Indian Journal of Virology最新文献_第6页

Genetic Diversity Based on Coat Protein of Papaya ringspot virus (Pathotype P) Isolates from Bangladesh. 基于孟加拉国木瓜环斑病毒（病原型 P）分离株外壳蛋白的遗传多样性。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2013-02-12 DOI: 10.1007/s13337-013-0127-2

M S Akhter, Y B Basavaraj, A M Akanda, B Mandal, R K Jain

引用次数: 0

Development of a probe based real time PCR assay for detection of bovine herpes virus-1 in semen and other clinical samples. 开发基于探针的实时 PCR 检测法，用于检测精液和其他临床样本中的牛疱疹病毒-1。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2012-12-16 DOI: 10.1007/s13337-012-0112-1

Basavegowdanadoddi Marinaik Chandranaik, Doddamane Rathnamma, S S Patil, Ramesh C Kovi, Jyotsana Dhawan, Shakunigowda Ranganatha, Shrikrishna Isloor, C Renukaprasad, K Prabhudas

{"title":"Development of a probe based real time PCR assay for detection of bovine herpes virus-1 in semen and other clinical samples.","authors":"Basavegowdanadoddi Marinaik Chandranaik, Doddamane Rathnamma, S S Patil, Ramesh C Kovi, Jyotsana Dhawan, Shakunigowda Ranganatha, Shrikrishna Isloor, C Renukaprasad, K Prabhudas","doi":"10.1007/s13337-012-0112-1","DOIUrl":"10.1007/s13337-012-0112-1","url":null,"abstract":"This study describes development of a TaqMan probe based real time PCR assay that can detect BoHV-1 of as low as 0.001 TCID50/0.1 ml in clinical samples, its comparative evaluation with indirect ELISA and virus isolation for detection of Bovine herpes virus-1 (BoHV-1) in semen and swab clinical samples. For this study, we collected samples from 212 animals (cattle and buffaloes) comprising 91 bulls and 121 females. Avidin-biotin ELISA employed on serum samples from 212 animals revealed 74 as seropositive for BoHV-1. On inoculation of semen/swabs on MDBK cell line, nine samples yielded cytopathic changes characteristic of herpes viruses. The isolates were confirmed by VNT and a conventional PCR. A real time PCR assay was standardised by designing a new set of TaqMan probe and primers targeting a 71 bp region on gB gene of the virus. The assay detected viral antigen in 21 seropositive and 14 seronegative animals, emphasizing the relevance of serology in BoHV-1 diagnosis, particularly in breeding stations. Further, real time PCR assay was 100 % sensitive and 87.19 % specific compared to virus isolation in detection of the BoHV-1 in clinical samples. The assay was validated at reputed national laboratories, with a sensitivity of ≥99 %. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"24 1","pages":"16-26"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3650191/pdf/13337_2012_Article_112.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32031367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber. 引起黄瓜严重花叶病的黄瓜花叶病毒 II 亚群分离株的生物学和分子特征。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2013-01-17 DOI: 10.1007/s13337-012-0125-9

Reenu Kumari, Pooja Bhardwaj, Lakhmir Singh, Aijaz A Zaidi, Vipin Hallan

{"title":"Biological and Molecular Characterization of Cucumber mosaic virus Subgroup II Isolate Causing Severe Mosaic in Cucumber.","authors":"Reenu Kumari, Pooja Bhardwaj, Lakhmir Singh, Aijaz A Zaidi, Vipin Hallan","doi":"10.1007/s13337-012-0125-9","DOIUrl":"10.1007/s13337-012-0125-9","url":null,"abstract":"Cucumber mosaic virus (CMV) has a wide host range causing severe damage in many important agricultural and ornamental crops. Earlier reports showed the prevalence of CMV subgroup I isolates in India. However, some recent reports point towards increasing incidence of subgroup II isolates in the country. The complete genome of a CMV isolate causing severe mosaic in cucumber was characterized and its phylogenetic analysis with other 21 CMV isolates reported worldwide clustered it with subgroup II strains. The genome comprised of RNA 1 (3,379 nucleotides), RNA 2 (3,038 nucleotides) and RNA 3 (2,206 nucleotides). The isolate showed highest homology with subgroup II isolates: 95.1-98.7, 87.7-98.0, and 85.4-97.1 % within RNA1, RNA2, and RNA3, respectively. RNA1 and RNA2 were closely related to the Japanese isolate while RNA3 clustered with an American isolate. Host range studies revealed that isolate showed severe mosaic symptoms on Nicotiana spp. and Cucumis spp. The isolate induced leaf deformation and mild filiform type symptoms in tomato. To best of our knowledge this is the first report of complete genome of CMV subgroup II isolate from India. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"24 1","pages":"27-34"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3650197/pdf/13337_2012_Article_125.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32031368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of nucleopolyhedrovirus of three lepidopteran pests using late expression factor-8 gene. 用晚表达因子-8基因对三种鳞翅目害虫核多角体病毒的分子特征分析。

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2013-01-29 DOI: 10.1007/s13337-013-0126-3

Jency Jose, S K Jalali, T M Shivalingaswamy, N K Krishna Kumar, R Bhatnagar, A Bandyopadhyay

{"title":"Molecular characterization of nucleopolyhedrovirus of three lepidopteran pests using late expression factor-8 gene.","authors":"Jency Jose, S K Jalali, T M Shivalingaswamy, N K Krishna Kumar, R Bhatnagar, A Bandyopadhyay","doi":"10.1007/s13337-013-0126-3","DOIUrl":"https://doi.org/10.1007/s13337-013-0126-3","url":null,"abstract":"A PCR based method for detection of viral DNA in nucleopolyhedrovirus of three lepidopterans, Spodoptera litura, Amsacta albistriga and Helicoverpa armigera, was developed by employing the late expression factor-8 (lef-8) gene of three NPV using specific primers. The amplicons of 689, 699 and 665 bp were amplified, respectively, and the nucleotide sequences were submitted to GenBank and the accession numbers were obtained. The sequences of lef-8 gene of S. litura NPV and H. armigera NPV matched with those of their respective references in the GenBank database, thereby confirming their identity, however, the sequence of A. albistriga NPV was the first sequence submitted to the GenBank database. The sequence similarity analysis between the three lef-8 gene of NPV sequenced in the present study revealed that there was no significant similarity between them, however A. albistriga NPV and S. litura NPV were found to be closely related. CLUSTAL alignment of the sequences generated revealed general relatedness among NPVs lef-8 gene. The study confirmed that lef-8 gene can be used for quick and correct discriminatory identification of insect viruses. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"24 1","pages":"59-65"},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-013-0126-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32032442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Main Factors Influencing the Efficient Propagation of Virulent or Attenuated Strains of Japanese encephalitis virus in BHK-21 Cells. 影响乙型脑炎病毒强毒株和弱毒株在BHK-21细胞内高效繁殖的主要因素

Indian Journal of Virology Pub Date : 2012-12-01 Epub Date: 2012-09-12 DOI: 10.1007/s13337-012-0094-z

Man Teng, Jun Luo, Guang-Xu Xing, Na Cheng, Yan-Yan Yang, Rui-Guang Deng, Gai-Ping Zhang

引用次数: 3

Newcastle disease virus in pakistan: genetic characterization and implication in molecular diagnosis. 巴基斯坦新城疫病毒:遗传特征及其在分子诊断中的意义。

Indian Journal of Virology Pub Date : 2012-12-01 Epub Date: 2012-07-04 DOI: 10.1007/s13337-012-0073-4

M Munir, S Zohari, M Berg

{"title":"Newcastle disease virus in pakistan: genetic characterization and implication in molecular diagnosis.","authors":"M Munir, S Zohari, M Berg","doi":"10.1007/s13337-012-0073-4","DOIUrl":"https://doi.org/10.1007/s13337-012-0073-4","url":null,"abstract":"Newcastle disease (ND) is a fatal and contagious disease that poses a constant threat to the poultry industry around the globe. Due to the complex clinico-pathological picture and high genetic variability, the efficient diagnosis of NDV strains is a challenge. In an emerging wave of ND in the north of Pakistan, samples from six outbreaks in commercial poultry and two from healthy backyard poultry flocks were screened for NDV. A real-time PCR based on the fusion and polymerase genes of NDV detected all six isolates whereas a validated real-time PCR based on the matrix gene failed to detect any of these isolates, most likely due to substantial mismatches in the probe-binding site. All isolates have shown ICPI and MDT values similar to the velogenic form of NDV strains. The cleavage site in the F protein was found to be (112)RRQKR↓F(117), typical of virulent NDV. Phylogenetic reconstruction, based on fusion and matrix genes, provided enough evidences to consider these isolates as a new subgenotype within genotype VII. This study raised concerns about the genetic variability of NDV circulating in Pakistan, and sensitivity of the assays for the detection of the NDV isolates in clinical samples. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"23 3","pages":"368-73"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-012-0073-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31916994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Enteroviral central nervous system infections in children of the region of monastir, Tunisia: diagnosis, laboratory findings of cerebrospinal fluid and clinical manifestations. 突尼斯monastir地区儿童肠病毒中枢神经系统感染:诊断、脑脊液实验室检查结果和临床表现

Indian Journal of Virology Pub Date : 2012-12-01 Epub Date: 2012-09-04 DOI: 10.1007/s13337-012-0104-1

Raida El Hiar, Samir Haddad, Hela Jaïdane, Didier Hober, Manel Ben M'hadheb-Gharbi, Maria Gullberg, Mohamed Neji-Guediche, A Michael Lindberg, Jawhar Gharbi, Mahjoub Aouni

{"title":"Enteroviral central nervous system infections in children of the region of monastir, Tunisia: diagnosis, laboratory findings of cerebrospinal fluid and clinical manifestations.","authors":"Raida El Hiar, Samir Haddad, Hela Jaïdane, Didier Hober, Manel Ben M'hadheb-Gharbi, Maria Gullberg, Mohamed Neji-Guediche, A Michael Lindberg, Jawhar Gharbi, Mahjoub Aouni","doi":"10.1007/s13337-012-0104-1","DOIUrl":"https://doi.org/10.1007/s13337-012-0104-1","url":null,"abstract":"Human enteroviruses (HEV) are one of the major causes of central nervous system (CNS) infections in pediatrics. A prospective study was conducted to assess the epidemiological, clinical, and laboratory characteristics of enterovirus (EV) infections of the CNS in children under 15-years-old, suspected of having viral CNS infections and admitted to the Pediatric Department of Monastir University Hospital, Tunisia. Enteroviral RNA was detected by 5' NCR nested RT-PCR assay in 33 % (20 out of 60) of cerebrospinal fluid specimens, whereas only six samples (10 %) were EV positive in cell culture. EV-positive patients were clustered according to their clinical manifestations, predominantly diagnosed as aseptic meningitis (65 %) and meningoencephalitis (20 %). Fever, headache, vomiting, and neck stiffness were the most pronounced symptoms. Pleocytosis with the predominance of lymphocytes was observed in 60 % of EV positive specimens. Although patients suffering from EV infections were encountered throughout the year, most occurred during spring and summer months. Using VP1-2A nested RT-PCR and sequence analysis, three of the 20 positive HEV were identified as Echovirus (E)-9. This is the first report of a cluster of aseptic meningitis cases caused by E-9 in Monastir. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"23 3","pages":"294-302"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-012-0104-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31918079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Recent Advances in Development of DNA Vaccines Against Hepatitis C virus. 丙型肝炎病毒DNA疫苗的研究进展

Indian Journal of Virology Pub Date : 2012-12-01 Epub Date: 2012-03-20 DOI: 10.1007/s13337-012-0058-3

Sami Ullah, Muhammad Ali A Shah, Nosheen Riaz

{"title":"Recent Advances in Development of DNA Vaccines Against Hepatitis C virus.","authors":"Sami Ullah, Muhammad Ali A Shah, Nosheen Riaz","doi":"10.1007/s13337-012-0058-3","DOIUrl":"https://doi.org/10.1007/s13337-012-0058-3","url":null,"abstract":"Hepatitis C is one of the foremost challenging diseases all over the world. No vaccine has been developed, yet against Hepatitis C virus (HCV). This is partly due to the high mutation rate in the HCV genome, which generates new genotypes and sub genotypes. A mass of efforts have been devoted for the development of an efficient vaccine against HCV. DNA Vaccines, an emerging field of Vaccinology, grasp strong potential to be the most reliable and efficient mode of vaccination in the future. This technology is under investigation currently. Incredibly diverse approaches have been applied as an endeavor to develop a potent DNA vaccine against HCV. The HCV structural genes and the virus like particles have been attempted and so far the results are quite promising in the Lab animals. As there is no proper animal model for HCV infection except chimpanzees, it is very difficult to articulate whether these vaccines will also be pertinent in humans or not. This review will focus on different approaches being used for the development of DNA vaccines, the major tribulations in designing a DNA vaccine against HCV as well as the future prospects for the improvement of under trials DNA vaccines developed against HCV. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"23 3","pages":"253-60"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-012-0058-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31917656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Evaluation of rt-PCR assay for routine laboratory diagnosis of rabies in post mortem brain samples from different species of animals. rt-PCR法在不同动物死后脑标本狂犬病常规实验室诊断中的应用价值。

Indian Journal of Virology Pub Date : 2012-12-01 Epub Date: 2012-09-25 DOI: 10.1007/s13337-012-0109-9

R P Aravindh Babu, S Manoharan, P Ramadass, N D J Chandran

{"title":"Evaluation of rt-PCR assay for routine laboratory diagnosis of rabies in post mortem brain samples from different species of animals.","authors":"R P Aravindh Babu, S Manoharan, P Ramadass, N D J Chandran","doi":"10.1007/s13337-012-0109-9","DOIUrl":"https://doi.org/10.1007/s13337-012-0109-9","url":null,"abstract":"Rabies in domestic and wild animals continues to be a major public health threat in India. Rapid and accurate diagnosis of rabies in animals is therefore of utmost importance as the individuals who were in contact with the rabid animals are at a greater risk. A significant amount of diagnostic tissue samples submitted to our laboratory are often autolysed and the WHO recommended direct fluorescent antibody test (FAT) for rabies diagnosis cannot be used in such samples. In this pilot study we have evaluated three different diagnostic primer sets for rapid sensitive and specific detection of rabies genome from the brain samples of different species of animals. We have validated a sensitive RT-PCR assay using brain tissue samples from different species of animals such as cat, cattle, dog, mouse and human, for routine diagnosis of rabies. Our results show the potential of this assay as a confirmatory test when the FAT results are unreliable and also as an alternative diagnostic test in circumstances when the diagnostic samples are unsuitable for use in FAT. Furthermore the nucleotide sequence of nucleoprotein gene amplified using this assay can also be used for the molecular epidemiological study of the rabies viruses in India. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"23 3","pages":"392-6"},"PeriodicalIF":0.0,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-012-0109-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31916999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Interaction of Paramecium caudatum and Picornaviruses. 尾草履虫与小核糖核酸病毒的相互作用。

Indian Journal of Virology Pub Date : 2012-12-01 Epub Date: 2012-09-18 DOI: 10.1007/s13337-012-0100-5

Z A Karalyan, H E Voskanyan, N V Ramazyan, H S Zakaryan, E M Karalova

引用次数: 5