应用全基因组测序技术构建烟草回交BC4F3群体高密度遗传图谱及QTL定位

IF 1.2 4区农林科学 Q3 AGRONOMY

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-06-08 DOI:10.17221/8/2021-CJGPB

Zhijun Tong, Sanjie Jiang, Weiming He, Xue-Jun Chen, Lixin Yin, D. Fang, Yafei Hu, F. Jiao, Chi Zhang, Zeng Jianmin, Xin-yan Wu, Shancen Zhao, Jianbo Jian, B. Xiao

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引用次数: 1

摘要

回交是植物育种的有力工具。改良的标记辅助回交旨在将供体亲本中感兴趣的目标基因或数量性状位点(qtl)转移到复发亲本中。本研究以Y3和K326为杂交亲本，YF1-1为F1亲本，构建烟草BC4F3群体。利用381个家系群体的高通量测序数据，构建了包含24 142个高质量SNP (single nucleotide polymorphism)标记的高密度遗传图谱，平均遗传距离为0.59 cM。然后对所有后代进行基因组模块分析。共鉴定出6个农艺性状的43个候选qtl。该研究为烟草育种提供了原始的生物标记物，并为其他植物回交应用提供了线索。

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Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing

Backcrossing is a powerful tool for plant breeding. The improved marker-assisted backcrossing intends to transfer targeted genes or quantitative trait loci (QTLs) of interest from a donor parent into a recurrent parent. In this study, a tobacco BC4F3 population was generated using Y3 and K326 as hybrid parents and YF1-1 as F1 parents. High-throughput sequencing data of 381 pedigree populations were used to construct high-density genetic maps containing 24 142 high-quality single nucleotide polymorphism (SNP) markers with an average genetic distance of 0.59 cM. A genome module analysis was then performed for all the offspring. A total of forty-three candidate QTLs for six agronomics traits were identified. This study provides original biomarkers for tobacco breeding and offers clues for prospective backcrossing applications in other plants.

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来源期刊

Czech Journal of Genetics and Plant Breeding Agricultural and Biological Sciences-Plant Science

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Original scientific papers, critical reviews articles and short communications from the field of theoretical and applied plant genetics, plant biotechnology and plant breeding. Papers are published in English.