Evidence of Recombinant Citrus tristeza virus Isolate Occurring in Acid Lime cv. Pant Lemon Orchard in Uttarakhand Terai Region of Northern Himalaya in India.

Indian Journal of Virology Pub Date : 2013-06-01 Epub Date: 2012-12-18 DOI:10.1007/s13337-012-0118-8
Jaywant Kumar Singh, Avijit Tarafdar, Susheel Kumar Sharma, Kajal Kumar Biswas
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引用次数: 16

Abstract

The present study for the first time describes biological and molecular characterization of Citrus tristeza virus (CTV) occurring in the Terai area of Uttarakhand State in Northern Himalaya region of India. Direct antigen coated-ELISA and reverse transcriptase-polymerase chain reaction (RT-PCR) detected the CTV infection in Acid lime cv. Pant lemon (Citrus aurantifolia) orchards of Pantnagar with an estimated disease incidence of 16.6-20.5 %. To know the biological and genetic properties, an isolate, CTV Pant 4 was characterized. Isolate Pant 4 could be graft transmitted to Kinnow, Nagpur and Darjeeling mandarins, Mosambi sweet orange, Kagzi lime, Sweet lime, Sour orange but not to Rough lemon. The sequence analyses of the 5'ORF1a (3038 nucleotides) of LPro domain and 3'end (2058 nt) covering ORF7-ORF10 regions of the CTV genome revealed that Pant 4 was closely related to the previously reported Indian CTV isolate, Kpg3 from Northeastern Himalaya region with 97 and 98 % sequence identity, respectively. Whereas, it differed from the previously reported CTV isolate B165 from Southern India with 79 and 92 % identity, respectively for 5'ORF1a and 3' end regions. Recombination and SplitsTree decomposition analyses indicated that CTV isolate Pant 4 was a recombinant isolate originating from Kpg3 as a major and B165 as a minor donor.

重组柑橘tristeza病毒分离株在酸性石灰cv中发生的证据。印度北喜马拉雅的北阿坎德邦Terai地区的潘特柠檬果园。
本研究首次描述了发生在印度北喜马拉雅地区北阿坎德邦Terai地区的柑橘tristeza病毒(CTV)的生物学和分子特征。直接抗原包被法(elisa)和逆转录聚合酶链反应(RT-PCR)检测酸石灰cv感染情况。潘特纳格尔的潘特柠檬(Citrus aurantifolia)果园,估计发病率为16.6- 20.5%。为了解其生物学和遗传特性,对分离物CTV Pant 4进行了表征。分离菌株4号可以嫁接到金诺、那格浦尔和大吉岭柑桔、莫桑比甜橙、卡吉酸橙、甜橙、酸橙,但不能嫁接到粗柠檬上。对CTV基因组LPro域5′orf1a(3038个核苷酸)和覆盖ORF7-ORF10区域3′端(2058 nt)的序列分析表明,Pant 4与先前报道的印度CTV分离物Kpg3亲缘关系密切,序列同源性分别为97%和98%。然而,它与先前报道的来自印度南部的CTV分离物B165不同,在5'ORF1a和3'端区分别具有79%和92%的同源性。重组和splitree分解分析表明,CTV分离物pant4主要来源于Kpg3,次要来源于B165。
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来源期刊
Indian Journal of Virology
Indian Journal of Virology 医学-病毒学
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6-12 weeks
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