International Journal of Medical Microbiology最新文献

{"title":"Plasmodium falciparum rhoptry neck protein 4 has conserved regions mediating interactions with receptors on human erythrocytes and hepatocyte membrane","authors":"Fredy A. Pulido-Quevedo ,&nbsp;Gabriela Arévalo-Pinzón ,&nbsp;Jeimmy J. Castañeda-Ramírez ,&nbsp;Adriana Barreto-Santamaría ,&nbsp;Manuel E. Patarroyo ,&nbsp;Manuel A. Patarroyo","doi":"10.1016/j.ijmm.2023.151579","DOIUrl":"10.1016/j.ijmm.2023.151579","url":null,"abstract":"<div><p><em>Plasmodium falciparum</em>-related malaria represents a serious worldwide public health problem due to its high mortality rates. <em>P. falciparum</em> expresses rhoptry neck protein 4 (<em>Pf</em>RON4) in merozoite and sporozoite rhoptries, it participates in tight junction-TJ formation via the AMA-1/RON complex and is refractory to complete genetic deletion. Despite this, which <em>Pf</em>RON4 key regions interact with host cells remain unknown; such information would be useful for combating falciparum malaria. Thirty-two RON4 conserved region-derived peptides were chemically synthesised for determining and characterising <em>Pf</em>RON4 regions having high host cell binding affinity (high activity binding peptides or HABPs). Receptor-ligand interaction/binding assays determined their specific binding capability, the nature of their receptors and their ability to inhibit in vitro parasite invasion. Peptides 42477, 42479, 42480, 42505 and 42513 had greater than 2% erythrocyte binding activity, whilst peptides 42477 and 42480 specifically bound to HepG2 membrane, both of them having micromolar and submicromolar range dissociation constants (<em>Kd)</em>. Cell-peptide interaction was sensitive to treating erythrocytes with trypsin and/or chymotrypsin and HepG2 with heparinase I and chondroitinase ABC, suggesting protein-type (erythrocyte) and heparin and/or chondroitin sulphate proteoglycan receptors (HepG2) for <em>Pf</em>RON4. Erythrocyte invasion inhibition assays confirmed HABPs’ importance during merozoite invasion. <em>Pf</em>RON4 800–819 (42477) and 860–879 (42480) regions specifically interacted with host cells, thereby supporting their inclusion in a subunit-based, multi-antigen, multistage anti-malarial vaccine.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 3","pages":"Article 151579"},"PeriodicalIF":4.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9640718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preclinical evaluation of 2-[18F]fluorodeoxysorbitol as a tracer for targeted imaging of Enterobacterales infection","authors":"Lisanne M. Braams ,&nbsp;Jürgen W.A. Sijbesma ,&nbsp;Hendrikus H. Boersma ,&nbsp;Jan Maarten van Dijl ,&nbsp;Philip H. Elsinga ,&nbsp;Andor W.J.M. Glaudemans ,&nbsp;Riemer H.J.A. Slart ,&nbsp;Marleen van Oosten","doi":"10.1016/j.ijmm.2023.151581","DOIUrl":"https://doi.org/10.1016/j.ijmm.2023.151581","url":null,"abstract":"<div><p>Fluorine-18-fluorodeoxyglucose ([¹⁸F]FDG) positron emission tomography (¹⁸F-FDG-PET) is widely used for the detection of inflammatory and infectious diseases. Although this modality has proven to be a useful diagnostic tool, reliable distinction of bacterial infection from sterile inflammation or even from a malignancy remains challenging. Therefore, there is a need for bacteria-specific tracers for PET imaging that facilitate a reliable distinction of bacterial infection from other pathology. The present study was aimed at exploring the potential of 2-[¹⁸F]-fluorodeoxysorbitol ([¹⁸F]FDS) as a tracer for detection of <em>Enterobacterales</em> infections. Sorbitol is a sugar alcohol that is commonly metabolized by bacteria of the <em>Enterobacterales</em> order, but not by mammalian cells, which makes it an attractive candidate for targeted bacterial imaging. The latter is important in view of the serious clinical implications of infections caused by <em>Enterobacterales.</em> Here we demonstrate that sorbitol-based PET can be applied to detect a broad range of clinical bacterial isolates not only in vitro, but also in blood and ascites samples from patients suffering from <em>Enterobacterales</em> infections. Notably, the possible application of [¹⁸F]FDS is not limited to <em>Enterobacterales</em> since <em>Pseudomonas aeruginosa</em> and <em>Corynebacterium jeikeium</em> also showed substantial uptake of this tracer. We conclude that [¹⁸F]FDS is a promising tracer for PET-imaging of infections caused by a group of bacteria that can cause serious invasive disease.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 3","pages":"Article 151581"},"PeriodicalIF":4.1,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49773813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of folate metabolism-related substances affecting Staphylococcus aureus infection","authors":"Qiyuan Jin ,&nbsp;Xiaolu Xie ,&nbsp;Yaxuan Zhai,&nbsp;Haifang Zhang","doi":"10.1016/j.ijmm.2023.151577","DOIUrl":"10.1016/j.ijmm.2023.151577","url":null,"abstract":"<div><p><em>Staphylococcus aureus</em> (<em>S. aureus</em>) is one of the critical clinical pathogens which can cause multiple diseases ranging from skin infections to fatal sepsis. <em>S. aureus</em> is generally considered to be an extracellular pathogen. However, more and more evidence has shown that <em>S. aureus</em> can survive inside various cells. Folate plays an essential role in multiple life activities, including the conversion of serine and glycine, the remethylation of homocysteine to methionine, and the <em>de novo</em> synthesis of purine /dTMP, et al. More and more studies reported that <em>S. aureus</em> intracellular infection requires the involvement of folate metabolism. This review focused on the mechanisms of folate metabolism and related substances affecting <em>S. aureus</em> infection. Loss of tetrahydrofolic acid (THF)-dependent dTMP directly inhibits the nucleotide synthesis pathway of the <em>S. aureus</em> due to <em>pabA</em> deficiency. Besides, trimethoprim-sulfamethoxazole (TMP/SMX), a potent antibiotic that treats <em>S. aureus</em> infections, interferes in the process of the folate mechanism and leads to the production of thymidine-dependent small-colony variants (TD-SCVs). In addition, <em>S. aureus</em> is resistant to lysostaphin in the presence of serine hydroxymethyltransferase (SHMT). We provide new insights for understanding the molecular pathogenesis of <em>S. aureus</em> infection.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 2","pages":"Article 151577"},"PeriodicalIF":4.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9261522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pretreatment with 3-methyladenine ameliorated Pseudomonas aeruginosa-induced acute pneumonia by inhibiting cell death of neutrophils in a mouse infection model","authors":"Lei Yue ,&nbsp;Han Cao ,&nbsp;Jialong Qi ,&nbsp;Jin Yuan ,&nbsp;Xin Wang ,&nbsp;Yunfei Wang ,&nbsp;Bin Shan ,&nbsp;Huaxin Ke ,&nbsp;Hua Li ,&nbsp;Ning Luan ,&nbsp;Cunbao Liu","doi":"10.1016/j.ijmm.2023.151574","DOIUrl":"10.1016/j.ijmm.2023.151574","url":null,"abstract":"<div><p><em>Pseudomonas aeruginosa</em> is one of the leading causes of nosocomial infections worldwide. Clinical isolates that are resistant to multiple antimicrobials make it intractable. The interactions between <em>P. aeruginosa</em> and host cell death have multiple effects on bacterial clearance and inflammation; however, the potential intervention effects remain to be defined. Herein, we demonstrated that intravenous administration of 3-methyladenine before, but not after, <em>P. aeruginosa</em> infection enhanced autophagy-independent survival, which was accompanied by a decrease in the bacterial load, alleviation of pathology and reduction in inflammatory cytokines, in an acute pneumonia mouse model. Interestingly, these beneficial effects were not dependent on neutrophil recruitment or phagocytosis, but on the enhanced killing capacity induced by inhibiting the cell death of 3-MA pretreated neutrophils. These findings demonstrate a novel protective role of 3-MA pretreatment in <em>P. aeruginosa</em>-induced acute pneumonia.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 2","pages":"Article 151574"},"PeriodicalIF":4.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9321422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and antibiofilm efficacy of repurposing drug hexestrol against methicillin-resistant Staphylococcus aureus","authors":"Shasha Liu ,&nbsp;Pengfei She ,&nbsp;Zehao Li ,&nbsp;Yimin Li ,&nbsp;Linhui Li ,&nbsp;Yifan Yang ,&nbsp;Linying Zhou ,&nbsp;Yong Wu","doi":"10.1016/j.ijmm.2023.151578","DOIUrl":"10.1016/j.ijmm.2023.151578","url":null,"abstract":"<div><p>There has been an explosion in the prevalence of methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) because of the indiscriminate use of antibiotics. In this study, we repurposed hexestrol (HXS) as an antibacterial agent to fight planktonic and biofilm-related MRSA infections. HXS is a nonsteroidal synthetic estrogen that targets estrogen receptors (ERα and ERβ) and has been used as a hormonal antineoplastic agent. In our work, the minimum inhibitory concentrations (MICs) were determined using the antimicrobial susceptibility of MSSA and MRSA strains. Anti-biofilm activity was evaluated using biofilm inhibition and eradication assays. Biofilm-related genes were analyzed with or without HXS treatment using RT<img>qPCR analysis of <em>S. aureus</em>. HXS was tested using the checkerboard dilution assay to identify antibiotics that may have synergistic effects. Measurement of ATP and detection of ATPase allowed the determination of bacterial energy metabolism. As shown in the results, HXS showed effective antimicrobial activity against <em>S. aureus</em>, including both type strains and clinical isolations, with MICs of 16 µg/mL. Sub-HXS strongly inhibited the adhesion of <em>S. aureus</em>. The content of extracellular polymeric substances (EPS) and the relative transcription levels of <em>eno</em>, <em>sacC</em>, <em>clfA</em>, <em>pls</em> and <em>fnbpB</em> were reduced after HXS treatment. HXS showed antibacterial effects against <em>S. aureus</em> and synergistic activity with aminoglycosides by directly interfering with cellular energy metabolism. HXS inhibits adhesion and biofilm formation and eradicates biofilms formed by MRSA by reducing the expression of related genes. Furthermore, HXS increases the susceptibility of aminoglycosides against MRSA. In conclusion, HXS is a repurposed drug that may be a promising therapeutic option for MRSA infection.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 2","pages":"Article 151578"},"PeriodicalIF":4.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9624415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does pre-incubation in selective-enrichment media improve the detection of diarrheagenic Escherichia coli using the RIDA®GENE PCR?","authors":"Neele J. Froböse ,&nbsp;Ioana D. Olaru ,&nbsp;Julia Sophie Schneider ,&nbsp;Wenlan Zhang ,&nbsp;Alexander Mellmann ,&nbsp;Franziska Schuler ,&nbsp;Tobias Grebe ,&nbsp;Frieder Schaumburg","doi":"10.1016/j.ijmm.2023.151575","DOIUrl":"10.1016/j.ijmm.2023.151575","url":null,"abstract":"<div><p>We aimed to investigate whether a selective pre-PCR enrichment step improves test performance of RIDA®GENE EHEC/EPEC to detect diarrheagenic <em>Escherichia coli</em> from stool samples. Each of the 250 stool samples was analyzed for the presence of <em>stx1/2</em> and <em>eae</em> both with and without pre-PCR enrichment in selective broth. In comparison to a reference method, sensitivities for <em>stx1/2</em> and <em>eae</em> with and without pre-PCR enrichment were 84% (95%CI 70–93) and 89% (<em>stx</em>1/2, 95%CI 76–96), and 71% (95%CI 58–81) and 72% (<em>eae</em>, 95%CI 60–82), respectively. Specificity exceeded 97% for both methods and target genes. In summary, pre-PCR broth enrichment did not improve test performance.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 2","pages":"Article 151575"},"PeriodicalIF":4.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9621933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory effect of chlorogenic acid in Klebsiella pneumoniae-induced pneumonia by inactivating the p38MAPK pathway","authors":"Yizhe Zhang ,&nbsp;Chaoyin Zhu ,&nbsp;Hongjun Zhao ,&nbsp;Zhanyang Sun ,&nbsp;Xiaodi Wang","doi":"10.1016/j.ijmm.2023.151576","DOIUrl":"10.1016/j.ijmm.2023.151576","url":null,"abstract":"<div><h3>Introduction</h3><p>Pneumonia is an inflammation-related respiratory infection and chlorogenic acid (CGA) possesses a wide variety of bioactive properties, such as anti-inflammation and anti-bacteria.</p></div><div><h3>Aim</h3><p>This study explored the anti-inflammatory mechanism of CGA in <em>Klebsiella pneumoniae</em> (Kp)-induced rats with severe pneumonia.</p></div><div><h3>Methods</h3><p>The pneumonia rat models were established by infection with Kp and treated with CGA. Survival rates, bacterial load, lung water content, and cell numbers in the bronchoalveolar lavage fluid were recorded, lung pathological changes were scored, and levels of inflammatory cytokines were determined by enzyme-linked immunosorbent assay. RLE6TN cells were infected with Kp and treated with CGA. The expression levels of microRNA (miR)-124–3p, p38, and mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MK2) in lung tissues and RLE6TN cells were quantified by real-time quantitative polymerase chain reaction or Western blotting. The binding of miR-124–3p to p38 was validated by the dual-luciferase and RNA pull-down assays. <em>In vitro</em>, the functional rescue experiments were performed using miR-124–3p inhibitor or p38 agonist.</p></div><div><h3>Results</h3><p>Kp-induced pneumonia rats presented high mortality, increased lung inflammatory infiltration and the release of inflammatory cytokines, and enhanced bacterial load, while CGA treatment improved rat survival rates and the above situations. CGA increased miR-124–3p expression, and miR-124–3p inhibited p38 expression and inactivated the p38MAPK pathway. Inhibition of miR-124–3p or activation of the p38MAPK pathway reversed the alleviative effect of CGA on pneumonia in vitro.</p></div><div><h3>Conclusion</h3><p>CGA upregulated miR-124–3p expression and inactivated the p38MAPK pathway to downregulate inflammatory levels, facilitating the recovery of Kp-induced pneumonia rats.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 2","pages":"Article 151576"},"PeriodicalIF":4.1,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9273510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of outer membrane vesicles from uropathogenic Escherichia coli reveal the role of aromatic amino acids synthesis proteins in motility","authors":"LiangZhe Liu ,&nbsp;Carmen Oi Kwan Law ,&nbsp;Qichang Nie ,&nbsp;Hoa Quynh Pham ,&nbsp;Haiying Ma ,&nbsp;Liang Zhang ,&nbsp;Pak Leung Ho ,&nbsp;Terrence Chi-Kong Lau","doi":"10.1016/j.ijmm.2023.151573","DOIUrl":"10.1016/j.ijmm.2023.151573","url":null,"abstract":"<div><p>Uropathogenic <em>Escherichia coli</em> (UPEC) are causative agent that causes urinary tract infections (UTIs) and the recent emergence of multidrug resistance (MDR) of UPEC increases the burden on the community. Recent studies of bacterial outer membrane vesicles (OMV) identified various factors including proteins, nucleic acids, and small molecules which provided inter-cellular communication within the bacterial population. However, the components of UPEC-specific OMVs and their functional role remain unclear. Here, we systematically determined the proteomes of UPEC-OMVs and identified the specific components that provide functions to the recipient bacteria. Based on the functional network of OMVs’ proteomes, a group of signaling peptides was found in all OMVs which provide communication among bacteria. Moreover, we demonstrated that treatment with UPEC-OMVs affected the motility and biofilm formation of the recipient bacteria, and further identified aromatic amino acid (AAA) biosynthesis proteins as the key factors to provide their movement.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"313 1","pages":"Article 151573"},"PeriodicalIF":4.1,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10660081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanopore 16S amplicon sequencing enables rapid detection of pathogen in knee periprosthetic joint infection","authors":"Hyuk-Soo Han ,&nbsp;Du Hyun Ro ,&nbsp;Jeehyeok Chung ,&nbsp;Narae Kim ,&nbsp;Jangsup Moon","doi":"10.1016/j.ijmm.2022.151570","DOIUrl":"10.1016/j.ijmm.2022.151570","url":null,"abstract":"<div><h3>Objectives</h3><p>We investigated whether nanopore 16S amplicon sequencing is capable of bacterial identification in patients with knee prosthetic joint infection (PJI), and we compared its efficacy with conventional culture studies.</p></div><div><h3>Methods</h3><p>In total, 36 patients who had clinical manifestation suspected of PJI were enrolled in this study. To begin, synovial fluids were aspirated from the affected knee using aseptic technique and tissues specimens were obtained during the surgery. Next, DNA was extracted from the synovial fluid or tissues, and 16S rDNA PCR was performed. In PCR positive cases, nanopore amplicon sequencing was then performed for up to 3 h. The results of amplicon sequencing were compared to those of conventional culture studies.</p></div><div><h3>Results</h3><p>Of the 36 patients enrolled, 22 were classified as true infections according to the MSIS criteria whereas 14 were considered uninfected. Among the 22 PJI cases, 19 cases were culture positive (CP-PJI) while three cases were culture negative (CN-PJI). In 14 of 19 (73.7 %) CP- PJI cases, 16S sequencing identified concordant bacteria with conventional culture studies with a significantly shorter turnaround time. In some cases, nanopore 16S sequencing was superior to culture studies in the species-level identification of pathogen and detection of polymicrobial infections. Altogether, in the majority of PJI candidate patients (32 of 36, 88.9 %), 16S sequencing achieved identical results to cultures studies with a significantly reduced turnaround time (100.9 ± 32.5 h vs. 10.8 ± 7.7 h, p &lt; 0.001).</p></div><div><h3>Conclusions</h3><p>Nanopore 16S sequencing was found to be particularly useful for pathogen identification in knee PJI. Although the sensitivity was not superior to culture studies, the nanopore 16S sequencing was much faster, and species-level identification and detection of polymicrobial infections were superior to culture studies.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"312 8","pages":"Article 151570"},"PeriodicalIF":4.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422122000236/pdfft?md5=cfa0d1d1587c812eb3cc653651f2dc33&pid=1-s2.0-S1438422122000236-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10397728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlamydia psittaci inhibits apoptosis of human neutrophils by activating P2X7 receptor expression","authors":"Zhangping He ,&nbsp;Chuan Wang ,&nbsp;Jianye Wang ,&nbsp;Kang Zheng ,&nbsp;Nan Ding ,&nbsp;Maoying Yu ,&nbsp;Weiwei Li ,&nbsp;Yuanyuan Tang ,&nbsp;Yumeng Li ,&nbsp;Jian Xiao ,&nbsp;Mingxing Liang ,&nbsp;Yimou Wu","doi":"10.1016/j.ijmm.2022.151571","DOIUrl":"10.1016/j.ijmm.2022.151571","url":null,"abstract":"<div><p>This study tested the hypothesis that <em>Chlamydia psittaci</em> (<em>C. psittaci</em>) survives and multiplies in human neutrophils by activating P2X7, a nonselective cationic channel receptor expressed constitutively on the surface of these cells. Findings illustrated that P2X7 receptor expression was enhanced in <em>C. psittaci</em>-infected neutrophils. <em>C. psittaci</em> was able to inhibite spontaneous apoptosis of neutrophils through mitochondrial-induced ATP release and IL-8 production. Importantly, inhibiting ATP activation of the P2X7 receptor with AZ10606120 promotes apoptosis, while stimulating P2X7 receptor expression with BzATP delayed spontaneous apoptosis of human neutrophils, suggesting that <em>C. psittaci</em> inhibits apoptosis of human neutrophils by activating P2X7 receptor. This study reveals new insights into the survival advantages of the latent persistent state of <em>C. psittaci</em> and the mechanism by which it evades the innate immune response<em>.</em></p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"312 8","pages":"Article 151571"},"PeriodicalIF":4.1,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422122000248/pdfft?md5=a7dee146ab183179e7a671e3e1ac4434&pid=1-s2.0-S1438422122000248-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10361454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}