European Journal of Histochemistry最新文献_第7页

Pleural mesothelioma from fluoro-edenite exposure: PACAP and PAC1 receptor. A preliminary report. 接触氟烯酸盐导致的胸膜间皮瘤：PACAP 和 PAC1 受体。初步报告。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-05-02 DOI: 10.4081/ejh.2024.3994

Claudia Lombardo, Grazia Maugeri, Agata Grazia D'Amico, Giuseppe Broggi, Rosario Caltabiano, Veronica Filetti, Serena Matera, Velia D'Agata, Carla Loreto

{"title":"Pleural mesothelioma from fluoro-edenite exposure: PACAP and PAC1 receptor. A preliminary report.","authors":"Claudia Lombardo, Grazia Maugeri, Agata Grazia D'Amico, Giuseppe Broggi, Rosario Caltabiano, Veronica Filetti, Serena Matera, Velia D'Agata, Carla Loreto","doi":"10.4081/ejh.2024.3994","DOIUrl":"10.4081/ejh.2024.3994","url":null,"abstract":"Pleural mesothelioma is a devastating malignancy primarily associated with asbestos exposure. However, emerging evidence suggests that exposure to fluoro-edenite fibers, a naturally occurring mineral fiber, can also lead to the development of pleural mesothelioma. In this study, based on the hypothesis that pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP-preferring receptor (PAC1R) expressions could be dysregulated in pleural mesothelioma samples and that they could potentially act as diagnostic or prognostic biomarkers, we aimed to investigate the immunohistochemical expression of PACAP and PAC1R in pleural biopsies from patients with pleural mesothelioma exposed to fluoro-edenite fibers. A total of 12 patients were included in this study, and their biopsies were processed for immunohistochemical analysis to evaluate the expression of PACAP and its receptor. The study revealed a correlation between the overexpression of PACAP and PAC1R and shorter overall survival in patients with malignant mesothelioma. These findings suggest that PACAP and PAC1R expression levels could serve as potential prognostic biomarkers for malignant mesothelioma. Furthermore, the immunohistochemical analysis of PACAP and PAC1R may provide valuable information for clinicians to guide therapeutic decisions and identify patients with poorer prognosis.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11110723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140867138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pretreatment with interleukin-15 attenuates inflammation and apoptosis by inhibiting NF-κB signaling in sepsis-induced myocardial dysfunction. 在脓毒症诱发的心肌功能障碍中，白细胞介素-15预处理可通过抑制NF-κB信号转导减轻炎症和细胞凋亡。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-04-29 DOI: 10.4081/ejh.2024.4019

Chaojie He, Yi Yu, Feifan Wang, Wudi Li, Hui Ni, Meixiang Xiang

{"title":"Pretreatment with interleukin-15 attenuates inflammation and apoptosis by inhibiting NF-κB signaling in sepsis-induced myocardial dysfunction.","authors":"Chaojie He, Yi Yu, Feifan Wang, Wudi Li, Hui Ni, Meixiang Xiang","doi":"10.4081/ejh.2024.4019","DOIUrl":"10.4081/ejh.2024.4019","url":null,"abstract":"Sepsis-induced myocardial dysfunction (SIMD) is associated with poor prognosis and increased mortality in patients with sepsis. Cytokines are important regulators of both the initiation and progression of sepsis. Interleukin-15 (IL-15), a pro-inflammatory cytokine, has been linked to protective effects against myocardial infarction and myocarditis. However, the role of IL-15 in SIMD remains unclear. We established a mouse model of SIMD via cecal ligation puncture (CLP) surgery and a cell model of myocardial injury via lipopolysaccharide (LPS) stimulation. IL-15 expression was prominently upregulated in septic hearts as well as cardiomyocytes challenged with LPS. IL-15 pretreatment attenuated cardiac inflammation and cell apoptosis and improved cardiac function in the CLP model. Similar cardioprotective effects of IL-15 pretreatment were observed in vitro. As expected, IL-15 knockdown had the opposite effect on LPS-stimulated cardiomyocytes. Mechanistically, we found that IL-15 pretreatment reduced the expression of the pro-apoptotic proteins cleaved caspase-3 and Bax and upregulated the anti-apoptotic protein Bcl-2. RNA sequencing and Western blotting further confirmed that IL-15 pretreatment suppressed the activation of nuclear factor kappa B (NF-κB) signaling in mice with sepsis. Besides, the addition of NF-κB inhibitor can significantly attenuate cardiomyocyte apoptosis compared to the control findings. Our results suggest that IL-15 pretreatment attenuated the cardiac inflammatory responses and reduced cardiomyocyte apoptosis by partially inhibiting NF-κB signaling in vivo and in vitro, thereby improving cardiac function in mice with sepsis. These findings highlight a promising therapeutic strategy for SIMD.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11110722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Style head in Apocynaceae: a very complex secretory activity performed by one tissue. 天南星科植物的花柱头：由一个组织进行非常复杂的分泌活动。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-04-02 DOI: 10.4081/ejh.2024.4027

Danielle Maximo, Diego Demarco

{"title":"Style head in Apocynaceae: a very complex secretory activity performed by one tissue.","authors":"Danielle Maximo, Diego Demarco","doi":"10.4081/ejh.2024.4027","DOIUrl":"https://doi.org/10.4081/ejh.2024.4027","url":null,"abstract":"Nuptial glands are very diverse and associated with different pollination mechanisms. The greater the specificity in the pollen transfer mechanism from anther to stigma, the greater the morphological elaboration of flowers and functional complexity of the nuptial glands. In Apocynaceae, pollination mechanisms reached an extreme specificity, a fact that was only possible due to an extreme morphological synorganization and a profusion of floral glands. Although these glands are of different types, the vast majority have secretory cells only in the epidermis. In general, these epidermal cells produce many different compounds at the same time, and previous studies have demonstrated that in the style head, the functional complexity of epidermis has become even greater. Four types of style head are found in the family, which have different degrees of functional complexity in relation to the secretion produced and pollen dispersal mechanism. The secretion is fluid in types I, II and III, and the pollen is dispersed and adhered to the pollinator by the secretion produced by the style head. In type IV, the secretion hardens and acquires a specific shape, moulded by the spatial constraints of the adjacent floral organs. This evolutionary alteration is accompanied by changes in the structure and arrangement of the secretory cells, as well as in pollen aggregation and position of stigma. Histochemical analysis has shown that the secretion is mixed and highly complex, especially in the style head type IV, where the secretion, called translator, is formed by a rigid central portion, which adheres to the pollinator, and two caudicles that attach to two pollinia. The translator has a distinct composition in its different parts. Further studies are needed to answer the new questions that have arisen from the discovery of this highly functional complexity of the secretory tissue.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Feulgen reaction: from pink-magenta to rainbow fluorescent at the Maffo Vialli's School of Histochemistry. 费尔根反应：马福-维亚利组织化学学院从粉红-洋红到彩虹荧光。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-02-22 DOI: 10.4081/ejh.2024.3971

Giuliano Mazzini

{"title":"The Feulgen reaction: from pink-magenta to rainbow fluorescent at the Maffo Vialli's School of Histochemistry.","authors":"Giuliano Mazzini","doi":"10.4081/ejh.2024.3971","DOIUrl":"10.4081/ejh.2024.3971","url":null,"abstract":"For over a century, Palazzo Botta (Palace Botta) has housed the University of Pavia's Biomedical Institutes. Illustrious scientists have conducted research and taught at this Palace, making significant contributions to the advancement of natural, biological, and medical science. Among them, Camillo Golgi received the Nobel Prize for discovering the so-called \"black reaction.\" Following Golgi, the Palace continued to be a hub for the development of methodologies and reactions aimed at detecting and quantifying biological components. Maffo Vialli (in the Golgi stream) was the first to establish a Histochemistry Research Group, which began in the naturalistic field and later expanded to the biomedical area. Among the many histochemical studies initiated in the Palace, the Feulgen reaction undoubtedly played a significant role. This reaction, developed R. Feulgen and H. Rossenbeck in 1924, had significant international implications: numerous researchers then contributed to define its fine chemical details, which remained the subject of study for years, resulting in a massive international scientific literature. The Pavia School of Histochemistry also contributed to the evolution and application of this method, which has become a true benchmark in quantitative histochemistry. Giovanni Prenna and the CNR Centre for Histochemistry made significant contributions, as they were already focused on fluorescence cytochemistry. The Pavia researchers made significant contributions to the development of methodology and, in particular, instrumentation; the evolution of the latter resulted in the emergence of flow cytometry and an ever-increasing family of fluorescent probes, which somewhat overshadowed the Feulgen reaction for DNA quantification. The advent of monoclonal antibodies then contributed to the final explosion of flow cytometry in clinical application, almost making young neophytes forget that its roots date back to Feulgen.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11059467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139933979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lectins as versatile tools to explore cellular glycosylation. 连接蛋白是探索细胞糖基化的多功能工具。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-01-29 DOI: 10.4081/ejh.2024.3959

Susan Brooks

{"title":"Lectins as versatile tools to explore cellular glycosylation.","authors":"Susan Brooks","doi":"10.4081/ejh.2024.3959","DOIUrl":"10.4081/ejh.2024.3959","url":null,"abstract":"Lectins are naturally occurring carbohydrate-binding proteins that are ubiquitous in nature and highly selective for their, often incompletely characterised, binding partners. From their discovery in the late 1880s to the present day, they have provided a broad palette of versatile tools for exploring the glycosylation of cells and tissues and for uncovering the myriad functions of glycosylation in biological systems. The technique of lectin histochemistry, used to map the glycosylation of tissues, has been instrumental in revealing the changing profile of cellular glycosylation in development, health and disease. It has been especially enlightening in revealing fundamental alterations in cellular glycosylation that accompany cancer development and metastasis, and has facilitated the identification of glycosylated biomarkers that can predict prognosis and may have utility in development of early detection and screening, Moreover, it has led to insights into the functional role of glycosylation in healthy tissues and in the processes underlying disease. Recent advances in biotechnology mean that our understanding of the precise binding partners of lectins is improving and an ever-wider range of lectins are available, including recombinant human lectins and lectins with enhanced, engineered properties. Moreover, use of traditional histochemistry to support a broad range of cutting-edge technologies and the development of high throughout microarray platforms opens the way for ever more sophisticated mapping - and understanding - of the glycome.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11059468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139571940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

1954-2024: 70 years of histochemical research with the European Journal of Histochemistry. 1954-2024：《欧洲组织化学杂志》组织化学研究 70 年。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-01-29 DOI: 10.4081/ejh.2024.3970

Carlo Pellicciari, Marco Biggiogera, Manuela Malatesta

引用次数: 0

Giving bodies to ghosts: locating molecules in the very place where they exert their biological roles. 为幽灵赋予躯体：将分子定位在其发挥生物作用的地方。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-01-29 DOI: 10.4081/ejh.2024.3950

Ernesto Capanna, CarloAlberto Redi

{"title":"Giving bodies to ghosts: locating molecules in the very place where they exert their biological roles.","authors":"Ernesto Capanna, CarloAlberto Redi","doi":"10.4081/ejh.2024.3950","DOIUrl":"10.4081/ejh.2024.3950","url":null,"abstract":"This paper reviews some of the goals of our investigations published over the years on Rivista di Istochimica Normale e Patologica, Basic and Applied Histochemistry, and the European Journal of Histochemistry - EJH. In a series of papers, we published some of the basic cytochemical features of the sperm cytodifferentiation process for the first time. This was a conceptual and practical prerequisite to the in situ quantitative evaluation of sperm DNA content. We showed that the discrepancy between the expected 1:2 ratio when comparing sperm versus somatic cell DNA content (sperm DNA content is always far low from the theoretical value) is due to DNA losses caused by the hydrochloric treatment entailed by the Feulgen reaction. The knowledge of the specific losses that occur during the various steps of the Feulgen reaction has allowed us to use it critically in Genome Size studies to highlight: - sperm aneuploidy in chromosomally derived subfertility; - the broad variability range of Mammalian genome sizes; - that termites are roaches (after decades of discussion on this topic). In addition, in a seminal paper on human oocytes, we showed (by transmission electron microscopy) a specific chromatin and cytoplasmic organization (both essential for further embryo development) linked to oocyte maturation arrest, a datum quite relevant to treating unmet therapeutic needs in human and veterinary reproduction.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11059455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139571937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SNORA38B promotes proliferation, migration, invasion and epithelial-mesenchymal transition of gallbladder cancer cells via activating TGF-β/Smad2/3 signaling SNORA38B 通过激活 TGF-β/Smad2/3 信号促进胆囊癌细胞的增殖、迁移、侵袭和上皮-间质转化

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2023-12-28 DOI: 10.4081/ejh.2023.3899

Yiyu Qin, Jian Li, Hongchao Han, Yongliang Zheng, Haiming Lei, Yang Zhou, Hongyan Wu, Guozhe Zhang, Xiang Chen, Zhengping Chen

{"title":"SNORA38B promotes proliferation, migration, invasion and epithelial-mesenchymal transition of gallbladder cancer cells via activating TGF-β/Smad2/3 signaling","authors":"Yiyu Qin, Jian Li, Hongchao Han, Yongliang Zheng, Haiming Lei, Yang Zhou, Hongyan Wu, Guozhe Zhang, Xiang Chen, Zhengping Chen","doi":"10.4081/ejh.2023.3899","DOIUrl":"https://doi.org/10.4081/ejh.2023.3899","url":null,"abstract":"Evidence has shown that small nucleolar RNAs (snoRNAs) participate in the tumorigenesis in multiple cancers, including gallbladder cancer (GBC). Our results showed that SNORA38B level was increased in GBC tissues compared to adjacent normal tissues. Thus, this research aimed to explore the role and molecular mechanisms of SNORA38B in GBC. SNORA38B level between normal and GBC tissues was evaluated by RT-qPCR. Cell proliferation, apoptosis, migration, and invasion were tested by EdU assay, TUNEL staining and transwell assay, respectively on human intrahepatic biliary epithelial cells (HIBEpiCs) and the GBC cell lines, NOZ and GBC-SD. Expression of proteins in GBC cells was evaluated by immunofluorescence and Western blot assays. We found that, relative to normal tissues, SNORA38B level was notably elevated in GBC tissues. SNORA38B overexpression obviously enhanced GBC cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT), but weakened cell apoptosis. Conversely, SNORA38B downregulation strongly suppressed the proliferation and EMT of GBC cells and induced cell apoptosis and ferroptosis, whereas these phenomena were obviously reversed by TGF-β. Meanwhile, SNORA38B downregulation notably reduced the levels of phosphorylated-Smad2 and phosphorylated-Smad3 in GBC cells, whereas these levels were elevated by TGF-β. Collectively, downregulation of SNORA38B could inhibit GBC cell proliferation and EMT and induce ferroptosis via inactivating TGF-β1/Smad2/3 signaling. These findings showed that SNORA38B may be potential target for GBC treatment.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"1 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139150738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of seasonal changes in lipid synthesis and metabolism-related genes in the oviduct of Chinese brown frog (Rana dybowskii). 中国褐蛙输卵管脂质合成及代谢相关基因的季节性变化研究

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2023-12-20 DOI: 10.4081/ejh.2023.3890

Yankun Wang, Yuning Liu, Yawei Wang, Ao Zhang, Wenqian Xie, Haolin Zhang, Qiang Weng, Meiyu Xu

{"title":"Investigation of seasonal changes in lipid synthesis and metabolism-related genes in the oviduct of Chinese brown frog (Rana dybowskii).","authors":"Yankun Wang, Yuning Liu, Yawei Wang, Ao Zhang, Wenqian Xie, Haolin Zhang, Qiang Weng, Meiyu Xu","doi":"10.4081/ejh.2023.3890","DOIUrl":"10.4081/ejh.2023.3890","url":null,"abstract":"A peculiar physiological characteristic of the Chinese brown frog (Rana dybowskii) is that its oviduct dilates during pre-brumation rather than during the breeding season. This research aimed to examine the expression of genes connected with lipid synthesis and metabolism in the oviduct of R. dybowskii during both the breeding season and pre-brumation. We observed significant changes in the weight and size of the oviduct between the breeding season and pre-brumation. Furthermore, compared to the breeding season, pre-brumation exhibited significantly lower triglyceride content and a marked increase in free fatty acid content. Immunohistochemical results revealed the spatial distribution of triglyceride synthase (Dgat1), triglyceride hydrolase (Lpl and Hsl), fatty acid synthase (Fasn), and fatty acid oxidases (Cpt1a, Acadl, and Hadh) in oviductal glandular cells and epithelial cells during both the breeding season and pre-brumation. While the mRNA levels of triglycerides and free fatty acid synthesis genes (dgat1 and fasn) did not show a significant difference between the breeding season and pre-brumation, the mRNA levels of genes involved in triglycerides and free fatty acid metabolism (lpl, cpt1a, acadl, acox and hadh) were considerably higher during pre-brumation. Furthermore, the R. dybowskii oviduct's transcriptomic and metabolomic data confirmed differential expression of genes and metabolites enriched in lipid metabolism signaling pathways during both the breeding season and pre-brumation. Overall, these results suggest that alterations in lipid synthesis and metabolism during pre-brumation may potentially influence the expanding size of the oviduct, contributing to the successful overwintering of R. dybowskii.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"67 4","pages":""},"PeriodicalIF":2.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10773197/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138812754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Curculigoside inhibits osteoarthritis via the regulation of NLRP3 pathway. 莪术苷通过调节 NLRP3 通路抑制骨关节炎

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2023-12-13 DOI: 10.4081/ejh.2023.3896

Xufei Wang, Yinlian Liu, Yongnian Zhou, Yang Zhou, Yueping Li

{"title":"Curculigoside inhibits osteoarthritis via the regulation of NLRP3 pathway.","authors":"Xufei Wang, Yinlian Liu, Yongnian Zhou, Yang Zhou, Yueping Li","doi":"10.4081/ejh.2023.3896","DOIUrl":"10.4081/ejh.2023.3896","url":null,"abstract":"Osteoarthritis (OA) is characterized by degenerative articular cartilage. Nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 (NLRP3) plays an important role in inflammation. This study aims to investigate whether protective effects of curculigoside on OA are medicated by the regulation of NLRP3 pathway. Destabilization of the medial meniscus (DMM) was performed to build an OA mouse model. After surgery, OA mice were treated with curculigoside. Immunohistochemistry was conducted to evaluate OA cartilage. In addition, human chondrocytes were isolated and treated with curculigoside. The mRNA and protein expression of iNOS, MMP-9, NLRP3 was detected by PCR and Western blot analysis. Curculigoside inhibited mRNA and protein levels of iNOS and MMP-9 induced by DMM surgery in a dose-dependent manner. Furthermore, the expression of NLRP3, NF-κB and PKR was downregulated after curculigoside administration. Moreover, curculigoside reversed the effects of IL-1β on MMP-9, iNOS and type II collagen expression at mRNA and protein levels in human chondrocytes in a dose-dependent manner. In conclusion, curculigoside exhibits beneficial effect on cartilage via the inhibition of NLRP3 pathway.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"67 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10773194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138812751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0