Differentiation最新文献_第6页

Ex vivo adult stem cell characterization from multiple muscles in ambulatory children with cerebral palsy during early development of contractures 在早期挛缩发展期间，可活动的脑瘫儿童多块肌肉的离体成体干细胞特征

IF 2.9 3区生物学

Differentiation Pub Date : 2023-09-01 DOI: 10.1016/j.diff.2023.06.003

M. Corvelyn , J. Meirlevede , J. Deschrevel , E. Huyghe , E. De Wachter , G. Gayan-Ramirez , M. Sampaolesi , A. Van Campenhout , K. Desloovere , D. Costamagna

{"title":"Ex vivo adult stem cell characterization from multiple muscles in ambulatory children with cerebral palsy during early development of contractures","authors":"M. Corvelyn , J. Meirlevede , J. Deschrevel , E. Huyghe , E. De Wachter , G. Gayan-Ramirez , M. Sampaolesi , A. Van Campenhout , K. Desloovere , D. Costamagna","doi":"10.1016/j.diff.2023.06.003","DOIUrl":"10.1016/j.diff.2023.06.003","url":null,"abstract":"<div><p>Cerebral palsy (CP) is one of the most common conditions leading to lifelong childhood physical disability. Literature reported previously altered muscle properties such as lower number of satellite cells (SCs), with altered fusion capacity. However, these observations highly vary among studies, possibly due to heterogeneity in patient population, lack of appropriate control data, methodology and different assessed muscle.</p><p>In this study we aimed to strengthen previous observations and to understand the heterogeneity of CP muscle pathology. Myogenic differentiation of SCs from the <em>Medial Gastrocnemius</em> (MG) muscle of patients with CP (n = 16, 3–9 years old) showed higher fusion capacity compared to age-matched typically developing children (TD, n = 13). Furthermore, we uniquely assessed cells of two different lower limb muscles and showed a decreased myogenic potency in cells from the <em>Semitendinosus</em> (ST) compared to the MG (TD: n = 3, CP: n = 6). Longitudinal assessments, one year after the first botulinum toxin treatment, showed slightly reduced SC representations and lower fusion capacity (n = 4). Finally, we proved the robustness of our data, by assessing in parallel the myogenic capacity of two samples from the same TD muscle.</p><p>In conclusion, these data confirmed previous findings of increased SC fusion capacity from MG muscle of young patients with CP compared to age-matched TD. Further elaboration is reported on potential factors contributing to heterogeneity, such as assessed muscle, CP progression and reliability of primary outcome parameters.</p></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10229587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Ectopic expression of HNF4α in Het1A cells induces an invasive phenotype HNF4α在Het1A细胞中异位表达诱导侵袭表型

IF 2.9 3区生物学

Differentiation Pub Date : 2023-08-23 DOI: 10.1016/j.diff.2023.08.003

Carmen Grimaldos Rodriguez , Ella F. Rimmer , Benjamin Colleypriest , David Tosh , Jonathan M.W. Slack , Ute Jungwirth

{"title":"Ectopic expression of HNF4α in Het1A cells induces an invasive phenotype","authors":"Carmen Grimaldos Rodriguez , Ella F. Rimmer , Benjamin Colleypriest , David Tosh , Jonathan M.W. Slack , Ute Jungwirth","doi":"10.1016/j.diff.2023.08.003","DOIUrl":"10.1016/j.diff.2023.08.003","url":null,"abstract":"<div><p>Barrett's oesophagus (BO) is a pathological condition in which the squamous epithelium of the distal oesophagus is replaced by an intestinal-like columnar epithelium originating from the gastric cardia. Several somatic mutations contribute to the intestinal-like metaplasia. Once these have occurred in a single cell, it will be unable to expand further unless the altered cell can colonise the surrounding squamous epithelium of the oesophagus. The mechanisms by which this happens are still unknown. Here we have established an <em>in vitro</em> system for examining the competitive behaviour of two epithelia. We find that when an oesophageal epithelium model (Het1A cells) is confronted by an intestinal epithelium model (Caco-2 cells), the intestinal cells expand into the oesophageal domain. In this case the boundary involves overgrowth by the Caco-2 cells and the formation of isolated colonies. Two key transcription factors, normally involved in intestinal development, HNF4α and CDX2, are both expressed in BO. We examined the competitive ability of Het1A cells stably expressing HNF4α or CDX2 and placed in confrontation with unmodified Het1A cells. The key result is that stable expression of HNF4α, but not CDX2, increased the ability of the cells to migrate and push into the unmodified Het1A domain. In this situation the boundary between the cell types is a sharp one, as is normally seen in BO. The experiments were conducted using a variety of extracellular substrates, which all tended to increase the cell migration compared to uncoated plastic. These data provide evidence that HNF4α expression could have a potential role in the competitive spread of BO into the oesophagus as HNF4α increases the ability of cells to invade into the adjacent stratified squamous epithelium, thus enabling a single mutant cell eventually to generate a macroscopic patch of metaplasia.</p></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10204864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corneal epithelial development and homeostasis 角膜上皮的发育和体内平衡

IF 2.9 3区生物学

Differentiation Pub Date : 2023-07-01 DOI: 10.1016/j.diff.2023.02.002

Shivalingappa K. Swamynathan , Sudha Swamynathan

引用次数: 0

Wiring the ocular surface: A focus on the comparative anatomy and molecular regulation of sensory innervation of the cornea 眼表连接:角膜感觉神经支配的比较解剖学和分子调控

IF 2.9 3区生物学

Differentiation Pub Date : 2023-07-01 DOI: 10.1016/j.diff.2023.01.002

Tyler Schwend

{"title":"Wiring the ocular surface: A focus on the comparative anatomy and molecular regulation of sensory innervation of the cornea","authors":"Tyler Schwend","doi":"10.1016/j.diff.2023.01.002","DOIUrl":"10.1016/j.diff.2023.01.002","url":null,"abstract":"<div><p>The cornea is richly innervated with sensory nerves that function to detect and clear harmful debris from the surface of the eye, promote growth and survival of the corneal epithelium and hasten wound healing following ocular disease or trauma. Given their importance to eye health, the neuroanatomy of the cornea has for many years been a source of intense investigation. Resultantly, complete nerve architecture maps exist for adult human and many animal models and these maps reveal few major differences across species. Interestingly, recent work has revealed considerable variation across species in how sensory nerves are acquired during developmental innervation of the cornea. Highlighting such species-distinct key differences, but also similarities, this review provides a full, comparative anatomy analysis of sensory innervation of the cornea for all species studied to date. Further, this article comprehensively describes the molecules that have been shown to guide and direct nerves toward, into and through developing corneal tissue as the final architectural pattern of the cornea’s neuroanatomy is established. Such knowledge is useful for researchers and clinicians seeking to better understand the anatomical and molecular basis of corneal nerve pathologies and to hasten neuro-regeneration following infection, trauma or surgery that damage the ocular surface and its corneal nerves.</p></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9790342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meibomian gland development: Where, when and how? 睑板腺发育:何时何地、如何发育?

IF 2.9 3区生物学

Differentiation Pub Date : 2023-07-01 DOI: 10.1016/j.diff.2023.04.005

Sudhir Verma , Isabel Y. Moreno , Morgan E. Trapp , Luis Ramirez , Tarsis F. Gesteira , Vivien J. Coulson-Thomas

{"title":"Meibomian gland development: Where, when and how?","authors":"Sudhir Verma , Isabel Y. Moreno , Morgan E. Trapp , Luis Ramirez , Tarsis F. Gesteira , Vivien J. Coulson-Thomas","doi":"10.1016/j.diff.2023.04.005","DOIUrl":"10.1016/j.diff.2023.04.005","url":null,"abstract":"<div><p>The Meibomian gland (MG) is an indispensable adnexal structure of eye that produces meibum, an important defensive component for maintaining ocular homeostasis. Normal development and maintenance of the MGs is required for ocular health since atrophic MGs and disturbances in composition and/or secretion of meibum result in major ocular pathologies, collectively termed as Meibomian gland dysfunction (MGD). Currently available therapies for MGD merely provide symptomatic relief and do not treat the underlying deficiency of the MGs. Hence, a thorough understanding of the timeline of MG development, maturation and aging is required for regenerative purposes along with signaling molecules & pathways controlling proper differentiation of MG lineage in mammalian eye. Understanding the factors that contribute to the development of MGs, developmental abnormalities of MGs, and changes in the quality & quantity of meibum with developing phases of MGs are essential for developing potential treatments for MGD. In this review, we compiled a timeline of events and the factors involved in the structural and functional development of MGs and the associated developmental defects of MGs during development, maturation and aging.</p></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9850100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Epigenetic regulation in the commitment of progenitor cells during retinal development and regeneration 视网膜发育和再生过程中祖细胞承诺的表观遗传调控

IF 2.9 3区生物学

Differentiation Pub Date : 2023-07-01 DOI: 10.1016/j.diff.2023.04.002

Wenjie Yin , Xiying Mao , Miao Xu, Mingkang Chen, Mengting Xue, Na Su, Songtao Yuan, Qinghuai Liu

{"title":"Epigenetic regulation in the commitment of progenitor cells during retinal development and regeneration","authors":"Wenjie Yin , Xiying Mao , Miao Xu, Mingkang Chen, Mengting Xue, Na Su, Songtao Yuan, Qinghuai Liu","doi":"10.1016/j.diff.2023.04.002","DOIUrl":"10.1016/j.diff.2023.04.002","url":null,"abstract":"<div><p>Retinal development is initiated by multipotent retinal progenitor cells, which undergo several rounds of cell divisions and subsequently terminal differentiation. Retinal regeneration is usually considered as the recapitulation of retinal development, which share common mechanisms underlying the cell cycle re-entry of adult retinal stem cells and the differentiation of retinal neurons. However, how proliferative retinal progenitor cells perform a precise transition to postmitotic retinal cell types during the process of development and regeneration remains elusive. It is proposed that both the intrinsic and extrinsic programming are involved in the transcriptional regulation of the spatio-temporal fate commitment. Epigenetic modifications and the regulatory mechanisms at both DNA and chromatin levels are also postulated to play an important role in the timing of differentiation of specific retinal cells. In the present review, we have summarized recent knowledge of epigenetic regulation that underlies the commitment of retinal progenitor cells in the settings of retinal development and regeneration.</p></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10166785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Ocular development: A view from the front to the back of the eye 眼部发育：从眼睛前部到后部的视图

IF 2.9 3区生物学

Differentiation Pub Date : 2023-07-01 DOI: 10.1016/j.diff.2023.06.004

Anna La Torre, Peter Lwigale

引用次数: 0

Characterizing the lens regeneration process in Pleurodeles waltl 描述了胸膜菌的晶状体再生过程。

IF 2.9 3区生物学

Differentiation Pub Date : 2023-07-01 DOI: 10.1016/j.diff.2023.02.003

Georgios Tsissios , Gabriella Theodoroudis-Rapp , Weihao Chen , Anthony Sallese , Byran Smucker , Lake Ernst , Junfan Chen , Yiqi Xu , Sophia Ratvasky , Hui Wang , Katia Del Rio-Tsonis

{"title":"Characterizing the lens regeneration process in Pleurodeles waltl","authors":"Georgios Tsissios , Gabriella Theodoroudis-Rapp , Weihao Chen , Anthony Sallese , Byran Smucker , Lake Ernst , Junfan Chen , Yiqi Xu , Sophia Ratvasky , Hui Wang , Katia Del Rio-Tsonis","doi":"10.1016/j.diff.2023.02.003","DOIUrl":"10.1016/j.diff.2023.02.003","url":null,"abstract":"<div><h3>Background</h3><p>Aging and regeneration are heavily linked processes. While it is generally accepted that regenerative capacity declines with age, some vertebrates, such as newts, can bypass the deleterious effects of aging and successfully regenerate a lens throughout their lifetime.</p></div><div><h3>Results</h3><p>Here, we used Spectral-Domain Optical Coherence Tomography (SD-OCT) to monitor the lens regeneration process of larvae, juvenile, and adult newts. While all three life stages were able to regenerate a lens through transdifferentiation of the dorsal iris pigment epithelial cells (iPECs), an age-related change in the kinetics of the regeneration process was observed. Consistent with these findings, iPECs from older animals exhibited a delay in cell cycle re-entry. Furthermore, it was observed that clearance of the extracellular matrix (ECM) was delayed in older organisms.</p></div><div><h3>Conclusions</h3><p>Collectively, our results suggest that although lens regeneration capacity does not decline throughout the lifespan of newts, the intrinsic and extrinsic cellular changes associated with aging alter the kinetics of this process. By understanding how these changes affect lens regeneration in newts, we can gain important insights for restoring the age-related regeneration decline observed in most vertebrates.</p></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10573632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Spheroid formation and luteinization of granulosa cells of felids in a long-term 3D culture 在长期三维培养中，田体颗粒细胞的球状形成和黄体化

IF 2.9 3区生物学

Differentiation Pub Date : 2023-05-01 DOI: 10.1016/j.diff.2023.03.002

Michał M. Hryciuk , Filip Schröter , Luise Hennicke , Beate C. Braun

{"title":"Spheroid formation and luteinization of granulosa cells of felids in a long-term 3D culture","authors":"Michał M. Hryciuk , Filip Schröter , Luise Hennicke , Beate C. Braun","doi":"10.1016/j.diff.2023.03.002","DOIUrl":"10.1016/j.diff.2023.03.002","url":null,"abstract":"<div><p>In the present study, granulosa cells (GCs) from domestic cats and Persian leopard were cultured and characterized from selected days. The culture period was divided into two phases: maintenance, which lasted for 7 days, and luteinization, which followed for up to 11 days. Luteinization was performed on ultra-low attachment plates, supporting the formation of spheroids in a medium supplemented with insulin, forskolin, and luteinizing hormone (LH). GCs of domestic cat produced estradiol (E2) and progesterone (P4) during the maintenance phase. The gene expressions of some proteins involved in steroidogenesis were stable (<em>STAR</em>, <em>HSD3B1</em>) or decreased over time (<em>CYP11A1</em>, <em>HSD17B1</em>, <em>CYP17A1</em>, and <em>CYP19A1</em>), which was similar to the expressions of gonatropin receptors (<em>LHCGR</em> and <em>FSHR</em>). During the luteinization phase, P4 concentration significantly increased (<em>P</em> < 0.05), and E2, in contrast to the proliferation phase, was below detection range. The expression of genes of proteins involved in steroidogenesis (<em>STAR</em>, <em>CYP11A1</em>, <em>HSD3B1</em>, <em>HSD17B1</em>, <em>CYP17A1</em>, and <em>CYP19A1</em>) and of gonadotropin receptors (<em>LHCGR</em> and <em>FSHR</em>) significantly increased during the luteinization period, but some expressions exhibited a decrease at the end of the phase (<em>LHCGR</em>, <em>FSHR</em>, <em>HSD17B1</em>, <em>CYP19A1</em>). The morphology of the luteinized GCs of domestic cat resembled large luteal cells and had numerous vacuole-like structures. Also, the GCs of Persian leopard underwent luteinization, shown by increasing P4 production and <em>HSD3B1</em> expression. This study confirms that GCs from felids can be luteinized in a 3D spheroid system which can be a basis for further studies on luteal cell function of felids. Additionally, we could show that the domestic cat can serve as a model species for establishing cell culture methods which can be transferred to other felids.</p></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9681931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reprogramming of trunk neural crest to a cranial crest-like identity alters their transcriptome and developmental potential 主干神经嵴重编程为颅嵴样身份改变了它们的转录组和发育潜力

IF 2.9 3区生物学

Differentiation Pub Date : 2023-05-01 DOI: 10.1016/j.diff.2023.04.001

Sierra S. Marable, Marianne E. Bronner

{"title":"Reprogramming of trunk neural crest to a cranial crest-like identity alters their transcriptome and developmental potential","authors":"Sierra S. Marable, Marianne E. Bronner","doi":"10.1016/j.diff.2023.04.001","DOIUrl":"10.1016/j.diff.2023.04.001","url":null,"abstract":"<div><p>Neural crest cells along the body axis of avian embryos differ in their developmental potential, such that the cranial neural crest forms cartilage and bone whereas the trunk neural crest is unable to do so. Previous studies have identified a cranial crest-specific subcircuit that can imbue the trunk neural crest with the ability to form cartilage after grafting to the head. Here, we examine transcriptional and cell fate changes that accompany this reprogramming. First, we examined whether reprogrammed trunk neural crest maintain the ability to form cartilage in their endogenous environment in the absence of cues from the head. The results show that some reprogrammed cells contribute to normal trunk neural crest derivatives, whereas others migrate ectopically to the forming vertebrae and express cartilage markers, thus mimicking heterotypically transplanted cranial crest cells. We find that reprogrammed trunk neural crest upregulated more than 3000 genes in common with cranial neural crest, including numerous transcriptional regulators. In contrast, many trunk neural crest genes are downregulated. Together, our findings show that reprogramming trunk neural crest with cranial crest subcircuit genes alters their gene regulatory program and developmental potential to be more cranial crest-like.</p></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":null,"pages":null},"PeriodicalIF":2.9,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10330191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9759856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0