat cultivars for breeding purposes. They are sown traditionally in the region of Western Macedonia, Greece. Spikes of the six cultivars were sown in 2008 in separate rows. A head to row selection scheme was applied for two growing periods in order to select lines within the cultivars, based on various traits such as: the total spike number, the spike weight per row, the 1000-kernel weight and the specific weight . The final selection was based on the specific weight and the four best rows from each cultivar were selected. All selected lines were tested in a field trial with a randomised complete block design (RCB). The original seed of the cultivars were used as controls. Statistically significant differences were found for all the studied traits. The line selections differed from the original cultivars, sometimes highly significantly. In conclusion, commercial cultivars that are sown traditionally for many years may contain exploitable variability, which reveals, that the continuous selection within cultivars is necessary to avoid cultivar deterioration and to improve the yield and other traits. The results indicate a degeneration of grain yield from 8% to 20%. Although eye-selection restricts off-types, our results mainly indicate new variability and cultivar performance deterioration under extreme biotic and abiotic stress.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"56 1","pages":"1-8"},"PeriodicalIF":0.9,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/47/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47070311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Marker-trait associations of yield related traits in bread wheat (Triticum aestivum L.) under a semi-arid climate 半干旱气候下小麦产量相关性状的标记-性状关联

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-09-23 DOI: 10.17221/154/2018-CJGPB

Salem Marzougui, M. Kharrat, M. Younes

引用次数: 5

Early in vitro selection of winter oilseed rape (Brassica napus L.) plants with the fertility restorer gene for CMS Shaan 2A via non-destructive molecular analysis of microspore-derived embryos 利用小孢子来源胚胎的无损分子分析，筛选具有不育性恢复基因的冬季油菜籽(Brassica napus L.) Shaan 2A植株

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-09-23 DOI: 10.17221/94/2018-cjgpb

M. Klíma, Eva Jozová, Irena Jelínková, V. Kučera, Shengwu Hu, V. Čurn

{"title":"Early in vitro selection of winter oilseed rape (Brassica napus L.) plants with the fertility restorer gene for CMS Shaan 2A via non-destructive molecular analysis of microspore-derived embryos","authors":"M. Klíma, Eva Jozová, Irena Jelínková, V. Kučera, Shengwu Hu, V. Čurn","doi":"10.17221/94/2018-cjgpb","DOIUrl":"https://doi.org/10.17221/94/2018-cjgpb","url":null,"abstract":"Cotyledons of 201 microspore-derived oilseed rape (Brassica napus) embryos were analysed for the presence of the cytoplasmic male sterility Shaan 2A restorer gene using the primer pair 3F/4R. In total, 172 plants regenerated and were grown to the generative stage. Among phenotypically non-haploid flowering plants, there was a broad spectrum of different flower types from normally developed flowers to flowers with short anthers, stamens, or deformed flowers with atypically developed floral organs, often with traces of pollen. For this reason, only 115 typically fertile or sterile accessions were selected for further phenotype-genotype comparisons. In 14 plants (12.2%) the phenotype did not match the genotype: two plants were marked as fertile, but without the Rf gene. Vice versa, in 12 sterile plants the Rf gene was determined. Nevertheless, the two fertile plants did not produce any pods after self-pollination. In total, 47 doubled haploid (DH) lines with good seed yield, carrying the Rf gene were harvested. ","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/94/2018-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42232313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of diversity and resistance of maize varieties to Fusarium spp. causing ear rot in maize under conditions of natural infection 自然感染条件下玉米品种对玉米穗腐病镰刀菌的多样性和抗性评价

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-09-23 DOI: 10.17221/81/2018-CJGPB

Yonggang Li, D. Jiang, Lan Xu, Sixiang Zhang, P. Ji, H. Pan, B. Jiang, Z. Shen

引用次数: 8

An RNA-Seq analysis of the peach transcriptome with a focus on genes associated with skin colour 桃转录组的RNA-Seq分析，重点关注与肤色相关的基因

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-09-23 DOI: 10.17221/90/2018-CJGPB

P. He, Linguang Li, Haibo Wang, Yuansheng Chang

引用次数: 1

Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiency 拟南芥H+-焦磷酸酶的过表达改善了苜蓿在长期盐碱、干旱和缺磷条件下的生长

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-09-23 DOI: 10.17221/134/2018-CJGPB

Jiangshuo Su, T. Bai, Fei Wang, Aike Bao

{"title":"Overexpression of Arabidopsis H+-pyrophosphatase improves the growth of alfalfa under long-term salinity, drought conditions and phosphate deficiency","authors":"Jiangshuo Su, T. Bai, Fei Wang, Aike Bao","doi":"10.17221/134/2018-CJGPB","DOIUrl":"https://doi.org/10.17221/134/2018-CJGPB","url":null,"abstract":"Alfalfa planting is threatened by limited arable land, salinization, water shortage, and soil nutrient deprivation. To deal with this challenge, we previously introduced the Arabidopsis type I H+-pyrophosphatase gene AVP1 into alfalfa and found that transgenic lines exhibited enhanced tolerance to short-term salinity or drought. In this study, the growth performances of two transgenic lines were further investigated under long-term salinity or drought conditions, as well as under phosphate deficiency (low-Pi). Compared with wild-type (WT) plants, the transgenic alfalfa showed better growth performance with taller plants and more biomass accumulation after being treated with either long-term salinity, long-term drought, or low-Pi. Most importantly, the overexpression of AVP1 significantly increased the root dry weight and the root/shoot ratio of transgenic alfalfa. A more robust root system facilitates the transgenic alfalfa to absorb nutrients, and in turn promotes the growth of the plants. Whether being treated with low-Pi or not, transgenic plants showed higher total phosphorus concentrations by 16.5–35.5% than WT plants. This study laid a foundation for breeding alfalfa cultivars adapted to saline, arid and nutrient-deprived marginal land. ","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/134/2018-CJGPB","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48460141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8