Clinica chimica acta; international journal of clinical chemistry最新文献

{"title":"CCR5 Δ32 minorallele is associated with susceptibility to SARS-CoV-2 infection and death: An epidemiological investigation.","authors":"Aditya K Panda, Archana Padhi, B Anjan Kumar Prusty","doi":"10.1016/j.cca.2020.07.012","DOIUrl":"https://doi.org/10.1016/j.cca.2020.07.012","url":null,"abstract":"","PeriodicalId":504940,"journal":{"name":"Clinica chimica acta; international journal of clinical chemistry","volume":" ","pages":"60-61"},"PeriodicalIF":5.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cca.2020.07.012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38144609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Further improvement of the quality of tube transportation system is needed to prevent 'seasonal' pseudohyperkalaemia.","authors":"Dominika Szoke, Simone Caruso, Sara Pasqualetti, Mauro Panteghini","doi":"10.1016/j.cca.2020.08.021","DOIUrl":"https://doi.org/10.1016/j.cca.2020.08.021","url":null,"abstract":"","PeriodicalId":504940,"journal":{"name":"Clinica chimica acta; international journal of clinical chemistry","volume":" ","pages":"644-646"},"PeriodicalIF":5.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cca.2020.08.021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38267423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persistently elevated hCG in a patient with no clear evidence of pregnancy.","authors":"Caroline Miller,&nbsp;Miriam McQuade,&nbsp;Julia MacCallum,&nbsp;Y Victoria Zhang","doi":"10.1016/j.cca.2020.09.004","DOIUrl":"https://doi.org/10.1016/j.cca.2020.09.004","url":null,"abstract":"<p><strong>Background: </strong>We describe a case of a female patient with a complex medical history, including squamous cell carcinoma of the tongue, and persistent elevation in serum hCG and no confirmed intrauterine or extrauterine pregnancy.</p><p><strong>Methods: </strong>The clinical chemistry laboratory was called upon to help clarify the situation and reach a diagnosis.</p><p><strong>Results: </strong>Multiple potential sources of elevated hCG were considered and excluded.</p><p><strong>Conclusions: </strong>This case shows a rare example of paraneoplastic hCG expression and highlights the role of the clinical chemistry laboratory in resolving diagnostic dilemmas and interpreting laboratory results in patients with multiple medical comorbidities.</p>","PeriodicalId":504940,"journal":{"name":"Clinica chimica acta; international journal of clinical chemistry","volume":" ","pages":"703-706"},"PeriodicalIF":5.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cca.2020.09.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38366077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study.","authors":"Joëlle C Schutten,&nbsp;Adrian Post,&nbsp;Margriet van der Meer,&nbsp;Jan IJmker,&nbsp;Frans Goorman,&nbsp;Richard M Danel,&nbsp;Marc G Vervloet,&nbsp;Martin H de Borst,&nbsp;Daan J Touw,&nbsp;Stephan J L Bakker","doi":"10.1016/j.cca.2020.09.007","DOIUrl":"https://doi.org/10.1016/j.cca.2020.09.007","url":null,"abstract":"<p><strong>Background: </strong>Direct methods for the assessment of intra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. An alternative, less labor intensive method is indirect calculation of intra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study.</p><p><strong>Methods: </strong>dIEM and iIEM were measured using a validated inductively coupled plasma mass spectrometry (ICP-MS) method in 1669 individuals from the second screening from the LifeLines Cohort Study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excretion.</p><p><strong>Results: </strong>Mean dIEM and iIEM were 0.20 ± 0.04 mmol/10¹² cells and 0.25 ± 0.04 mmol/10¹² cells, respectively. We found a strong correlation between dIEM and iIEM (r = 0.75). Passing-Bablok regression analyses showed an intercept of 0.015 (95% CI: 0.005; 0.023) and a slope of 1.157 (95% CI: 1.109; 1.210). In linear regression analyses, plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magnesium.</p><p><strong>Conclusions: </strong>We observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable alternative for the labor intensive dIEM method.</p>","PeriodicalId":504940,"journal":{"name":"Clinica chimica acta; international journal of clinical chemistry","volume":" ","pages":"772-780"},"PeriodicalIF":5.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cca.2020.09.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38372499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibody profiles comprising anti phosphatidylserine/prothrombin differently affect thrombin generation and protein C resistance in antiphospholipid antibody carriers.","authors":"Chunyan Cheng,&nbsp;Elena Pontara,&nbsp;Marta Tonello,&nbsp;Maria Grazia Cattini,&nbsp;Elisa Bison,&nbsp;Gentian Denas,&nbsp;Vittorio Pengo","doi":"10.1016/j.cca.2020.09.025","DOIUrl":"https://doi.org/10.1016/j.cca.2020.09.025","url":null,"abstract":"<p><p>Anti phosphatidylserine/prothrombin antibodies (aPS/PT) are currently not included in the laboratory work-up of antiphospholipid symdrome (APS). However, several studies indicate that aPS/PT confer additional risk for thromboembolic events when added to classical antiphospholipid (aPL) antibody panel. We aimed to study thrombin generation (TG), a test that describes hyper or hypo-coagulability, in a cohort of antiphospholipid antibody (aPL) carriers with or without aPS/PT. As oral anticoagulants interfere with TG, we performed the study in carriers of aPL antibodies not on oral anticoagulants treatment. TG in tissue factor-triggered platelet-poor plasma and its inhibition by thrombomodulin was measured with a calibrated automated thrombogram method. Data are expressed as minutes (Interquartile Range). Of 55 aPL carriers, 37 were positive and 18 were negative for aPS/PT. Lag Time 5.4 min (4.1; 7.3) vs 3.4 min (3.0;4.5) is significant longer (p < 0.0001) and time to peak 9.6 min (8.1;11) vs 7.7 min (6.8;8.8) is significantly delayed (p = 0.0011) in aPS/PT positive as compared to aPS/PT negative carriers. Endogenous Thrombin Potential (ETP), peak thrombin formation and the velocity index are lower in aPS/PT positive carriers but did not reach statistical significance. Inhibition of ETP by thrombomodulin was significantly lower (protein C resistance) in aPS/PT positive vs aPS/PT negative group (22.8%±11.5 vs 34.9%±20.4, p = 0.01). In conclusion, aPS/PT positive carriers show an anticoagulant effect in TG while they exert a procoagulant effect in response to thrombomodulin-activated protein C.</p>","PeriodicalId":504940,"journal":{"name":"Clinica chimica acta; international journal of clinical chemistry","volume":" ","pages":"796-801"},"PeriodicalIF":5.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cca.2020.09.025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38422953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the Martín-Sánchez indices for distinguishing beta thalassemia trait from iron deficiency anemia.","authors":"Johannes J M L Hoffmann,&nbsp;Eloísa Urrechaga","doi":"10.1016/j.cca.2020.08.028","DOIUrl":"https://doi.org/10.1016/j.cca.2020.08.028","url":null,"abstract":"","PeriodicalId":504940,"journal":{"name":"Clinica chimica acta; international journal of clinical chemistry","volume":" ","pages":"617-618"},"PeriodicalIF":5.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cca.2020.08.028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38320419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suppression tumorigenicity 2 (ST2) turbidimetric immunoassay compared to enzyme-linked immunosorbent assay in predicting survival in heart failure patients with reduced ejection fraction.","authors":"Lindsey Aurora,&nbsp;Edward Peterson,&nbsp;Hongsheng Gui,&nbsp;Nicole Zeld,&nbsp;James McCord,&nbsp;Yigal Pinto,&nbsp;Bernard Cook,&nbsp;Hani N Sabbah,&nbsp;L Keoki Williams,&nbsp;James Snider,&nbsp;David E Lanfear","doi":"10.1016/j.cca.2020.08.039","DOIUrl":"https://doi.org/10.1016/j.cca.2020.08.039","url":null,"abstract":"<p><strong>Background: </strong>Suppressor of tumorigenicity 2 (ST2) is a powerful marker of prognosis and treatment response in heart failure (HF), however, it is an enzyme-linked immunosorbent assay (ELISA) which may be cumbersome and costly. A turbidimetric immunoassay (TIA) that can run on common chemistry analyzers could overcome this. We studied a novel TIA for ST2, comparing it to commercial ST2 (ELISA).</p><p><strong>Methods: </strong>Patients age ≥ 18 years meeting Framingham definition for HF were enrolled in a prospective registry (Oct 2007 - March 2015) at Henry Ford Hospital and donated blood samples. Participants with reduced ejection fraction (<50%) and available plasma samples were included and valid ST2 measurements were obtained on the same sample using both TIA and ELISA (N = 721). The primary endpoint was all cause death. Correlation between the methods was quantified. The association with survival was tested using unadjusted and adjusted (for MAGGIC score and NTproBNP) Cox models and comparing the Area Under the Curve (AUC).</p><p><strong>Results: </strong>The inter-assay Spearman correlation coefficient was 0.77. Nonparametric regression showed no significant proportional difference (slope = 0.97) and a very small systematic difference (3.2 ng/mL). In univariate analyses, both TIA and ELISA ST2 were significant associates of survival with similar effect sizes (HR 4.46 and 3.50, respectively, both p < 0.001). In models adjusted for MAGGIC score, both ST2 remained significant in Cox models and incrementally improved AUC vs. MAGGIC alone (MAGGIC AUC = 0.757; TIA + MAGGIC AUC = 0.786, p = 0.025; ELISA + MAGGIC AUC = 0.793, p = 0.033). In models with both MAGGIC and NTproBNP included, both ST2 still remained significant but did not improve AUC.</p><p><strong>Conclusions: </strong>A novel TIA method for ST2 quantification correlates highly with ELISA and offers similarly powerful risk-stratification.</p>","PeriodicalId":504940,"journal":{"name":"Clinica chimica acta; international journal of clinical chemistry","volume":" ","pages":"767-771"},"PeriodicalIF":5.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cca.2020.08.039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38377830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical validation of the droplet digital PCR assay for diagnosis of spinal muscular atrophy.","authors":"Sunggyun Park,&nbsp;Hyeonah Lee,&nbsp;Saeam Shin,&nbsp;Seung-Tae Lee,&nbsp;Kyung-A Lee,&nbsp;Jong Rak Choi","doi":"10.1016/j.cca.2020.09.024","DOIUrl":"https://doi.org/10.1016/j.cca.2020.09.024","url":null,"abstract":"<p><strong>Background: </strong>Spinal muscular atrophy (SMA) is a progressive motor neuron disease caused by homozygote loss of exon 7 on the survival motor neuron 1 (SMN1) gene. The severity of the SMA phenotype is influenced by copies of SMN2, a gene that is highly homologous with SMN1.</p><p><strong>Methods: </strong>We validated analytical performance of droplet digital polymerase chain reaction (ddPCR) for detection of copy number variation of SMN1 and SMN2 genes for diagnosis of SMA using clinical samples. For accuracy performance evaluation, ddPCR results were compared with those of multiplex ligation-dependent probe amplification (MLPA) as a reference standard. Copy numbers of SMN1/SMN2 exon 7 from 200 clinical samples were concordant between ddPCR and MLPA.</p><p><strong>Results: </strong>For all samples, the copy number of SMN1/SMN2 exon 7 was concordant between MLPA and ddPCR. The ddPCR also showed acceptable degrees of repeatability and total imprecision.</p><p><strong>Conclusion: </strong>Therefore, ddPCR is expected to be useful for SMA diagnosis and to predict phenotypic severity of SMA patients by determining the copy number of SMN2 in clinical laboratories.</p>","PeriodicalId":504940,"journal":{"name":"Clinica chimica acta; international journal of clinical chemistry","volume":" ","pages":"787-789"},"PeriodicalIF":5.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cca.2020.09.024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38405201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into pediatric multi-system inflammatory syndrome and COVID-19.","authors":"David Bar-Or,&nbsp;Leonard T Rael,&nbsp;Edward N Brody","doi":"10.1016/j.cca.2020.07.025","DOIUrl":"https://doi.org/10.1016/j.cca.2020.07.025","url":null,"abstract":"","PeriodicalId":504940,"journal":{"name":"Clinica chimica acta; international journal of clinical chemistry","volume":" ","pages":"121-122"},"PeriodicalIF":5.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cca.2020.07.025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38162040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV-2 identification and IgA antibodies in saliva: One sample two tests approach for diagnosis.","authors":"Ada Aita,&nbsp;Daniela Basso,&nbsp;Anna Maria Cattelan,&nbsp;Paola Fioretto,&nbsp;Filippo Navaglia,&nbsp;Francesco Barbaro,&nbsp;Alice Stoppa,&nbsp;Enrico Coccorullo,&nbsp;Assunta Farella,&nbsp;Aurora Socal,&nbsp;Roberto Vettor,&nbsp;Mario Plebani","doi":"10.1016/j.cca.2020.09.018","DOIUrl":"https://doi.org/10.1016/j.cca.2020.09.018","url":null,"abstract":"<p><strong>Aim: </strong>This study aims to verify whether standardized saliva collection is suitable for SARS-CoV-2 molecular detection and IgA measurement.</p><p><strong>Methods: </strong>43 COVID-19 inpatients and 326 screening subjects underwent naso-pharyngeal (NP)-swab and saliva collection (Salivette). Inpatients also underwent repeated blood collections to evaluate inflammation and organs involvement. In all patients and subjects, SARS-CoV-2 (gene E) rRT-PCR was undertaken in saliva and NP-swabs. Salivary IgA and serum IgA, IgG, IgM were measured on inpatients' samples.</p><p><strong>Results: </strong>NP-swabs and saliva were both SARS-CoV-2 positive in 7 (16%) or both negative in 35 (82%) out of 43 patients successfully included in the study. NP-swabs and saliva results did not perfectly match in one patient (saliva positive, NP-swab negative). Positive molecular results were significantly associated with disease duration (p = 0.0049). 326/326 screening subjects were SARS-CoV-2 negative on both NP-swabs and saliva. Among the 27 saliva samples tested for IgA, 18 were IgA positive. Salivary IgA positivity was associated with pneumonia (p = 0.002) and CRP values (p = 0.0183), not with other clinical and molecular data, or with serum immunoglubulins.</p><p><strong>Conclusions: </strong>A standardized saliva collection can be adopted to detect SARS-CoV-2 infection in alternative to NP-swabs. Preliminary data on salivary IgA support the use of saliva also for patient monitoring.</p>","PeriodicalId":504940,"journal":{"name":"Clinica chimica acta; international journal of clinical chemistry","volume":" ","pages":"717-722"},"PeriodicalIF":5.0,"publicationDate":"2020-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cca.2020.09.018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38491541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}