Reyhaneh Dehghan, Khosro Piri, Asghar Abdoli, Saman Hosseinkhani
{"title":"Parameters Optimization for Improving Bioluminescence Inhibition Assay Using Vibrio fischeri Bacteria to Detect Lipopolysaccharide Toxicity in Aquatic Environments.","authors":"Reyhaneh Dehghan, Khosro Piri, Asghar Abdoli, Saman Hosseinkhani","doi":"10.1007/s00128-024-03970-2","DOIUrl":"10.1007/s00128-024-03970-2","url":null,"abstract":"<p><p>Bioluminescence inhibition of Vibrio fischeri is a widely used method for toxicity testing in aquatic environments. Certain complex biological contaminants, such as lipopolysaccharide (LPS), can interfere with metabolic pathways during toxicity assays. The standard 15-minute Vibrio fischeri bioluminescence assay has limitations when evaluating and screening water toxicity against complex and emerging chemicals like LPS. To accurately determine the effects of such substances, it is crucial to use a bioassay that encompasses a sufficient cell cycle period. This study tested LPS at varying incubation times (ranging from 60 s to 60 min) and concentrations (1-1*10<sup>- 12</sup> mg/ml) to identify the appropriate incubation time for bioluminescence inhibition and toxicity testing. The results indicated that bioluminescence inhibition begins within 60 s and reaches maximum inhibition at 60 min. However, at 30 and 45 min, the bacterial response to different concentrations of LPS varied, with some concentrations causing increased bioluminescence. The EC<sub>50</sub> values at different times (60 s, 15, 30, 45, and 60 min) were found to be 0.0012, 0.0063, 4.07e + 54, 3.85e-8, and 3.34e-9 mg/ml respectively. This study highlights the importance of considering incubation time when using bioluminescence inhibition to detect acute toxicity in aquatic ecosystems. A longer incubation time may enhance the method's sensitivity and improve its ability to detect low levels of toxins, such as LPS, in water resources.</p>","PeriodicalId":501,"journal":{"name":"Bulletin of Environmental Contamination and Toxicology","volume":"113 5","pages":"61"},"PeriodicalIF":2.7,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Priya Varghese, Kundan Kumar, Pritam Sarkar, Sutanu Karmakar, S P Shukla, Saurav Kumar, Vidya Shree Bharti, Tapas Paul, Debiprasad Kantal
{"title":"Impact of Triclosan on Bacterial Biodiversity and Sediment Enzymes - A Microcosm Study.","authors":"Priya Varghese, Kundan Kumar, Pritam Sarkar, Sutanu Karmakar, S P Shukla, Saurav Kumar, Vidya Shree Bharti, Tapas Paul, Debiprasad Kantal","doi":"10.1007/s00128-024-03969-9","DOIUrl":"10.1007/s00128-024-03969-9","url":null,"abstract":"<p><p>Triclosan (TCS), a widely used antimicrobial biocide, has raised serious concern among the scientific community in recent years owing to its ubiquitous presence around the globe and toxicity to aquatic organisms. The current study investigated the alterations in bacterial diversity, nutrients, and sediment enzyme activity in TCS-exposed sediment. TCS concentrations of 3 mg/L (T1) and 6 mg/L (T2) were applied in a microcosm setup for 28 days to sediment collected from Versova Creek, Mumbai. Among sediment enzymes, dehydrogenase activity exhibited the greatest degree of variability in 3 mg/L exposed sediment. Nitrite, total nitrogen and urease exhibited higher concentrations in 6 mg/L TCS exposed sediment. The concentration of ammonia was observed to be decreasing in treatments exposed to 6 mg/L TCS. Total heterotrophic bacteria exhibited an increase in count in T1 and a decrease in T2. Metagenomics data showed a higher relative abundance of bacteria in T1 compared to T2 on the 28th day of sampling. Proteobacteria was found to be the most abundant phylum in all samples, and their relative abundance was reduced by 0.14% in T<sub>1</sub> and 5.48% in T<sub>2</sub>. The results confirm the alterations in the composition of sediment bacterial communities and their enzymatic activities due to TCS exposure.</p>","PeriodicalId":501,"journal":{"name":"Bulletin of Environmental Contamination and Toxicology","volume":"113 5","pages":"59"},"PeriodicalIF":2.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junwei Tang, Chaolan Zhang, Xutong Liu, Xianghua Wu, Yibo Zhang, Tao Zhang, Ruijing Xia, Kai Zhong
{"title":"The fate of Cd in Soils with Various Particle Sizes: Characteristics, Speciation Distribution and Influencing Factors.","authors":"Junwei Tang, Chaolan Zhang, Xutong Liu, Xianghua Wu, Yibo Zhang, Tao Zhang, Ruijing Xia, Kai Zhong","doi":"10.1007/s00128-024-03968-w","DOIUrl":"10.1007/s00128-024-03968-w","url":null,"abstract":"<p><p>This study investigated the distribution of Cd in soil water-stable aggregate particles of varying sizes, revealing that smaller particles have higher total Cd content as well as different forms of Cd content, with the clay particle showing a greater tendency to accumulate Cd. However, the proportion of high activity Cd is lower in clay particles, posing a lower environmental risk of Cd transformation compared to silt particles. Adsorption experiments indicated that the clay particle exhibits the strongest adsorption capacity and highest adsorption rate. Additionally, correlation and principal component analyses identified Fe-Mn oxides and organic matter as the primary influencing factors on Cd distribution characteristics, with pH playing a secondary role. These findings provide valuable insights for the remediation of heavy metal-contaminated soil.</p>","PeriodicalId":501,"journal":{"name":"Bulletin of Environmental Contamination and Toxicology","volume":"113 5","pages":"60"},"PeriodicalIF":2.7,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Illegal Substance Analysis and Environmental Risk Assessment in Küçük Menderes River, Important Basin of the Aegean Region.","authors":"Duygu Yeşim Ovat, Rukiye Aslan, Melike Aydoğdu, Serap Annette Akgür","doi":"10.1007/s00128-024-03961-3","DOIUrl":"10.1007/s00128-024-03961-3","url":null,"abstract":"<p><p>Water-based studies have come into prominence for illegal substance monitoring. There are limited studies on the detection of these substances in the surface waters as opposed to wastewater. This study aims to evaluate amphetamine, benzoylecgonine, cocaine, codeine, 3,4-methylenedioxy-N-methylamphetamine, morphine, and 11-nor-Δ9- tetrahydrocannabinol-9-carboxylic acid in the Küçük Menderes (KM) River at four different stations during three different periods and assess the environmental risks. Environmental risk assessment of psychoactive substances based on the calculation of Risk Quotients (RQ) were evaluated. RQ values for amphetamine and benzoylecgonine were < 0.01 at all sampling points during all sampling periods, indicating little risk for aquatic life. Cocaine was found as environmentally high risk (RQ > 1) based on its RQ values of 1.22 and 1.37 at KM-01 and KM-02 sampling points. This research is the first report to investigate the presence of psychoactive substances and define the environmental risks of these substances in Türkiye.</p>","PeriodicalId":501,"journal":{"name":"Bulletin of Environmental Contamination and Toxicology","volume":"113 5","pages":"56"},"PeriodicalIF":2.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M Sacristan de Alva, I Oceguera-Vargas, E Lamas-Cosío, K León-Aguirre, F Arcega-Cabrera
{"title":"Removal of Nitrogen, Phosphorus, Organic Matter, and Heavy Metals from Pig-Farming Wastewater Using a Microalgae-Bacteria Consortium.","authors":"M Sacristan de Alva, I Oceguera-Vargas, E Lamas-Cosío, K León-Aguirre, F Arcega-Cabrera","doi":"10.1007/s00128-024-03962-2","DOIUrl":"10.1007/s00128-024-03962-2","url":null,"abstract":"<p><p>Wastewater generated by the pork industry urgently requires the implementation of low-cost, high-benefit, and efficient treatment systems. Accordingly, a microalgae-bacteria consortia-based treatment system is proposed for the removal of contaminants released, by the pork-producing industry, in swine wastewater. In this study, different inoculum concentrations of the microalgae-bacteria consortium were tested to document variation in the removal of nutrients from the wastewater. At varying concentrations, it was efficient and did not present a significant difference in the removal kinetics. The treatment with the greatest amount of inoculum removed close to 87% of total nitrogen, approximately 70% of orthophosphate, and 77% of chemical oxygen demand. Removals of 84% iron, 44% copper, and 48% manganese were also obtained. These results demonstrate that microalgae-bacteria consortia are an economically viable and environmentally desirable option for the efficient treatment of wastewater from the pork industry.</p>","PeriodicalId":501,"journal":{"name":"Bulletin of Environmental Contamination and Toxicology","volume":"113 5","pages":"58"},"PeriodicalIF":2.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11490518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mayra Alejandra Cañizares-Martínez, Mercedes Amparo Quintanilla-Mena, Mónica Améndola-Pimenta, Rossanna Rodríguez-Canul, Flor Árcega-Cabrera, Marcela Del Río-García, Victor Ceja-Moreno, M Leopoldina Aguirre-Macedo, Carlos Alberto Puch-Hau
{"title":"Multiple-Integrated Biomarker Indexes to Assess the Responses of the Flatfish Achirus lineatus during Exposure to Light Crude Oil Water Accommodated Fraction.","authors":"Mayra Alejandra Cañizares-Martínez, Mercedes Amparo Quintanilla-Mena, Mónica Améndola-Pimenta, Rossanna Rodríguez-Canul, Flor Árcega-Cabrera, Marcela Del Río-García, Victor Ceja-Moreno, M Leopoldina Aguirre-Macedo, Carlos Alberto Puch-Hau","doi":"10.1007/s00128-024-03967-x","DOIUrl":"10.1007/s00128-024-03967-x","url":null,"abstract":"<p><p>In the present study, we evaluated the biological response of Achirus lineatus to water accommodated fraction (WAF) of light crude oil (American Petroleum Institute gravity 35°) during a sub-chronic bioassay (14 and 28 days) at two different concentrations: 5% v/v (1.20 µg∙L<sup>- 1</sup> expressed as total polycyclic aromatic hydrocarbons [∑25 PAH]) and 10% v/v (6.61 µg∙L<sup>- 1</sup> [∑25 PAH]). The responses were evaluated through the biomarker response index (BRI), the integrated biomarker response (IBRv2) and the bioconcentration factor (BCF). The results showed an increase in biological response in relation to WAF concentration and exposure time, which resulted in a slight and moderate disturbance in the basal condition and bioconcentration level of metals (Pb > Ni > V > Cd) in fish tissue. Results in the present study denote that flatfish such as A. lineatus may be negatively influenced by spilled light crude oil.</p>","PeriodicalId":501,"journal":{"name":"Bulletin of Environmental Contamination and Toxicology","volume":"113 5","pages":"57"},"PeriodicalIF":2.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mobility, Bioavailability, and Enrichment of Soil-Bound Phosphorus in Flood-Prone Paddy Fields: A Case Study of Kunnukara, South India.","authors":"Anjali Thaitharanikathil Babu, Anand Madhavan, Nihala Bai, Madhuraj Palat Kannankai, Akhilesh Kottikkathara Bhanuvikraman, Ratheesh Kumar Chelakkal Sukumaran","doi":"10.1007/s00128-024-03965-z","DOIUrl":"10.1007/s00128-024-03965-z","url":null,"abstract":"<p><p>Bioavailability, enrichment, and risk of phosphorus (P) and its fraction composition were monitored in the paddy soils of Kunnukara during the pre-cultivation and post-harvest periods in the years 2020 and 2021. Iron-bound P (≥ 105.56 ± 0.05 mg/kg) was found highest among the P fractions. The bioavailability of P was recorded at peak value during the post-harvest period, contributed by organic P, Iron bound P, and loosely bound P. Principal component analysis inferred that loosely bound P was pH-dependent and significantly influenced by cation exchange, particle density, soil aggregate stability, and total organic carbon (TOC) in the post-harvest soil, whereas TOC, aluminium-bound P, and calcium-bound P in the pre-cultivation soils. Additionally, physico-chemical parameters like electrical conductivity, bulk density, specific gravity, TOC, and soil aggregate stability have a significant impact on the composition of P fractions in the soil. Bioavailable phosphorus (BAP) ranged from 642.78 ± 0.49 to 594.20 ± 1.23 mg/kg during the post-harvest period. Moreover, the contribution of BAP to total P ranged from 99.45 to 99.54%, indicating the fact that soil is sufficient in BAP. Pollution indices revealed that the paddy soils are at risk of eutrophication. Phosphorus Pollution Index (PPI) > 1 exhibited moderate pollution (1.06 to 1.07) at the topsoil (0-15 cm) and PPI < 1, mild pollution (0.92 to 0.99) at 15-30 cm depths. The organic nitrogen index ≥ 0.133 indicates severe soil pollution in the study site. An extended fertilizer application in the field contributes to nutrient enrichment and warrants the risk of contamination in nearby riverine systems (River Periyar and River Chalakkudy).</p>","PeriodicalId":501,"journal":{"name":"Bulletin of Environmental Contamination and Toxicology","volume":"113 5","pages":"55"},"PeriodicalIF":2.7,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shima Akbarimorad, Soheil Sobhanardakani, David Bolonio Martín, Nayereh Sadat Hosseini
{"title":"Contamination Features and Quantitative Source Apportionment of Potentially Toxic Elements in the Urban Surface Soil, the Case of Hamedan, West of Iran.","authors":"Shima Akbarimorad, Soheil Sobhanardakani, David Bolonio Martín, Nayereh Sadat Hosseini","doi":"10.1007/s00128-024-03964-0","DOIUrl":"10.1007/s00128-024-03964-0","url":null,"abstract":"<p><p>In this study, a total of 180 surface soil samples were collected from a control area and residential, commercial, and industrial regions of Hamedan, Iran during the fall season in 2023. Contents of analyzed elements were then determined using ICP-OES. The results illustrated that the average contents of As, Cd, Cu, Mn, Ni, and Pb were 1.17-2.26 times greater than those reported as local background values, while the mean contents of As, Cd, and Cu were respectively 3.41, 1.25, and 1.00 times greater than the background contents for Iran by implying the possible human sources of these PTEs. The cumulative average ecological risk value with 81.9, demonstrated moderate ecological risk across the study area. The results of source apportionment showed that the PTEs contamination in the soil of the study area mainly originates from the anthropogenic activities (65.6%) and traffic emissions as the primary pollution source (47.3%) had the highest contribution to the PTE pollution in the study area. In conclusion, by providing a useful approach to identifying the sources and contributions of toxic elements across different functional areas, this study has the potential to guide future efforts aimed at managing and mitigating the pollution caused by metal elements.</p>","PeriodicalId":501,"journal":{"name":"Bulletin of Environmental Contamination and Toxicology","volume":"113 5","pages":"54"},"PeriodicalIF":2.7,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of Polyethylene Microplastics on Growth and Antioxydant Response of Oscillatoria Princeps and Chlorella Pyrenoidosa.","authors":"Mengxin Zhao, Zimu Ren, Zhangdong Wei, Haolin Shi, Lin Wang, Yixin Liang","doi":"10.1007/s00128-024-03959-x","DOIUrl":"10.1007/s00128-024-03959-x","url":null,"abstract":"<p><p>This study investigated the impacts of polyethylene microplastics (PE-MPs) with varying particle sizes (13 μm and 6.5 μm) on the growth and antioxidant responses of two freshwater algae species, Oscillatoria princeps (O. princeps) and Chlorella pyrenoidosa (C. pyrenoidosa). The results revealed a significant reduction in chlorophyll a content in both algal species upon exposure to PE-MPs, indicating a disruption of photosynthesis. Furthermore, Superoxide Dismutase (SOD) activity decreased in O. princeps, while Catalase (CAT) activity increased in both species, indicating complex physiological responses to microplastic stress. Notably, phycotoxin levels in O. princeps decreased with PE-MP exposure, while those in C. pyrenoidosa increased, particularly with 6.5 μm PE-MPs. These findings underscore the potential toxic effects of PE-MPs on freshwater algal growth and metabolism, as well as their influence on toxin production. This study contributes valuable insights into the ecotoxicological impacts of microplastics in freshwater environments, highlighting the need for further research on their biological effects and environmental health implications.</p>","PeriodicalId":501,"journal":{"name":"Bulletin of Environmental Contamination and Toxicology","volume":"113 5","pages":"53"},"PeriodicalIF":2.7,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hecheng Fu, Jiale Fan, Jie Li, Jianhong Huang, Senlin Tian, Ping Ning
{"title":"Applewood Biochar at Different Smoldering Conditions Passivates Pyrite by Promoting the Formation of Jarosite.","authors":"Hecheng Fu, Jiale Fan, Jie Li, Jianhong Huang, Senlin Tian, Ping Ning","doi":"10.1007/s00128-024-03956-0","DOIUrl":"10.1007/s00128-024-03956-0","url":null,"abstract":"<p><p>To control acid mine drainage (AMD) from the source, a new environmentally and green passivator (biochar) has been introduced to passivate pyrite. To reduce the difficulty of biochar preparation and cost, and improve its production scale, in-situ pyrolysis of applewood by smoldering to produce biochar. Here, particle size, moisture content and gas flow rate were selected to prepare biochar by smoldering through orthogonal combination, and the pyrite was passivated with different conditions and biochar concentrations (2 g/L, 3 g/L, 4 g/L). The results revealed that when the particle size is 200 mm×200 mm×20 mm, the water content is 20-30%, and the gas flow rate is 0.4 L/m<sup>3</sup>, the biochar yield is the highest. Biochar promotes the formation of passivating layer (jarosite), inhibits the release of metal ions. Increasing biochar concentration can promote the formation of jarosite and enhance the passivation effect on pyrite.</p>","PeriodicalId":501,"journal":{"name":"Bulletin of Environmental Contamination and Toxicology","volume":"113 4","pages":"50"},"PeriodicalIF":2.7,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142405875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}