Czech Journal of Genetics and Plant Breeding最新文献_第7页

Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley 西藏无壳大麦HvnHID基因的分离、测序及其在紫色粒色发育中的作用

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-10-05 DOI: 10.17221/34/2021-cjgpb

X. Yao, Leping Su, Youhua Yao, Li-Gang An, Yixiong Bai, Xin Li, Kunlun Wu

{"title":"Isolation, sequencing of the HvnHID gene and its role in the purple-grain colour development in Tibetan hulless barley","authors":"X. Yao, Leping Su, Youhua Yao, Li-Gang An, Yixiong Bai, Xin Li, Kunlun Wu","doi":"10.17221/34/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/34/2021-cjgpb","url":null,"abstract":"2-hydroxyisoflavanone dehydratase (HID) plays an important role in isoflavone biosynthesis. In this study, HID was isolated from the seeds of the purple-grained Tibetan hulless barley variety Nerumuzha and the white-grained variety Kunlun 10. The HvnHID gene includes the 981 bp open reading frame and encodes a protein of 327 amino acids. It has a typical Abhydrolase_3 domain (78–306) and belongs to the carboxylesterase (CXE) family of the Abhydrolase_3 (α/β hydrolase) superfamily. There are eight nucleotide differences in the HvnHID coding sequence and two amino acid differences (one in the Abhydrolase_3 domain) between Nerumuzha and Kunlun 10. The HvnHID of hulless barley has the closest relationship with the HID in Hordeum vulgare, and the most distant relationship in Panicum hallii. At the early-mid stage of the seed colour development, the HvnHID expression levels in the purple and black seeds were significantly higher than in the white and blue ones (P < 0.01). During the seed colour development of purple-grained hulless barley, the expression of the key genes (HvnF3'H, HvnDRF, HvnANT1, and HvnGT) in the anthocyanidin biosynthetic pathway increased significantly, while the HvnHID expression decreased significantly (P < 0.01). Thus, it is likely that HvnHID negatively regulates the anthocyanidin biosynthesis. This result provides an important basis for further study of the biological functions of HvnHID in the anthocyanidin biosynthetic pathway.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43193919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparing the number of Iranian pomegranate genotypes based on morphological and biochemical properties 基于形态和生化特性的伊朗石榴基因型数量比较

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-09-14 DOI: 10.17221/39/2021-cjgpb

S. Yari, S. Mirjalili, A. Mousavi, E. Poorazizi

{"title":"Comparing the number of Iranian pomegranate genotypes based on morphological and biochemical properties","authors":"S. Yari, S. Mirjalili, A. Mousavi, E. Poorazizi","doi":"10.17221/39/2021-cjgpb","DOIUrl":"https://doi.org/10.17221/39/2021-cjgpb","url":null,"abstract":"Punica granatum L. is one of the oldest known edible fruits. Numerous chemical compounds have been isolated from pomegranate seeds, juice, and peels, which have beneficial effects on human health. This study aimed to perform the physicochemical and morphological properties of twenty-four pomegranate genotypes from various provinces of Iran. Fifteen fruits of each cultivar are collected at harvest maturity in the normal ripening period for the pomegranate from the Iranian pomegranate genetic collection in Yazd, Iran. Five fruits were randomly harvested from each of four orientations of the tree, and were immediately taken to the laboratory for analysis. Three replicates were maintained for each analysis. The results indicated the highest levels of anthocyanin was observed in S783 and R633, while polyphenols in Q529, the antioxidant capacity in N755 and the total soluble solids levels in R633 and the total acidity levels were found in K477 and E336. On the other hand, the fruit weight (in S948), fruit diameter (in SH1738), crown diameter (in R533), total weight of the seeds (in S948), peel thickness (in S716), peel colour (in S948), and red juice (in S783) are significantly affected by the genotype. At a similarity of 50%, the genotypes were divided into nine sub-clusters including A, B, C, D, E, F, G, H and I. These identified genotypes can be rolled out in future breeding programmes.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42530412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Development of the new waxy winter wheat cultivars Eldija and Sarta 糯性冬小麦新品种Eldija和Sarta的选育

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-08-17 DOI: 10.17221/37/2021-CJGPB

Ž. Liatukas, V. Ruzgas, A. Gorash, J. Cecevičienė, R. Armonienė, G. Statkevičiūtė, K. Jaškūnė, G. Brazauskas

{"title":"Development of the new waxy winter wheat cultivars Eldija and Sarta","authors":"Ž. Liatukas, V. Ruzgas, A. Gorash, J. Cecevičienė, R. Armonienė, G. Statkevičiūtė, K. Jaškūnė, G. Brazauskas","doi":"10.17221/37/2021-CJGPB","DOIUrl":"https://doi.org/10.17221/37/2021-CJGPB","url":null,"abstract":"Two new waxy winter wheat (Triticum aestivum L.) cultivars, Eldija and Sarta, were developed at the Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry and released in Lithuania in 2021. The cultivars were developed using waxy wheat material from Nebraska, the United States of America. The mean yield of Eldija and Sarta at five locations over three testing years was 7.56 and 7.21 t/ha or 79.63 and 75.95%, respectively, compared to the yield of the standard cultivars. Eldija and Sarta should be grown under high input conditions due to the relatively low resistance to leaf spot diseases and Fusarium head blight and medium tolerance to lodging. An amylose content of 0.68% and 0.36% of Eldija and Sarta, respectively, a very low falling number (about 60 s), a lower flour yield and higher water absorption compared to common wheat and the reaction to iodine staining (brown colour) characterised the new cultivars as fully waxy wheats. These cultivars are intended for the potential demand from commercial companies for special use in the food industry.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48111964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Association of leaf chlorophyll content with the stay-green trait and grain yield in wheat grown under heat stress conditions 热胁迫条件下小麦叶片叶绿素含量与保绿特性及产量的关系

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-07-16 DOI: 10.17221/45/2021-CJGPB

C. Roy, T. Chattopadhyay, R. Ranjan, Wahid ul Hasan, Abhishek Kumar, Nitish De

{"title":"Association of leaf chlorophyll content with the stay-green trait and grain yield in wheat grown under heat stress conditions","authors":"C. Roy, T. Chattopadhyay, R. Ranjan, Wahid ul Hasan, Abhishek Kumar, Nitish De","doi":"10.17221/45/2021-CJGPB","DOIUrl":"https://doi.org/10.17221/45/2021-CJGPB","url":null,"abstract":"Heat stress is a major production constraint of wheat in South Asia, particularly in the Gangetic plains of India and Bangladesh. The leaf chlorophyll status is a key determinant for a high rate of photosynthesis under stress. The present experiments included 238 genotypes in 2016–2017 and 321 genotypes in 2017–2018 under optimum and under heat stress conditions. Subsequently, a set of 100 genotypes selected on basis of the heat susceptibility index was evaluated in 2018–2019 under heat stress conditions to study the relationship between important physiological traits and yield under stress. A significant correlation of soil plant analysis development (SPAD) value of the two upper leaves with stay-green trait and grain yield indicates the importance of chlorophyll content, both in flag and penultimate leaf, in maintaining leaf areas under greenness (LAUG) and grain yield under heat stress. The SPAD in the flag and penultimate leaf was responsible for 8.8% and 10.9%, respectively, of the variation in grain yield. For the stay-green trait, 8.4% and 7.2 % of the variation was governed by the SPAD value in the flag and penultimate leaf, respectively. These results suggest that, in addition to the flag leaf, the chlorophyll status of the penultimate leaf can be an important criterion for the selection of superior wheat genotypes under heat stress. The genotypes SW-139; SW 108; DWR-F8-35-9-1; NHP-F8-130; DWR-F8-3-1 that maintained a high chlorophyll content in the flag and penultimate leaf can be used further in breeding programmes addressing heat resistance in wheat.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43194084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Mapping of quantitative trait loci for purple stigma and purple apiculus in rice by using a Zhenshan 97B/Minghui 63 RIL population 水稻紫色柱头和紫色尖数量性状位点的珍山97B/明会63 RIL群体定位

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-07-14 DOI: 10.17221/20/2021-CJGPB

Jiping Tong, Zhengshu Han, A. Han

{"title":"Mapping of quantitative trait loci for purple stigma and purple apiculus in rice by using a Zhenshan 97B/Minghui 63 RIL population","authors":"Jiping Tong, Zhengshu Han, A. Han","doi":"10.17221/20/2021-CJGPB","DOIUrl":"https://doi.org/10.17221/20/2021-CJGPB","url":null,"abstract":"Anthocyanin pigmentation is an important morphological marker that is commonly used to identify rice varieties and for linkage analysis. The following study investigates the genetic factors involved in the purple stigma (Ps) and purple apiculus (Pa) traits of an important indica rice cross between Zhenshan 97 (purple stigma and purple apiculus) and Minghui 63 (grey stigma and colourless apiculus). A recombinant inbred line (RIL) population derived from this cross was used for quantitative trait loci (QTL) mapping of the purple stigma and purple apiculus traits. As a result, one major QTL for the purple stigma trait, temporarily designated qPS-1-1, and one major QTL for the purple apiculus trait, temporarily designated qPA-1-1, were mapped to the short arm of chromosome 6 in the interval between the two markers Y4073L and *P. The LOD peaks of qPS-1-1 and qPA-1-1 were 44.0127 and 173.3585, respectively. In addition, qPS-1-1 and qPA-1-1 explained 66.7416% and 98.6441% of the total phenotypic variance, respectively. The Zhenshan 97 allele increased the purple stigma trait by approximately 8.0355% (for qPS-1-1) and 9.8863% (for qPA-1-1). Moreover, since qPS-1-1 and qPA-1-1 were strongly correlated, they were also located in the same vicinity of the C gene on the short arm of chromosome 6, which suggested that the two QTL might be the same. By comparing these and previous results, it was deduced that qPS-1-1 or qPA-1-1 was the C gene and was pleiotropic for both the colouration of the apiculus and the colouration of the stigma in rice.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46659638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

TPR domain coding gene ST2 may be involved in regulating tillering and fertility in rice TPR结构域编码基因ST2可能参与水稻分蘖和育性调控

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-07-14 DOI: 10.17221/103/2020-CJGPB

G. Wei, Hongxia Yang, Zixian Xiong, Jingwen Wu, Danyang Chen, Yang Liu, Yijie Ban, Weichi Liu, Lina Shang, Nan Wang

{"title":"TPR domain coding gene ST2 may be involved in regulating tillering and fertility in rice","authors":"G. Wei, Hongxia Yang, Zixian Xiong, Jingwen Wu, Danyang Chen, Yang Liu, Yijie Ban, Weichi Liu, Lina Shang, Nan Wang","doi":"10.17221/103/2020-CJGPB","DOIUrl":"https://doi.org/10.17221/103/2020-CJGPB","url":null,"abstract":"A decrease in the tiller number and male sterility will lead to a decline in the rice yield. Therefore, it is significant to study the molecular mechanism of controlling the tiller number and regulating the male reproductive development. The mutant st2 (single tiller 2) was induced by ethyl methane sulfonate (EMS) in the indica maintainer line Xinong 1B and showed single tillering and male sterility. I2-KI staining showed that the st2 pollen was aborted. The scanning electron microscope (SEM) observation underlined that the anther of st2 became smaller, the wax of the epidermis reduced, the inner wall shrank and the Ubisch body decreased, the pollen collapsed, and the germination pore developed abnormally. The genetic analysis discovered that the trait was controlled by a single recessive nuclear gene located on chromosome 3. LOC_Os03g05540 encoding a tetratricopeptide repeat (TPR) domain was identified as the candidate gene by sequencing. The quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that ST2 was highly expressed in the stem apical meristem (SAM) and the initial stage of meiosis during the anther development. The subcellular localisation indicated that ST2 is a nuclear and plasmic localisation protein. The homology analysis demonstrated that ST2 was evolutionarily conserved. These results laid a foundation for further study of the ST2 function.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41680803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Construction of high-density genetic map and QTL mapping in Nicotiana tabacum backcrossing BC4F3 population using whole-genome sequencing 应用全基因组测序技术构建烟草回交BC4F3群体高密度遗传图谱及QTL定位

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-06-08 DOI: 10.17221/8/2021-CJGPB

Zhijun Tong, Sanjie Jiang, Weiming He, Xue-Jun Chen, Lixin Yin, D. Fang, Yafei Hu, F. Jiao, Chi Zhang, Zeng Jianmin, Xin-yan Wu, Shancen Zhao, Jianbo Jian, B. Xiao

引用次数: 1

Genome-wide identification and analysis of the MLO gene families in three Cucurbita species 三种葫芦属植物MLO基因家族的全基因组鉴定与分析

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-05-06 DOI: 10.17221/99/2020-CJGPB

Lei Zhu, Yanman Li, Jintao Li, Yong Wang, Zhenli Zhang, Yanjiao Wang, Zan Wang, Jianbin Hu, Yang Luming, Sun Shouru

引用次数: 4

Identification of known leaf rust resistance genes in bread wheat cultivars from China 中国面包小麦品种已知抗叶锈病基因的鉴定

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-05-06 DOI: 10.17221/6/2021-CJGPB

Xiaocui Yan, T. Gebrewahid, Ruibin Dong, Xing Li, Pei-pei Zhang, Zhanjun Yao, Zaifeng Li

{"title":"Identification of known leaf rust resistance genes in bread wheat cultivars from China","authors":"Xiaocui Yan, T. Gebrewahid, Ruibin Dong, Xing Li, Pei-pei Zhang, Zhanjun Yao, Zaifeng Li","doi":"10.17221/6/2021-CJGPB","DOIUrl":"https://doi.org/10.17221/6/2021-CJGPB","url":null,"abstract":"Leaf rust caused by Puccinia triticina Eriks. (Pt) is one of the most devastating fungal pathogens affecting wheat (Triticum aestivum L.) production worldwide. Deployment of resistant cultivars is the most environmentally friendly approach to control the disease. In this study, thirty-seven wheat lines from the Hubei and Shaanxi provinces in China were evaluated for seedling resistance in the greenhouse using eighteen Pt races. These lines were also tested for slow rusting resistance in the field in the 2014 to 2018 growing seasons. Eleven molecular markers closely associated with known Lr genes were used as part of the postulation process. Seven known Lr genes, 1, 13, 18, 14a, 26, 34 and 46 either singly or in combination were postulated in twenty-five cultivars. Lr1 and Lr26 were the most commonly identified genes detected in thirteen and ten cultivars, respectively. Lr13 and Lr46 were each found in four and five cultivars. Lr34 was present in three cultivars. Lr18 and Lr14a were identified in cultivar Xi’nong 538. Six cultivars displayed slow rusting resistance in the field tests. The resistant cultivars identified in the present study can be used as resistance parents in crosses aimed at pyramiding and the deployment of leaf rust resistance genes in China.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44389496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effect of terminal heat stress on physiological traits, grain zinc and iron content in wheat (Triticum aestivum L.) 末热胁迫对小麦生理性状及籽粒锌、铁含量的影响

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2021-04-09 DOI: 10.17221/63/2020-CJGPB

M. Narendra, C. Roy, Sudhir Kumar, P. Virk, Nitish De

{"title":"Effect of terminal heat stress on physiological traits, grain zinc and iron content in wheat (Triticum aestivum L.)","authors":"M. Narendra, C. Roy, Sudhir Kumar, P. Virk, Nitish De","doi":"10.17221/63/2020-CJGPB","DOIUrl":"https://doi.org/10.17221/63/2020-CJGPB","url":null,"abstract":"Heat stress is one of the major wheat (Triticum aestivum) production constraints in South Asia (SA), particularly in the Eastern Gangetic Plains (EGP) of India and Bangladesh. Malnutrition is also a severe problem among children and women in SA. Wheat varieties with high grain Zn/Fe are a sustainable, cost-effective solution in the fight against hidden hunger. Thirty wheat genotypes were characterised under the optimum temperature and heat stress conditions in 2016–2017 and 2017–2018 to study the response of the stress on the yield, physiological traits and grain Zn/Fe content. A significant genetic variation was observed for all the traits under the optimum temperature and stress conditions. The yield was reduced by an average of 59.5% under heat stress compared to that of the optimum temperature. A strong positive association of the canopy temperature depression (CTD) with the grain yield (GY) was observed under the heat stress. A negative correlation of the grain Zn/Fe with the yield was observed under the optimum temperature and heat stress conditions, while the association between the grain Zn and Fe was positive. The genotypes BRW 3723, BRW 3759, BRW 3797, BRW 160, HD 2967, HD 2640 were found to be heat-tolerant in both years. Among the tolerant genotypes, BRW 934, BRW 3807 and BRW 3804 showed a high zinc content and BRW 934, BRW 3797, BRW 3788 and BRW 3807 showed a high iron content, respectively. These genotypes can be explored in future breeding programmes to address the problem of nutritional deficiency.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2021-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41955390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14