International Journal of Medical Microbiology最新文献_第8页

Heat shock protein 70 is involved in polaprezinc driven cell protection against Helicobacter pylori-induced injury 热休克蛋白70参与了polaprezinc驱动的细胞对幽门螺杆菌诱导的损伤的保护

IF 4.1 3区医学

International Journal of Medical Microbiology Pub Date : 2023-05-01 DOI: 10.1016/j.ijmm.2023.151582

Fansen Meng , Siying Zhu , Meiliang Gong , Hongjin Tao , Weihua Wang , Gangshi Wang

{"title":"Heat shock protein 70 is involved in polaprezinc driven cell protection against Helicobacter pylori-induced injury","authors":"Fansen Meng , Siying Zhu , Meiliang Gong , Hongjin Tao , Weihua Wang , Gangshi Wang","doi":"10.1016/j.ijmm.2023.151582","DOIUrl":"10.1016/j.ijmm.2023.151582","url":null,"abstract":"<div><p>Polaprezinc (PZ) plays a role in the protection of gastric mucosa and inhibiting <em>Helicobacter pylori</em> (<em>H. pylori</em>) growth in vitro. The objective of this study was to determine the protective effects of PZ on human gastric epithelial cells (GES-1) against <em>H. pylori</em>-induced damage, while also examining heat shock protein 70 (HSP70) as a potential underlying factor in this protection. Our findings revealed that PZ exerted bactericidal effects against <em>H. pylori</em> strains. We also observed that PZ mitigated the <em>H. pylori</em>-induced damage to GES-1 cells by increasing cell viability, reducing LDH release, and decreasing the secretion of pro-inflammatory factors such as MCP-1 and IL-6. Co-culturing PZ with GES-1 cells significantly up-regulated the GES-1 HSP70 expression in both a time and dose-dependent manner. Pre-incubating (for 12 h) or co-culturing (for 24 h) GES-1 cells with PZ reversed the down-regulation of HSP70 in GES-1 cells caused by <em>H. pylori</em> infection. However, when quercetin was used to inhibit the up-regulation of HSP70 in GES-1 cells, the protective effect of PZ on GES-1 cells was significantly reduced. Based on the results of this study, PZ exhibits a protective role on GES-1 cells against <em>H. pylori</em> injury, as well as a direct bactericidal effect on <em>H. pylori</em>. HSP70 is involved in the PZ-driven host cell protection against <em>H. pylori</em> injury. These findings provide insight into alternative strategies for <em>H. pylori</em> treatment.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 3","pages":"Article 151582"},"PeriodicalIF":4.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10176086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Longitudinal relationship between the gut microbiota variation and diversity and gut graft-versus-host disease (GVHD) following pediatric allogeneic hematopoietic cell transplantation (HCT) – Case series 儿童异基因造血细胞移植（HCT）后肠道微生物群变异和多样性与肠道移植物抗宿主病（GVHD）之间的纵向关系——病例系列

IF 4.1 3区医学

International Journal of Medical Microbiology Pub Date : 2023-05-01 DOI: 10.1016/j.ijmm.2023.151580

Ashley N. Gray , Nicole H. Tobin , Theodore B. Moore , Fan Li , Grace M. Aldrovandi

{"title":"Longitudinal relationship between the gut microbiota variation and diversity and gut graft-versus-host disease (GVHD) following pediatric allogeneic hematopoietic cell transplantation (HCT) – Case series","authors":"Ashley N. Gray , Nicole H. Tobin , Theodore B. Moore , Fan Li , Grace M. Aldrovandi","doi":"10.1016/j.ijmm.2023.151580","DOIUrl":"10.1016/j.ijmm.2023.151580","url":null,"abstract":"<div><p>Allogeneic Hematopoietic Cell Transplantation (HCT) offers children with life-threatening diseases a chance at survival. Complications from graft-versus-host disease (GVHD, Stages 0–4) represent a significant cause of morbidity and mortality which has been recently associated with gut dysbiosis the adult HCT population. Here, our objective was to conduct a prospective, longitudinal cohort study in nine pediatric allogeneic HCT participants by collecting longitudinally post-HCT stool specimens up to 1 year. Stool microbiota analyses showed that allogeneic HCT and antibiotic therapy lead to acute shifts in the diversity of the gut microbiota with those experiencing stages 3–4 gut GVHD having significantly greater microbiota variation over time when compared to control participants (p = 0.007). Pre-HCT microbiota diversity trended towards an inverse relationship with gut microbiota stability over time, however, this did not reach statistical significance (p = 0.05). Future large prospective studies are necessary to elucidate the mechanisms underlying these dynamic changes in the gut microbiota following pediatric allogeneic HCT.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 3","pages":"Article 151580"},"PeriodicalIF":4.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9649655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Plasmodium falciparum rhoptry neck protein 4 has conserved regions mediating interactions with receptors on human erythrocytes and hepatocyte membrane 恶性疟原虫杆状颈蛋白4具有介导与人红细胞和肝细胞膜受体相互作用的保守区

IF 4.1 3区医学

International Journal of Medical Microbiology Pub Date : 2023-05-01 DOI: 10.1016/j.ijmm.2023.151579

Fredy A. Pulido-Quevedo , Gabriela Arévalo-Pinzón , Jeimmy J. Castañeda-Ramírez , Adriana Barreto-Santamaría , Manuel E. Patarroyo , Manuel A. Patarroyo

{"title":"Plasmodium falciparum rhoptry neck protein 4 has conserved regions mediating interactions with receptors on human erythrocytes and hepatocyte membrane","authors":"Fredy A. Pulido-Quevedo , Gabriela Arévalo-Pinzón , Jeimmy J. Castañeda-Ramírez , Adriana Barreto-Santamaría , Manuel E. Patarroyo , Manuel A. Patarroyo","doi":"10.1016/j.ijmm.2023.151579","DOIUrl":"10.1016/j.ijmm.2023.151579","url":null,"abstract":"<div><p><em>Plasmodium falciparum</em>-related malaria represents a serious worldwide public health problem due to its high mortality rates. <em>P. falciparum</em> expresses rhoptry neck protein 4 (<em>Pf</em>RON4) in merozoite and sporozoite rhoptries, it participates in tight junction-TJ formation via the AMA-1/RON complex and is refractory to complete genetic deletion. Despite this, which <em>Pf</em>RON4 key regions interact with host cells remain unknown; such information would be useful for combating falciparum malaria. Thirty-two RON4 conserved region-derived peptides were chemically synthesised for determining and characterising <em>Pf</em>RON4 regions having high host cell binding affinity (high activity binding peptides or HABPs). Receptor-ligand interaction/binding assays determined their specific binding capability, the nature of their receptors and their ability to inhibit in vitro parasite invasion. Peptides 42477, 42479, 42480, 42505 and 42513 had greater than 2% erythrocyte binding activity, whilst peptides 42477 and 42480 specifically bound to HepG2 membrane, both of them having micromolar and submicromolar range dissociation constants (<em>Kd)</em>. Cell-peptide interaction was sensitive to treating erythrocytes with trypsin and/or chymotrypsin and HepG2 with heparinase I and chondroitinase ABC, suggesting protein-type (erythrocyte) and heparin and/or chondroitin sulphate proteoglycan receptors (HepG2) for <em>Pf</em>RON4. Erythrocyte invasion inhibition assays confirmed HABPs’ importance during merozoite invasion. <em>Pf</em>RON4 800–819 (42477) and 860–879 (42480) regions specifically interacted with host cells, thereby supporting their inclusion in a subunit-based, multi-antigen, multistage anti-malarial vaccine.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 3","pages":"Article 151579"},"PeriodicalIF":4.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9640718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preclinical evaluation of 2-[18F]fluorodeoxysorbitol as a tracer for targeted imaging of Enterobacterales infection 2-[18F]氟脱氧糖苷醇作为肠杆菌感染靶向显像的临床前评价

IF 4.1 3区医学

International Journal of Medical Microbiology Pub Date : 2023-05-01 DOI: 10.1016/j.ijmm.2023.151581

Lisanne M. Braams , Jürgen W.A. Sijbesma , Hendrikus H. Boersma , Jan Maarten van Dijl , Philip H. Elsinga , Andor W.J.M. Glaudemans , Riemer H.J.A. Slart , Marleen van Oosten

{"title":"Preclinical evaluation of 2-[18F]fluorodeoxysorbitol as a tracer for targeted imaging of Enterobacterales infection","authors":"Lisanne M. Braams , Jürgen W.A. Sijbesma , Hendrikus H. Boersma , Jan Maarten van Dijl , Philip H. Elsinga , Andor W.J.M. Glaudemans , Riemer H.J.A. Slart , Marleen van Oosten","doi":"10.1016/j.ijmm.2023.151581","DOIUrl":"https://doi.org/10.1016/j.ijmm.2023.151581","url":null,"abstract":"<div><p>Fluorine-18-fluorodeoxyglucose ([<sup>18</sup>F]FDG) positron emission tomography (<sup>18</sup>F-FDG-PET) is widely used for the detection of inflammatory and infectious diseases. Although this modality has proven to be a useful diagnostic tool, reliable distinction of bacterial infection from sterile inflammation or even from a malignancy remains challenging. Therefore, there is a need for bacteria-specific tracers for PET imaging that facilitate a reliable distinction of bacterial infection from other pathology. The present study was aimed at exploring the potential of 2-[<sup>18</sup>F]-fluorodeoxysorbitol ([<sup>18</sup>F]FDS) as a tracer for detection of <em>Enterobacterales</em> infections. Sorbitol is a sugar alcohol that is commonly metabolized by bacteria of the <em>Enterobacterales</em> order, but not by mammalian cells, which makes it an attractive candidate for targeted bacterial imaging. The latter is important in view of the serious clinical implications of infections caused by <em>Enterobacterales.</em> Here we demonstrate that sorbitol-based PET can be applied to detect a broad range of clinical bacterial isolates not only in vitro, but also in blood and ascites samples from patients suffering from <em>Enterobacterales</em> infections. Notably, the possible application of [<sup>18</sup>F]FDS is not limited to <em>Enterobacterales</em> since <em>Pseudomonas aeruginosa</em> and <em>Corynebacterium jeikeium</em> also showed substantial uptake of this tracer. We conclude that [<sup>18</sup>F]FDS is a promising tracer for PET-imaging of infections caused by a group of bacteria that can cause serious invasive disease.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 3","pages":"Article 151581"},"PeriodicalIF":4.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49773813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of folate metabolism-related substances affecting Staphylococcus aureus infection 叶酸代谢相关物质影响金黄色葡萄球菌感染的机制

IF 4.1 3区医学

International Journal of Medical Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.ijmm.2023.151577

Qiyuan Jin , Xiaolu Xie , Yaxuan Zhai, Haifang Zhang

{"title":"Mechanisms of folate metabolism-related substances affecting Staphylococcus aureus infection","authors":"Qiyuan Jin , Xiaolu Xie , Yaxuan Zhai, Haifang Zhang","doi":"10.1016/j.ijmm.2023.151577","DOIUrl":"10.1016/j.ijmm.2023.151577","url":null,"abstract":"<div><p><em>Staphylococcus aureus</em> (<em>S. aureus</em>) is one of the critical clinical pathogens which can cause multiple diseases ranging from skin infections to fatal sepsis. <em>S. aureus</em> is generally considered to be an extracellular pathogen. However, more and more evidence has shown that <em>S. aureus</em> can survive inside various cells. Folate plays an essential role in multiple life activities, including the conversion of serine and glycine, the remethylation of homocysteine to methionine, and the <em>de novo</em> synthesis of purine /dTMP, et al. More and more studies reported that <em>S. aureus</em> intracellular infection requires the involvement of folate metabolism. This review focused on the mechanisms of folate metabolism and related substances affecting <em>S. aureus</em> infection. Loss of tetrahydrofolic acid (THF)-dependent dTMP directly inhibits the nucleotide synthesis pathway of the <em>S. aureus</em> due to <em>pabA</em> deficiency. Besides, trimethoprim-sulfamethoxazole (TMP/SMX), a potent antibiotic that treats <em>S. aureus</em> infections, interferes in the process of the folate mechanism and leads to the production of thymidine-dependent small-colony variants (TD-SCVs). In addition, <em>S. aureus</em> is resistant to lysostaphin in the presence of serine hydroxymethyltransferase (SHMT). We provide new insights for understanding the molecular pathogenesis of <em>S. aureus</em> infection.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 2","pages":"Article 151577"},"PeriodicalIF":4.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9261522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Pretreatment with 3-methyladenine ameliorated Pseudomonas aeruginosa-induced acute pneumonia by inhibiting cell death of neutrophils in a mouse infection model 3-甲基腺嘌呤预处理通过抑制小鼠感染模型中性粒细胞的死亡改善铜绿假单胞菌诱导的急性肺炎

IF 4.1 3区医学

International Journal of Medical Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.ijmm.2023.151574

Lei Yue , Han Cao , Jialong Qi , Jin Yuan , Xin Wang , Yunfei Wang , Bin Shan , Huaxin Ke , Hua Li , Ning Luan , Cunbao Liu

{"title":"Pretreatment with 3-methyladenine ameliorated Pseudomonas aeruginosa-induced acute pneumonia by inhibiting cell death of neutrophils in a mouse infection model","authors":"Lei Yue , Han Cao , Jialong Qi , Jin Yuan , Xin Wang , Yunfei Wang , Bin Shan , Huaxin Ke , Hua Li , Ning Luan , Cunbao Liu","doi":"10.1016/j.ijmm.2023.151574","DOIUrl":"10.1016/j.ijmm.2023.151574","url":null,"abstract":"<div><p><em>Pseudomonas aeruginosa</em> is one of the leading causes of nosocomial infections worldwide. Clinical isolates that are resistant to multiple antimicrobials make it intractable. The interactions between <em>P. aeruginosa</em> and host cell death have multiple effects on bacterial clearance and inflammation; however, the potential intervention effects remain to be defined. Herein, we demonstrated that intravenous administration of 3-methyladenine before, but not after, <em>P. aeruginosa</em> infection enhanced autophagy-independent survival, which was accompanied by a decrease in the bacterial load, alleviation of pathology and reduction in inflammatory cytokines, in an acute pneumonia mouse model. Interestingly, these beneficial effects were not dependent on neutrophil recruitment or phagocytosis, but on the enhanced killing capacity induced by inhibiting the cell death of 3-MA pretreated neutrophils. These findings demonstrate a novel protective role of 3-MA pretreatment in <em>P. aeruginosa</em>-induced acute pneumonia.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 2","pages":"Article 151574"},"PeriodicalIF":4.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9321422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial and antibiofilm efficacy of repurposing drug hexestrol against methicillin-resistant Staphylococcus aureus 己炔雌醇对耐甲氧西林金黄色葡萄球菌的抗菌和抗生物膜作用

IF 4.1 3区医学

International Journal of Medical Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.ijmm.2023.151578

Shasha Liu , Pengfei She , Zehao Li , Yimin Li , Linhui Li , Yifan Yang , Linying Zhou , Yong Wu

{"title":"Antibacterial and antibiofilm efficacy of repurposing drug hexestrol against methicillin-resistant Staphylococcus aureus","authors":"Shasha Liu , Pengfei She , Zehao Li , Yimin Li , Linhui Li , Yifan Yang , Linying Zhou , Yong Wu","doi":"10.1016/j.ijmm.2023.151578","DOIUrl":"10.1016/j.ijmm.2023.151578","url":null,"abstract":"<div><p>There has been an explosion in the prevalence of methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) because of the indiscriminate use of antibiotics. In this study, we repurposed hexestrol (HXS) as an antibacterial agent to fight planktonic and biofilm-related MRSA infections. HXS is a nonsteroidal synthetic estrogen that targets estrogen receptors (ERα and ERβ) and has been used as a hormonal antineoplastic agent. In our work, the minimum inhibitory concentrations (MICs) were determined using the antimicrobial susceptibility of MSSA and MRSA strains. Anti-biofilm activity was evaluated using biofilm inhibition and eradication assays. Biofilm-related genes were analyzed with or without HXS treatment using RT<img>qPCR analysis of <em>S. aureus</em>. HXS was tested using the checkerboard dilution assay to identify antibiotics that may have synergistic effects. Measurement of ATP and detection of ATPase allowed the determination of bacterial energy metabolism. As shown in the results, HXS showed effective antimicrobial activity against <em>S. aureus</em>, including both type strains and clinical isolations, with MICs of 16 µg/mL. Sub-HXS strongly inhibited the adhesion of <em>S. aureus</em>. The content of extracellular polymeric substances (EPS) and the relative transcription levels of <em>eno</em>, <em>sacC</em>, <em>clfA</em>, <em>pls</em> and <em>fnbpB</em> were reduced after HXS treatment. HXS showed antibacterial effects against <em>S. aureus</em> and synergistic activity with aminoglycosides by directly interfering with cellular energy metabolism. HXS inhibits adhesion and biofilm formation and eradicates biofilms formed by MRSA by reducing the expression of related genes. Furthermore, HXS increases the susceptibility of aminoglycosides against MRSA. In conclusion, HXS is a repurposed drug that may be a promising therapeutic option for MRSA infection.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 2","pages":"Article 151578"},"PeriodicalIF":4.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9624415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does pre-incubation in selective-enrichment media improve the detection of diarrheagenic Escherichia coli using the RIDA®GENE PCR? 选择性富集培养基中的预孵育是否能提高RIDA®基因PCR对致泻性大肠杆菌的检测?

IF 4.1 3区医学

International Journal of Medical Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.ijmm.2023.151575

Neele J. Froböse , Ioana D. Olaru , Julia Sophie Schneider , Wenlan Zhang , Alexander Mellmann , Franziska Schuler , Tobias Grebe , Frieder Schaumburg

引用次数: 0

Anti-inflammatory effect of chlorogenic acid in Klebsiella pneumoniae-induced pneumonia by inactivating the p38MAPK pathway 绿原酸通过失活p38MAPK途径对肺炎克雷伯菌肺炎的抗炎作用

IF 4.1 3区医学

International Journal of Medical Microbiology Pub Date : 2023-03-01 DOI: 10.1016/j.ijmm.2023.151576

Yizhe Zhang , Chaoyin Zhu , Hongjun Zhao , Zhanyang Sun , Xiaodi Wang

{"title":"Anti-inflammatory effect of chlorogenic acid in Klebsiella pneumoniae-induced pneumonia by inactivating the p38MAPK pathway","authors":"Yizhe Zhang , Chaoyin Zhu , Hongjun Zhao , Zhanyang Sun , Xiaodi Wang","doi":"10.1016/j.ijmm.2023.151576","DOIUrl":"10.1016/j.ijmm.2023.151576","url":null,"abstract":"<div><h3>Introduction</h3><p>Pneumonia is an inflammation-related respiratory infection and chlorogenic acid (CGA) possesses a wide variety of bioactive properties, such as anti-inflammation and anti-bacteria.</p></div><div><h3>Aim</h3><p>This study explored the anti-inflammatory mechanism of CGA in <em>Klebsiella pneumoniae</em> (Kp)-induced rats with severe pneumonia.</p></div><div><h3>Methods</h3><p>The pneumonia rat models were established by infection with Kp and treated with CGA. Survival rates, bacterial load, lung water content, and cell numbers in the bronchoalveolar lavage fluid were recorded, lung pathological changes were scored, and levels of inflammatory cytokines were determined by enzyme-linked immunosorbent assay. RLE6TN cells were infected with Kp and treated with CGA. The expression levels of microRNA (miR)-124–3p, p38, and mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MK2) in lung tissues and RLE6TN cells were quantified by real-time quantitative polymerase chain reaction or Western blotting. The binding of miR-124–3p to p38 was validated by the dual-luciferase and RNA pull-down assays. <em>In vitro</em>, the functional rescue experiments were performed using miR-124–3p inhibitor or p38 agonist.</p></div><div><h3>Results</h3><p>Kp-induced pneumonia rats presented high mortality, increased lung inflammatory infiltration and the release of inflammatory cytokines, and enhanced bacterial load, while CGA treatment improved rat survival rates and the above situations. CGA increased miR-124–3p expression, and miR-124–3p inhibited p38 expression and inactivated the p38MAPK pathway. Inhibition of miR-124–3p or activation of the p38MAPK pathway reversed the alleviative effect of CGA on pneumonia in vitro.</p></div><div><h3>Conclusion</h3><p>CGA upregulated miR-124–3p expression and inactivated the p38MAPK pathway to downregulate inflammatory levels, facilitating the recovery of Kp-induced pneumonia rats.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 2","pages":"Article 151576"},"PeriodicalIF":4.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9273510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Comparative analysis of outer membrane vesicles from uropathogenic Escherichia coli reveal the role of aromatic amino acids synthesis proteins in motility 对尿路致病性大肠杆菌外膜囊泡的比较分析揭示了芳香氨基酸合成蛋白在运动中的作用

IF 4.1 3区医学

International Journal of Medical Microbiology Pub Date : 2023-01-01 DOI: 10.1016/j.ijmm.2023.151573

LiangZhe Liu , Carmen Oi Kwan Law , Qichang Nie , Hoa Quynh Pham , Haiying Ma , Liang Zhang , Pak Leung Ho , Terrence Chi-Kong Lau

{"title":"Comparative analysis of outer membrane vesicles from uropathogenic Escherichia coli reveal the role of aromatic amino acids synthesis proteins in motility","authors":"LiangZhe Liu , Carmen Oi Kwan Law , Qichang Nie , Hoa Quynh Pham , Haiying Ma , Liang Zhang , Pak Leung Ho , Terrence Chi-Kong Lau","doi":"10.1016/j.ijmm.2023.151573","DOIUrl":"10.1016/j.ijmm.2023.151573","url":null,"abstract":"<div><p>Uropathogenic <em>Escherichia coli</em> (UPEC) are causative agent that causes urinary tract infections (UTIs) and the recent emergence of multidrug resistance (MDR) of UPEC increases the burden on the community. Recent studies of bacterial outer membrane vesicles (OMV) identified various factors including proteins, nucleic acids, and small molecules which provided inter-cellular communication within the bacterial population. However, the components of UPEC-specific OMVs and their functional role remain unclear. Here, we systematically determined the proteomes of UPEC-OMVs and identified the specific components that provide functions to the recipient bacteria. Based on the functional network of OMVs’ proteomes, a group of signaling peptides was found in all OMVs which provide communication among bacteria. Moreover, we demonstrated that treatment with UPEC-OMVs affected the motility and biofilm formation of the recipient bacteria, and further identified aromatic amino acid (AAA) biosynthesis proteins as the key factors to provide their movement.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 1","pages":"Article 151573"},"PeriodicalIF":4.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10660081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2