两种评估红细胞内镁及其决定因素的方法的比较:来自生命线队列研究的结果。

Joëlle C Schutten, Adrian Post, Margriet van der Meer, Jan IJmker, Frans Goorman, Richard M Danel, Marc G Vervloet, Martin H de Borst, Daan J Touw, Stephan J L Bakker
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引用次数: 7

摘要

背景:评价红细胞内镁(dIEM)的直接方法需要大量的样品制备,使其劳动密集型。另一种劳动强度较小的方法是间接计算红细胞内镁(iIEM)。我们比较了dIEM和iIEM,并在一项基于人群的大型队列研究中研究了dIEM和iIEM的决定因素、血浆镁和24小时尿镁排泄。方法:采用经验证的电感耦合等离子体质谱(ICP-MS)方法对来自生命线队列研究第二次筛选的1669名个体的dIEM和iIEM进行测量。我们使用线性回归分析来研究IEM的决定因素,血浆镁和24小时尿镁排泄。结果:平均dIEM为0.20±0.04 mmol/1012 cells, iem为0.25±0.04 mmol/1012 cells。我们发现dIEM和iIEM之间有很强的相关性(r = 0.75)。Passing-Bablok回归分析显示,截距为0.015 (95% CI: 0.005;0.023),斜率为1.157 (95% CI: 1.109;1.210)。在线性回归分析中,血浆总胆固醇、低密度脂蛋白胆固醇和甘油三酯水平与dIEM、iIEM和血浆镁呈正相关,而葡萄糖和HbA1c与血浆镁呈负相关。结论:我们观察到dIEM和iIEM之间有很强的相关性,表明iIEM是劳动密集型dIEM方法的可靠替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study.

Background: Direct methods for the assessment of intra-erythrocyte magnesium (dIEM) require extensive sample preparation, making them labor intensive. An alternative, less labor intensive method is indirect calculation of intra-erythrocyte magnesium (iIEM). We compared dIEM and iIEM and studied determinants of dIEM and iIEM, plasma magnesium and 24-h urinary magnesium excretion in a large population-based cohort study.

Methods: dIEM and iIEM were measured using a validated inductively coupled plasma mass spectrometry (ICP-MS) method in 1669 individuals from the second screening from the LifeLines Cohort Study. We used linear regression analyses to study the determinants of IEM, plasma magnesium and 24-h urinary magnesium excretion.

Results: Mean dIEM and iIEM were 0.20 ± 0.04 mmol/1012 cells and 0.25 ± 0.04 mmol/1012 cells, respectively. We found a strong correlation between dIEM and iIEM (r = 0.75). Passing-Bablok regression analyses showed an intercept of 0.015 (95% CI: 0.005; 0.023) and a slope of 1.157 (95% CI: 1.109; 1.210). In linear regression analyses, plasma levels of total- and LDL -cholesterol, and triglycerides were positively associated dIEM, iIEM, and plasma magnesium, while glucose and HbA1c were inversely associated with plasma magnesium.

Conclusions: We observed a strong correlation between dIEM and iIEM, suggesting that iIEM is a reliable alternative for the labor intensive dIEM method.

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