白斑综合征病毒VP292与VP26的相互作用

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2012-12-16 DOI:10.1007/s13337-012-0111-2
Qian Li, Qing-Hui Liu, Jie Huang
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引用次数: 5

摘要

病毒结构蛋白之间的相互作用被认为是病毒组装的关键。白斑综合征病毒(WSSV)组装和成熟过程的许多步骤尚不清楚。在本文中,我们发现了WSSV VP292的一个新的相互作用。时间转录分析表明,VP292在WSSV感染后期表达。Western blot和基质辅助激光解吸电离质谱分析表明,VP292与主要包膜蛋白VP26相互作用。Far-western blot进一步证明了VP292和VP26之间的相互作用。这些结果共同表明VP292通过与VP26的相互作用锚定在包膜上。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
VP292 of White spot syndrome virus Interacts with VP26.

Interactions between virus structural proteins are suggested to be crucial for virus assembly. Many steps in the process of white spot syndrome virus (WSSV) assembly and maturation remain unclear. In this paper, we discovered a new interaction of WSSV VP292. Temporal-transcription analysis showed that VP292 is expressed in the late stage of WSSV infection. Western blot and matrix-assisted laser desorption ionization MS assays showed that VP292 interacts with VP26, a major envelope protein. Far-western blot provided further evidence for interaction between VP292 and VP26. These results collectively demonstrated that VP292 anchors to the envelope through interaction with VP26.

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来源期刊
Indian Journal of Virology
Indian Journal of Virology 医学-病毒学
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6-12 weeks
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