位于OsSUT3基因5'非翻译区一个31 bp的缺失影响基因表达和水稻花粉发育

IF 1.2 4区 农林科学 Q3 AGRONOMY
Chunlong Zhang, Qiuping Li, Hong Yang, Tuo Wang, Juan Li, J. Wen, S. Jin, Zhonglin Zhang, Lijuan Chen, Dandan Li
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引用次数: 2

摘要

OsSUT基因已被证明与水稻的各种生物过程有关。在本研究中,我们通过qRT-PCR和转基因研究确定了OsSUT3基因5’非翻译区(5’UTR)中31bp的插入与水稻穗中较高的OsSUT3表达之间的密切关系。这31bp的插入/缺失(indel)对水稻花粉发育和其他穗部性状,如花粉数量、花粉育性、播种率和粒长,都有统计学意义(P<0.01)。进化分析表明,在水稻驯化过程中,31bp插入的比例显著增加。因此,31bp Indel可以作为一种方便的分子标记,在水稻育种中筛选更多的花粉和更好的穗部性状。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A 31-bp indel localised in the 5' untranslated region of OsSUT3 affects the gene expression and rice (Oryza sativa L.) pollen development
OsSUT genes have been demonstrated to be relevant for diverse biological processes in rice. In this study, we identified the close relationship between a 31-bp insertion in a 5' untranslated region (5' UTR) of the OsSUT3 gene and higher OsSUT3 expression in rice panicles by qRT-PCR and transgenic research. Statistically significant results (P < 0.01) were found for this 31-bp insertions/deletions (indels) in the rice pollen development and other panicle traits, such as the pollen number, pollen fertility, seeding rate, and grain length. An evolution analysis showed that the proportion of the 31-bp insertion significantly increases in rice domestication. Therefore, the 31-bp Indel could be considered as a convenient molecular marker to screen more pollen and better panicle traits in rice breeding.
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来源期刊
Czech Journal of Genetics and Plant Breeding
Czech Journal of Genetics and Plant Breeding Agricultural and Biological Sciences-Plant Science
CiteScore
2.20
自引率
0.00%
发文量
25
审稿时长
>12 weeks
期刊介绍: Original scientific papers, critical reviews articles and short communications from the field of theoretical and applied plant genetics, plant biotechnology and plant breeding. Papers are published in English.
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