Indian Journal of Virology最新文献_第5页

Molecular Evidence for Occurrence of Tomato leaf curl New Delhi virus in Ash Gourd (Benincasa hispida) Germplasm Showing a Severe Yellow Stunt Disease in India. 在印度出现严重黄萎病的灰葫芦（Benincasa hispida）种质中发现番茄卷叶新德里病毒的分子证据。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2012-11-03 DOI: 10.1007/s13337-012-0115-y

Anirban Roy, P Spoorthi, G Panwar, Manas Kumar Bag, T V Prasad, Gunjeet Kumar, K K Gangopadhyay, M Dutta

{"title":"Molecular Evidence for Occurrence of Tomato leaf curl New Delhi virus in Ash Gourd (Benincasa hispida) Germplasm Showing a Severe Yellow Stunt Disease in India.","authors":"Anirban Roy, P Spoorthi, G Panwar, Manas Kumar Bag, T V Prasad, Gunjeet Kumar, K K Gangopadhyay, M Dutta","doi":"10.1007/s13337-012-0115-y","DOIUrl":"10.1007/s13337-012-0115-y","url":null,"abstract":"An evaluation of 70 accessions of ash gourd germplasm grown at National Bureau of Plant Genetic Resources, New Delhi, India during Kharif season (2010) showed natural occurrence of a yellow stunt disease in three accessions (IC554690, IC036330 and Pusa Ujjwal). A set of begomovirus specific primers used in PCR gave expected amplicon from all the symptomatic plants; however no betasatellite was detected. Complete genome of the begomovirus (DNA-A and DNA-B), amplified through rolling circle amplification, was cloned and sequenced. The begomovirus under study shared high sequence identities to different isolates of Tomato leaf curl New Delhi virus (ToLCNDV) and clustered with them. Among those isolates, the DNA-A and DNA-B of the present begomovirus isolate showed highest 99.6 and 96.8 % sequence identities, respectively with an isolate reported on pumpkin from India (DNA-A: AM286433, DNA-B: AM286435). Based on the sequence analysis, the begomovirus obtained from ash gourd was considered as an isolate of ToLCNDV. Thus, the present findings constitute the first report of occurrence of a new yellow stunt disease in ash gourd from India and demonstrated the association of ToLCNDV with the symptomatic samples. Occurrence of ToLCNDV in ash gourd germplasm not only adds up a new cucurbitaceous host of this virus but also raises the concern about the perpetuation of this virus in absence of its main host tomato and thus has an epidemiological relevance for understanding the rapid spread of this virus in tomato and other hosts in Indian sub-continent. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"24 1","pages":"74-7"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3650187/pdf/13337_2012_Article_115.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32032445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

VP292 of White spot syndrome virus Interacts with VP26. 白斑综合征病毒VP292与VP26的相互作用

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2012-12-16 DOI: 10.1007/s13337-012-0111-2

Qian Li, Qing-Hui Liu, Jie Huang

引用次数: 5

Isolation and Sequence Analysis of Highly Pathogenic Porcine reproductive and respiratory syndrome virus from Swine Herds in the Jilin Province of China. 吉林省猪群高致病性猪繁殖与呼吸综合征病毒的分离与序列分析

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2013-01-12 DOI: 10.1007/s13337-012-0124-x

Feng-Xue Wang, Li Guo, Yan-Ling Yang, Ni Song, Li-Zhi Chen, Shi-Peng Cheng, Yong-Jun Wen

{"title":"Isolation and Sequence Analysis of Highly Pathogenic Porcine reproductive and respiratory syndrome virus from Swine Herds in the Jilin Province of China.","authors":"Feng-Xue Wang, Li Guo, Yan-Ling Yang, Ni Song, Li-Zhi Chen, Shi-Peng Cheng, Yong-Jun Wen","doi":"10.1007/s13337-012-0124-x","DOIUrl":"https://doi.org/10.1007/s13337-012-0124-x","url":null,"abstract":"The aim of the present study was to determine the causative agent of infected swines in the Jilin province of China and assess its genetic characteristics. Virus was isolated from tissues suspected of being infected by porcine reproductive and respiratory syndrome virus (PRRSV) and inoculated onto MARC-145 cells. Virus detection was carried out by RT-PCR, immunofluorescence, electron microscopy and sequencing. The results showed that the isolate was the North American genotype PRRSV, termed the JL-04/12 strain, with a 15,320 bp genome. The homology of the amino acid sequences in two nonstructural proteins and GP2 to GP5, between strains JL-04/12 and HUN4, ranged from 97.2 to 99.3 %. However, JL-04/12 GP6 and N protein were identical in HP-PRRSV JXA1 and HUN4. JL-04/12 was characterized by two discontinuous deletions in Nsp2. We speculate that the isolate is a variant of highly pathogenic porcine reproductive and respiratory syndrome derived from strains in 2006-2008. Altogether, these results indicate that highly pathogenic porcine reproductive and respiratory syndrome virus still exists in the Jilin province of China. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"24 1","pages":"90-2"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-012-0124-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32029591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evidence of Grapevine leafroll associated virus-1-3, Grapevine fleck virus and Grapevine virus B Occurring in Himachal Pradesh, India. 印度喜马偕尔邦出现葡萄花叶病相关病毒-1-3、葡萄斑点病毒和葡萄病毒 B 的证据。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2013-02-15 DOI: 10.1007/s13337-013-0129-0

Surender Kumar, Lakhmir Singh, Luca Ferretti, Marina Barba, Aijaz A Zaidi, Vipin Hallan

{"title":"Evidence of Grapevine leafroll associated virus-1-3, Grapevine fleck virus and Grapevine virus B Occurring in Himachal Pradesh, India.","authors":"Surender Kumar, Lakhmir Singh, Luca Ferretti, Marina Barba, Aijaz A Zaidi, Vipin Hallan","doi":"10.1007/s13337-013-0129-0","DOIUrl":"10.1007/s13337-013-0129-0","url":null,"abstract":"During a survey conducted in the grapevine orchards of Himachal Pradesh, variety of symptoms ranging from leaf yellowing, vein greening, reduced leaf size, downward rolling/cup shaped leaves to reduced fruit bearing were observed. Symptomatic leaf samples were collected and analyzed by serological (DAS-ELISA) and molecular methods (RT-PCR, PCR) for viruses and phytoplasma known worldwide on grapevine. DAS-ELISA was used for detection of Grapevine leafroll associated virus 1, 2 and 3 (GLRaV-1, 2 & 3), Grapevine virus A (GVA), Grapevine fan leaf virus (GFLV), Grapevine fleck virus (GFkV) and successfully detected GLRaV-1 & 3 and GFkV. All these samples were complemented with RT- PCR along with GVb and phytoplasma (additional to ELISA) using specific primers. Specific amplification in RT-PCR for GLRaV-1 (~232 bp), GLRaV-3 (~300 bp), GFkV (~179 bp) and GVB (~440 bp) confirmed the presence of these pathogens. Overall, ELISA and RT-PCR results confirmed the presence GLRaV-3 (66.7 %), GLRaV-1& GFkV (50 %), and Grapevine virus B (GVB) (12.5 %) in symptomatic plants. None of the samples were found positive for GFLV, GLRaV-2 and phytoplasma. Mixed infection was common and none of the plants were found virus free. To the best of our knowledge this is the first report of detection of GFkV and GVB in India. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"24 1","pages":"66-9"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3650186/pdf/13337_2013_Article_129.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32032443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural Occurrence of Banana bunchy top virus in Ensete superbum in India. 香蕉束顶病毒在印度的自然发生。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2013-01-10 DOI: 10.1007/s13337-012-0123-y

R Selvarajan, V Balasubramanian

引用次数: 14

First Report on Occurrence of Pelargonium leaf curl virus (PLCV) in Shimla Hills, India. 印度西姆拉山发生天竺葵卷叶病毒(PLCV)首次报道。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2013-01-10 DOI: 10.1007/s13337-012-0121-0

Deep Kumar, Priyanka Kaundal, I D Garg

引用次数: 3

Viruses occurring in onion crop in amasya province, the major onion producing region in Turkey. 土耳其洋葱主产区阿马西亚省洋葱作物中出现的病毒。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2012-12-16 DOI: 10.1007/s13337-012-0114-z

Mehmet Ali Sevik, Cemile Akcura

引用次数: 0

Immune regulation and evasion of Mammalian host cell immunity during viral infection. 病毒感染过程中哺乳动物宿主细胞免疫的免疫调节和逃避。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2013-03-15 DOI: 10.1007/s13337-013-0130-7

B M Pratheek, Soham Saha, Prasanta K Maiti, Soma Chattopadhyay, Subhasis Chattopadhyay

{"title":"Immune regulation and evasion of Mammalian host cell immunity during viral infection.","authors":"B M Pratheek, Soham Saha, Prasanta K Maiti, Soma Chattopadhyay, Subhasis Chattopadhyay","doi":"10.1007/s13337-013-0130-7","DOIUrl":"https://doi.org/10.1007/s13337-013-0130-7","url":null,"abstract":"The mammalian host immune system has wide array of defence mechanisms against viral infections. Depending on host immunity and the extent of viral persistence, either the host immune cells might clear/restrict the viral load and disease progression or the virus might evade host immunity by down regulating host immune effector response(s). Viral antigen processing and presentation in the host cells through major histocompatibility complex (MHC) elicit subsequent anti-viral effector T cell response(s). However, modulation of such response(s) might generate one of the important viral immune evasion strategies. Viral peptides are mostly generated by proteolytic cleavage in the cytosol of the infected host cells. CD8(+) T lymphocytes play critical role in the detection of viral infection by recognizing these peptides displayed at the plasma membrane by MHC-I molecules. The present review summarises the current knowledge on the regulation of mammalian host innate and adaptive immune components, which are operative in defence mechanisms against viral infections and the variety of strategies that viruses have evolved to escape host cell immunity. The understanding of viral immune evasion strategies is important for designing anti-viral immunotherapies. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"24 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-013-0130-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32031369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Evidence of Recombinant Citrus tristeza virus Isolate Occurring in Acid Lime cv. Pant Lemon Orchard in Uttarakhand Terai Region of Northern Himalaya in India. 重组柑橘tristeza病毒分离株在酸性石灰cv中发生的证据。印度北喜马拉雅的北阿坎德邦Terai地区的潘特柠檬果园。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2012-12-18 DOI: 10.1007/s13337-012-0118-8

Jaywant Kumar Singh, Avijit Tarafdar, Susheel Kumar Sharma, Kajal Kumar Biswas

{"title":"Evidence of Recombinant Citrus tristeza virus Isolate Occurring in Acid Lime cv. Pant Lemon Orchard in Uttarakhand Terai Region of Northern Himalaya in India.","authors":"Jaywant Kumar Singh, Avijit Tarafdar, Susheel Kumar Sharma, Kajal Kumar Biswas","doi":"10.1007/s13337-012-0118-8","DOIUrl":"https://doi.org/10.1007/s13337-012-0118-8","url":null,"abstract":"The present study for the first time describes biological and molecular characterization of Citrus tristeza virus (CTV) occurring in the Terai area of Uttarakhand State in Northern Himalaya region of India. Direct antigen coated-ELISA and reverse transcriptase-polymerase chain reaction (RT-PCR) detected the CTV infection in Acid lime cv. Pant lemon (Citrus aurantifolia) orchards of Pantnagar with an estimated disease incidence of 16.6-20.5 %. To know the biological and genetic properties, an isolate, CTV Pant 4 was characterized. Isolate Pant 4 could be graft transmitted to Kinnow, Nagpur and Darjeeling mandarins, Mosambi sweet orange, Kagzi lime, Sweet lime, Sour orange but not to Rough lemon. The sequence analyses of the 5'ORF1a (3038 nucleotides) of LPro domain and 3'end (2058 nt) covering ORF7-ORF10 regions of the CTV genome revealed that Pant 4 was closely related to the previously reported Indian CTV isolate, Kpg3 from Northeastern Himalaya region with 97 and 98 % sequence identity, respectively. Whereas, it differed from the previously reported CTV isolate B165 from Southern India with 79 and 92 % identity, respectively for 5'ORF1a and 3' end regions. Recombination and SplitsTree decomposition analyses indicated that CTV isolate Pant 4 was a recombinant isolate originating from Kpg3 as a major and B165 as a minor donor. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"24 1","pages":"35-41"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-012-0118-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32031370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Molecular Characterization of Thermostable Newcastle disease virus Isolated from Pigeon. 鸽源耐热性新城疫病毒的分子特性研究。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2013-01-29 DOI: 10.1007/s13337-012-0120-1

A Uthrakumar, K Vijayarani, K Kumanan, V Thiagarajan

引用次数: 7