Czech Journal of Genetics and Plant Breeding最新文献_第10页

Study of pathogenicity and genetic diversity of Magnaporthe oryzae isolated from rice hybrid Wuyou 308 and detection of resistance genes 水稻杂交种乌优308中稻瘟病菌的致病性和遗传多样性研究及抗性基因检测

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-06-22 DOI: 10.17221/64/2019-cjgpb

Lan Bo, Ying-Qing Yang, S. Qiang, Hongfan Chen, Chen Jian, Yonghui Huang, G. Huo, Xiangmin Li

{"title":"Study of pathogenicity and genetic diversity of Magnaporthe oryzae isolated from rice hybrid Wuyou 308 and detection of resistance genes","authors":"Lan Bo, Ying-Qing Yang, S. Qiang, Hongfan Chen, Chen Jian, Yonghui Huang, G. Huo, Xiangmin Li","doi":"10.17221/64/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/64/2019-cjgpb","url":null,"abstract":"To understand the cause of loss of rice blast resistance, we studied the pathogenicity of Magnaporthe oryzae strains isolated from rice hybrid Wuyou 308 and evaluated its resistance genes. A total of 62 M. oryzae strains were isolated and tested in 7 Chinese rice varieties with varying degrees of resistance to rice blast and 30 blast-resistant monogenic lines. Fourteen physiological races of M. oryzae were identified: 8.55% belonging to the ZA group, 86.67% to the ZB group, and 5.00% to the ZC group. ZB15 was the most abundant race (45.00%). Five resistance genes, Pi-3(1), Pi-z5, Pi-k, Pi-kp(C), and Pi-k(C), conferred good resistance to the 62 strains, with resistance frequencies of 95.56, 91.11, 88.89, 82.22, and 82.22%, respectively. In contrast, Pi-a(2) had a resistance frequency of 0%. The hybrid combination Wuyou 308 was found to carry Pi-ta and Pi-b genes. Because Pi-ta and Pi-b both showed low resistance frequencies to M. oryzae isolated from Jiangxi, the hybrid rice variety Wuyou 308 could be infected by most of the 62 M. oryzae strains. The emergence and spread of rice blast disease in Wuyou 308 may thus be difficult to avoid when climatic conditions are favourable.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2020-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/64/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41704228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The development and validation of new DNA markers linked to the thousand-grain weight QTL in bread wheat (Triticum aestivum L.) 小麦（Triticum aestivum L.）千粒重QTL新DNA标记的建立与鉴定

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-03-17 DOI: 10.17221/35/2019-cjgpb

Congcong Yang, Jian Ma, Cong Li, Min-jie Sun, Yaya Zou, Ting Li, Y. Mu, H. Tang, X. Lan

引用次数: 1

Genetic characterisation of the petal spot phenotype for Gossypium arboreum accession PI 408798 植物棉登录号PI408798花瓣斑点表型的遗传特征

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-03-17 DOI: 10.17221/88/2019-cjgpb

J. Erpelding

引用次数: 4

Genetic diversity of released Malaysian rice varieties based on single nucleotide polymorphism markers 基于单核苷酸多态性标记的马来西亚水稻品种遗传多样性

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-03-17 DOI: 10.17221/58/2019-cjgpb

S. A. Razak, N. Azman, R. Kamaruzaman, S. A. Saidon, Muhammad Fairuz Mohd Yusof, Siti Norhayati Ismail, M. A. Jaafar, Norziha Abdullah

{"title":"Genetic diversity of released Malaysian rice varieties based on single nucleotide polymorphism markers","authors":"S. A. Razak, N. Azman, R. Kamaruzaman, S. A. Saidon, Muhammad Fairuz Mohd Yusof, Siti Norhayati Ismail, M. A. Jaafar, Norziha Abdullah","doi":"10.17221/58/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/58/2019-cjgpb","url":null,"abstract":"Understanding genetic diversity is a main key for crop improvement and genetic resource management. In this study, we aim to evaluate the genetic diversity of the released Malaysian rice varieties using single nucleotide polymorphism (SNP) markers. A total of 46 released Malaysian rice varieties were genotyped using 1536 SNP markers to evaluate their diversity. Out of 1536 SNPs, only 932 SNPs (60.7%) represented high quality alleles, whereas the remainder either failed to amplify or had low call rates across the samples. Analysis of the 932 SNPs revealed that a total of 16 SNPs were monomorphic. The analysis of the SNPs per chromosome revealed that the average of the polymorphic information content (PIC) value ranged from 0.173 for chromosome 12 to 0.259 for chromosome 11, with an average of 0.213 per locus. The genetic analysis of the 46 released Malaysian rice varieties using an unweighted pair group method with arithmetic mean (UPGMA) dendrogram revealed the presence of two major groups. The analysis was supported by the findings from the STRUCTURE analysis which indicated the ∆K value to be at the highest peak at K = 2, followed by K = 4. The pairwise genetic distance of the shared alleles showed that the value ranged from 0.000 (MR159 MR167) to 0.723 (MRIA Setanjung), which suggested that MR159 and MR167 were identical, and that the highest dissimilarity was detected between MRIA 1 and Setanjung. The results of the study will be very useful for the variety identification, the proper management and conservation of the genetic resources, and the exploitation and utilisation in future breeding programmes.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/58/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41578341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

List of field crop varieties registered in the Czech Republic in 2019 2019 年在捷克共和国注册的大田作物品种清单

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-03-17 DOI: 10.17221/19/2020-cjgpb

T. Mezlík

引用次数: 0

A 1BL/1RS translocation contributing to kernel length increase in three wheat recombinant inbred line populations 1BL/1RS易位对三个小麦重组自交系群体粒长增加的贡献

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-03-17 DOI: 10.17221/79/2019-cjgpb

Shuiqin Li, H. Tang, Han Zhang, Y. Mu, X. Lan, Jian Ma

{"title":"A 1BL/1RS translocation contributing to kernel length increase in three wheat recombinant inbred line populations","authors":"Shuiqin Li, H. Tang, Han Zhang, Y. Mu, X. Lan, Jian Ma","doi":"10.17221/79/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/79/2019-cjgpb","url":null,"abstract":"The 1BL/1RS wheat-rye translocation has been widely utilized in wheat genetic improvement and breeding programs. Our understanding on the effects of the 1BL/1RS translocation on wheat kernel size (e.g. length and width) is limited despite of numerous studies reporting about the effects on kernel weight. Here, we identified a wheat 1BL/1RS translocation line 88-1643 with higher kernel length (KL) using fluorescence in situ hybridization (FISH), genomic in situ hybridization (GISH) and molecular markers. To detect the possible role of the 1BL/1RS translocation in KL, kernel width (KW), and thousand-kernel weight (TKW), three recombinant inbred line (RIL) populations were constructed by crossing 88-1643 and three other wheat lines. As expected, the results showed that the values of KL in lines carrying 1RS were significantly higher than those carrying 1BS in three RIL populations at multiple environments, indicating that a major and stably expressed allele or gene responsible for increasing KL is most likely located on 1RS from 88-1643. Additionally, in one RIL population, the increased KL contributed significantly to the increase in TKW. Collectively, the 1BL/1RS translocation reported here is of interest to reveal molecular mechanism of the gene controlling KL and will be useful for improving wheat yield.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/79/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46534818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Ing. Pavel Bartoš, DrSc. − 90th birthday Pavel Bartoš，博士90岁生日

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-03-17 DOI: 10.17221/21/2020-cjgpb

A. Hanzalová, Hana Reimarová

引用次数: 0

Identification of rare traditional grapevine cultivars using SSR markers and their geographical location within the Czech Republic 利用SSR标记鉴定捷克共和国境内稀有传统葡萄品种及其地理位置

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-03-17 DOI: 10.17221/61/2019-cjgpb

K. Baránková, R. Sotolář, M. Baránek

引用次数: 3

Comprehensive genomic analysis and expression profiling of the BTB and TAZ (BT) genes in cucumber (Cucumis sativus L.) 黄瓜BTB和TAZ（BT）基因的综合基因组分析及表达谱

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-12-09 DOI: 10.17221/34/2019-cjgpb

Yong Zhou, Guanghua Li, Lin Zhang, Jie Xu, Lifang Hu, Lunwei Jiang, Shiqiang Liu

引用次数: 6

Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCR 冬小麦品种对眼斑病反应的目测和实时PCR分析

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2019-12-09 DOI: 10.17221/52/2019-cjgpb

J. Palicová, P. Matušinský, A. Hanzalová, I. Svačinová, V. Dumalasová, J. Chrpová

{"title":"Reaction of winter wheat cultivars to eyespot assessed visually and by real-time PCR","authors":"J. Palicová, P. Matušinský, A. Hanzalová, I. Svačinová, V. Dumalasová, J. Chrpová","doi":"10.17221/52/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/52/2019-cjgpb","url":null,"abstract":"The reaction of twelve winter wheat cultivars frequently grown in the Czech Republic and twenty-five new breeding lines to inoculation with Oculimacula yallundae and Oculimacula acuformis was evaluated in small plot trials from 2017–2018. The assessment was carried out visually by symptoms and by a quantitative real-time polymerase chain reaction (qPCR). The aims of the study were to compare the results of both methods, to evaluate the effect of the resistance gene Pch1 to eyespot, and to select new breeding lines resistant to eyespot. The relationship between the eyespot symptoms and the pathogen DNA content in plant tissues followed a moderate linear regression. Low levels of eyespot were observed in the cultivars/lines possessing the resistance gene Pch1 (Annie, Hermann, Rebell, SG-S1215-14, SG-S1825-14, SG-S791-13) and also in the line SG-SU630-15. The qPCR method was able to detect low levels of the pathogens in the plant tissue and to distinguish two eyespot pathogens. O. acuformis was detected in very low concentrations in the inoculated plants compared with O. yallundae. The eyespot infection rate was significantly higher in 2017 than in the next agricultural season due to extremely dry and warm spring weather in 2018.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2019-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/52/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43309974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3