Czech Journal of Genetics and Plant Breeding最新文献_第8页

QTL mapping of adult plant resistance to stripe rust in the Fundulea 900 × Thatcher RIL population Fundulea 900×Thatcher群体成株抗条锈病QTL定位

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-11-02 DOI: 10.17221/71/2020-cjgpb

Xiaocui Yan, Huimin Zheng, Pei-pei Zhang, Gebrewahid Takele Weldu, Zaifeng Li, Daqun Liu

{"title":"QTL mapping of adult plant resistance to stripe rust in the Fundulea 900 × Thatcher RIL population","authors":"Xiaocui Yan, Huimin Zheng, Pei-pei Zhang, Gebrewahid Takele Weldu, Zaifeng Li, Daqun Liu","doi":"10.17221/71/2020-cjgpb","DOIUrl":"https://doi.org/10.17221/71/2020-cjgpb","url":null,"abstract":"Wheat stripe rust, caused by Puccinia striiformis Westend. f.sp. tritici Eriks (Pst), is one of the most important diseases of bread wheat worldwide. Breeding resistant wheat cultivars is the most economical, effective and environmentally friendly way for controlling wheat stripe rust in China. The Romanian wheat line Fundulea 900 showed good resistance to wheat stripe rust at the adult stage. The present study aimed to map the quantitative trait loci (QTLs) for stripe rust resistance in 176 F2:6 recombinant inbred lines (RIL) derived from the cross of Fundulea 900 × Thatcher. The RIL population was phenotyped for stripe rust (YR) severity at Mianyang in the Sichuan province and Baoding in the Hebei province in the 2016/2017 and 2017/2018 cropping seasons. SSR markers combined with a preferred screened group (PSG) analysis were used to identify the QTLs for stripe rust in the population. Three QTLs for stripe rust resistance were mapped on chromosomes 1AL, 7BL and 7DS, respectively. All three QTLs originated from Fundulea 900 and were detected in all the environments. The QTL on 7DS was provided by the known resistance gene Yr18/Lr34. The two QTLs on chromosomes 1AL and 7BL were explained by 9.2 to 21.5% and 5.1 to 10.1% of the phenotypic variance, respectively and might be new QTLs. The QTLs identified in the study and their closely linked markers can be used for marker-assisted selection (MAS) in wheat breeding programmes.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2020-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48871137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Changes in the expression of CrFTA, the Catharanthus roseus farnesyltransferase α-subunit gene, in response to a Candidatus Liberibacter asiaticus infection 花楸法尼基转移酶α-亚基基因CrFTA在亚洲解放候选杆菌感染后的表达变化

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-11-02 DOI: 10.17221/13/2020-cjgpb

Ya Li, Qinhan Yu

{"title":"Changes in the expression of CrFTA, the Catharanthus roseus farnesyltransferase α-subunit gene, in response to a Candidatus Liberibacter asiaticus infection","authors":"Ya Li, Qinhan Yu","doi":"10.17221/13/2020-cjgpb","DOIUrl":"https://doi.org/10.17221/13/2020-cjgpb","url":null,"abstract":"The farnesyltransferase α-subunit (FTA) may be involved in the regulation of defence responses against pathogens in plants. In this study, this gene was amplified from Catharanthus roseus (CrFTA gene). The cDNA was found to be 1 403 bp long, and encodes a putative protein of 331 amino acids that contains a conserved PPTA motif. The phylogenetic analysis showed that the sequence of CrFTA is the most similar to that from Coffea canephora. The qRT-PCR assays indicated that CrFTA is expressed in the leaves, stems, and roots. During a Candidatus Liberibacter asiaticus (Ca. L. asiaticus) infection, the CrFTA expression levels significantly increased and reached 18-fold that measured in the control group, after which its expression decreased gradually from 22 days after top-grafting (DAT) to the end of the experiment. Spray application of Manumycin A (ManuA), a specific inhibitor of farnesyltransferase, on the leaves of C. roseus plants caused a significant decrease in the CrFTA expression and a significant increase in the Ca. L. asiaticus positivity percentage after top-grafting with the Ca. L. asiaticus-infected shoots compared with the groups not treated with ManuA. Furthermore, ABA had no significant effect on the relative expression of CrFTA and the number of Ca. L. asiaticus-positive plants. These results suggest that CrFTA most likely plays a role in mediating the tolerance to a Ca. L. asiaticus infection in C. roseus.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2020-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48495980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture 以冬小麦(Triticum aestivum L.)育种材料为材料，利用离体花药培养产生双倍单倍体系

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-09-04 DOI: 10.17221/113/2019-cjgpb

Osama Zuhair Kanbar, C. Lantos, P. Chege, E. Kiss, J. Pauk

{"title":"Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture","authors":"Osama Zuhair Kanbar, C. Lantos, P. Chege, E. Kiss, J. Pauk","doi":"10.17221/113/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/113/2019-cjgpb","url":null,"abstract":"We investigated the anther culture (AC) efficiency of thirteen F4 combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25–87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"1 1","pages":"150-158"},"PeriodicalIF":0.9,"publicationDate":"2020-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/113/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45072919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Assessment of epigenetic methylation changes in hop (Humulus lupulus) plants obtained by meristem culture 分生组织培养啤酒花表观遗传甲基化变化的评价

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-08-19 DOI: 10.17221/27/2020-cjgpb

J. Patzak, A. Henychová, P. Svoboda, I. Malířová

{"title":"Assessment of epigenetic methylation changes in hop (Humulus lupulus) plants obtained by meristem culture","authors":"J. Patzak, A. Henychová, P. Svoboda, I. Malířová","doi":"10.17221/27/2020-cjgpb","DOIUrl":"https://doi.org/10.17221/27/2020-cjgpb","url":null,"abstract":"In vitro meristem cultures have been used for the production of hop (Humulus lupulus L.) virus-free rootstocks worldwide, because multipropagation is considered to preserve the genetic stability of the produced plantlet. Nevertheless, in vitro tissue cultures can cause genetic and epigenetic changes. Therefore, we studied the genetic and epigenetic variability of Saaz Osvald’s clones, Sládek and Premiant cultivars on the DNA methylation level by methylation-sensitive amplification polymorphism (MSAP). In vitro propagated plants, acclimatised glasshouse rootstocks as well as derived mericlones and control plants under field conditions were used for the analyses. A total of 346 clearly and highly reproducible amplified products were detected in the MSAP analyses within the studied hop plants. We found 16 polymorphic products (4.6% of products) and 64 products with methylation changes (18.5% of products) in the analyses. The demethylation events were comparable to the de novo methylation events. Most demethylation changes were found in the in vitro plants, but only a few of them were found in the derived mericlones under field conditions. In contrast, the de novo methylation changes persisted in the acclimatised plants under glasshouse or field conditions. A hierarchical cluster analysis was used for the evaluation of the molecular genetic variability within the individual samples. The dendrogram showed that the individual samples of the same variety, more or less, clustered together. Because the methylation status varied during the virus-free rootstock production process, we suppose that de/methylation process is a natural tool of epigenetics and evolution in vegetatively propagated plants.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"1 1","pages":"159-164"},"PeriodicalIF":0.9,"publicationDate":"2020-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/27/2020-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47863482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Rabies virus glycoprotein produced in Nicotiana benthamiana is an immunogenic antigen in mice 本烟中产生的狂犬病毒糖蛋白是一种小鼠免疫原性抗原

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-08-19 DOI: 10.17221/25/2020-cjgpb

Youngmin Park, Hyangju Kang, Kyungmin Min, Nam-Hyung Kim, Minhee Park, I. Ouh, Ha-Hyun Kim, Jae-Young Song, Dong-Kun Yang, E. Sohn, Sangmin Lee

{"title":"Rabies virus glycoprotein produced in Nicotiana benthamiana is an immunogenic antigen in mice","authors":"Youngmin Park, Hyangju Kang, Kyungmin Min, Nam-Hyung Kim, Minhee Park, I. Ouh, Ha-Hyun Kim, Jae-Young Song, Dong-Kun Yang, E. Sohn, Sangmin Lee","doi":"10.17221/25/2020-cjgpb","DOIUrl":"https://doi.org/10.17221/25/2020-cjgpb","url":null,"abstract":"Rabies remains an infectious disease among humans and animals, and requires the development of an effective vaccine essential to prevent rabies. Advances in molecular biology and biotechnology have led to the development and improvement of many rabies vaccines. Before the third-generation of the vaccine, rabies vaccines were based on the virus itself. Thus, even if effective, these vaccines may not be completely safe, resulting in a strong demand for the development of effective subunit vaccines that do not raise concerns about virus replication and infection in the host. This study investigated the ability of the glycoprotein of the rabies virus to be expressed in tobacco plants (Nicotiana benthamiana) and to induce an immune response in mice. Using a transient transfection, a soluble glycoprotein was successfully expressed in N. benthamiana. Fusing of five histidine residues at the C-terminus enabled the glycoprotein to be easily purified by affinity chromatography. The glycoprotein expressed in the plants was found to be N-glycosylated post-translationally, and the mice immunised with this glycoprotein generated neutralising antibodies against the rabies virus. These results suggest that a glycoprotein produced in the endoplasmic reticulum of N. benthamiana is bioactive, and might be used to generate a subunit vaccine against the rabies virus.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2020-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/25/2020-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44622708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Potentials to breed for improved fibre digestibility in temperate Czech maize (Zea mays L.) germplasm 提高温带捷克玉米（Zea mays L.）种质纤维消化率的育种潜力

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-07-21 DOI: 10.17221/11/2020-cjgpb

M. Schönleben, J. Mentschel, L. Střelec

{"title":"Potentials to breed for improved fibre digestibility in temperate Czech maize (Zea mays L.) germplasm","authors":"M. Schönleben, J. Mentschel, L. Střelec","doi":"10.17221/11/2020-cjgpb","DOIUrl":"https://doi.org/10.17221/11/2020-cjgpb","url":null,"abstract":"Cell wall digestibility is an important quality trait of modern silage maize cultivars. The symbiotic relationship between microbes and ruminant livestock enables the efficient upcycling of otherwise for human consumption unsuitable rumen digestible fibre or cell wall components into highly nutritious milk and meat. Before entering the Czech National List of Plant Varieties, new silage maize germplasm is extensively tested for different cell wall digestibility parameters. Recently published, the undigestible neutral detergent fibre (uNDF) cell wall digestibility approach promises even greater practical relevance. The aim of our study was, therefore, to assess the potential of the uNDF method, compared with current standard procedures, using a vast set of official Czech plant variety trial evaluations and Czech silage analyses from the 2018 cropping season. The uNDF method yielded a twice as high phenotypic standard deviation, compared with the current standard approaches. This is good news for plant breeders, official variety testing organisations, and farm professionals alike, enabeling faster variety improvement and simpler variety selection. On the other hand, due to the low differentiation potential, we discourage the use of the absolute lignin content when selecting for digestible silage maize varieties. Since between the digestibility traits enzymatic soluble organic substance (ELOS) and cellulase digestibility (DCS), a Pearson correlation close to one was observed, the substitution of one of these analytics by the uNDF method, may render valuable additional information in a highly economical manner.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"1 1","pages":"133-139"},"PeriodicalIF":0.9,"publicationDate":"2020-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/11/2020-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47223255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Discrimination of tobacco cultivars using SCAR and RAPD markers SCAR和RAPD标记在烟草品种鉴别中的应用

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-07-20 DOI: 10.17221/120/2019-cjgpb

Jiuzhen Sun, Junting Wang, Su Dongying, Yang Jinchu, Wang Erbin, Shixi Wu, Li Meng, Lin Ma

{"title":"Discrimination of tobacco cultivars using SCAR and RAPD markers","authors":"Jiuzhen Sun, Junting Wang, Su Dongying, Yang Jinchu, Wang Erbin, Shixi Wu, Li Meng, Lin Ma","doi":"10.17221/120/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/120/2019-cjgpb","url":null,"abstract":"Tobacco genetic purity is crucial to maintain the quality of cigarette products in the tobacco industry. To reduce the difficulties in the discrimination of large number of tobacco cultivars in production practice, we developed a two-step identification strategy by using SCAR and RAPD markers. A total of 53 tobacco cultivars were examined in the study. Initially, all the selected cultivars were divided into four groups, each group consisted of seven to seventeen tobacco cultivars based on difference in phenotypes identified by the SCAR markers S4 and S8. Later, in each group, each tobacco cultivar was identified using RAPD fingerprinting by using one to four polymorphic primers, which were selected from 200 random primers. The results showed that all 53 tobacco cultivars could be effectively distinguished by using only two SCAR and seven RAPD markers. The two-step fingerprinting strategy could be used as a convenient and cost-effective tool to discriminate large numbers of tobacco cultivars for production planning in the tobacco industry.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"1 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2020-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/120/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41823353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Molecular screening of domestic apple cultivars for scab resistance genes in Greece 希腊国产苹果品种抗赤霉病基因的分子筛选

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-07-20 DOI: 10.17221/119/2019-cjgpb

L. Karapetsi, I. Nianiou-Obeidat, A. Zambounis, M. Osathanunkul, P. Madesis

引用次数: 1

Phenotypic traits for wild red clover seed yield under drought conditions 干旱条件下野生红三叶草种子产量的表型性状

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-07-20 DOI: 10.17221/111/2019-cjgpb

G. Petrauskas, E. Norkeviciene, V. Stukonis, V. Kemešytė

{"title":"Phenotypic traits for wild red clover seed yield under drought conditions","authors":"G. Petrauskas, E. Norkeviciene, V. Stukonis, V. Kemešytė","doi":"10.17221/111/2019-cjgpb","DOIUrl":"https://doi.org/10.17221/111/2019-cjgpb","url":null,"abstract":"Changes in the gene pool and homogeneity of red clover cultivars occur over time. Therefore, it is necessary to constantly renew the breeding material and foundation seed. Moreover, the market also prescribes a new demand. Based on the Natura 2000 habitats profile, we collected seeds from 39 locations across Lithuania in 2016. The phenotypic traits that affect the seed yield of red clover were analysed in 2018. The homogeneity of the populations with reference to the seed yield and 1 000 seed weight (TSW) were determined as well. Also, the possibility of obtaining two seed yields per season under drought conditions from wild genotypes of red clover was analysed. We found that the final seed yield mostly depends on the seed number per flower head (SN/FH), which strongly correlated with 1st component of PCA during first (r = 0.91) and second (0.92) harvest. Meanwhile, the cluster analysis showed that the typical wild red clover has a lower seed weight than the cultivars and could be clustered on the basis of seed homogeneity. Finally, based on the seed phenotype and harvest components, there were five prospective accessions (2177, 2871, 2876, 2898 and 2899) for a new cultivar prototype.","PeriodicalId":50598,"journal":{"name":"Czech Journal of Genetics and Plant Breeding","volume":"1 1","pages":"140-149"},"PeriodicalIF":0.9,"publicationDate":"2020-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.17221/111/2019-cjgpb","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45916378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Wheat leaf rust (Puccinia triticina Eriks.) virulence frequency and detection of resistance genes in wheat cultivars registered in the Czech Republic in 2016–2018 2016-2018年捷克注册小麦品种小麦叶锈病毒力频率及抗性基因检测

IF 0.9 4区农林科学

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-06-22 DOI: 10.17221/86/2019-cjgpb

A. Hanzalová, V. Dumalasová, Ondřej Zelba

引用次数: 7