Journal of Marine Research最新文献

{"title":"Senyawa Bioaktif Kappaphycus alvarezii (Doty) Doty, 1988 Sebagai Antibakteri","authors":"Annisa Marhaeny, Rini Pramesti, S. Suryono","doi":"10.14710/jmr.v13i1.40459","DOIUrl":"https://doi.org/10.14710/jmr.v13i1.40459","url":null,"abstract":"Bakteri yang menyebabkan penyakit pada inangnya disebut patogen. Penelitian ini bertujuan mengetahui senyawa bioaktif dan aktivitas antibakteri yang terkandung dalam ekstrak metanol K. alvarezii. Penelitian dilakukan dengan metode deskriptif eksploratif. Bakteri yang diujikan yaitu Staphylococcus aureus dan Escherichia coli. Hasil ekstraksi dilanjutkan dengan uji fitokimia. Ekstrak diencerkan menggunakan dimetil sulfoksida (DMSO) sampai diperoleh konsentrasi uji 500 µg/disk, 1000 µg/disk, dan 1500 µg/disk. Cefadroxil dan DMSO digunakan sebagai kontrol positif dan kontrol negatif. Metode difusi cakram digunakan untuk uji aktivitas antibakteri. Inokulum diinkubasi selama 3x24 jam pada suhu 37ºC. Zona hambat dihitung setiap 24 jam sekali. Hasil uji aktivitas antibakteri ekstrak metanol K. alvarezii terhadap bakteri S. aureus dan E. coli menunjukkan zona hambat terbesar pada konsentrasi 500 µg/disk pada waktu 48 jam sebesar 4,95 mm dan 5,1 mm. Senyawa bioaktif yang terkandung dalam ekstrak metanol K. alvarezii yaitu senyawa alkaloid, flavonoid, steroid dan tanin. Bacteria that cause disease in their hosts are called pathogens. This study aims to determine the bioactive compounds and antibacterial activity in the methanol extract of K. alvarezii. The research was conducted using a exploratory descriptive method. The bacteria tested were Staphylococcus aureus and Escherichia coli. Extraction then phytochemical tests were carried out The extract was diluted using dimethyl sulfoxide (DMSO) to obtain test concentrations of 500 µg/disk, 1000 µg/disk, and 1500 µg/disk. Cefadroxil and DMSO were used as positive and negative controls. Antibacterial activity test using disc diffusion method. The inoculum was incubated for 3x24 hours at 37°C. The inhibition zone is calculated every 24 hours. The results of the antibacterial activity test of K. alvarezii methanol extract against S. aureus and E. coli bacteria showed the largest inhibition zone at a concentration of 500 µg/disk in 48 hours of 4.95 mm and 5.1 mm. The bioactive compounds contained in the methanol extract of K. alvarezii are alkaloids, flavonoids, steroids and tannins.  ","PeriodicalId":50153,"journal":{"name":"Journal of Marine Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139868678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Senyawa Bioaktif Kappaphycus alvarezii (Doty) Doty, 1988 Sebagai Antibakteri","authors":"Annisa Marhaeny, Rini Pramesti, S. Suryono","doi":"10.14710/jmr.v13i1.40459","DOIUrl":"https://doi.org/10.14710/jmr.v13i1.40459","url":null,"abstract":"Bakteri yang menyebabkan penyakit pada inangnya disebut patogen. Penelitian ini bertujuan mengetahui senyawa bioaktif dan aktivitas antibakteri yang terkandung dalam ekstrak metanol K. alvarezii. Penelitian dilakukan dengan metode deskriptif eksploratif. Bakteri yang diujikan yaitu Staphylococcus aureus dan Escherichia coli. Hasil ekstraksi dilanjutkan dengan uji fitokimia. Ekstrak diencerkan menggunakan dimetil sulfoksida (DMSO) sampai diperoleh konsentrasi uji 500 µg/disk, 1000 µg/disk, dan 1500 µg/disk. Cefadroxil dan DMSO digunakan sebagai kontrol positif dan kontrol negatif. Metode difusi cakram digunakan untuk uji aktivitas antibakteri. Inokulum diinkubasi selama 3x24 jam pada suhu 37ºC. Zona hambat dihitung setiap 24 jam sekali. Hasil uji aktivitas antibakteri ekstrak metanol K. alvarezii terhadap bakteri S. aureus dan E. coli menunjukkan zona hambat terbesar pada konsentrasi 500 µg/disk pada waktu 48 jam sebesar 4,95 mm dan 5,1 mm. Senyawa bioaktif yang terkandung dalam ekstrak metanol K. alvarezii yaitu senyawa alkaloid, flavonoid, steroid dan tanin. Bacteria that cause disease in their hosts are called pathogens. This study aims to determine the bioactive compounds and antibacterial activity in the methanol extract of K. alvarezii. The research was conducted using a exploratory descriptive method. The bacteria tested were Staphylococcus aureus and Escherichia coli. Extraction then phytochemical tests were carried out The extract was diluted using dimethyl sulfoxide (DMSO) to obtain test concentrations of 500 µg/disk, 1000 µg/disk, and 1500 µg/disk. Cefadroxil and DMSO were used as positive and negative controls. Antibacterial activity test using disc diffusion method. The inoculum was incubated for 3x24 hours at 37°C. The inhibition zone is calculated every 24 hours. The results of the antibacterial activity test of K. alvarezii methanol extract against S. aureus and E. coli bacteria showed the largest inhibition zone at a concentration of 500 µg/disk in 48 hours of 4.95 mm and 5.1 mm. The bioactive compounds contained in the methanol extract of K. alvarezii are alkaloids, flavonoids, steroids and tannins.  ","PeriodicalId":50153,"journal":{"name":"Journal of Marine Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139808584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Suplementasi Ekstrak Spirulina sp. Pada Pakan Meningkatkan Toleransi Post-Larva L. vannamei Terhadap Stres Salinitas","authors":"Raisha Fahmida Dwi Lestari, Ervia Yudiati, Ali Djunaedi","doi":"10.14710/jmr.v13i1.37701","DOIUrl":"https://doi.org/10.14710/jmr.v13i1.37701","url":null,"abstract":"Litopenaeus vannamei merupakan komoditas yang bernilai ekonomi tinggi pada sektor perikanan dan budidaya. Spirulina sp. merupakan mikroalga yang mengandung nutrisi tinggi dan senyawa potensial yang memiliki efek terapeutik. Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi ekstrak Spirulina sp. dalam pakan terhadap tingkat kelangsungan hidup, pertumbuhan, dan tingkat kelangsungan hidup L. vannamei pada uji stres salinitas. Hewan uji yang digunakan adalah post-larvae (PL 8) L. vannamei dengan bobot rata-rata 0,002 g/ekor. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri atas 3 konsentrasi, yaitu 3 mg/kg (SP 3), 6 mg/kg (SP 6), dan 9 mg/kg (SP 9) serta satu kontrol dengan masing-masing 4 pengulangan. L. vannamei dipelihara dan diberi pakan suplementasi Spirulina sp. selama 14 hari. Pada akhir penelitian dilakukan uji stres salinitas dengan menggunakan tabung falcon yang masing-masing diisi 20 ekor PL dan dipaparkan pada akuades (45 mL) dan ditutup rapat. PL diamati dan dihitung jumlah kelulushidupannya setiap 10 menit. Waktu pengamatan dihentikan ketika kematian udang mencapai 50%.Hasil penelitian menunjukkan bahwa pemberian suplementasi ekstrak Spirulina sp. pada pakan tidak berpengaruh nyata (p>0,05) terhadap pertumbuhan dan tingkat kelangsungan hidup udang. Namun, perlakuan SP 9 menunjukkan pengaruh nyata (p<0,05) terhadap tingkat kelangsungan pada uji stres salinitas. Suplementasi ekstrak Spirulina sp. mampu meningkatkan toleransi L. vannamei terhadap stres salinitas.  Litopenaeus vannamei is high economically viable cultivate in the aquaculture sectors. Spirulina sp. is high nutrients microalgae and potential compound that have therapeutic effects. This study aims to determine the effect of dietary supplementation of Spirulina sp. on feed towards survival rate, growth, and resistance of L. vannamei in the salinity stress test. This experiment was using post-larvae (PL 8) (0.002 g/ind). This study used a completely randomized design (CRD) consisting of 3 concentrations, namely 3 mg/kg (SP 3), 6 mg/kg (SP 6), and 9 mg/kg (SP 9), and one control with 4 repetitions for each treatment.  L. vannamei was reared and fed with Spirulina sp. supplementation for 14 days. On stress salinity test, PL were put in the falcon tube filled with aqua dest with 20 PL each and exposed until 50% mortality. The results showed that the supplementation of Spirulina sp. on feed had no significant effect (p>0.05) on the growth and survival rate of shrimp. However, the SP 9 treatment showed a significant effect (p<0.05) on the survival rate in the salinity stress test. Is concluded that supplementation of Spirulina sp. in the diet managed to increase the resistance of L. vannamei toward stress salinity.","PeriodicalId":50153,"journal":{"name":"Journal of Marine Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140510838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analisis Kualitas Air dan Kandungan Logam Berat Timbal (Pb) Pada Air Laut di Perairan Pelabuhan Tanjung Emas Kota Semarang Jawa Tengah","authors":"Anggita Al Haris Sulistyo, Jusup Suprijanto, Bambang Yulianto","doi":"10.14710/jmr.v13i1.38751","DOIUrl":"https://doi.org/10.14710/jmr.v13i1.38751","url":null,"abstract":"Pelabuhan Tanjung Emas, Semarang, Jawa Tengah termasuk ke dalam salah satu fasilitas pelabuhan di Indonesia yang menaungi beberapa industri di dalamnya. Lokasi penelitian kali ini bertempat di salah satu fasilitas pelabuhan yaitu Dermaga Nusantara yang secara umum memiliki aktivitas pelabuhan seperti bongkar muat batu bara, pasir, dan log kayu menggunakan alat berat seperti Exavator dan Wheel Loader. Adanya banyak aktivitas pelabuhan dapat dikatakan sebagai faktor yang dapat mempengaruhi keadaan atau baik buruknya air laut serta kadar timbal (Pb) pada air laut di area Dermaga Nusantara. Penelitian ini dilakukan untuk mengetahui kualitas air laut, menganalisis kadar logam berat pada air laut dan menganalisis tindakan pencegahan yang dapat dilakukan untuk meminimalisir timbulnya pencemaran laut. Analisis kualitas perairan menggunakan alat ukur thermometer digital, pH meter, DO meter, secchi disk, dan refractometer. Pengujian kandungan timbal (Pb) menggunakan alat AAS dilakukan di Laboratorium Teknik Lingkungan Universitas Diponegoro. Hasil pengukuran suhu pada kisaran 30,5-32,2°C; nilai pH pada kisaran 6,35-7,56; nilai dissolved oxygen (DO) pada kisaran 5,22-8,51 mg/l; nilai kecerahan pada kisaran 3,18-3,52 meter; dan nilai salinitas pada kisaran 31-35 ppt. Hasil uji kandungan timbal (Pb) yaitu pada kisaran 0,390 – 0,640 mg/l. Nilai pengukuran kualitas air tersebut masih tergolong memenuhi standar baku mutu, akan tetapi pada uji kandungan timbal hasil yang didapatkan masih melebihi standar baku mutu sesuai dengan PP Nomor 22 Tahun 2021 Republik Indonesia mengenai Penyelenggaraan Perlindungan dan Pengelolaan Lingkungan Hidup dan KepMen LH Nomor 51 Tahun 2004 mengenai Baku Mutu Air Laut Pelabuhan.  Tanjung Emas Port, Semarang, Central Java is included in one of the port facilities in Indonesia which houses several industries in it. The research location this time is located at one of the port facilities, namely the Nusantara Jetty which generally has port activities such as loading and unloading of coal, sand, and wood logs using heavy equipment such as excavators and wheel loaders. The existence of many port activities can be regarded as a factor that can influence the condition or good and bad of sea water and levels of lead (Pb) in sea water in the Nusantara Pier area. This research was conducted to determine the quality of seawater, analyze levels of heavy metals in seawater and analyze preventive measures that can be taken to minimize marine pollution. Analysis of water quality using digital thermometers, pH meters, DO meters, secchi disks, and refractometers. Testing for lead (Pb) content using the AAS tool was carried out at the Diponegoro University Environmental Engineering Laboratory. Temperature measurement results in the range of 30.5-32.2°C; pH value in the range of 6.35-7.56; dissolved oxygen (DO) value in the range of 5.22-8.51 mg/l; brightness value in the range of 3.18-3.52 meters; and salinity values in the range of 31-35 ppt. The t","PeriodicalId":50153,"journal":{"name":"Journal of Marine Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140510309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kajian Persentase Tutupan Kanopi Mangrove Menggunakan Metode Hemispherical Photography di Desa Sambiroto dan Desa Keboromo, Kabupaten Pati, Jawa Tengah","authors":"Muhamad Fajar Azmi Kassagi, R. Ario, Nirwani Soenardjo","doi":"10.14710/jmr.v13i1.35424","DOIUrl":"https://doi.org/10.14710/jmr.v13i1.35424","url":null,"abstract":"Ekosistem mangrove merupakan komunitas vegetasi pantai tropis dan subtropis yang   dapat tumbuh dan beradaptasi pada daerah pasang surut. Keberadaan mangrove penting sebagai sumber nutrient bagi kelangsungan hidup biota di dalamnya. Penelitian ini bertujuan untuk mengetahui kondisi ekosistem mangrove di Desa Sambiroto dan Desa Keboromo, Kabupaten Pati, Jawa Tengah melalui analisis persentase tutupan kanopi mangrove. Stasiun pengamatan terbagi mejadi tiga stasiun mengunakan purposive sampling. Penelitian ini dilakukan dengan metode deskriptif. Penelitian dilakukan di 2 lokasi, yaitu Desa Sambiroto (SB) dan Desa Keboromo (KB) yang terdiri dari 4 stasiun penelitian. Pengambilan data persentase tutupan kanopi mangrove mengunakan metode Hemispherical Photography. Hemispherical Photography merupakan metode fotografi yang digunakan untuk melihat tutupan kanopi mangrove melalui foto dengan lensa fisheye (kamera HP). Hasil penelitian menunjukkan bahwa ditemukan 3 spesies mangrove di lokasi penelitian, 2 diantaranya di temukan di dalam plot. Spesies mangrove yang ditemukan di dalam plot yaitu Aviccenia marina dan A. alba yang di dominasi oleh A. marina. Nilai kerapatan mangrove berkisar antara 1.167 – 6.567 ind/ha .Nilai Indeks Keanekaragaman (H’) dan Indeks Keseragaman (J’) termasuk rendah, Indeks Dominansi (D) di setiap stasiun >0,5 menunjukkan adanya kecenderungan dominansi jenis tertentu. Hasil persentase tutupan kanopi mangrove di Desa Sambiroto dan Desa Keboromo memiliki nilai sebesar 67,61 % dan termasuk ke dalam kategori sedang sesuai kriteria baku kerusakan mangrove (Keputusan Menteri LH No.201 Tahun 2004). The existence of mangrove forests is very important as a source of nutrients for the survival of the biota in it. This study aims to determine the condition of the mangrove ecosystem in Sambiroto and Keboromo Village, Pati Regency, Central Java through analysis of the percentage of mangrove canopy cover. The observation stations were divided into three stations using purposive sampling. This research was conducted by descriptive method. This research was conducte in 2 research locations, Sambiroto Village (SB) and Keboromo Village (KB), which consisted of 4 research stations. Data collection on the percentage of mangrove canopy cover using the Hemispherical Photography method. Hemispherical photography is a photography method used to see the mangrove canopy cover through photos with a fish eye lens (HP camera). The result showed that 6 species of mangrove were found in the study site, 2 of which were found in the plot. The mangrove species found in the plot were Avicennia marina and Avicennia alba which were dominated by Avicennia marina. The value of mangrove density ranges from 1.167 – 6.567 ind/ha. The values of the Diversity Index (H’) and the Uniformity Index (J’) are low, the Dominance Index (D) at eatch station >0.5 indicates a tendency for the dominance of certain species. The results of the percentage of mangrove canopy cover in Sambir","PeriodicalId":50153,"journal":{"name":"Journal of Marine Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140510770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tingkat Keberhasilan Penetasan Telur Penyu Hijau (Chelonia mydas) dan Penyu Sisik (Eretmochelys imbricata) Di Pulau Mangkai Kepulauan Anambas","authors":"Ronald Raditya Kesatria Sinaga, Andriyatno Hanif, F. Kurniawan, Syofyan Roni, Dominikus Yoeli Wilson Laia, Jelita Rahma Hidayati","doi":"10.14710/jmr.v13i1.38531","DOIUrl":"https://doi.org/10.14710/jmr.v13i1.38531","url":null,"abstract":"Penyu adalah biota yang termasuk kategori hewan dilindungi, karena memiliki tingkat keterancaman punah yang tinggi dari faktor alami maupun antropogenik. Penyu menghabiskan hidupnya di laut lepas dan pergi ke daratan untuk bertelur pada setiap musimnya. Keberhasilan penetasan telur penyu menjadi hal yang penting sebagai upaya untuk menambah populasinya. Penelitian ini bertujuan untuk menganalisis keberhasilan daya tetas telur penyu hijau (Chelonia mydas) serta penyu sisik (Eretmochelys imbricata) di Pulau Mangkai Kabupaten Kepulauan Anambas. Penelitian dilakukan pada tahun 2022 di bagian utara (Pantai Empung) dan selatan Pulau Mangkai (Pantai Pantai Suit, Pantai Skanji dan Pantai Teluk Kado). Penelitian dilakukan dengan melakukan pengamatan pada kondisi pantai pendaratan, jumlah sarang, jumlah telur, probabilitas dan frekuensi penyu bertelur, persentase telur menetas, hatching success dan persentase tukik hidup dan rilis. Hasil penelitian menunjukkan bahwa terdapat dua jenis penyu yaitu penyu hijau (Chelonia mydas) dan penyu sisik (Eretmochelys imbricata) yang melakukan pendaratan di Pulau Mangkai. Probabilitas penyu bertelur terjadi selama 8 bulan dan probabilitas tertinggi didapat pada bulan Juni (100 %). Terdapat 615 sarang semi alami dan 4 sarang alami, di mana jumlah sarang tertinggi berada pada bulan Juni dengan total 164 sarang dari jenis penyu hijau. Jumlah telur yang ditemukan sebanyak 55.916 butir dan total telur yang menetas sebanyak 48.864 telur penyu. Rata-rata persentase hatching success didapatkan sebesar 86.27%, rata-rata persentase tukik hidup dan rilis sebesar 99.2%. Total tukik hidup dan yang berhasil dirilis di Pulau Mangkai sebanyak 48.276 ekor.  Sea turtles are one of the protected biota species because they have a high level of endangerment from both natural and anthropogenic factors. Turtles spend their lives on the high seas and go ashore to lay their eggs every season. The success of hatching turtle eggs is essential to increase their population. This study analyzes the hatching success rates of green sea turtles (Chelonia mydas) and hawksbill sea turtles (Eretmochelys imbricata) on Mangkai Island, Anambas Islands Regency. The research was conducted in 2022 in the northern (Empung Beach) and southern parts of Mangkai Island (Suit Beach, Skanji Beach, and Teluk Kado Beach). The research was conducted by observing the conditions of the number of nests, the number of eggs, the probability and frequency of turtle laying eggs, the percentage of hatching eggs, hatching success, and the rate of live and released hatchlings. The results showed that two types of turtles, namely the green sea turtle (Chelonia mydas) and the hawksbill sea turtle (Eretmochelys imbricata), landed on Mangkai Island. The probability of laying eggs occurs for eight months, and the highest probability is obtained in June (100%). There were 615 semi-natural nests and 4 natural nests, where the highest number was in June, with a total of 164 nests of green","PeriodicalId":50153,"journal":{"name":"Journal of Marine Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140510259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analisis Kandungan Klorofil -a dan Kepadatan Diatom Thalassiosira sp. Dengan Penggunaan Konsentrasi Silikat yang Berbeda","authors":"Syahrial Varrel Cannavaro, Hadi Endrawati, W. A. Setyati","doi":"10.14710/jmr.v13i1.35303","DOIUrl":"https://doi.org/10.14710/jmr.v13i1.35303","url":null,"abstract":"Thalassiosira sp. adalah diatom yang digunakan sebagai pakan alami bagi larva udang vanamei. Thalassiosira sp. mempunyai kandungan protein sekitar 44,5 %,karbohidrat 26,1% dan lemak 11,8% dari berat keringnya. Thalassiosira sp. adalah salah satu pakan alami yang baik karena mempunyai nilai nutrisi yang tinngi serta kandungan silika yang bagus untuk larva udang vanamei. Pertumbuhan diatom dibutuhkan media nutrisi dalam mendukung pertumbuhan plankton harus mengandung silika untuk mendukung pertumbuhan dan memperkuat cangkang sel. Penelitian ini bertujuan untuk mengetahui konsentrasi silikat yang tepat guna mengoptimalkan pertumbuhan kepadatan dan produksi pigmen klorofil pada Thalasiosira sp. Metode yang digunakan adalah eksperimen laboratorium dengan menggunakan Rancangan Acak Lengkap. Mikroalga Thalassiosira sp di kultivasi dengan lima taraf perlakuan konsentrasi silikat yang berbeda yaitu 0 ppm, 12 ppm,15 ppm, 18 ppm dan 21 ppm dengan tiga ulangan. Pertumbuhan Thalasiosira sp. diamati selama 13 x 24 jam kemudian dipanen untuk perhitungan biomassanya. Biomassa basah hasil kultivasi diekstraksi menggunakan pelarut aseton PA. Ekstrak aseton Thalassiosira sp. kemudian dianalisis kandungan pigmen klorofilnya dengan spektrofotometri. Hasil densitas tertinggi terdapat pada konsentrasi (15 ppm) Kandungan Klorofil Thalassiosira sp. tertinggi diproduksi pada konsentrasi (15 ppm) yaitu 184 µg/mL. Berdasarkan hasil penelitian dapat disimpulkan bahwa konsentrasi silikat berpengaruh secara signifikan terhadap kandungan klorofil Thalassiosira sp. Thalassiosira sp is a diatom that is used as natural food for vanamei shrimp larvae. Thalassiosira sp. It has a protein content of about 44.5%, carbohydrates 26.1% and fat 11.8% of its dry weight. Thalassiosira sp. is one of the good natural feeds because it has high nutritional value and good silica content for vanamei shrimp larvae. The growth of diatoms requires a nutrient medium to support the growth of plankton. It must contain silica to support growth and strengthen cell shells. This study aims to determine the appropriate concentration of silicate in order to optimize the growth of density and production of chlorophyll pigment in Thalasiosira sp. The method used is a laboratory experiment using a completely randomized design. The microalgae Thalasiosira sp was cultivated with five different levels of silicate concentration treatment, namely 0 ppm, 12 ppm, 15 ppm, 18 ppm and 21 ppm with three replications. Growth of Thalasiosira sp. observed for 13 x 24 hours and then harvested for biomass calculation. The wet biomass from the cultivation was extracted using acetone PA as a solvent. Thalasiosira sp. acetone extract. then analyzed the content of chlorophyll pigment by spectrophotometry. The highest density results were found at the concentration (15 ppm) of the Chlorophyll content of Thalassiosira sp. The highest was produced at a concentration (15 ppm) which was 184 g/mL. Based on the results of the study, it ca","PeriodicalId":50153,"journal":{"name":"Journal of Marine Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140510370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Karakteristik Sarang Peneluran Penyu Lekang (Lepidochelys olivacea) di Pantai Jeen Yessa, Papua Barat - Indonesia","authors":"Arifatul Hasanah, Duaitd Kolibongso, Deasy Lontoh","doi":"10.14710/jmr.v13i1.38103","DOIUrl":"https://doi.org/10.14710/jmr.v13i1.38103","url":null,"abstract":"Pantai Jeen Yessa merupakan salah satu tempat bertelur bagi beberapa spesies penyu di Pasifik Barat, dengan sekitar 75% peneluran terjadi di pantai utara semenanjung Bentang Laut Kepala Burung di wilayah Abun, Papua Barat. Wilayah ini memiliki karakteristik suhu yang tinggi dan kelembaban yang rendah. Kedua faktor tersebut memainkan peran penting dalam keberhasilan reproduksi. Penelitian ini merupakan salah satu studi pertama mengenai karakteristik morfologi sarang penyu lekang (L. olivacea) di Pantai Jeen Yessa, dan diharapkan dapat memberikan kontribusi terhadap pengetahuan regional mengenai spesies ini serta membantu dalam pengelolaan perlindungan setempat. Survei ini dilakukan di tiga lokasi selama musim bertelur pada Mei-Juli 2022. Karakteristik sarang L. olivacea: rata-rata bagian atas sarang 30 ± 5,41 (rata-rata ± SD) cm (kisaran 20 - 43 cm, n = 34); bagian bawah sarang 42 ± 5,14 (rata-rata ± SD) cm (kisaran 32 - 54 cm, n = 34); dan lebar sarang 27,3 ± 4,87 (rata-rata ± SD) cm (kisaran 18 - 38 cm, n = 34). Masa inkubasi antara kedua lokasi bervariasi: Warmamedi 62,5 hari ± 4,5 dan Batu Rumah 48,7 hari ± 1,8 (rata-rata ± SD). Rata-rata keberhasilan penetasan adalah 78,3%, dan tingkat kematian 21,7% (jumlah telur yang dihitung = 2.367). Penelitian lebih lanjut mengenai metode inkubasi buatan sangat disarankan, karena ini bisa menjadi pilihan penting untuk melestarikan populasi penyu.  Jeen Yessa Beach is one of the nesting sites for several turtle species in Western Pasific, with approximately 75% of nesting occurring on the northern coast of the Bird’s Head Seascape peninsula in the Abun region of West Papua. This region is characterized by high temperatures and low humidity. Both factors play an important role in reproductive success. This is one of the first studies on the morphological characteristics of the nest of the olive ridley turtle (L. olivacea) on the Jeen Yessa beach, and it is expected to contribute to regional knowledge of this species and aid in local protection management. The survey was conducted in three locations during the nesting season in Mei-July 2022. L. olivacea nest characteristics: average nest top 30 ± 5.41 (mean ± SD) cm (range 20 – 43 cm, n = 34); nest bottom 42 ± 5.14 (mean ± SD) cm (range 32-54 cm, n = 34); and a nest width of 27.3 ± 4.9 (mean ± SD) cm (range 18 – 38 cm, n = 34). The incubation period between the two sites varies: Warmamedi 62.5 days ± 4.5 and Batu Rumah 48.7 days ± 1.8 (mean ± SD). The average hatching success was 78.3%, and the mortality rate was 21.7% (eggs counted = 2,367). More research into artificial incubation methods is recommended, as this could be an important option for preserving some sea turtle populations.","PeriodicalId":50153,"journal":{"name":"Journal of Marine Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140510450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopatologi Organ Ikan Kerapu Bebek (Cromileptes altivelis) sebagai Bioindikator Perairan Teluk Kupang, NTT","authors":"S. J. Juanda, Aisyah Lukmini, Ihsan Sanggar Rahman, M. F. Panuntun","doi":"10.14710/jmr.v13i1.41175","DOIUrl":"https://doi.org/10.14710/jmr.v13i1.41175","url":null,"abstract":"Saat ini, informasi tentang respon fisiologis biota akuatik yang hidup di perairan Teluk Kupang masih sangat minim. Penelitian ini bertujuan untuk mengidentifikasi, menghitung intensitas dan prevalensi parasit serta menganalisis kerusakan organ pada kerapu bebek yang diambil dari perairan Teluk Kupang. Penelitian ini dilaksanakan selama bulan Juli 2023. Sampel ikan yang digunakan adalah ikan kerapu hidup yang ditangkap oleh nelayan, selanjutnya Ikan dibawa ke laboratorium untuk diamati gejala klinis morfologinya yang meliputi bentuk, kondisi tubuh ikan, warna, sirip, sisik dan insang. Pengamatan ektoparasit dilakukan pada lendir tubuh, sirip dan insang, sedangkan endoparasit diambil pada usus ikan. Preparasi histologi dilakukan pada organ insang dan usus ikan. Analisis histopatologi dilakukan dengan pengamatan secara mikroskopik pada masing-masing organ selanjutnya dibandingkan dengan gambaran histopatologi yang didapatkan dari referensi jurnal yang relevan yang mencakup struktur jaringan mikroskopik dari organ serta kerusakannya. Pada penelitian ini tidak ditemukan adanya gejala klinis morfologi, namun pada pengamatan ektoparasit dan endoparasit ditemukan parasit yang menginfestasi yaitu Anisakis sp. Intensitas ektoparasit Anisakis sp 0,67 ind/ekor yang artinya infeksi sangat rendah dengan nilai prevalensi 57,14% yang termasuk infeksi sangat sering. Nilai intensitas endoparasit sebesar 1 ind/ekor yang artinya infeksi rendah dan prevalensi 85,71% yang termasuk infeksi serangan sedang. Beberapa kerusakan jaringan insang yang ditemukan antara lain edema lamela primer dan sekunder, proliferasi sel mukus, clubbing, fusi lamela primer dan sekunder, adanya vakuola, telangiektasis, hiperplasia lamela sekunder, degenerasi lamela sekunder, lisis, kemunculan protozoa dan parasit. Sedangkan kerusakan pada usus yaitu nekrosis, edema, erosi vili, perlemakan, hemoragi, lisis, adanya vakuola dan kemunculan parasit dan protozoa.   Currently, the information about physiological responses of aquatic biota in Kupang Bay is still very minimal. This research aims to identify, calculate the intensity and prevalence of parasites and organ damage analyzed in Barramundi Cod Grouper taken from Kupang Bay. This research was carried out during July 2023. The sample of fishes caught by fisherman and taken to the laboratory to be observed for clinical morphological symptoms, such us shape, fish’s body, color, fins, scales and gills. Ectoparasites were observed in the body mucus, fins and gills, while endoparasites in the intestines. Histological preparastions were carried out on the gills and intestines. Histopathological analysis were carried out by microscopic observation of each organ and then compared with the image obtained from relevant journal references which includes the microscopic tissue structure and its damages. In this study, no morphological clinical symptoms were found, however, when observing ectoparasites and endoparasites, parasities was found, namely Anisa","PeriodicalId":50153,"journal":{"name":"Journal of Marine Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140510226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deteksi Total Bakteri Escherichia coli Pada Sedimen Laut Perairan Desa Padelegan Sebagai Indikator Cemaran Mikrobiologis Wisata Pantai The Legend-Pamekasan","authors":"Eka Nurahemma Ning Asih, A. Ramadhanti, Ashari Wicaksono, Kartika Dewi, Sri Astutik","doi":"10.14710/jmr.v13i1.37063","DOIUrl":"https://doi.org/10.14710/jmr.v13i1.37063","url":null,"abstract":"Escherichia coli tergolong bakteri fecal coliform yang merupakan kelompok bakteri patogen sebagai indikator pencemaran mikrobiologi air. Tingginya jumlah bakteri tersebut di perairan Desa Padelegan diduga disebabkan oleh tekanan pencemaran dalam rumah tangga. Penelitian ini dilakukan untuk mengetahui kualitas bakteriologis khususnya bakteri Escherichia coli yang terakumulasi pada sedimen perairan Desa Padelegan terkait dengan keselamatan operasional kawasan wisata pantai The Legend di sekitar lokasi. Metode yang digunakan untuk analisis total bakteri Escherichia coli mengacu pada SNI 2332.1:2015. Hasil penelitian menunjukkan nilai total bakteri Escherichia coli pada 7 stasiun berkisar antara <3,0 MPN/g - 93 MPN/g. Total bakteri Escherichia coli pada seluruh stasiun tersebut berada di bawah ambang batas untuk keperluan wisata bahari sesuai Keputusan Menteri Negara Lingkungan Hidup No. 51 Tahun 2004 yaitu <200 MPN/g dengan toleransi <10%. Nilai analisis total bakteri Escherichia coli menunjukkan bahwa kualitas perairan khususnya sedimen di sekitar Desa Padelegan berada pada kategori masih baik dan aman untuk kegiatan wisata bahari. Escherichia coli is classified as Fecal coliform bacteria which is a group of pathogenic bacteria as an indicator of water microbiological contamination. The high total number of these bacteria in the waters of Padelegan Village is thought to have come from the pressure of domestic contamination. This study was conducted to determine the bacteriological quality, especially Escherichia coli bacteria that accumulate in the sediments of the waters of Padelegan Village related to the safety of operating beach tourism areas The Legend around the location. The method used for the total analysis of Escherichia coli bacteria refers to SNI 2332.1:2015. The results showed that the total value of Escherichia coli bacteria at 7 stations ranged from <3.0 MPN/g - 93 MPN/g. The total Escherichia coli bacteria at all of these stations were below the threshold for marine tourism purposes according to the Decree of the State Minister for the Environment No. 51 of 2004, namely <200 MPN/g with a tolerance of <10%. The value of the total analysis of Escherichia coli bacteria shows that the quality of the waters, especially the sediments around the village of Padelegan, is in the category that is still good and safe for marine tourism activities.","PeriodicalId":50153,"journal":{"name":"Journal of Marine Research","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140509902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}