Differentiation最新文献

Mettl3 is required for germline function during Drosophila spermatogenesis 在果蝇精子发生过程中，Mettl3是种系功能所必需的

IF 2.2 3区生物学

Differentiation Pub Date : 2025-07-13 DOI: 10.1016/j.diff.2025.100895

Alannah Morse, Hailey Kaba, Corinne Leighty, Emily MacLean, Rosemarie Mirabella, Mary Reinaker, Rohan Harris, Jazmyn Moodie, Antonio Rockwell

{"title":"Mettl3 is required for germline function during Drosophila spermatogenesis","authors":"Alannah Morse, Hailey Kaba, Corinne Leighty, Emily MacLean, Rosemarie Mirabella, Mary Reinaker, Rohan Harris, Jazmyn Moodie, Antonio Rockwell","doi":"10.1016/j.diff.2025.100895","DOIUrl":"10.1016/j.diff.2025.100895","url":null,"abstract":"<div><div>The m<sup>6</sup>A modification is responsible for regulating several aspects of RNA metabolism. The enzyme that catalyzes this modification Mettl3, is highly conserved and required for numerous biological processes such as spermatogenesis. Here we examine the role of Mettl3 in germline function during <em>Drosophila</em> spermatogenesis. We find that depletion of Mettl3 in the germline results in errors in late-stage spermatogenesis, the process known as spermiogenesis. In germline knockdowns, actin cones bound to spermatids fail to remain tightly packed during the individualization process. Issues with actin cone assembly appear to disrupt progression through spermiogenesis resulting in waste bag deficiency, an indicator of abnormal spermatid individualization. These errors result in little to no sperm in seminal vesicles culminating in reduced fertility in germline knockdowns. Furthermore, our findings suggest <em>Hsp60B</em> is misregulated in knockdowns, which potentially explains at least some observed phenotypes. Collectively, the data presented in this investigation suggests Mettl3 has a prominent role in regulating spermatid differentiation during <em>Drosophila</em> spermatogenesis.</div></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":"145 ","pages":"Article 100895"},"PeriodicalIF":2.2,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bdnf and Ntrk2 functions promote but are not essential for spinal cord myelination in larval zebrafish Bdnf和Ntrk2的功能促进了斑马鱼幼体脊髓髓鞘形成，但不是必需的

IF 2.2 3区生物学

Differentiation Pub Date : 2025-07-12 DOI: 10.1016/j.diff.2025.100896

Kristen Russell , Christina A. Kearns , Macie B. Walker , Christopher S. Knoeckel , Angeles B. Ribera , Caleb A. Doll , Bruce Appel

{"title":"Bdnf and Ntrk2 functions promote but are not essential for spinal cord myelination in larval zebrafish","authors":"Kristen Russell , Christina A. Kearns , Macie B. Walker , Christopher S. Knoeckel , Angeles B. Ribera , Caleb A. Doll , Bruce Appel","doi":"10.1016/j.diff.2025.100896","DOIUrl":"10.1016/j.diff.2025.100896","url":null,"abstract":"<div><div>Myelin, a specialized membrane produced by oligodendroglial cells in the central nervous system, wraps axons to enhance conduction velocity and maintain axon health. Not all axons are myelinated, and not all myelinated axons are uniformly wrapped along their lengths. Several lines of evidence indicate that neuronal activity can influence myelination, however, the cellular and molecular mechanisms that mediate communication between axons and oligodendrocytes remain poorly understood. Prior research showed that the neurotrophic growth factor Bdnf and its receptor Ntrk2 promote myelination in rodents, raising the possibility that Bdnf and Ntrk2 convey myelin-promoting signals from neurons to oligodendrocytes. We explored this possibility using a combination of gene expression analyses, gene function tests, and myelin sheath formation assays in zebrafish larvae. Altogether, our data indicate that, although not essential for myelination, Bdnf-Ntrk2 signaling contributes to the timely formation of myelin in the developing zebrafish spinal cord.</div></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":"145 ","pages":"Article 100896"},"PeriodicalIF":2.2,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “The mechanism of EGF in promoting skeletal muscle post-injury regeneration” [Differentiation 143 (2025) 100862] “EGF促进骨骼肌损伤后再生的机制”的更正[分化143 (2025)100862]

IF 2.2 3区生物学

Differentiation Pub Date : 2025-07-01 DOI: 10.1016/j.diff.2025.100885

Huaixin Teng , Yongze Liu , Ruotong Hao , Lu Zhang , Xiaoyu Zhang , Shufeng Li , Shuang Li , Huili Tong

引用次数: 0

One Wnt to lead them all: a Wnt1 primer 一个Wnt可以引领所有Wnt：一个Wnt1引物

IF 2.2 3区生物学

Differentiation Pub Date : 2025-06-24 DOI: 10.1016/j.diff.2025.100884

Arne C. Lekven, Sarah Empie , Richard Saoud

引用次数: 0

Characterization of Pumilio gene expression during early neural crest development 神经嵴发育早期Pumilio基因表达的研究

IF 2.2 3区生物学

Differentiation Pub Date : 2025-06-23 DOI: 10.1016/j.diff.2025.100883

Mariann Guzman-Espinoza , Helen M. Vander Wende , Jessica L. Pacheco , Alejandra Olano Roldán , Erica J. Hutchins

{"title":"Characterization of Pumilio gene expression during early neural crest development","authors":"Mariann Guzman-Espinoza , Helen M. Vander Wende , Jessica L. Pacheco , Alejandra Olano Roldán , Erica J. Hutchins","doi":"10.1016/j.diff.2025.100883","DOIUrl":"10.1016/j.diff.2025.100883","url":null,"abstract":"<div><div>Neural crest cells are multipotent cells present in vertebrate embryos that give rise to a wide array of cell types and tissues. A growing number of studies have identified post-transcriptional regulatory events that are essential for multiple stages of neural crest development, though a thorough characterization of the post-transcriptional regulators controlling these events is currently lacking. From single cell RNA-sequencing data, we identified members of the Pumilio family of RNA-binding proteins, PUM1 and PUM2, as candidate post-transcriptional regulators of neural crest development. Using hybridization chain reaction (HCR) in avian embryos (<em>Gallus gallus</em>), we characterized the spatiotemporal expression of <em>Pumilio</em> family mRNAs during early stages of cranial neural crest development. We show that <em>Pum1</em> and <em>Pum2</em>, though expressed throughout the three germ layers, were enriched in ectodermally-derived tissues, and following neurulation, <em>Pum1</em> and <em>Pum2</em> show distinct expression patterns. We observed that <em>Pum1</em> displayed a more uniform expression throughout the neural tube and neural crest during neural crest specification and the epithelial-mesenchymal transition (EMT). In contrast, <em>Pum2</em> was enriched in neural crest cells poised to undergo EMT. We thus hypothesize that PUM1 and PUM2, often speculated to be functionally redundant, may play distinct roles at key steps of neural crest development.</div></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":"144 ","pages":"Article 100883"},"PeriodicalIF":2.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144489521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developmental biology in Chile: an open window to Latin America and the world 智利的发育生物学：向拉丁美洲和世界打开的一扇窗

IF 2.2 3区生物学

Differentiation Pub Date : 2025-06-16 DOI: 10.1016/j.diff.2025.100882

Miguel L. Concha , Marcia Gaete , Sylvain Marcellini , Verónica Palma , Marcela Torrejón , Constanza Vásquez-Doorman

{"title":"Developmental biology in Chile: an open window to Latin America and the world","authors":"Miguel L. Concha , Marcia Gaete , Sylvain Marcellini , Verónica Palma , Marcela Torrejón , Constanza Vásquez-Doorman","doi":"10.1016/j.diff.2025.100882","DOIUrl":"10.1016/j.diff.2025.100882","url":null,"abstract":"<div><div>The field of developmental biology has a relatively short history in Chile. It began with the pioneering work of Luis Izquierdo in the 1950s, when he challenged the prevailing views on embryology and comparative anatomy. Since then, the developmental biology community has grown considerably, broadening its vision and perspectives, as well as its international presence. Within the Latin American context, Chile has played a leading role in promoting the discipline and training a new generation of developmental biologists. In 2024, the country hosted several impactful regional events including a hands-on course, an international conference, a global lecture series, and the inaugural congress of the Chilean Society for Developmental Biology (SBDCh). The pivotal role played by the European Molecular Biology Organisation (EMBO), the United Nations University Programme for Biotechnology in Latin America and the Caribbean (UNU-BIOLAC), and the International Society of Differentiation (ISD) in supporting Chile's leadership in the field is also worth highlighting. These organisations enabled the participation of young Latin American researchers by providing travel grants and support. This is particularly relevant in today's changing world, where global integration and collaboration are fundamental to the effective promotion of developmental biology, especially among younger generations.</div></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":"144 ","pages":"Article 100882"},"PeriodicalIF":2.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enteric neural crest development in Astyanax mexicanus surface fish and cavefish 面鱼和穴居鱼肠道神经嵴发育的研究

IF 2.2 3区生物学

Differentiation Pub Date : 2025-06-13 DOI: 10.1016/j.diff.2025.100881

Pavani Ponnimbaduge Perera , Kaitlyn Webster , Misty R. Riddle

{"title":"Enteric neural crest development in Astyanax mexicanus surface fish and cavefish","authors":"Pavani Ponnimbaduge Perera , Kaitlyn Webster , Misty R. Riddle","doi":"10.1016/j.diff.2025.100881","DOIUrl":"10.1016/j.diff.2025.100881","url":null,"abstract":"<div><div>The enteric nervous system (ENS) regulates gastrointestinal (GI) functions such as secretion, blood flow, and motility, yet how its structure and function evolve with dietary adaptations remains unclear. <em>Astyanax mexicanus</em>, a teleost fish with surface and cave morphotypes, offers a model to explore these changes; cavefish exhibit altered GI motility and transit that may help them adapt to their unique diet. We compared early ENS development in surface fish and cavefish, tracking enteric neural crest cell (ENCC) migration and differentiation using <em>phox2bb</em> and HuC/D markers. We found that ENCCs reach the gut by 36 hours post-fertilization (hpf) in both morphotypes but migrate and differentiate more rapidly along the gut in cavefish. To explore the genetic basis of this difference, we used available genomic datasets to compare the predicted peptide sequences of genes important for ENS development in other species and identified mutations that could impact protein function, for example in the endothelin signaling genes important for ENCC migration and differentiation. We specifically examined the expression of <em>endothelin-3 (edn3)</em> and <em>endothelin receptor-b a (ednrba)</em> during ENCC migration and found that the localization of <em>edn3</em>, but not <em>ednrba</em>, is consistent with a potential role in ENS development. Overall, our findings establish <em>A. mexicanus</em> as a model for studying evolution of ENS development.</div></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":"144 ","pages":"Article 100881"},"PeriodicalIF":2.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Origin stories of neural crest roles in craniofacial development: A tale of the meninges 颅面发育中神经嵴作用的起源故事：脑膜的故事

IF 2.2 3区生物学

Differentiation Pub Date : 2025-06-05 DOI: 10.1016/j.diff.2025.100880

Diana Rigueur , Julia C. Boughner

{"title":"Origin stories of neural crest roles in craniofacial development: A tale of the meninges","authors":"Diana Rigueur , Julia C. Boughner","doi":"10.1016/j.diff.2025.100880","DOIUrl":"10.1016/j.diff.2025.100880","url":null,"abstract":"<div><div>As developmental biologists, we tell origin stories to explain differences in vertebrate craniofacial form and complexity. Some of these differences are linked to diseases, notably of patients born with congenital malformations, many of them syndromic. These defective processes impede proper neural crest cell migration, differentiation, and patterning of bones, cartilage, joints, muscle and other connective tissues, including of the head. For example, a rare form of holoprosencephaly called cyclopia is a particular quandary in its diverse presentation. This syndromic congenital disease includes lost brain frontal lobe septation and development of a single eye. Subsequent craniofacial midline defects include frontal suture synostosis, absent nose and medial maxilla, and a restructuring of facial bones to accommodate one eye. The meningeal tissues in contact with both the underlying brain and the overlying calvarial bone are derived at least in part from neural crest cells. This Perspectives piece re-examines communication between the neural crest cell-brain axis, explores the direct involvement of the meninges in this communication process, and proposes an expanded origin story about the etiology of craniofacial patterning and disease.</div></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":"144 ","pages":"Article 100880"},"PeriodicalIF":2.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteins and peptides responsible for bone remodeling 负责骨重塑的蛋白质和多肽

IF 2.2 3区生物学

Differentiation Pub Date : 2025-05-29 DOI: 10.1016/j.diff.2025.100872

Jorge Alejandro Barbosa-Nuñez , José Nabor Haro-González , Eristeo García-Márquez , Hugo Espinosa-Andrews , Eduardo Padilla-Camberos , Sara Elisa Herrera-Rodríguez

{"title":"Proteins and peptides responsible for bone remodeling","authors":"Jorge Alejandro Barbosa-Nuñez , José Nabor Haro-González , Eristeo García-Márquez , Hugo Espinosa-Andrews , Eduardo Padilla-Camberos , Sara Elisa Herrera-Rodríguez","doi":"10.1016/j.diff.2025.100872","DOIUrl":"10.1016/j.diff.2025.100872","url":null,"abstract":"<div><div>Bones are living tissues that are periodically renewed through the bone remodeling process. It starts when monocyte-macrophage-type cells begin to proliferate and recruit into the bone tissue due to the action of various proteins. The proteins are generated by the body in response to a lack of calcium in the blood or a damaged bone tissue. Then, other chemical signals promote the differentiation of monocyte-macrophage-type cells into osteoclasts. Finally, osteoclasts carry out a process known as bone resorption in which they degrade bone tissue. After, bone resorption a process known as osteogenesis takes place. In osteogenesis, pluripotent mesenchymal stem cells proliferate and recruit in the bone damaged by bone resorption in response to the action of different compounds. Then, pluripotent mesenchymal stem cells differentiate into osteoblasts due to the effect of specific proteins. Finally, osteoblasts generate new bone tissue, completing the bone remodeling process. This review aims to summarize and provide recent findings of the proteins and chemical signals involved in osteoclast and osteoblast proliferation, recruitment and maturation during the bone resorption and osteogenesis processes. The information found about these molecules could help to better understand all the complex mechanisms that directly or indirectly influence the bone remodeling process. In this sense, the mechanisms of different bone disorders and diseases could be elucidated in a better way, leading to the generation of efficient and specific treatments for each of them in the future.</div></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":"144 ","pages":"Article 100872"},"PeriodicalIF":2.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myogenic and adipogenic potential of porcine muscle satellite cells isolated by flow cytometry 用流式细胞术分离猪肌肉卫星细胞的成肌和成脂潜能

IF 2.2 3区生物学

Differentiation Pub Date : 2025-05-27 DOI: 10.1016/j.diff.2025.100871

A. Vincent , K. Stange , I. Louveau , M. Röntgen , F. Dessauge

{"title":"Myogenic and adipogenic potential of porcine muscle satellite cells isolated by flow cytometry","authors":"A. Vincent , K. Stange , I. Louveau , M. Röntgen , F. Dessauge","doi":"10.1016/j.diff.2025.100871","DOIUrl":"10.1016/j.diff.2025.100871","url":null,"abstract":"<div><div>Skeletal muscle mesenchymal stromal cells (MSCs) are resident cells in the stromal, interstitial or perivascular areas, with satellite cells (SCs) acting as essential precursors for muscle growth and regeneration. This study firstly aimed to phenotype freshly isolated SCs using cell surface markers and gene expression and to assess their ability to differentiate in myogenic or adipogenic conditions. Then, refined SCs populations sorted according to the myogenic CD56 cell surface marker were characterized. SCs were isolated from the l<em>ongissimus</em> muscle of 5-7-day-old piglets and analyzed using flow cytometry. The hematopoietic CD45 + cells represented 20 % of the total isolated cell population. The myogenic CD29 and CD56 positive cell populations were the most abundant (80 % and 50 % respectively). Mesenchymal CD90 positive cells were also highly present (34 %) while the proportions of fibro-adipogenic CD140a and CD34-positive cells were low (<3 %). In this study, we showed that total isolated muscle-derived SCs were able to differentiate into myotubes in both myogenic and adipogenic media. Transcriptional profiles were similar, except for PPARγ, PGC1α, and Myosin Heavy Chain 2B witch present a higher induction during differentiation in the adipogenic medium. Interestingly, non-hematopoietic CD45<sup>−</sup>sorted cells further separated in CD45-/CD56+ and also CD45-/CD56-sub-populations mostly formed myotubes under both conditions, with CD56<sup>−</sup>cells showing potential and gene expression profile of myoblasts. Mature adipocytes were found in the CD45-/CD56+ group after differentiation in adipogenic medium. This study increases knowledge on myogenic cell surface marker and underscores the complexity and heterogeneity of muscle SCs.</div></div>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":"144 ","pages":"Article 100871"},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144169608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0