Discrimination of tobacco cultivars using SCAR and RAPD markers

IF 1.2 4区农林科学 Q3 AGRONOMY

Czech Journal of Genetics and Plant Breeding Pub Date : 2020-07-20 DOI:10.17221/120/2019-cjgpb

Jiuzhen Sun, Junting Wang, Su Dongying, Yang Jinchu, Wang Erbin, Shixi Wu, Li Meng, Lin Ma

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引用次数: 2

Abstract

Tobacco genetic purity is crucial to maintain the quality of cigarette products in the tobacco industry. To reduce the difficulties in the discrimination of large number of tobacco cultivars in production practice, we developed a two-step identification strategy by using SCAR and RAPD markers. A total of 53 tobacco cultivars were examined in the study. Initially, all the selected cultivars were divided into four groups, each group consisted of seven to seventeen tobacco cultivars based on difference in phenotypes identified by the SCAR markers S4 and S8. Later, in each group, each tobacco cultivar was identified using RAPD fingerprinting by using one to four polymorphic primers, which were selected from 200 random primers. The results showed that all 53 tobacco cultivars could be effectively distinguished by using only two SCAR and seven RAPD markers. The two-step fingerprinting strategy could be used as a convenient and cost-effective tool to discriminate large numbers of tobacco cultivars for production planning in the tobacco industry.

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SCAR和RAPD标记在烟草品种鉴别中的应用

烟草基因纯度对烟草业保持卷烟产品质量至关重要。为了减少生产实践中对大量烟草品种的识别困难，我们建立了SCAR和RAPD两步识别策略。本研究共检测了53个烟草品种。首先，根据SCAR标记S4和S8所鉴定的表型差异，将所有选择的烟草品种分为4组，每组7 ~ 17个。随后，从200个随机引物中选取1 ~ 4个多态性引物，利用RAPD指纹图谱对每个烟草品种进行鉴定。结果表明，仅用2个SCAR和7个RAPD标记就能有效地对53个烟草品种进行鉴定。两步指纹识别技术可作为一种简便、经济的烟草品种鉴别方法，为烟草工业的生产规划提供依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Czech Journal of Genetics and Plant Breeding Agricultural and Biological Sciences-Plant Science

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Original scientific papers, critical reviews articles and short communications from the field of theoretical and applied plant genetics, plant biotechnology and plant breeding. Papers are published in English.