选择性富集培养基中的预孵育是否能提高RIDA®基因PCR对致泻性大肠杆菌的检测?

IF 4.5 3区医学 Q1 MICROBIOLOGY

International Journal of Medical Microbiology Pub Date : 2023-03-01 DOI:10.1016/j.ijmm.2023.151575

Neele J. Froböse , Ioana D. Olaru , Julia Sophie Schneider , Wenlan Zhang , Alexander Mellmann , Franziska Schuler , Tobias Grebe , Frieder Schaumburg

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引用次数: 0

摘要

我们的目的是研究选择性预PCR富集步骤是否能提高RIDA®基因肠出血性大肠杆菌/EPEC从粪便样本中检测腹泻性大肠杆菌的测试性能。在选择性肉汤中进行和不进行预PCR富集的情况下，分析250个粪便样品中的每个样品是否存在stx1/2和eae。与参考方法相比，具有和不具有PCR前富集的stx1/2和eae的敏感性分别为84%（95%CI 70-93）和89%（stx1/2，95%CI 76-96），以及71%（95%CI 58-81）和72%（eae，95%CI 60-82）。方法和靶基因的特异性均超过97%。总之，PCR前肉汤富集并没有提高检测性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Does pre-incubation in selective-enrichment media improve the detection of diarrheagenic Escherichia coli using the RIDA®GENE PCR?

We aimed to investigate whether a selective pre-PCR enrichment step improves test performance of RIDA®GENE EHEC/EPEC to detect diarrheagenic Escherichia coli from stool samples. Each of the 250 stool samples was analyzed for the presence of stx1/2 and eae both with and without pre-PCR enrichment in selective broth. In comparison to a reference method, sensitivities for stx1/2 and eae with and without pre-PCR enrichment were 84% (95%CI 70–93) and 89% (stx1/2, 95%CI 76–96), and 71% (95%CI 58–81) and 72% (eae, 95%CI 60–82), respectively. Specificity exceeded 97% for both methods and target genes. In summary, pre-PCR broth enrichment did not improve test performance.

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来源期刊

International Journal of Medical Microbiology 医学-病毒学

CiteScore

9.70

自引率

0.00%

发文量

审稿时长

45 days

期刊介绍： Pathogen genome sequencing projects have provided a wealth of data that need to be set in context to pathogenicity and the outcome of infections. In addition, the interplay between a pathogen and its host cell has become increasingly important to understand and interfere with diseases caused by microbial pathogens. IJMM meets these needs by focussing on genome and proteome analyses, studies dealing with the molecular mechanisms of pathogenicity and the evolution of pathogenic agents, the interactions between pathogens and host cells ("cellular microbiology"), and molecular epidemiology. To help the reader keeping up with the rapidly evolving new findings in the field of medical microbiology, IJMM publishes original articles, case studies and topical, state-of-the-art mini-reviews in a well balanced fashion. All articles are strictly peer-reviewed. Important topics are reinforced by 2 special issues per year dedicated to a particular theme. Finally, at irregular intervals, current opinions on recent or future developments in medical microbiology are presented in an editorial section.