International Journal of Medical Microbiology最新文献

{"title":"An induced mutation of ABC-transporter component VraF(K84E) contributes to vancomycin resistance and virulence in Staphylococcus aureus strain MW2","authors":"Ruobing Cao ,&nbsp;Huimin Su ,&nbsp;Zichun Wei ,&nbsp;Zhien He ,&nbsp;Ting Pan ,&nbsp;Yujie Li ,&nbsp;Baolin Sun","doi":"10.1016/j.ijmm.2024.151624","DOIUrl":"https://doi.org/10.1016/j.ijmm.2024.151624","url":null,"abstract":"<div><p><em>Staphylococcus aureus</em> is a notorious pathogen responsible for various severe diseases. Due to the emergence of drug-resistant strains, the prevention and treatment of <em>S. aureus</em> infections have become increasingly challenging. Vancomycin is considered to be one of the last-resort drugs for treating most methicillin-resistant <em>S. aureus</em> (MRSA), so it is of great significance to further reveal the mechanism of vancomycin resistance. VraFG is one of the few important ABC (ATP-binding cassette) transporters in <em>S. aureus</em> that can form TCS (two-component systems)/ABC transporter modules. ABC transporters can couple the energy released from ATP hydrolysis to translocate solutes across the cell membrane. In this study, we obtained a strain with decreased vancomycin susceptibility after serial passaging and selection. Subsequently, whole-genome sequencing was performed on this laboratory-derived strain MWA2 and a novel single point mutation was discovered in <em>vraF</em> gene, leading to decreased sensitivity to vancomycin and daptomycin. Furthermore, the mutation reduces autolysis of <em>S. aureus</em> and downregulates the expression of <em>lytM</em>, <em>isaA</em>, and <em>atlA</em>. Additionally, we observed that the mutant has a less net negative surface charge than wild-type strain. We also noted an increase in the expression of the <em>dlt</em> operon and <em>mprF</em> gene, which are associated with cell surface charge and serve to hinder the binding of cationic peptides by promoting electrostatic repulsion. Moreover, this mutation has been shown to enhance hemolytic activity, expand subcutaneous abscesses, reflecting an increased virulence. This study confirms the impact of a point mutation of VraF on <em>S. aureus</em> antibiotic resistance and virulence, contributing to a broader understanding of ABC transporter function and providing new targets for treating <em>S. aureus</em> infections.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"315 ","pages":"Article 151624"},"PeriodicalIF":4.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422124000286/pdfft?md5=e052303279a99f3f97dbf815ad0bceeb&pid=1-s2.0-S1438422124000286-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141249384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of a novel α-haemolytic streptococci, Streptococcus parapneumoniae sp. nov., which caused bacteremia with pyelonephritis","authors":"Yuri Katayama ,&nbsp;Masatomo Morita ,&nbsp;Bin Chang ,&nbsp;Daisuke Katagiri ,&nbsp;Masahiro Ishikane ,&nbsp;Gen Yamada ,&nbsp;Kazuhisa Mezaki ,&nbsp;Masami Kurokawa ,&nbsp;Hideki Takano ,&nbsp;Yukihiro Akeda","doi":"10.1016/j.ijmm.2024.151625","DOIUrl":"10.1016/j.ijmm.2024.151625","url":null,"abstract":"<div><h3>Objectives</h3><p>We report a case of bacteremia with pyelonephritis in an adult male with an underlying disease caused by α-hemolytic streptococci. α-Hemolytic streptococci were isolated from blood, but it was challenging to identify its species. This study aimed to characterize the causative bacterium SP4011 and to elucidate its species.</p></div><div><h3>Methods</h3><p>The whole-genome sequence and biochemical characteristics of SP4011 were determined. Based on the genome sequence, phylogenetic analysis was performed with standard strains of each species of α-hemolytic streptococci. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values were calculated.</p></div><div><h3>Results</h3><p>SP4011 showed optochin susceptibility and bile solubility, but did not react with pneumococcal omni antiserum. Phylogenetic analysis of the whole-genome sequence showed that SP4011 clustered with <em>S. pneumoniae</em> and <em>S. pseodopneumoniae</em> and was most closely related to <em>S. pseodopneumoniae</em>. Genomic analysis revealed that ANI and dDDH values between SP4011 and <em>S. pseodopneumoniae</em> were 94.0 % and 56.0 %, respectively, and between SP4011 and <em>S. pneumoniae</em> were 93.3 % and 52.2 %, respectively. Biochemical characteristics also showed differences between SP4011 and <em>S. pseodopneumoniae</em> and between SP4011 and <em>S. pneumoniae</em>. These results indicate that SP4011 is a novel species.</p></div><div><h3>Conclusion</h3><p>Our findings indicate that SP4011 is a novel species of the genus <em>Streptococcus</em>. SP4011 has biochemical characteristics similar to <em>S. pneumoniae</em>, making it challenging to differentiate and requiring careful clinical diagnosis. This isolate was proposed to be a novel species, <em>Streptococcus parapneumoniae</em> sp. nov. The strain type is SP4011^T (= JCM 36068^T = KCTC 21228^T).</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"315 ","pages":"Article 151625"},"PeriodicalIF":4.1,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422124000298/pdfft?md5=d0d1beaf745a144a1f225eb61d226e0e&pid=1-s2.0-S1438422124000298-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141194645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance and virulence genes in Helicobacter pylori strains isolated from children in Shanghai, China (2019–2022)","authors":"Chunling Li ,&nbsp;Leiyan He ,&nbsp;Aimin Wang ,&nbsp;Saige Chen ,&nbsp;Pan Fu ,&nbsp;Chuanqing Wang","doi":"10.1016/j.ijmm.2024.151622","DOIUrl":"https://doi.org/10.1016/j.ijmm.2024.151622","url":null,"abstract":"<div><h3>Background</h3><p>The increasing prevalence of antibiotic-resistant <em>Helicobacter pylori</em> strains poses a significant threat to children's health. This study investigated antibiotic resistance rates in <em>Helicobacter pylori</em> strains isolated from children in Shanghai and analyzed the presence of virulence genes in these strains.</p></div><div><h3>Methods</h3><p>We obtained 201 <em>Helicobacter pylori</em> strains from pediatric patients with upper gastrointestinal symptoms who underwent gastrointestinal endoscopy between 2019 and 2022. Subsequently, we performed antibiotic susceptibility tests and virulence gene PCR assays on these strains.</p></div><div><h3>Results</h3><p><em>Helicobacter pylori</em> resistance rates of 45.8%, 15.4%, 1.0%, and 2.5% were detected for metronidazole, clarithromycin, amoxicillin, and levofloxacin, respectively. Among all isolates, 64.7% exhibited resistance to at least one antibiotic. Resistance to metronidazole and clarithromycin increased from 2019 to 2022. The predominant vacA gene subtype was vacA s1a/m2. The prevalence of vacA m2 and dupA exhibited an upward trend, while oipA presented a decreasing trend from 2019 to 2022. The prevalence of dupA was significantly higher in gastritis than peptic ulcer disease, and in non-treatment compared to treatment groups.</p></div><div><h3>Conclusions</h3><p><em>Helicobacter pylori</em> antibiotic resistance remains high in children and has risen in recent years. Therefore, the increasing use of metronidazole and clarithromycin requires increased monitoring in children. No association was observed between antibiotic resistance and virulence gene phenotypes.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"315 ","pages":"Article 151622"},"PeriodicalIF":4.1,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422124000262/pdfft?md5=46e615129698b18d32d76c3c7d1a127c&pid=1-s2.0-S1438422124000262-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141078465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and characterization of community-associated Staphylococcus aureus isolates from human mastitis in Beijing, China","authors":"Jihong Gu ,&nbsp;Mengyuan Xiong ,&nbsp;Jing Zhang ,&nbsp;Yirong Li","doi":"10.1016/j.ijmm.2024.151623","DOIUrl":"https://doi.org/10.1016/j.ijmm.2024.151623","url":null,"abstract":"<div><h3>Objectives</h3><p><em>Staphylococcus aureus (S. aureus)</em> spreads worldwide and occurrence of mastitis caused by it holds significant implications for public health. We aim to reveal the molecular typing, antibiotic resistance and virulence gene profile of <em>S. aureus</em> causing mastitis through investigation.</p></div><div><h3>Methods</h3><p>A total of 200 isolates of <em>S. aureus</em> were collected from outpatients infected with mastitis in a hospital in Beijing from 2020.7 to 2021.7. The molecular characteristics were analyzed by MLST and spa typing, virulence genes were screened by PCR, antibiotic susceptible test was performed by VITEK® 2 Compact system and phylogenetic analysis was performed by MEGA11 and iTOL.</p></div><div><h3>Results</h3><p>Nineteen sequence types (STs) belonging to 9 clone complexes (CCs) were identified. ST22 was the most dominant clone (77.0%, 154/200). MRSA accounted for 19.0% (38/200) and 89.5% (34/38) of MRSA isolates belonged to CC22 and CC59. The isolates had relatively low levels of antibiotic resistance, with the exception of β-lactams and macrolides with resistance rates above 50.0%. The carrying rate of <em>pvl</em> in the ST22-MRSA strains were 84.2% and the detection rates of <em>seb</em> and <em>pvl</em> in the MRSA isolates were significantly higher than those in the MSSA isolates, while the <em>hlg, fnbA</em> and <em>sdrD</em> showed opposite results. Whole genome sequenced specimens of MRSA strains X4 and B5 show the same evolutionary origin as ST22 EMRSA-15 (HE681097), which is popular in Europe.</p></div><div><h3>Conclusions</h3><p>The method based on molecular epidemiology is an important tool for tracking the spread of <em>S. aureus</em> infections. We need to be alert to the major MRSA clones CC22 and CC59 in the region and be vigilant to the possible pandemic and spread of ST22 EMRSA-15.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"315 ","pages":"Article 151623"},"PeriodicalIF":4.1,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422124000274/pdfft?md5=49d7110665d517da6a0373359b3db678&pid=1-s2.0-S1438422124000274-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141078466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic impact of delivery modes on gut microbiota in preterm infants hospitalized during the initial 4 weeks of life","authors":"Xin Wu ,&nbsp;Rui Guo ,&nbsp;Yijia Fan ,&nbsp;Shuang Chen ,&nbsp;Wei Zheng ,&nbsp;Xiaoli Shu ,&nbsp;Bo Chen ,&nbsp;Xing Li ,&nbsp;Tingting Xu ,&nbsp;Lingbing Shi ,&nbsp;Li Chen ,&nbsp;Lichun Shan ,&nbsp;Zhenya Zhu ,&nbsp;Enfu Tao ,&nbsp;Mizu Jiang","doi":"10.1016/j.ijmm.2024.151621","DOIUrl":"https://doi.org/10.1016/j.ijmm.2024.151621","url":null,"abstract":"<div><p>Preterm infants face a high risk of various complications, and their gut microbiota plays a pivotal role in health. Delivery modes have been reported to affect the development of gut microbiota in term infants, but its impact on preterm infants remains unclear. Here, we collected fecal samples from 30 preterm infants at five-time points within the first four weeks of life. Employing 16 S rRNA sequencing, principal coordinates analysis, the analysis of similarities, and the Wilcoxon rank-sum test, we examined the top dominant phyla and genera, the temporal changes in specific taxa abundance, and their relationship with delivery modes, such as <em>Escherichia-Shigella</em> and <em>Enterococcus</em> based on vaginal delivery and <em>Pluralibacter</em> related to cesarean section. Moreover, we identified particular bacteria, such as <em>Taonella</em>, <em>Patulibacter</em>, and others, whose proportions fluctuated among preterm infants born via different delivery modes at varying time points, as well as the microbiota types and functions. These results indicated the influence of delivery mode on the composition and function of the preterm infant gut microbiota. Importantly, these effects are time-dependent during the early stages of life. These insights shed light on the pivotal role of delivery mode in shaping the gut microbiota of preterm infants and have significant clinical implications for their care and management.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"315 ","pages":"Article 151621"},"PeriodicalIF":4.1,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422124000250/pdfft?md5=1faac72c5542d3284f0fe49374ccb163&pid=1-s2.0-S1438422124000250-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140950208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative transcriptomic analysis of Staphylococcus epidermidis associated with periprosthetic joint infection under in vivo and in vitro conditions","authors":"Cody R. Fisher ,&nbsp;Thao L. Masters ,&nbsp;Stephen Johnson ,&nbsp;Kerryl E. Greenwood-Quaintance ,&nbsp;Nicholas Chia ,&nbsp;Matthew P. Abdel ,&nbsp;Robin Patel","doi":"10.1016/j.ijmm.2024.151620","DOIUrl":"https://doi.org/10.1016/j.ijmm.2024.151620","url":null,"abstract":"<div><p><em>Staphylococcus epidermidis</em> is part of the commensal microbiota of the skin and mucous membranes, though it can also act as a pathogen in certain scenarios, causing a range of infections, including periprosthetic joint infection (PJI). Transcriptomic profiling may provide insights into mechanisms by which <em>S. epidermidis</em> adapts while in a pathogenic compared to a commensal state. Here, a total RNA-sequencing approach was used to profile and compare the transcriptomes of 19 paired PJI-associated <em>S. epidermidis</em> samples from an <em>in vivo</em> clinical source and grown in <em>in vitro</em> laboratory culture. Genomic comparison of PJI-associated and publicly available commensal-state isolates were also compared. Of the 1919 total transcripts found, 145 were from differentially expressed genes (DEGs) when comparing <em>in vivo</em> or <em>in vitro</em> samples. Forty-two transcripts were upregulated and 103 downregulated in <em>in vivo</em> samples. Of note, metal sequestration-associated genes, specifically those related to staphylopine activity (<em>cntA</em>, cntK, <em>cntL</em>, and <em>cntM</em>), were upregulated in a subset of clinical <em>in vivo</em> compared to laboratory grown <em>in vitro</em> samples. About 70% of the total transcripts and almost 50% of the DEGs identified have not yet been annotated. There were no significant genomic differences between known commensal and PJI-associated <em>S. epidermidis</em> isolates, suggesting that differential genomics may not play a role in <em>S. epidermidis</em> pathogenicity. In conclusion, this study provides insights into phenotypic alterations employed by <em>S epidermidis</em> to adapt to infective and non-infected microenvironments, potentially informing future therapeutic targets for related infections.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"315 ","pages":"Article 151620"},"PeriodicalIF":4.1,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422124000249/pdfft?md5=126cebbcf4313f3fec2494abad1a87d7&pid=1-s2.0-S1438422124000249-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140345244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of BCP/PreC/C region quasispecies in treatment-naive patients with different phases of HBV infection using next-generation sequencing","authors":"Chenggong Zhu ,&nbsp;Minjie Tang ,&nbsp;Ya Fu ,&nbsp;Zhen Xun ,&nbsp;Caorui Lin ,&nbsp;Songhang Wu ,&nbsp;Tianbin Chen ,&nbsp;Yongbin Zeng ,&nbsp;Bin Yang ,&nbsp;Qishui Ou ,&nbsp;Can Liu","doi":"10.1016/j.ijmm.2024.151619","DOIUrl":"https://doi.org/10.1016/j.ijmm.2024.151619","url":null,"abstract":"<div><h3>Background</h3><p>To analysis of quasispecies (QS) changes and high-frequency mutations in the BCP/PreC/C region of patients at different phases of hepatitis B virus (HBV) infection and provides novel biomarkers for the diagnosis of chronic hepatitis B (CHB) patients.</p></div><div><h3>Methods</h3><p>With the application of next-generation sequencing technology, we were able to sequence the HBV BCP/PreC/C regions in 40 patients, each at different phases of the HBV infection. The heterogeneity of QS and the frequency of mutations were calculated using MEGA 7 software.</p></div><div><h3>Results</h3><p>Our results show that the complexity and diversity of the BCP/PreC/C QS in HBeAg-positive CHB patients are significantly higher than those in HBeAg-positive chronic infection patients, while HBeAg-negative chronic infection patients had significantly higher QS complexity and diversity than HBeAg-negative CHB patients. In addition, HBeAg-negative patients showed reduced complexity but increased diversity compared with HBeAg-positive patients. Receiver operating characteristic curves showed that G1764A, C2102T, <em>dN</em> and complexity of QS could be used as potential biomarkers for diagnosing HBeAg-positive CHB, while the A2189C, <em>dS</em> and complexity of QS could be used as potential biomarkers for diagnosing HBeAg-negative chronic hepatitis. Finally, our study also found that G1896A and A2159G may be hotspot mutations affecting HBeAg seroconversion.</p></div><div><h3>Conclusion</h3><p>Our research elucidates the evolution of HBV by analyzing QS heterogeneity and mutation patterns, offering novel serum biomarkers for enhancing clinical diagnosis and disease prognosis. This comprehensive approach sheds light on the intricate dynamics of HBV progression and paves the way for more precise medical interventions.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"315 ","pages":"Article 151619"},"PeriodicalIF":4.1,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422124000237/pdfft?md5=ff10bfb68cddde73a04c5dd307f1a826&pid=1-s2.0-S1438422124000237-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140338900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and immunogenicity evaluation of C-HapS-P6 fusion protein vaccine against nontypeable Haemophilus influenzae in mice","authors":"Nan Hu,&nbsp;Weifeng Li,&nbsp;Zihong Zhao,&nbsp;Yueli Chang,&nbsp;Cai Wang,&nbsp;Yutuo Zhang","doi":"10.1016/j.ijmm.2024.151616","DOIUrl":"https://doi.org/10.1016/j.ijmm.2024.151616","url":null,"abstract":"<div><p>Nontypeable <em>Haemophilus influenzae</em> (NTHi) is the dominant pathogen in several infectious diseases. Currently the use of antibiotics is the main intervention to prevent NTHi infections, however with the emergence of drug resistant strains, it has compromised the treatment of respiratory infections with antibiotics. Therefore there is an urgent need to develop a safe and effective vaccine to prevent NTHi infections. We investigate the potential of C-HapS-P6 fusion protein as a vaccine for treating NTHi in murine models. PGEX-6P2/C-HapS-P6 fusion gene was constructed using overlap extension polymerase chain reaction. The recombined plasmid was transformed into <em>Escherichia coli</em> for protein expression. The mice were subjected to intraperitoneal immunization using purified antigens. Immunoglobulin (Ig) G in serum samples and IgA in nasal and lung lavage fluids were analyzed using enzyme-linked immunosorbent assay. Cytokine release and proliferation capacity of splenic lymphocytes in response to antigens were measured <em>in vitro</em>. The protective effect of the C-HapS-P6 protein against NTHi infection was evaluated by NTHi count and histological examination. The data showed that the C-HapS-P6 fusion protein increased significantly the levels of serum IgG and nasal and lung IgA, and promoted the release of interleukin (IL)-2, interferon-ϒ, IL-4, IL-5, and IL-17 and the proliferation of splenic lymphocytes compared with C-HapS or P6 protein treatment alone. Moreover, C-HapS-P6 effectively reduced the NTHi colonization in the nasopharynx and lungs of mice. In conclusion, our results demonstrated that the C-HapS-P6 fusion protein vaccine can significantly enhance humoral and cell immune responses and effectively prevent against NTHi infection in the respiratory tract in murine models.</p></div>","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"314 ","pages":"Article 151616"},"PeriodicalIF":4.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422124000201/pdfft?md5=d730d8c08565c267e2180866754e0738&pid=1-s2.0-S1438422124000201-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140066968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Public Health Microbiology: Recent developments under Robert Koch’s genius loci","authors":"Annette Mankertz,&nbsp;Lars Schaade","doi":"10.1016/j.ijmm.2024.151613","DOIUrl":"10.1016/j.ijmm.2024.151613","url":null,"abstract":"","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"314 ","pages":"Article 151613"},"PeriodicalIF":4.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422124000171/pdfft?md5=39c4e92c950b86d812b938335dd1d099&pid=1-s2.0-S1438422124000171-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139956108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AL DISCRETO LETTORE: A short-list on Public Health Microbiology in Germany","authors":"Anton Aebischer ,&nbsp;Annette Mankertz ,&nbsp;Guido Werner ,&nbsp;Sebastian Suerbaum","doi":"10.1016/j.ijmm.2024.151617","DOIUrl":"10.1016/j.ijmm.2024.151617","url":null,"abstract":"","PeriodicalId":50312,"journal":{"name":"International Journal of Medical Microbiology","volume":"314 ","pages":"Article 151617"},"PeriodicalIF":4.1,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1438422124000213/pdfft?md5=278e7e8d4867e2bbad755c1428be378a&pid=1-s2.0-S1438422124000213-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140074183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}