Indian Journal of Virology最新文献_第10页

White spot syndrome virus: Genotypes, Epidemiology and Evolutionary Studies. 白斑综合征病毒:基因型、流行病学和进化研究。

Indian Journal of Virology Pub Date : 2012-09-01 Epub Date: 2012-08-14 DOI: 10.1007/s13337-012-0078-z

Malathi Shekar, Balakrishnan Pradeep, Indrani Karunasagar

{"title":"White spot syndrome virus: Genotypes, Epidemiology and Evolutionary Studies.","authors":"Malathi Shekar, Balakrishnan Pradeep, Indrani Karunasagar","doi":"10.1007/s13337-012-0078-z","DOIUrl":"https://doi.org/10.1007/s13337-012-0078-z","url":null,"abstract":"White spot syndrome virus (WSSV) is a pathogen that has emerged globally affecting shrimp populations. Comparison of WSSV genome have shown the virus to share a high genetic similarity except for a few variable genomic loci that has been employed as markers in molecular epidemiology studies for determining the origin, evolution and spread in different geographical regions. Molecular genotyping of WSSV are based on genomic deletions associated with ORF23/24 and ORF14/15 variable regions and the three variable number of tandem repeat regions, ORF75, ORF94 and ORF125. Studies show the prevalence of several genotypes for WSSV with particular genotypes being more prevalent than others in a given geographical area. Deletions associated with ORF23/24 and ORF14/15 variable regions have proven to be of evolutionary significance. Fitness and virulence studies on different genotypes of WSSV suggest that all the strains of WSSV are equally virulent, but the one with smaller genomic size is the fittest. Studies also have shown that mixed genotype infection of WSSV correlates with lower disease outbreaks. This review focuses on the genotyping studies that were undertaken in elucidating WSSV evolution and epidemiology. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"23 2","pages":"175-83"},"PeriodicalIF":0.0,"publicationDate":"2012-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-012-0078-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31700560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Host and virus protein interaction studies in understanding shrimp virus gene function. 宿主与病毒蛋白相互作用在了解虾病毒基因功能中的研究。

Indian Journal of Virology Pub Date : 2012-09-01 Epub Date: 2012-08-14 DOI: 10.1007/s13337-012-0085-0

Subhendu Kumar Otta

{"title":"Host and virus protein interaction studies in understanding shrimp virus gene function.","authors":"Subhendu Kumar Otta","doi":"10.1007/s13337-012-0085-0","DOIUrl":"https://doi.org/10.1007/s13337-012-0085-0","url":null,"abstract":"Protein-protein interaction studies have been widely used in several fields to characterize an unknown protein. This in turn helps to find out several pathways to understand a complex mechanism or discover a drug for treatment. Among the methods, yeast two-hybrid has widely been used in human, animal and plant research studies. This aspect of research has also been found useful in understanding the shrimp virus gene function. With respect to White spot syndrome virus, interaction studies have been applied to elucidate virus structure, understand the mode of entry of the virus, mechanism of virus replication and also to discover some of the host anti-viral proteins. Interaction studies on other shrimp viruses are scanty and only few reports available on Yellow head virus and Taura syndrome virus. All these findings are still in preliminary stage and lot more studies are necessary to have the clear picture. Protein interaction research on other shrimp viruses are still lacking. Considering all these, it appears that this field of research has a wide scope to understand the virulence mechanism of shrimp viruses where very little information is available till date. ","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"23 2","pages":"184-90"},"PeriodicalIF":0.0,"publicationDate":"2012-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-012-0085-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31700561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Guest Editor's Note on the Special Issue of Indian Journal of Virology: Viruses of Cultured Aquatic Animals in the Asia-Pacific Region. 《印度病毒学杂志:亚太地区养殖水生动物的病毒》特刊客座编辑的注释。

Indian Journal of Virology Pub Date : 2012-09-01 DOI: 10.1007/s13337-012-0107-y

Indrani Karunasagar

引用次数: 1

RNA interference with special reference to combating viruses of crustacea. RNA干扰，特别是对付甲壳类病毒。

Indian Journal of Virology Pub Date : 2012-09-01 Epub Date: 2012-08-14 DOI: 10.1007/s13337-012-0084-1

Kathy La Fauce, Leigh Owens

引用次数: 19

Viruses associated with epizootic ulcerative syndrome: an update. 与兽疫性溃疡综合征相关的病毒:最新进展

Indian Journal of Virology Pub Date : 2012-09-01 Epub Date: 2012-09-19 DOI: 10.1007/s13337-012-0108-x

K Riji John, M Rosalind George

引用次数: 18

Emergence and reemergence of vaccinia-like viruses: global scenario and perspectives. 类疫苗病毒的出现和再次出现：全球情况和前景。

Indian Journal of Virology Pub Date : 2012-06-01 Epub Date: 2012-04-04 DOI: 10.1007/s13337-012-0068-1

R K Singh, V Balamurugan, V Bhanuprakash, G Venkatesan, M Hosamani

{"title":"Emergence and reemergence of vaccinia-like viruses: global scenario and perspectives.","authors":"R K Singh, V Balamurugan, V Bhanuprakash, G Venkatesan, M Hosamani","doi":"10.1007/s13337-012-0068-1","DOIUrl":"10.1007/s13337-012-0068-1","url":null,"abstract":"Among the members of the genus Orthopoxvirus (OPXV), vaccinia virus (VACV), the type species of the genus is a double-stranded DNA virus, belongs to the subfamily Chordopoxvirinae of the family Poxviridae. The causative agents of smallpox, VACV and Variola virus are mutually immunogenic and the type species of Orthopoxvirus, cause only mild complications in humans. Therefore, the VACV was used as a smallpox vaccine world over under mass immunization program promoted by World Health Organization, which lead to the variola eradication globally in 1979. Since then, no vaccination of human population has been carried out; however, vaccination has been continued for at-risk laboratory workers, military personnel and others working with recombinant VACV or other non-variola orthopoxviruses (OPXVs). There has now been a surge in the development of safer smallpox vaccines and understanding of the biology of VACV necessitating re-use of this vaccine in most vulnerable population, because of rise in bioterrorist threats globally. Also, globally there has been the emergence and re-emergence of vaccinia-like viruses (VLVs) in Brazil, buffalopox viruses in Egypt, Indonesia, India and its neighbouring countries like Nepal, Pakistan. Bioterrorism as well as emergence and re-emergence of the VLVs constitute a concern as 50 % of the population globally (40 % in USA) <30 years are unvaccinated and most vulnerable for smallpox reemergence. Thus, the search for new generation safer smallpox vaccine entails review of biology of VLVs in the smallpox-free world. In this review, we present occurrence of VLVs in the world with exhaustive discussion particularly on the emergence and re-emergence of these viruses in India and Brazil where VLVs are sufficiently studied.","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"23 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3550805/pdf/13337_2012_Article_68.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31567457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of major viral and sub viral pathogens incidence in apple orchards in himachal pradesh. 喜马偕尔邦苹果园主要病毒和亚病毒致病菌发病率的测定。

Indian Journal of Virology Pub Date : 2012-06-01 Epub Date: 2011-12-09 DOI: 10.1007/s13337-011-0056-x

Surender Kumar, Rahul Mohan Singh, Raja Ram, J Badyal, Vipin Hallan, A A Zaidi, Anupam Varma

{"title":"Determination of major viral and sub viral pathogens incidence in apple orchards in himachal pradesh.","authors":"Surender Kumar, Rahul Mohan Singh, Raja Ram, J Badyal, Vipin Hallan, A A Zaidi, Anupam Varma","doi":"10.1007/s13337-011-0056-x","DOIUrl":"https://doi.org/10.1007/s13337-011-0056-x","url":null,"abstract":"Apple is the major commercial horticulture crop in Himachal Pradesh and other hill states of Jammu & Kashmir, Uttarakhand and some parts of Northeastern states of India. In order to gather data on health status and incidence of virus and virus-like pathogens in apple orchards, survey was conducted in the month of June and September, 2010 in Hatkoti, Rohru, Kuthara, Jubbal and Khadapathar areas of major apple producing Shimla district of Himachal Pradesh. A total of 250 samples were collected and analyzed by DAS-ELISA, NASH and RT-PCR. NASH results indicated that a total of 117 samples were infected with Apple chlorotic leaf spot virus (ACLSV), Apple mosaic virus (ApMV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV) and Apple scar skin viroid (ASSVd). Results showed that ASSVd is predominant in these areas with highest infection rate of 27.6% followed by ASPV (17.2%), ACLSV (16.8%), ApMV (15.2%) and ASGV (12%). Mixed infection of these viruses and viroid was frequently detected in apple trees in Himachal Pradesh. The trees, which were positive for viruses and viroids, showed a variety of fruit deformation and rusting symptoms besides leaf deformation, mosaic and chlorosis.","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"23 1","pages":"75-9"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-011-0056-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31569015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Tomato leaf curl New Delhi virus is Associated With Pumpkin Leaf Curl: A New Disease in Northern India. 番茄卷叶病新德里病毒与南瓜卷叶病有关：印度北部的一种新病害

Indian Journal of Virology Pub Date : 2012-06-01 Epub Date: 2011-12-06 DOI: 10.1007/s13337-011-0054-z

Chigurupati Phaneendra, K R S S Rao, R K Jain, B Mandal

引用次数: 0

Evaluation of Reference Genes for the Relative Quantification of Apple stem grooving virus and Apple mosaic virus in Apple Trees. 苹果茎沟病毒和苹果花叶病毒相对定量内参基因的评价。

Indian Journal of Virology Pub Date : 2012-06-01 Epub Date: 2012-03-25 DOI: 10.1007/s13337-012-0065-4

S Gadiou, J K Kundu

引用次数: 14

Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis C virus. 环介导等温扩增法快速检测丙型肝炎病毒。

Indian Journal of Virology Pub Date : 2012-06-01 Epub Date: 2012-03-25 DOI: 10.1007/s13337-012-0067-2

Mohammad Kargar, Ahzam Askari, Abbas Doosti, Sadegh Ghorbani-Dalini

{"title":"Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Hepatitis C virus.","authors":"Mohammad Kargar, Ahzam Askari, Abbas Doosti, Sadegh Ghorbani-Dalini","doi":"10.1007/s13337-012-0067-2","DOIUrl":"https://doi.org/10.1007/s13337-012-0067-2","url":null,"abstract":"Hepatitis C virus (HCV) is a major public health problem and a leading cause of chronic liver disease. An estimated 180 million people are infected worldwide. In this study, we developed a loop-mediated isothermal amplification (LAMP) assay for rapid detection of HCV genomic RNA and compared the sensitivity of LAMP with nested-PCR. A total of 30 blood samples from HCV-infected patients were analyzed with six primers targeting conserved sequences of the HCV 5'UTR within 70 min, under isothermal conditions at 62 °C. Then, visualized by gel electrophoresis with ethidium bromide staining and detected by the naked-eye after adding SYBR Green I. All samples positive for HCV by nested PCR were confirmed by LAMP method. When visualized by gel electrophoresis and ethidium bromide staining, the HCV LAMP assay products appeared in a ladder pattern, with many bands of different sizes. The HCV LAMP product could also be detected by the naked-eye after adding SYBR Green I to the reaction tube and observing a color change from orange to green in positive samples. The HCV LAMP had the same sensitivity as a nested-PCR assay, the detection limit for the both systems were found to be 10 copies/mL of HCV RNA. The LAMP assay reported here is superior for rapid amplification, simple operation, and easy detection and will be useful for rapid and reliable clinical diagnosis of HCV in areas with limited resources, such as developing countries.","PeriodicalId":50370,"journal":{"name":"Indian Journal of Virology","volume":"23 1","pages":"18-23"},"PeriodicalIF":0.0,"publicationDate":"2012-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s13337-012-0067-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31567459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27